SPCC126.12


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPCC126.12 Feature Typeprotein coding
Synonyms Name Description
ProductGTP cyclohydrolase I involved in 7,8-dihydroneopterin 3'-triphosphate biosynthesis (predicted) Product Size278aa, 29.85 kDa
Genomic Location Chromosome III, 2137595-2139203 (1609nt); CDS:2138121-2139127 (1007nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
GTP binding113
Annotation ExtensionEvidenceWith/FromReference
hydrolase activity837
Annotation ExtensionEvidenceWith/FromReference
metal ion binding745
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
cytoplasm4207
Annotation ExtensionEvidenceWith/FromReference
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
mitochondrion761
Annotation ExtensionEvidenceWith/FromReference
nucleus2695
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
sensitive to methyl methanesulfonateSPCC126.12ΔNull237
viable vegetative cell populationSPCC126.12ΔNull3795

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologySPCC126.12ΔNull3093
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2137595..2138334, 2138388..2138702, 2138755..2138925, 2138991..2139203
mRNA2137595..2139203
5' UTR2137595..2138120AU008770
CDS2138121..2138334, 2138388..2138702, 2138755..2138925, 2138991..2139127
3' UTR2139128..2139203PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01784 Pfam IPR002678 GTP cyclohydrolase 1 type 2/Nif3 10 256 1
PTHR13799:SF13 HMMPANTHER 1 278 1
PTHR13799 HMMPANTHER IPR002678 GTP cyclohydrolase 1 type 2/Nif3 1 278 1
3.40.1390.30 Gene3D 9 115 1
3.40.1390.30 Gene3D 116 235 1
SSF102705 SuperFamily IPR002678 GTP cyclohydrolase 1 type 2/Nif3 9 276 1
TIGR00486 tigrfam IPR002678 GTP cyclohydrolase 1 type 2/Nif3 9 274 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 107.38 Da
Charge 0.00
Isoelectric point 6.51
Molecular weight 29.85 kDa
Number of residues 278
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
35039during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
20900during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
18019during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
23645during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3958.45during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
23306during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2265.03during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.2during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in bacteria1000
conserved in metazoa3430
conserved in vertebrates3405
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Positive GeneticPMID:21504829
positive genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Positive GeneticPMID:22681890
positive genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Positive GeneticPMID:22681890
positive genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Positive GeneticPMID:22681890
positive genetic interaction withres1MBF transcription factor complex subunit Res1 Positive GeneticPMID:22681890
negative genetic interaction withSPCC16C4.106-phosphogluconolactonase (predicted) Negative GeneticPMID:22681890
positive genetic interaction withvph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPCC126.12 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC126.12 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC126.12 BioGRID Interaction Datasets
Expression Viewer SPCC126.12 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC126.12 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC126.12 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC126.12 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC126.12 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC126.12 Transcriptome Viewer (Bähler Lab)
GEO SPCC126.12 GEO profiles
PInt SPCC126.12 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC126.12 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC126.12 Fission yeast phenotypic data & analysis
Cyclebase SPCC126.12.1 Cell Cycle Data
SPD / RIKEN14/14H02Orfeome Localization Data
UniProtKB/SwissProtO94404Putative GTP cyclohydrolase 1 type 2
ModBaseO94404Database of comparative protein structure models
STRINGO94404Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588455NGG1 interacting factor 3 family protein
RefSeq mRNANM_001023446972h- NGG1 interacting factor 3 family protein (SPCC126.12), mRNA
European Nucleotide ArchiveCAA22481.1ENA Protein Mapping
MetaCycPWY-5663Tetrahydrobiopterin biosynthesis I
MetaCycPWY-5664Tetrahydrobiopterin biosynthesis II
MetaCycPWY-61476-hydroxymethyl-dihydropterin diphosphate biosynthesis I
MetaCycPWY-6703Preq0 biosynthesis
MetaCycPWY-6983Tetrahydrobiopterin biosynthesis III
MetaCycPWY-7442Drosopterin and aurodrosopterin biosynthesis
KEGG_Enzyme00790+3.5.4.16Folate biosynthesis
UniParcUPI000013B4B4UniProt Archive
UniPathwayUPA00848Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1

Literature for SPCC126.12

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015