prp18 (SPCC126.14)


Gene Standard Nameprp18 Characterisation Statusbiological_role_inferred
Systematic IDSPCC126.14 Feature Typeprotein coding
Synonyms Name Description
ProductU5 snRNP-associated protein Prp18 (predicted) Product Size343aa, 40.30 kDa
Genomic Location Chromosome III, 2140983-2143550 (2568nt); CDS:2141190-2142266 (1077nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
mRNA cis splicing, via spliceosome118
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nucleus2697
Annotation ExtensionEvidenceWith/FromReference
spliceosomal complex108
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationprp18ΔNull1428

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic cell cycleprp18ΔNull808
inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cellsprp18ΔNull87
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
121409832142146
221421922143550

UTRs

Region Coordinates Reference
five_prime_UTR2140983..2141189PMID:21511999
three_prime_UTR2142267..2143550PMID:21511999
mRNA2140983..2143550
exon2141190..2142146,2142192..2142266
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08799 Pfam IPR014906 Pre-mRNA processing factor 4 (PRP4)-like 129 158 2
PF02840 Pfam IPR004098 Prp18 195 336 1
SM00500 SMART IPR003648 Splicing factor motif 124 173 2
PTHR13007:SF19 HMMPANTHER 1 334 1
PTHR13007 HMMPANTHER 1 334 1
1dvkA00 Gene3D IPR004098 Prp18 176 337 1
0036629 SuperFamily IPR004098 Prp18 165 335 1
0053824 SuperFamily 111 159 2
Coil ncoils Rabaptin coiled-coil domain 1 22 968
Coil ncoils Rabaptin coiled-coil domain 41 79 968

View domain organization at Pfam

Protein Properties

Ave. residue weight 117.48 Da
Charge 7.50
Isoelectric point 9.17
Molecular weight 40.30 kDa
Number of residues 343
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS115 1670
Annotation ExtensionEvidenceResidueReference
experimental evidence S115 PMID:24763107
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
236.25during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
926.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.5during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


External References
Database Identifier Description
NBRP SPCC126.14 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC126.14 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC126.14 BioGRID Interaction Datasets
Expression Viewer SPCC126.14 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC126.14 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC126.14 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC126.14 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC126.14 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC126.14 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC126.14 Cell Cycle Data
GEO SPCC126.14 GEO profiles
PInt SPCC126.14 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC126.14 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC126.14 Fission yeast phenotypic data & analysis
SPD / RIKEN16/16F08Orfeome Localization Data
UniProtKB/SwissProtO94406Pre-mRNA-splicing factor 18
ModBaseO94406Database of comparative protein structure models
STRINGO94406Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588457U5 snRNP-associated protein Prp18 (predicted)
RefSeq mRNANM_001023448972h- U5 snRNP-associated protein Prp18 (predicted) (prp18), mRNA
European Nucleotide ArchiveBAA87243.1ENA Protein Mapping
European Nucleotide ArchiveCAA22483.1ENA Protein Mapping
UniParcUPI000006A369UniProt Archive

Literature for prp18

Search: Europe PMC or PubMed

Release Version: PomBase:25_49 - 02 Feb 2015