ght5 (SPCC1235.14)


Gene Standard Nameght5 Characterisation Statuspublished
Systematic IDSPCC1235.14 Feature Typeprotein coding
Synonyms Name Description
Producthexose transmembrane transporter Ght5 Product Size546aa, 60.32 kDa
Genomic Location Chromosome III, 212160-214376 (2217nt); CDS:212313-213953 (1641nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
GO Cellular Component
Term NameCount
integral component of plasma membrane49
Annotation ExtensionEvidenceWith/FromReference
plasma membrane253
Annotation ExtensionEvidenceWith/FromReference
plasma membrane of cell tip18
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
abolished cell population growth during glucose starvationght5Δ4
normal growth on glucose carbon sourceght5Δ287
viable vegetative cell populationght5Δ3844

Cell Phenotype

Term NameGenotypesCount
abnormal high-affinity glucose importght5Δ4
viable vegetative cell with normal cell morphologyght5Δ3100

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased cell population growth on glucose carbon sourceght2Δ, ght8Δ, ght5Δ, ght1Δ
ght1Δ, ght5Δ, ght8Δ
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in scr1 transcription factor Scr1
FYPO affected by mutation in sds23 PP2A-type phosphatase inhibitor Sds23/Moc1
FYPO affected by mutation in ssp1 Ca2+/calmodulin-dependent (CaMMK)-like protein kinase Ssp1
FYPO localization affected by mutation in gad8 AGC family protein kinase Gad8
FYPO localization affected by mutation in ksg1 serine/threonine protein kinase Ksg1
FYPO localization affected by mutation in ste20 Rictor homolog, Ste20
FYPO localization affected by mutation in tor1 serine/threonine protein kinase Tor1
GO substrate of sst2 human AMSH/STAMBP protein homolog, ubiquitin specific-protease
GO substrate of ubp15 ubiquitin C-terminal hydrolase Ubp15
GO substrate of ubp4 ubiquitin C-terminal hydrolase Ubp4
GO substrate of ubp5 ubiquitin C-terminal hydrolase Ubp5
GO substrate of ubp9 ubiquitin C-terminal hydrolase Ubp9
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons212160..214376
mRNA212160..214376
5' UTR212160..212312AF017180
CDS212313..213953
3' UTR213954..214376SPD179
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00083 Pfam IPR005828 Major facilitator, sugar transporter-like 11 467 16
TMhelix TMHMM 118 135 953
TMhelix TMHMM 331 353 953
TMhelix TMHMM 368 390 953
TMhelix TMHMM 180 199 953
TMhelix TMHMM 89 111 953
TMhelix TMHMM 429 451 953
TMhelix TMHMM 145 167 953
TMhelix TMHMM 304 326 953
TMhelix TMHMM 7 29 953
TMhelix TMHMM 403 425 953
PS50850 Prosite Profiles IPR020846 Major facilitator superfamily domain 9 456 55
PS00216 Prosite Patterns IPR005829 Sugar transporter, conserved site 318 335 15
PTHR23500:SF131 HMMPANTHER 5 523 8
PTHR23500 HMMPANTHER 5 523 10
1.20.1250.20 Gene3D Major facilitator superfamily domain 263 457 72
1.20.1250.20 Gene3D Major facilitator superfamily domain 50 204 72
SSF103473 SuperFamily IPR020846 Major facilitator superfamily domain 8 465 76
SignalP-noTM signalp 1 21 207
PR00171 PRINTS IPR003663 Sugar/inositol transporter 369 390 10
PR00171 PRINTS IPR003663 Sugar/inositol transporter 392 404 10
PR00171 PRINTS IPR003663 Sugar/inositol transporter 114 133 10
PR00171 PRINTS IPR003663 Sugar/inositol transporter 17 27 10
PR00171 PRINTS IPR003663 Sugar/inositol transporter 276 286 10
TIGR00879 TIGRFAM IPR003663 Sugar/inositol transporter 7 463 10

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.48 Da
Charge -3.00
Codon Adaptation Index 0.71
Isoelectric point 5.90
Molecular weight 60.32 kDa
Number of residues 546
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1677
present during mitotic M phaseS537
present during mitotic M phaseS536
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S537 PMID:21712547
present during mitotic M phase experimental evidence S536 PMID:21712547
O-phospho-L-threonineT485 701
Annotation ExtensionEvidenceResidueReference
experimental evidence T485 PMID:24763107
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelpresent during GO:0072690Western blot evidencePMID:25411338
increased during GO:0042149Western blot evidencePMID:25411338
RNA levelincreased during GO:0042149quantitative PCRPMID:25411338
increased during GO:0071456expression microarray evidencePMID:22235339

Quantitative Gene Expression

View graphical display of gene expression data for ght5 (SPCC1235.14)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
96862during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
104327during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
95738during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
102458during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
102412during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
59226.53during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
10138.36during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
28during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Genome Organisation
DescriptionQualifierReferenceCount
tandem duplication30
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4606
conserved in eukaryotes4516
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPCC1235.14 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bypaa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
External References
Database Identifier Description
NBRP SPCC1235.14 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1235.14 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1235.14 BioGRID Interaction Datasets
Expression Viewer SPCC1235.14 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1235.14 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1235.14 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1235.14 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1235.14 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1235.14 Transcriptome Viewer (Bähler Lab)
GEO SPCC1235.14 GEO profiles
PInt SPCC1235.14 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC1235.14 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC1235.14 Fission yeast phenotypic data & analysis
Cyclebase SPCC1235.14.1 Cell Cycle Data
SPD / RIKEN34/34E03Orfeome Localization Data
UniProtKB/SwissProtP78831High-affinity glucose transporter ght5
ModBaseP78831Database of comparative protein structure models
STRINGP78831Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587740hexose transporter Ght5
RefSeq mRNANM_001022735972h- hexose transporter Ght5 (ght5), mRNA
European Nucleotide ArchiveAF051141ENA EMBL mapping
European Nucleotide ArchiveD89179ENA EMBL mapping
European Nucleotide ArchiveCAA21118.1ENA Protein Mapping
UniParcUPI000012B422UniProt Archive

Literature for ght5

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016