gnr1 (SPCC1020.09)


Gene Standard Namegnr1 Characterisation Statuspublished
Systematic IDSPCC1020.09 Feature Typeprotein coding
Synonyms Name DescriptionG protein Negative Regulator
Productheterotrimeric G protein beta subunit Gnr1 Product Size399aa, 45.75 kDa
Genomic Location Chromosome III, 767541-765936 (1606nt); CDS:767369-766170 (1200nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005515protein bindingIPIgpa1PMID:16884933865
IPIgpa1PMID:16884933
GO:0004871signal transducer activity29
contributes_toTASPMID:16884933
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0045744negative regulation of G-protein coupled receptor protein signaling pathwayIMPPMID:168849338
GO:0090029negative regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusionIMPPMID:168849334
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722319
GO:0005834heterotrimeric G-protein complexIPIgpa1PMID:168849335
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopygnr1ΔNullPMID:126183703751
Microscopygnr1ΔNullPMID:20473289
Microscopygnr1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000302abnormal response to pheromoneReporter gene assaygnr1-ΔNullPMID:1688493319
FYPO:0000781decreased transcription during vegetative growthReporter gene assaynot recorded (wild type)OverexpressionPMID:1688493320
FYPO:0000987decreased transcriptional response to pheromone at normal pheromone levelReporter gene assaygnr1+ (wild type)OverexpressionPMID:168849333
FYPO:0000685transcriptional response to pheromone at decreased pheromone levelReporter gene assaygnr1-ΔNullPMID:168849333
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopygnr1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1767541765936
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
SM00320 SMART IPR001680 WD40 repeat 39 74 112
SM00320 SMART IPR001680 WD40 repeat 100 136 112
SM00320 SMART IPR001680 WD40 repeat 139 182 112
SM00320 SMART IPR001680 WD40 repeat 201 238 112
SM00320 SMART IPR001680 WD40 repeat 287 328 112
SM00320 SMART IPR001680 WD40 repeat 244 283 112
PTHR13211 HMMPANTHER 22 398 1
2.130.10.10 Gene3D IPR015943 WD40/YVTN repeat-like-containing domain 49 371 145
SSF50978 SuperFamily IPR017986 WD40-repeat-containing domain 50 370 135

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000190WD repeat proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000190

Protein Properties

Ave. residue weight 114.66 Da
Charge -2.00
Isoelectric point 6.24
Molecular weight 45.75 kDa
Number of residues 399
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
360.11during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.93during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.34during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog601
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
med20mediator complex subunit Med20 Negative GeneticPMID:18818364
cbp1CENP-B homolog Positive GeneticPMID:18818364
ppk16serine/threonine protein kinase Ppk16 (predicted) Positive GeneticPMID:22681890
cmk1calcium/calmodulin-dependent protein kinase Cmk1 Positive GeneticPMID:22681890
get1GET complex subunit Get1 (predicted) Negative GeneticPMID:18818364
mcl1DNA polymerase alpha accessory factor Mcl1 Positive GeneticPMID:18818364
SPAC18G6.13Schizosaccharomyces specific protein Negative GeneticPMID:22681890
uap2U2 snRNP-associated protein Uap2 Negative GeneticPMID:22681890
gmh5alpha-1,2-galactosyltransferase (predicted) Positive GeneticPMID:18818364
pck1protein kinase C (PKC)-like Pck1 Positive GeneticPMID:22681890
ppk31serine/threonine protein kinase Ppk31 (predicted) Positive GeneticPMID:18818364
rav2RAVE complex subunit Rav2 Negative GeneticPMID:22681890
dad2DASH complex subunit Dad2 Positive GeneticPMID:18818364
dsk1SR protein-specific kinase Dsk1 Negative GeneticPMID:22681890
csk1cyclin-dependent kinase/ cyclin-dependent kinase activating kinase Csk1 Positive GeneticPMID:22681890
amo1nuclear rim protein Amo1 Positive GeneticPMID:18818364
ptn1phosphatidylinositol-3,4,5-trisphosphate3-phosphatase Ptn1 Positive GeneticPMID:18818364
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Positive GeneticPMID:18818364
rps40240S ribosomal protein S4 (predicted) Positive GeneticPMID:22681890
raf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Negative GeneticPMID:22681890
trp663TRP-like ion channel (predicted) Negative GeneticPMID:22681890
ado1adenosine kinase (predicted) Negative GeneticPMID:22681890
spf31DNAJ protein Spf31 (predicted) Negative GeneticPMID:22681890
ryh1GTPase Ryh1 Positive GeneticPMID:18818364
SPAC17G6.03phosphoprotein phosphatase (predicted) Negative GeneticPMID:18818364
External References
Database Identifier Description
NBRP SPCC1020.09 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1020.09 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1020.09 BioGRID Interaction Datasets
Expression Viewer SPCC1020.09 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1020.09 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1020.09 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1020.09 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1020.09 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1020.09 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC1020.09 Cell Cycle Data
GEO SPCC1020.09 GEO profiles
PInt SPCC1020.09 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC1020.09 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN44/44A05Orfeome Localization Data
UniProtKB/SwissProtO59762Guanine nucleotide-binding protein negative regulator 1
ModBaseO59762Database of comparative protein structure models
STRINGO59762Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587950heterotrimeric G protein beta subunit Gnr1
RefSeq mRNANM_001022941972h- heterotrimeric G protein beta subunit Gnr1 (gnr1), mRNA
European Nucleotide ArchiveCAA18997.1ENA Protein Mapping
UniParcUPI000006BDF2UniProt Archive

Literature for gnr1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014