pku70 (SPCC126.02c)


Gene Standard Namepku70 Characterisation Statuspublished
Systematic IDSPCC126.02c Feature Typeprotein coding
Synonyms Name Description
ProductKu domain protein Pku70 Product Size607aa, 69.10 kDa
Genomic Location Chromosome III, 2119699-2117095 (2605nt); CDS:2119418-2117287 (2132nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding557
Annotation ExtensionEvidenceWith/FromReference
ATP-dependent DNA helicase activity40
Annotation ExtensionEvidenceWith/FromReference
damaged DNA binding19
Annotation ExtensionEvidenceWith/FromReference
telomeric DNA binding14
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
double-strand break repair via nonhomologous end joining9
Annotation ExtensionEvidenceWith/FromReference
negative regulation of telomere maintenance via recombination3
Annotation ExtensionEvidenceWith/FromReference
telomere maintenance45
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Ku70:Ku80 complex2
Annotation ExtensionEvidenceWith/FromReference
nuclear chromosome, telomeric region53
Annotation ExtensionEvidenceWith/FromReference
nuclear telomeric heterochromatin14
Annotation ExtensionEvidenceWith/FromReference
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
subtelomeric heterochromatin29
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased population viability in presence of persistent double-strand breakspku70Δ5
decreased spore germination frequencypku70Δ69
normal growth during cellular response to ionizing radiationpku70Δ25
normal growth during cellular response to UVpku70Δ79
normal growth on bleomycinpku70Δ7
normal growth on camptothecinpku70Δ43
normal growth on hydroxyureapku70Δ75
normal growth on methyl methanesulfonatepku70Δ58
normal growth on phleomycinpku70Δ3
normal vegetative cell population growthpku70Δ813
normal vegetative cell population growth ratepku70Δ80
sensitive to ionizing radiation during mitotic G1 phasepku70Δ2
viable vegetative cell populationpku70Δ3850

Cell Phenotype

Term NameGenotypesCount
abnormal telomere maintenancepku70Δ3
abolished double-strand break repair via nonhomologous end joiningpku70Δ1
altered double-strand break repair junction in presence of persistent double-strand breakspku70Δ5
decreased double-strand break repair during vegetative growthpku70Δ16
decreased double-strand break repair via nonhomologous end joiningpku70Δ3
decreased fidelity of double-strand break repair via nonhomologous end joiningpku70Δ2
decreased minichromosome loss during vegetative growthpku70Δ4
increased circular minichromosome loss during vegetative growthpku70Δ1
increased gross chromosomal rearrangementpku70Δ6
increased linear minichromosome loss during vegetative growthpku70Δ3
increased telomeric 3' overhang lengthpku70Δ2
normal cellular reactive oxygen species level during vegetative growthpku70Δ16
normal chromatin silencing at telomerepku70Δ16
normal frequency of double-strand break repair via homologous recombinationpku70Δ1
normal protein localization to telomere during vegetative growth14
affecting trt1pku70Δ
normal protein phosphorylation during cellular response to bleomycin13
affecting ctp1pku70Δ
normal telomere lengthpku70Δ35
shortened telomerespku70Δ44
spores sensitive to ionizing radiationpku70Δ7
stable shortened telomerespku70Δ5
viable vegetative cell with normal cell morphologypku70Δ3102

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased spore germination frequencypku70Δ, taz1Δ
normal growth during cellular response to UVrad50Δ, pku70Δ
normal growth on methyl methanesulfonateexo1Δ, pku70Δ
pku70Δ, mre11Δ, ast1Δ
sensitive to camptothecinbrc1Δ, pku70Δ
sensitive to hydroxyureabrc1Δ, pku70Δ
rad50Δ, pku70Δ
sensitive to ionizing radiation during mitotic G1 phasehta1-S128A (S128A), hta2-S129A (S129A), pku70Δ
sensitive to ionizing radiation during vegetative growthrad50Δ, pku70Δ
rad51Δ, pku70Δ, hta1-S129A (S129A), hta2-S128A (S128A)
pku70Δ, rad54Δ
pku70Δ, rad11-D223Y (D223Y)
rad51Δ, pku70Δ
exo1Δ, rad50Δ, pku70Δ
sensitive to methyl methanesulfonaterad51Δ, pku70Δ
rad50Δ, pku70Δ
exo1Δ, rad50Δ, pku70Δ
mre11Δ, exo1Δ, pku70Δ
mre11Δ, pku70Δ
brc1Δ, pku70Δ
sensitive to UV during vegetative growthbrc1Δ, pku70Δ

Cell Phenotype

Term NameGenotypes
abnormal telomere morphologytrt1-D743A (D743A), pku70Δ, taz1Δ
trt1Δ, pku70Δ, taz1Δ
trt1-D590A (D590A), pku70Δ, taz1Δ
abolished break-induced loss of heterozygosity via chromosomal translocationrad55Δ, pku70Δ
abolished double-strand break repair via homologous recombinationrad51Δ, pku70Δ
abolished protein localization to telomere during vegetative growth
affects localization of trt1pku70Δ, taz1Δ
affects localization of trt1pku70Δ, trt1-D590A (D590A), taz1Δ
affects localization of trt1pku70Δ, trt1-D743A (D743A), taz1Δ
circularized chromosometrt1Δ, taz1Δ, tel1Δ, pku70Δ
trt1Δ, pku70Δ
est1Δ, pku70Δ, taz1Δ
rad3Δ, tel1Δ, pku70Δ
decreased double-strand break repair via homologous recombinationrad55Δ, pku70Δ
decreased gene conversion during vegetative growthrad55Δ, pku70Δ
elongated telomerespku70Δ, taz1Δ
increased break-induced loss of heterozygosityrad55Δ, pku70Δ
increased minichromosome loss during vegetative growthrad55Δ, pku70Δ
increased telomeric 3' overhang lengthexo1Δ, pku70Δ, rad50Δ, taz1Δ
taz1Δ, rad50Δ, pku70Δ
exo1Δ, pku70Δ, mre11Δ, taz1Δ
taz1Δ, mre11Δ, pku70Δ
shortened telomeresmre11Δ, pku70Δ
trt1+, pku70Δ
rad17Δ, pku70Δ
hus1Δ, tel1Δ, pku70Δ
rad3Δ, pku70Δ
rad26Δ, pku70Δ
tel1Δ, rad1Δ, pku70Δ
chk1Δ, pku70Δ
cds1Δ, pku70Δ
rad9Δ, pku70Δ
rad11-D223Y (D223Y), pku70Δ
crb2Δ, pku70Δ
chk1Δ, cds1Δ, pku70Δ
tel1Δ, pku70Δ
rad1Δ, pku70Δ
rad9Δ, tel1Δ, pku70Δ
hus1Δ, pku70Δ
rad17Δ, tel1Δ, pku70Δ
telomere assembly at double-strand break siterad55Δ, pku70Δ
telomeric 3' overhang absentpku70Δ, dna2-C2 (T3236C), taz1Δ
Target Of
OntologyRelationshipGeneProduct
FYPO localization affected by mutation in brc1 BRCT domain protein Brc1
FYPO localization affected by mutation in ctp1 CtIP-related endonuclease
FYPO localization affected by mutation in taz1 human TRF ortholog Taz1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2119699..2119609, 2119559..2119386, 2119320..2119138, 2119066..2118970, 2118895..2117706, 2117660..2117451, 2117397..2117095
Intron2119608..2119560, 2119385..2119321, 2119137..2119067, 2118969..2118896, 2117705..2117661, 2117450..2117398
mRNA2119699..2117095
5' UTR2119699..2119609, 2119559..2119419PMID:21511999
CDS2119418..2119386, 2119320..2119138, 2119066..2118970, 2118895..2117706, 2117660..2117451, 2117397..2117287
3' UTR2117286..2117095PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03731 Pfam IPR005161 Ku70/Ku80, N-terminal alpha/beta 19 236 1
PF03730 Pfam IPR005160 Ku70/Ku80 C-terminal arm 457 543 1
PF02037 Pfam IPR003034 SAP domain 571 603 3
PF02735 Pfam IPR006164 Ku70/Ku80 beta-barrel domain 244 432 2
SM00559 SMART IPR006164 Ku70/Ku80 beta-barrel domain 290 436 2
SM00513 SMART IPR003034 SAP domain 570 604 5
PS50800 Prosite Profiles IPR003034 SAP domain 570 604 6
PTHR12604:SF2 HMMPANTHER IPR006165 Ku70 2 606 1
PTHR12604 HMMPANTHER 2 606 2
1.10.1600.10 Gene3D IPR005160 Ku70/Ku80 C-terminal arm 422 514 2
3.40.50.410 Gene3D IPR002035 von Willebrand factor, type A 19 207 9
2.40.290.10 Gene3D IPR016194 SPOC-like, C-terminal domain 294 420 2
1.10.720.30 Gene3D IPR003034 SAP domain 554 605 4
SSF68906 SuperFamily 558 605 4
SSF53300 SuperFamily IPR002035 von Willebrand factor, type A 16 224 9
SSF100939 SuperFamily IPR016194 SPOC-like, C-terminal domain 234 514 2
PIRSF003033 PIRSF IPR006165 Ku70 1 606 1
Coil ncoils Predicted coiled-coil protein (DUF2205) 597 607 1055
Coil ncoils Predicted coiled-coil protein (DUF2205) 514 534 1055
TIGR00578 TIGRFAM IPR006165 Ku70 13 605 1

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000657SAP domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000657

Protein Properties

Ave. residue weight 113.84 Da
Charge 5.50
Codon Adaptation Index 0.37
Isoelectric point 7.59
Molecular weight 69.10 kDa
Number of residues 607
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for pku70 (SPCC126.02c)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.44during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Subunit Composition
DescriptionQualifierReferenceCount
heteromeric(2) PMID:1242424443
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3093
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPCC126.02c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withpku80Ku domain protein Pku80 Two-hybridPMID:12424244
modified bypli1SUMO E3 ligase Pli1 Biochemical ActivityPMID:18031226
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
affinity captured bycdc2cyclin-dependent protein kinase Cdk1/Cdc2 Affinity Capture-WesternPMID:24861625
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPCC126.02c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
rescueschk1Chk1 protein kinase Phenotypic SuppressionPMID:12196391
rescuesmre11Mre11 nuclease Phenotypic SuppressionPMID:12196391
rescuescrb2DNA repair protein Rad9 homolog Crb2 Phenotypic SuppressionPMID:12196391
rescuescds1replication checkpoint kinase Cds1 Phenotypic SuppressionPMID:12196391
rescuestel1ATM checkpoint kinase Phenotypic SuppressionPMID:12196391
rescuestaz1human TRF ortholog Taz1 Phenotypic SuppressionPMID:23335786
rescued byrad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Phenotypic SuppressionPMID:12196391
rescued byrad17RFC related checkpoint protein Rad17 Phenotypic SuppressionPMID:12196391
rescued byexo1exonuclease I Exo1 Phenotypic SuppressionPMID:21123655
rescued byhus1checkpoint clamp complex protein Hus1 Phenotypic SuppressionPMID:12196391
rescued bytaz1human TRF ortholog Taz1 Phenotypic SuppressionPMID:12196391
rescued byrad1checkpoint clamp complex protein Rad1 Phenotypic SuppressionPMID:12196391
rescued byspd1ribonucleotide reductase (RNR) inhibitor Phenotypic SuppressionPMID:21123655
rescued byrad9checkpoint clamp complex protein Rad9 Phenotypic SuppressionPMID:12196391
rescued byrad3ATR checkpoint kinase Rad3 Phenotypic SuppressionPMID:12196391
synthetic growth defect withhta1histone H2A alpha Synthetic Growth DefectPMID:15226425
synthetic growth defect withtrt1telomerase reverse transcriptase 1 protein Trt1 Synthetic Growth DefectPMID:11029034
synthetic growth defect withpnk1DNA kinase/phosphatase Pnk1 Synthetic Growth DefectPMID:22748672
synthetic growth defect withhta2histone H2A beta Synthetic Growth DefectPMID:15226425
synthetically rescueshob1BAR adaptor protein Hob1 Synthetic RescuePMID:17671430
synthetically rescuestaz1human TRF ortholog Taz1 Synthetic RescuePMID:11172711
synthetically rescuesctp1CtIP-related endonuclease Synthetic RescuePMID:21931565
synthetically rescuesrad50DNA repair protein Rad50 Synthetic RescuePMID:12861005
synthetically rescuesmre11Mre11 nuclease Synthetic RescuePMID:12861005
phenotype enhanced bypli1SUMO E3 ligase Pli1 Phenotypic EnhancementPMID:17209013
enhances phenotype ofddb1damaged DNA binding protein Ddb1 Phenotypic EnhancementPMID:21123655
synthetic lethal withcut14condensin complex SMC subunit Smc2 Synthetic LethalityPMID:22645654
External References
Database Identifier Description
NBRP SPCC126.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC126.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC126.02c BioGRID Interaction Datasets
Expression Viewer SPCC126.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC126.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC126.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC126.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC126.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC126.02c Transcriptome Viewer (Bähler Lab)
GEO SPCC126.02c GEO profiles
PInt SPCC126.02c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC126.02c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC126.02c Fission yeast phenotypic data & analysis
Cyclebase SPCC126.02c.1 Cell Cycle Data
SPD / RIKEN37/37D11Orfeome Localization Data
UniProtKB/SwissProtO94395ATP-dependent DNA helicase II subunit 1
ModBaseO94395Database of comparative protein structure models
STRINGO94395Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588445Ku domain protein Pku70
RefSeq mRNANM_001023436972h- Ku domain protein Pku70 (pku70), mRNA
European Nucleotide ArchiveCAA22471.1ENA Protein Mapping
UniParcUPI000006A1C5UniProt Archive

Literature for pku70

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016