gnr1 (SPCC1020.09)


Gene Standard Namegnr1 Characterisation Statuspublished
Systematic IDSPCC1020.09 Feature Typeprotein coding
Synonyms Name DescriptionG protein Negative Regulator
Productheterotrimeric G protein beta subunit Gnr1 Product Size399aa, 45.75 kDa
Genomic Location Chromosome III, 767541-765936 (1606nt); CDS:767369-766170 (1200nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
signal transducer activity29
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
heterotrimeric G-protein complex5
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
viable vegetative cell populationgnr1ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
decreased transcriptional response to pheromonegnr1+ (wild type)Overexpression5
increased transcriptional response to pheromonegnr1ΔNull3
viable vegetative cell with normal cell morphologygnr1ΔNull3094
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons767541..765936
mRNA767541..765936
5' UTR767541..767370PMID:21511999
CDS767369..766170
3' UTR766169..765936PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
SM00320 SMART IPR001680 WD40 repeat 39 74 112
SM00320 SMART IPR001680 WD40 repeat 139 182 112
SM00320 SMART IPR001680 WD40 repeat 100 136 112
SM00320 SMART IPR001680 WD40 repeat 201 238 112
SM00320 SMART IPR001680 WD40 repeat 287 328 112
SM00320 SMART IPR001680 WD40 repeat 244 283 112
PTHR13211 HMMPANTHER 30 398 1
2.130.10.10 Gene3D IPR015943 WD40/YVTN repeat-like-containing domain 49 371 145
SSF50978 SuperFamily IPR017986 WD40-repeat-containing domain 50 370 135

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000190WD repeat proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000190

Protein Properties

Ave. residue weight 114.66 Da
Charge -2.00
Isoelectric point 6.24
Molecular weight 45.75 kDa
Number of residues 399
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
360.11during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.93during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.34during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog599
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withado1adenosine kinase (predicted) Negative GeneticPMID:22681890
positive genetic interaction withamo1nuclear rim protein Amo1 Positive GeneticPMID:18818364
positive genetic interaction withcbp1CENP-B homolog Positive GeneticPMID:18818364
positive genetic interaction withcmk1calcium/calmodulin-dependent protein kinase Cmk1 Positive GeneticPMID:22681890
positive genetic interaction withcsk1cyclin-dependent kinase/ cyclin-dependent kinase activating kinase Csk1 Positive GeneticPMID:22681890
positive genetic interaction withdad2DASH complex subunit Dad2 Positive GeneticPMID:18818364
negative genetic interaction withdsk1SR protein-specific kinase Dsk1 Negative GeneticPMID:22681890
negative genetic interaction withget1GET complex subunit Get1 (predicted) Negative GeneticPMID:18818364
rescued bygit5heterotrimeric G protein beta subunit Git5 Phenotypic SuppressionPMID:16884933
positive genetic interaction withgmh5alpha-1,2-galactosyltransferase (predicted) Positive GeneticPMID:18818364
positive genetic interaction withhrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Positive GeneticPMID:18818364
positive genetic interaction withmcl1DNA polymerase alpha accessory factor Mcl1 Positive GeneticPMID:18818364
negative genetic interaction withmed20mediator complex subunit Med20 Negative GeneticPMID:18818364
positive genetic interaction withpck1protein kinase C (PKC)-like Pck1 Positive GeneticPMID:22681890
positive genetic interaction withppk16serine/threonine protein kinase Ppk16 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withppk31serine/threonine protein kinase Ppk31 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withptn1phosphatidylinositol-3,4,5-trisphosphate3-phosphatase Ptn1 Positive GeneticPMID:18818364
negative genetic interaction withraf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Negative GeneticPMID:22681890
negative genetic interaction withrav2RAVE complex subunit Rav2 Negative GeneticPMID:22681890
positive genetic interaction withrps40240S ribosomal protein S4 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withryh1GTPase Ryh1 Positive GeneticPMID:18818364
negative genetic interaction withSPAC17G6.03phosphoprotein phosphatase (predicted) Negative GeneticPMID:18818364
negative genetic interaction withSPAC18G6.13Schizosaccharomyces specific protein Negative GeneticPMID:22681890
negative genetic interaction withspf31DNAJ protein Spf31 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withtrp663TRP-like ion channel (predicted) Negative GeneticPMID:22681890
negative genetic interaction withuap2U2 snRNP-associated protein Uap2 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPCC1020.09 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1020.09 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1020.09 BioGRID Interaction Datasets
Expression Viewer SPCC1020.09 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1020.09 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1020.09 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1020.09 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1020.09 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1020.09 Transcriptome Viewer (Bähler Lab)
GEO SPCC1020.09 GEO profiles
PInt SPCC1020.09 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC1020.09 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC1020.09 Fission yeast phenotypic data & analysis
Cyclebase SPCC1020.09.1 Cell Cycle Data
SPD / RIKEN44/44A05Orfeome Localization Data
UniProtKB/SwissProtO59762Guanine nucleotide-binding protein negative regulator 1
ModBaseO59762Database of comparative protein structure models
STRINGO59762Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587950heterotrimeric G protein beta subunit Gnr1
RefSeq mRNANM_001022941972h- heterotrimeric G protein beta subunit Gnr1 (gnr1), mRNA
European Nucleotide ArchiveCAA18997.1ENA Protein Mapping
UniParcUPI000006BDF2UniProt Archive

Literature for gnr1

Search: Europe PMC or PubMed

Release Version: PomBase:26_53 - 27 May 2015