trp1322 (SPCC1322.03)


Gene Standard Nametrp1322 Characterisation Statuspublished
Systematic IDSPCC1322.03 Feature Typeprotein coding
Synonyms Name Description
ProductTRP-like ion channel (predicted) Product Size862aa, 97.44 kDa
Genomic Location Chromosome III, 1287903-1291811 (3909nt); CDS:1288137-1290725 (2589nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
transmembrane transporter activity291
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
cytoplasm4206
Annotation ExtensionEvidenceWith/FromReference
cytosol2316
Annotation ExtensionEvidenceWith/FromReference
Golgi apparatus357
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane1018
Annotation ExtensionEvidenceWith/FromReference
intracellular membrane-bounded organelle4093
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
normal growth during cellular response to salt stresstrp1322ΔNull13
normal growth on sorbitoltrp1322ΔNull17
normal growth on tacrolimustrp1322ΔNull5
normal growth on tacrolimus during osmotic stresstrp1322ΔNull3
resistance to tacrolimus during salt stresstrp1322ΔNull5
sensitive to amphotericin B69
expressivity FYPO_EXT:0000002trp1322ΔNull
sensitive to camptothecintrp1322ΔNull214
sensitive to micafungin116
expressivity FYPO_EXT:0000001trp1322ΔNull
viable vegetative cell populationtrp1322ΔNull3781

Cell Phenotype

Term NameAlleleExpressionCount
abnormal microtubule cytoskeleton morphology during mitotic interphase186
penetrance FYPO_EXT:0000002trp1322ΔNull
abnormal mitotic cell cycle phase114
penetrance FYPO_EXT:0000002trp1322ΔNull
decreased cellular calcium leveltrp1322ΔNull6
decreased cellular calcium level during cellular response to salt stressSPCC1322.03+ (wild type)Overexpression1
trp1322ΔNull
increased basal transcription from CDRE promoterSPCC1322.03+ (wild type)Overexpression8
increased duration of positive regulation of transcription from CDRE promoter in response to calcium ionSPCC1322.03+ (wild type)Overexpression2
increased immediate intracellular calcium spike following extracellular calcium stimulusSPCC1322.03+ (wild type)Overexpression2
normal cellular calcium levelSPCC1322.03+ (wild type)Overexpression9
normal cellular calcium level during cellular response to salt stressSPCC1322.03+ (wild type)Overexpression2
normal increase in steady-state intracellular calcium level following extracellular calcium stimulusSPCC1322.03+ (wild type)Overexpression1
short cytoplasmic microtubules13
penetrance FYPO_EXT:0000002, expressivity FYPO_EXT:0000003trp1322ΔNull
viable vegetative cell with abnormal cell shape472
penetrance FYPO_EXT:0000002trp1322ΔNull
viable vegetative cell with normal cell morphology3092
penetrance FYPO_EXT:0000001trp1322ΔNull
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
112879031291811

UTRs

Region Coordinates Reference
five_prime_UTR1287903..1288136PMID:21511999
three_prime_UTR1290726..1291811PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF06011 Pfam IPR010308 TRP-like family 188 588 3
PF14558 Pfam 27 182 3
TMhelix TMHMM 262 281 959
TMhelix TMHMM 63 85 959
TMhelix TMHMM 320 351 959
TMhelix TMHMM 546 568 959
TMhelix TMHMM 484 503 959
TMhelix TMHMM 7 29 959
TMhelix TMHMM 371 393 959
TMhelix TMHMM 458 480 959
TMhelix TMHMM 408 430 959
TMhelix TMHMM 231 250 959
TMhelix TMHMM 187 209 959
PTHR31145:SF3 HMMPANTHER 1 862 1
PTHR31145 HMMPANTHER 1 862 3
SignalP-noTM signalp 1 25 212

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0000418signal_peptide279
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 113.04 Da
Charge 15.00
Isoelectric point 8.77
Molecular weight 97.44 kDa
Number of residues 862
Sequence
Gene Expression

Quantitative Gene Expression

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.3during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog597
conserved in fungi only619
conserved in fungi4604
conserved in eukaryotes4516
Orthologs

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
apm1AP-1 adaptor complex mu subunit Apm1 Dosage RescuePMID:22848669
cis4cation diffusion family zinc transmembrane transporter Cis4 Dosage RescuePMID:22848669
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
xap5xap-5-like protein Negative GeneticPMID:24957674
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
External References
Database Identifier Description
NBRP SPCC1322.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1322.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1322.03 BioGRID Interaction Datasets
Expression Viewer SPCC1322.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1322.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1322.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1322.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1322.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1322.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC1322.03 Cell Cycle Data
GEO SPCC1322.03 GEO profiles
PInt SPCC1322.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC1322.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC1322.03 Fission yeast phenotypic data & analysis
SPD / RIKEN38/38E06Orfeome Localization Data
UniProtKB/SwissProtO94543Uncharacterized membrane protein C1322.03
ModBaseO94543Database of comparative protein structure models
STRINGO94543Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588131TRP-like ion channel (predicted)
RefSeq mRNANM_001023121972h- TRP-like ion channel (predicted) (SPCC1322.03), mRNA
European Nucleotide ArchiveCAA22856.1ENA Protein Mapping
UniParcUPI000006B86AUniProt Archive

Literature for trp1322

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015