pku70 (SPCC126.02c)


Gene Standard Namepku70 Characterisation Statuspublished
Systematic IDSPCC126.02c Feature Typeprotein coding
Synonyms Name Description
ProductKu domain protein Pku70 Product Size607aa, 69.10 kDa
Genomic Location Chromosome III, 2119699-2117095 (2605nt); CDS:2119418-2117287 (2132nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
ATP-dependent DNA helicase activity42
Annotation ExtensionEvidenceWith/FromReference
damaged DNA binding18
Annotation ExtensionEvidenceWith/FromReference
telomeric DNA binding13
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
DNA recombination114
Annotation ExtensionEvidenceWith/FromReference
double-strand break repair via nonhomologous end joining6
Annotation ExtensionEvidenceWith/FromReference
telomere maintenance48
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Ku70:Ku80 complex2
Annotation ExtensionEvidenceWith/FromReference
nuclear chromosome, telomeric region56
Annotation ExtensionEvidenceWith/FromReference
nucleus2740
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0003183normal growth on phleomycinCell growth assaypku70ΔNullPMID:176714301
FYPO:0001420normal vegetative cell population growth rateCell growth assaypku70ΔNullPMID:1102903451
FYPO:0002060viable vegetative cell populationMicroscopypku70ΔNullPECO:0000005, PECO:0000137PMID:236978063759
Microscopypku70ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000122abnormal telomere maintenanceGel electrophoresis evidencepku70ΔNullPMID:110290343
FYPO:0003660decreased double-strand break repairOtherpku70ΔNullPMID:110290346
FYPO:0002239shortened telomeresGel electrophoresis evidencepku70ΔNullPMID:1767143033
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopypku70ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
GO localized by mre11 Mre11 nuclease PMID:17936710
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
121196992119609
221195592119386
321193202119138
421190662118970
521188952117706
621176602117451
721173972117095

UTRs

Region Coordinates Reference
five_prime_UTR2119699..2119609,2119559..2119419PMID:21511999
three_prime_UTR2117286..2117095PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02037 Pfam IPR003034 SAP domain 571 603 5
PF02735 Pfam IPR006164 Ku70/Ku80 beta-barrel domain 242 437 2
PF03730 Pfam IPR005160 Ku70/Ku80 C-terminal arm 456 545 1
PF03731 Pfam IPR005161 Ku70/Ku80, N-terminal alpha/beta 19 236 2
SM00559 SMART IPR006164 Ku70/Ku80 beta-barrel domain 290 436 2
SM00513 SMART IPR003034 SAP domain 570 604 5
PS50800 Prosite Profiles IPR003034 SAP domain 570 604 6
PTHR12604:SF2 HMMPANTHER IPR006165 Ku70 4 606 1
PTHR12604 HMMPANTHER 4 606 2
2.40.290.10 Gene3D IPR016194 SPOC like C-terminal domain 294 420 2
1.10.720.30 Gene3D IPR003034 SAP domain 554 605 4
3.40.50.410 Gene3D IPR002035 von Willebrand factor, type A 19 207 9
1.10.1600.10 Gene3D IPR005160 Ku70/Ku80 C-terminal arm 422 514 2
SSF68906 SuperFamily 558 605 4
SSF53300 SuperFamily von Willebrand factor, type A 16 224 9
SSF100939 SuperFamily IPR016194 SPOC like C-terminal domain 234 514 2
PIRSF003033 PIRSF IPR006165 Ku70 1 606 1
Coil ncoils Rabaptin coiled-coil domain 514 535 968
TIGR00578 tigrfam IPR006165 Ku70 13 605 1

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000657SAP domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000657

Protein Properties

Ave. residue weight 113.84 Da
Charge 5.50
Isoelectric point 7.59
Molecular weight 69.10 kDa
Number of residues 607
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.44during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Subunit Composition
DescriptionQualifierReferenceCount
heteromeric(2) PMID:1242424442
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
pli1SUMO E3 ligase Pli1 Biochemical ActivityPMID:18031226
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Affinity Capture-WesternPMID:24861625
pku80Ku domain protein Pku80 Two-hybridPMID:12424244
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
pnk1DNA kinase/phosphatase Pnk1 Synthetic Growth DefectPMID:22748672
taz1human TRF ortholog Taz1 Phenotypic SuppressionPMID:23335786
Phenotypic SuppressionPMID:12196391
Synthetic RescuePMID:11172711
hob1BAR adaptor protein Hob1 Synthetic RescuePMID:17671430
spd1ribonucleotide reductase (RNR) inhibitor Phenotypic SuppressionPMID:21123655
rad9checkpoint clamp complex protein Rad9 Phenotypic SuppressionPMID:12196391
ctp1CtIP-related endonuclease Synthetic RescuePMID:21931565
mre11Mre11 nuclease Synthetic RescuePMID:12861005
Phenotypic SuppressionPMID:12196391
ddb1damaged DNA binding protein Ddb1 Phenotypic EnhancementPMID:21123655
cut14condensin complex subunit Cut14 Synthetic LethalityPMID:22645654
hta2histone H2A beta Synthetic Growth DefectPMID:15226425
tel1ATM checkpoint kinase Phenotypic SuppressionPMID:12196391
rad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Phenotypic SuppressionPMID:12196391
hta1histone H2A alpha Synthetic Growth DefectPMID:15226425
rad3ATR checkpoint kinase Rad3 Phenotypic SuppressionPMID:12196391
pli1SUMO E3 ligase Pli1 Phenotypic EnhancementPMID:17209013
exo1exonuclease I Exo1 Phenotypic SuppressionPMID:21123655
crb2DNA repair protein Rad9 homolog, Rhp9 Phenotypic SuppressionPMID:12196391
rad1checkpoint clamp complex protein Rad1 Phenotypic SuppressionPMID:12196391
rad17RFC related checkpoint protein Rad17 Phenotypic SuppressionPMID:12196391
chk1Chk1 protein kinase Phenotypic SuppressionPMID:12196391
cds1replication checkpoint kinase Cds1 Phenotypic SuppressionPMID:12196391
rad50DNA repair protein Rad50 Synthetic RescuePMID:12861005
trt1telomerase reverse transcriptase 1 protein Trt1 Synthetic Growth DefectPMID:11029034
hus1checkpoint clamp complex protein Hus1 Phenotypic SuppressionPMID:12196391
External References
Database Identifier Description
NBRP SPCC126.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC126.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC126.02c BioGRID Interaction Datasets
Expression Viewer SPCC126.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC126.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC126.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC126.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC126.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC126.02c Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC126.02c Cell Cycle Data
GEO SPCC126.02c GEO profiles
PInt SPCC126.02c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC126.02c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC126.02c Fission yeast phenotypic data & analysis
SPD / RIKEN37/37D11Orfeome Localization Data
UniProtKB/SwissProtO94395ATP-dependent DNA helicase II subunit 1
ModBaseO94395Database of comparative protein structure models
STRINGO94395Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588445Ku domain protein Pku70
RefSeq mRNANM_001023436972h- Ku domain protein Pku70 (pku70), mRNA
European Nucleotide ArchiveCAA22471.1ENA Protein Mapping
UniParcUPI000006A1C5UniProt Archive

Literature for pku70

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014