pku70 (SPCC126.02c)


Gene Standard Namepku70 Characterisation Statuspublished
Systematic IDSPCC126.02c Feature Typeprotein coding
Synonyms Name Description
ProductKu domain protein Pku70 Product Size607aa, 69.10 kDa
Genomic Location Chromosome III, 2119699-2117095 (2605nt); CDS:2119418-2117287 (2132nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037559
GO:0004003ATP-dependent DNA helicase activityIEAIPR005160GO_REF:000000243
GO:0003684damaged DNA bindingIEAIPR006165GO_REF:000000219
GO:0046982protein heterodimerization activityIPIpku80PMID:1242424461
GO:0042162telomeric DNA bindingIDAPMID:1242424413
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006310DNA recombinationIEAUniProtKB-KW:KW-0233GO_REF:0000037109
GO:0006303double-strand break repair via nonhomologous end joiningIMPPMID:110290347
GO:0000723telomere maintenance50
requiredIMPPMID:11029034
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0043564Ku70:Ku80 complexIEAIPR006165GO_REF:00000022
GO:0000781chromosome, telomeric regionIDAPMID:1242424455
GO:0005634nucleusIDAPMID:168233722730
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopypku70ΔNullPMID:204732893755
Microscopypku70ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopypku70ΔNullPECO:0000005, PECO:0000137PMID:236978063078
penetrance FYPO_EXT:0000001
Target Of
RelationshipGeneProductReference
localized by mre11 Mre11 nuclease PMID:17936710
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
121196992119609
221195592119386
321193202119138
421190662118970
521188952117706
621176602117451
721173972117095

UTRs

Region Start End Reference
three_prime_UTR21172862117095PMID:21511999
five_prime_UTR21196992119609PMID:21511999
five_prime_UTR21195592119419PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03730 Pfam IPR005160 Ku70/Ku80 C-terminal arm 456 545 1
PF02037 Pfam IPR003034 SAP domain 571 603 5
PF03731 Pfam IPR005161 Ku70/Ku80, N-terminal alpha/beta 19 236 2
PF02735 Pfam IPR006164 Ku70/Ku80 beta-barrel domain 242 437 2
SM00327 SMART IPR002035 von Willebrand factor, type A 17 223 4
SM00559 SMART IPR006164 Ku70/Ku80 beta-barrel domain 290 436 2
SM00513 SMART IPR003034 SAP domain 570 604 5
PS50800 Prosite Profiles IPR003034 SAP domain 570 604 6
PTHR12604:SF2 HMMPANTHER IPR006165 4 606 1
PTHR12604 HMMPANTHER 4 606 2
G3DSA:3.40.50.410 Gene3D IPR002035 19 207 9
G3DSA:1.10.720.30 Gene3D IPR003034 554 605 4
G3DSA:2.40.290.10 Gene3D IPR016194 294 420 2
G3DSA:1.10.1600.10 Gene3D IPR005160 422 514 2
SSF100939 SuperFamily IPR016194 234 514 2
SSF68906 SuperFamily 558 605 4
SSF53300 SuperFamily 16 224 9
PIRSF003033 PIRSF IPR006165 Ku70 1 606 1
Coil ncoils Rabaptin coiled-coil domain 514 535 975
Low complexity (SEG) seg 299 310
Low complexity (SEG) seg 427 443
TIGR00578 tigrfam IPR006165 Ku70 13 605 1

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000657SAP domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000657

Protein Properties

Ave. residue weight 113.84 Da
Charge 5.50
Isoelectric point 7.59
Molecular weight 69.10 kDa
Number of residues 607
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
NDduring GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.44during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
rad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Phenotypic SuppressionPMID:12196391
taz1human TRF ortholog Taz1 Phenotypic SuppressionPMID:23335786
Phenotypic SuppressionPMID:12196391
Synthetic RescuePMID:11172711
pnk1DNA kinase/phosphatase Pnk1 Synthetic Growth DefectPMID:22748672
mre11Mre11 nuclease Synthetic RescuePMID:12861005
ctp1CtIP-related endonuclease Synthetic RescuePMID:21931565
rad17RFC related checkpoint protein Rad17 Phenotypic SuppressionPMID:12196391
hob1BAR adaptor protein Hob1 Synthetic RescuePMID:17671430
spd1ribonucleotide reductase (RNR) inhibitor Phenotypic SuppressionPMID:21123655
cut14condensin complex subunit Cut14 Synthetic LethalityPMID:22645654
hta1histone H2A alpha Synthetic Growth DefectPMID:15226425
hta2histone H2A beta Synthetic Growth DefectPMID:15226425
cds1replication checkpoint kinase Cds1 Phenotypic SuppressionPMID:12196391
rad50DNA repair protein Rad50 Synthetic RescuePMID:12861005
chk1Chk1 protein kinase Phenotypic SuppressionPMID:12196391
rad3ATR checkpoint kinase Rad3 Phenotypic SuppressionPMID:12196391
pli1SUMO E3 ligase Pli1 Phenotypic EnhancementPMID:17209013
trt1telomerase reverse transcriptase 1 protein Trt1 Synthetic Growth DefectPMID:11029034
exo1exonuclease I Exo1 Phenotypic EnhancementPMID:21123655
Phenotypic Suppression
crb2DNA repair protein Rad9 homolog, Rhp9 Phenotypic SuppressionPMID:12196391
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
pli1SUMO E3 ligase Pli1 Biochemical ActivityPMID:18031226
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
pku80Ku domain protein Pku80 Two-hybridPMID:12424244
External References
Database Identifier Description
NBRP SPCC126.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC126.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC126.02c BioGRID Interaction Datasets
Expression Viewer SPCC126.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC126.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC126.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC126.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC126.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC126.02c Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC126.02c Cell Cycle Data
GEO SPCC126.02c GEO profiles
PInt SPCC126.02c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2539090Ku domain protein Pku70
EntrezGene2539090Ku domain protein Pku70
SPD / RIKEN37/37D11Orfeome Localization Data
UniProtKB/SwissProtO94395ATP-dependent DNA helicase II subunit 1
ModBaseO94395Database of comparative protein structure models
StringO94395Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588445Ku domain protein Pku70
RefSeq mRNANM_001023436972h- Ku domain protein Pku70 (pku70), mRNA
European Nucleotide ArchiveCAA22471ENA Protein Mapping
UniParcUPI000006A1C5UniProt Archive

Literature for pku70

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014