chk1 (SPCC1259.13)

Gene Standard Namechk1 ChromosomeIII
Systematic IDSPCC1259.13 Gene Start1059799
Synonymsrad27 Gene End1064443
ProductChk1 protein kinase Gene Length4645
Feature Typeprotein coding CDS Start1060020
Name Description CDS End1061775
Characterisation Statuspublished CDS Length1756

Ensembl Gene Location
  1. gyp2
  2. SPCC1259.12c
  3. SPNCRNA.1171
  4. ubc11
  5. chk1
  6. meu27
  7. SPCTRNAALA.12
  8. SPCTRNAVAL.12
  9. SPNCRNA.480
  10. SPCTRNASER.09
  11. SPCTRNAARG.10
  12. SPCTRNAASP.05
  13. SPCTRNAARG.11
  14. SPNCRNA.1172
  15. SPNCRNA.481
  16. SPCTRNALEU.11
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000085sensitive to camptothecinCell growth assaychk1delta (deletion)deletionPECO:0000005PMID:21971174
FYPO:0000088sensitive to hydroxyureaCell growth assaychk1delta (deletion)deletionPECO:0000005PMID:21971174
FYPO:0000089sensitive to methyl methanesulfonateCell growth assaychk1delta (deletion)deletionPECO:0000005PMID:21971174
FYPO:0002060viable vegetative cell populationMicroscopychk1delta (deletion)deletionPMID:20473289
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0004674protein serine/threonine kinase activityIDAPMID:10825192
has_direct_input cdc25IDAPMID:9278510
GO:0005515protein bindingIPIcdc25PMID:9278510
IPIsum3PMID:11711540
IPIcrb2PMID:9407031
IPIrad4PMID:9407031
IPIrad3PMID:10512862
GO:0005524ATP bindingISMPFAM:PF00069GO_REF:0000001
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0000077DNA damage checkpointIGIrad1PMID:8497322
GO:0006468protein phosphorylationIDAPMID:9278510
GO:0007095mitotic G2 DNA damage checkpointIMPPMID:9034337
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0005634nucleusIDAPMID:16823372
GO:0005654nucleoplasmTASPMID:9034337
GO:0005737cytoplasmIDAPMID:16823372
GO:0005829cytosolIDAPMID:16823372
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
110597991060087
210601331060374
310604211060849
410608881061095
510611421061331
610613771061673
710617191062218
810622721064443

References

Region Start End Reference
three_prime_UTR10622721064443PMID:21511999
three_prime_UTR10622721064443PMID:21511999
three_prime_UTR10617761062218PMID:21511999
five_prime_UTR10597991060019PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF00069 IPR000719 Pfam Protein kinase, catalytic domain 10 272 109
SM00220 IPR002290 SMART Serine/threonine- / dual specificity protein kinase, catalytic domain 10 272 74
PS50011 IPR000719 Prosite Profiles Protein kinase, catalytic domain 10 272 110
PS00107 IPR017441 Prosite Patterns Protein kinase, ATP binding site 16 38 73
PS00108 IPR008271 Prosite Patterns Serine/threonine-protein kinase, active site 133 145 97
G3DSA:3.30.200.20 Gene3D 10 123 108
G3DSA:1.10.510.10 Gene3D 124 274 111
SSF56112 IPR011009 SuperFamily 10 308 127
PTHR24347:SF57 hmmpanther 10 303 1
PTHR24347 IPR020636 hmmpanther Calcium/calmodulin-dependent/calcium-dependent protein kinase 10 303 6

View domain organization at Pfam

Protein Modifications (PSI-MOD)

Term IDTerm NameEvidenceResidueReference
MOD:00696phosphorylated residueresidueS345

Sequence Ontology

Term IDTerm NameReference
SO:0001528nuclear_localization_signal

Protein Properties

Ave. residue weight 113.92 Da
Charge 17.50
Isoelectric point 9.16
Molecular weight 56.51 kDa
Number of residues 496
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
deletion mutant expression profiling PMID:14668484
protein levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127		population_widePMID:23101633
experimental evidencesingle-celled organism vegetative growth phasePECO:0000005,
PECO:0000014		population_widePMID:23101633
RNA levelexperimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide1.1PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide0.5PMID:23101633
Species Distribution
DescriptionQualifierReference
predominantly single copy (one to one)
conserved in fungi
conserved in eukaryotes
conserved in metazoa
conserved in vertebrates
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
csn1COP9/signalosome complex subunit Csn1 Synthetic Growth DefectPMID:18931302
pol1DNA polymerase alpha catalytic subunit Phenotypic SuppressionPMID:14739927
rad2FEN-1 endonuclease Rad2 Phenotypic EnhancementPMID:23211746
Phenotypic Suppression
Dosage Rescue
ssb3DNA replication factor A subunit Ssb3 Synthetic Growth DefectPMID:20885790
rad17RFC related checkpoint protein Rad17 Synthetic RescuePMID:16816416
cdc24DNA replication protein Cdc24 Phenotypic EnhancementPMID:18667534
cdc45DNA replication pre-initiation complex subunit Cdc45 Synthetic Growth DefectPMID:16849602
Synthetic Growth DefectPMID:15338237
mus81Holliday junction resolvase subunit Mus81 Phenotypic EnhancementPMID:11073977
hst4Sir2 family histone deacetylase Hst4 Synthetic LethalityPMID:18344406
mus7DNA repair protein Mus7/Mms22 Phenotypic SuppressionPMID:17660542
cdc27DNA polymerase delta subunit Cdc27 Phenotypic EnhancementPMID:18667534
Synthetic LethalityPMID:23211746
Synthetic Growth DefectPMID:16738311
cdc20DNA polymerase epsilon catalytic subunit Pol2 Phenotypic EnhancementPMID:18667534
Phenotypic SuppressionPMID:14739927
Synthetic LethalityPMID:11416129
Synthetic Growth DefectPMID:10683155
Phenotypic Enhancement
pim1Ran GDP/GTP exchange factor Synthetic Growth DefectPMID:22927644
mcb1MCM binding protein homolog Mcb1 (predicted) Phenotypic EnhancementPMID:22036784
Synthetic Growth DefectPMID:23322785
Synthetic RescuePMID:21813639
Phenotypic Suppression
hta1histone H2A alpha Phenotypic EnhancementPMID:15226425
srw1CDK inhibitor Srw1 Dosage RescuePMID:9398669
rhp14XP-A family homolog Rhp14 Synthetic Growth DefectPMID:18931302
pof3F-box protein Pof3 Synthetic LethalityPMID:11809834
wee1M phase inhibitor protein kinase Wee1 Phenotypic EnhancementPMID:10637286
Synthetic LethalityPMID:11270571
Synthetic LethalityPMID:8497322
Synthetic RescuePMID:16816416
bdf1Swr1 complex bromodomain subunit Brf1 Synthetic Growth DefectPMID:22095079
Synthetic Rescue
mcm7MCM complex subunit Mcm7 Synthetic LethalityPMID:11606526
mrc1mediator of replication checkpoint 1 Synthetic Growth DefectPMID:11715017
rad4BRCT domain protein Rad4 Synthetic Growth DefectPMID:16723501
skp1SCF ubiquitin ligase complex subunit Skp1 Synthetic RescuePMID:15147268
brc1BRCT domain protein Brc1 Synthetic Growth DefectPMID:22366461
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic SuppressionPMID:10637286
Dosage RescuePMID:11711540
Dosage RescuePMID:8497322
pcn1PCNA Phenotypic EnhancementPMID:9928931
Synthetic Growth DefectPMID:9563836
cdr1NIM1 family serine/threonine protein kinase Cdr1/Nim1 Dosage Growth DefectPMID:16816416
Synthetic Rescue
rad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Phenotypic SuppressionPMID:15173168
Synthetic Growth DefectPMID:9154809
cdc17ATP-dependent DNA ligase Cdc17 Synthetic Growth DefectPMID:11270571
Synthetic Growth DefectPMID:12415000
Synthetic Lethality
Synthetic LethalityPMID:18183307
Synthetic LethalityPMID:15585577
Dosage Growth DefectPMID:15082762
ark1aurora-B kinase Ark1 Synthetic LethalityPMID:21712547
rad57RecA family ATPase Rad57/Rhp57 Synthetic Growth DefectPMID:18931302
tlg2SNARE Tlg2 (predicted) Synthetic Growth DefectPMID:18931302
ctf18RFC-like complex subunit Ctf18 Phenotypic EnhancementPMID:18045993
hrq1RecQ type DNA helicase Hrq1 (predicted) Phenotypic SuppressionPMID:22064477
Synthetic Lethality
Synthetic Growth Defect
ast1asteroid homolog, XP-G family protein Dosage RescuePMID:23211746
hus5SUMO conjugating enzyme Hus5 Phenotypic EnhancementPMID:7768995
tel2Tel2/Rad-5/Clk-2 family protein Tel2 Synthetic Growth DefectPMID:17189249
swi3replication fork protection complex subunit Swi3 Synthetic LethalityPMID:15367656
Synthetic Growth DefectPMID:18931302
Synthetic Growth DefectPMID:15767681
Phenotypic Enhancement
rid1GTPase binding protein Rid1 (predicted) Synthetic Growth DefectPMID:20799962
orc1origin recognition complex subunit Orc1 Phenotypic EnhancementPMID:18667534
Synthetic Growth DefectPMID:16849602
Phenotypic EnhancementPMID:12186947
Synthetic Growth DefectPMID:21712547
cdc22ribonucleoside reductase large subunit Cdc22 Phenotypic EnhancementPMID:10683155
spp2DNA primase large subunit Spp2 Synthetic LethalityPMID:11027257
top2DNA topoisomerase II Synthetic RescuePMID:21767457
Synthetic Lethality
Phenotypic Enhancement
Phenotypic Suppression
ams2cell cycle regulated GATA-type transcription factor Ams2 Phenotypic SuppressionPMID:23195958
mcm2MCM complex subunit Mcm2 Synthetic Growth DefectPMID:9914167
psf2GINS complex subunit Psf2 Synthetic Growth DefectPMID:15466421
rhp18Rad18 homolog Rhp18 Synthetic Growth DefectPMID:11523791
cut2securin, sister chromatid separation inhibitor Synthetic Growth DefectPMID:15329725
dfp1Hsk1-Dfp1 kinase complex regulatory subunit Dfp1 Phenotypic EnhancementPMID:10409743
rec12endonuclease Rec12 Synthetic RescuePMID:15689488
cut14condensin complex subunit Cut14 Synthetic Growth DefectPMID:22645654
top1DNA topoisomerase I Synthetic RescuePMID:10407262
rad54DNA-dependent ATPase Rad54/Rhp54 Synthetic Growth DefectPMID:8834792
rad2414-3-3 protein Rad24 Synthetic RescuePMID:16816416
nse6Smc5-6 complex non-SMC subunit Nse6 Synthetic Growth DefectPMID:16478984
tor1phosphatidylinositol kinase Tor1 Synthetic Growth DefectPMID:19546237
hta2histone H2A beta Phenotypic EnhancementPMID:15226425
cia1histone chaperone Cia1 Synthetic LethalityPMID:22291963
Phenotypic Suppression
rad31SUMO E1-like activator enzyme Rad31 Phenotypic EnhancementPMID:9092625
nrc1bromodomain protein (predicted) Synthetic RescuePMID:22095079
Synthetic Growth Defect
cdc25M phase inducer phosphatase Cdc25 Phenotypic EnhancementPMID:10637286
Phenotypic Suppression
Synthetic RescuePMID:8497322
Dosage RescuePMID:9832516
Phenotypic SuppressionPMID:22682874
Phenotypic Enhancement
rad1checkpoint clamp complex protein Rad1 Synthetic RescuePMID:8497322
Dosage RescuePMID:10430579
mcm4MCM complex subunit Mcm4/Cdc21 Phenotypic EnhancementPMID:18667534
Phenotypic EnhancementPMID:18753627
Phenotypic EnhancementPMID:10683155
orc2origin recognition complex subunit Orc2 Phenotypic EnhancementPMID:18667534
tra1SAGA complex phosphatidylinositol pseudokinase Tra1 Synthetic RescuePMID:20194963
crb2DNA repair protein Rad9 homolog, Rhp9 Synthetic RescuePMID:15229228
Dosage RescuePMID:9407031
Phenotypic SuppressionPMID:12081644
Synthetic RescuePMID:16816416
cds1replication checkpoint kinase Cds1 Synthetic Growth DefectPMID:17189249
Synthetic LethalityPMID:15367656
Phenotypic SuppressionPMID:15173168
Phenotypic EnhancementPMID:15498101
Synthetic Growth DefectPMID:11715017
Phenotypic EnhancementPMID:16354704
Phenotypic EnhancementPMID:9774107
Dosage Rescue
Phenotypic EnhancementPMID:12081644
Synthetic Growth DefectPMID:14560029
Synthetic Growth DefectPMID:12808110
Synthetic Growth DefectPMID:16849602
Phenotypic EnhancementPMID:17151242
Phenotypic EnhancementPMID:19819763
Synthetic RescuePMID:9870697
Synthetic Growth DefectPMID:15155581
Phenotypic EnhancementPMID:11073977
Phenotypic EnhancementPMID:9832516
rad3ATR checkpoint kinase Rad3 Dosage RescuePMID:9857181
Phenotypic SuppressionPMID:21098122
Synthetic RescuePMID:16816416
cdc1DNA polymerase delta small subunit Cdc1 Phenotypic EnhancementPMID:10683155
Dosage RescuePMID:10757807
Dosage RescuePMID:23211746
rhp41DNA repair protein Rhp41 Synthetic Growth DefectPMID:18931302
spp1DNA primase catalytic subunit Spp1 Phenotypic EnhancementPMID:11160827
msc1multi-copy suppressor of Chk1 Dosage RescuePMID:17456468
Dosage RescuePMID:15082762
rad51RecA family recombinase Rad51/Rhp51 Dosage RescuePMID:12839619
Synthetic Growth Defect
ddb1damaged DNA binding protein Ddb1 Synthetic LethalityPMID:15805471
Synthetic Growth DefectPMID:18931302
swi1replication fork protection complex subunit Swi1 Synthetic Growth DefectPMID:14560029
Phenotypic EnhancementPMID:15767681
Synthetic Growth Defect
sap1switch-activating protein Sap1 Synthetic Growth DefectPMID:17151242
taz1human TRF ortholog Taz1 Phenotypic SuppressionPMID:12196391
Synthetic Growth DefectPMID:12620220
pku70Ku domain protein Pku70 Phenotypic SuppressionPMID:12196391
hsk1Dbf4(Dfp1)-dependent protein kinase Hsk1 Synthetic Growth DefectPMID:16263721
Synthetic LethalityPMID:11027263
rqh1RecQ type DNA helicase Rqh1 Phenotypic EnhancementPMID:9372918
Phenotypic EnhancementPMID:15226425
Phenotypic SuppressionPMID:21098121
cdc6DNA polymerase delta catalytic subunit Cdc6 Phenotypic EnhancementPMID:18667534
Phenotypic SuppressionPMID:14739927
Phenotypic EnhancementPMID:9154809
Synthetic RescuePMID:9135148
cdc18MCM loader Phenotypic EnhancementPMID:18667534
exo1exonuclease I Exo1 Phenotypic EnhancementPMID:23211746
Phenotypic Suppression
pot1telomere end-binding protein Pot1 Phenotypic SuppressionPMID:21098121
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
rad4BRCT domain protein Rad4 Two-hybridPMID:16723501
Two-hybridPMID:9407031
dcp2mRNA decapping complex catalytic subunit Dcp2 Two-hybridPMID:17502373
SPCC613.03conserved fungal protein Two-hybridPMID:17502373
rfp1SUMO-targeted ubiquitin-protein ligase subunit Rfp1 Affinity Capture-WesternPMID:17502373
Two-hybrid
cdc25M phase inducer phosphatase Cdc25 Biochemical ActivityPMID:14739927
Biochemical ActivityPMID:9774107
Affinity Capture-WesternPMID:9278510
Biochemical ActivityPMID:10198041
Affinity Capture-WesternPMID:20639859
rad2514-3-3 protein Rad25 Affinity Capture-WesternPMID:10090724
Two-hybrid
crb3WD repeat protein Crb3 Two-hybridPMID:16723501
crb2DNA repair protein Rad9 homolog, Rhp9 Affinity Capture-WesternPMID:14739927
Affinity Capture-WesternPMID:22792081
Reconstituted Complex
Two-hybridPMID:15229228
Two-hybridPMID:9407031
Affinity Capture-WesternPMID:23422000
pcn1PCNA Two-hybridPMID:22308326
wee1M phase inhibitor protein kinase Wee1 Biochemical ActivityPMID:9034337
rad2414-3-3 protein Rad24 Affinity Capture-WesternPMID:12415000
Affinity Capture-WesternPMID:10090724
Two-hybrid
Two-hybridPMID:17502373
Affinity Capture-WesternPMID:15585577
Affinity Capture-WesternPMID:20639859
rad3ATR checkpoint kinase Rad3 Affinity Capture-WesternPMID:9857181
Biochemical Activity
Biochemical ActivityPMID:11553781
Affinity Capture-WesternPMID:10512862
sum3translation initiation RNA helicase Sum3 Affinity Capture-WesternPMID:11711540
Two-hybrid
Two-hybridPMID:17502373
cek1serine/threonine protein kinase Cek1 Two-hybridPMID:17502373
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External References
Database Identifier Description
NBRP SPCC1259.13 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1259.13 Retrieval of eukaryotic orthologs
BioGrid SPCC1259.13 BioGRID Interaction Datasets
Bähler Lab SPCC1259.13 Cell Cycle Expression Profile
Bähler Lab SPCC1259.13 Meiosis/Sporulation Expression Profies
Bähler Lab SPCC1259.13 Pheromone response/mating expression profiles
Bähler Lab SPCC1259.13 Environmental stress expression profiles
Bähler Lab SPCC1259.13 Bähler Lab Transcriptome Viewer
Cyclebase SPCC1259.13 Cell Cycle Data
PInt SPCC1259.13 Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPCC1259.13 Entrez Gene
WikiGene2539087Chk1 protein kinase
Uniprot_genenameCHK1
EntrezGene2539087Chk1 protein kinase
SPD / RIKEN45/45G09Orfeome Localization Data
UniProtKB/SwissProtP34208Serine/threonine-protein kinase chk1
ModBaseP34208Database of comparative protein structure models
Pfam Protein DomainsP34208Pfam Domain Arrangement
RefSeq PeptideNP_588070Chk1 protein kinase
RefSeq mRNANM_001023062972h- Chk1 protein kinase (chk1), mRNA
European Nucleotide ArchiveAAA79758ENA Protein Mapping
European Nucleotide ArchiveAAA80539ENA Protein Mapping
European Nucleotide ArchiveBAA20374ENA Protein Mapping
European Nucleotide ArchiveCAA22551ENA Protein Mapping
UniParcUPI0000127835UniProt Archive

Literature for chk1
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013