trp1322 (SPCC1322.03)


Gene Standard Nametrp1322 Characterisation Statuspublished
Systematic IDSPCC1322.03 Feature Typeprotein coding
Synonyms Name Description
ProductTRP-like ion channel (predicted) Product Size862aa, 97.44 kDa
Genomic Location Chromosome III, 1287903-1291811 (3909nt); CDS:1288137-1290725 (2589nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
transmembrane transporter activity293
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
calcium ion transmembrane import into cytosol4
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4209
Annotation ExtensionEvidenceWith/FromReference
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
Golgi apparatus357
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane1018
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
resistance to tacrolimus during salt stresstrp1322ΔNull5
sensitive to amphotericin Btrp1322ΔNull69
sensitive to camptothecintrp1322ΔNull225
sensitive to micafungintrp1322ΔNull116
viable vegetative cell populationtrp1322ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
abnormal microtubule cytoskeleton morphology during mitotic interphasetrp1322ΔNull186
abnormal mitotic cell cycle phasetrp1322ΔNull142
increased cytosolic calcium leveltrp1322+ (wild type)Overexpression6
increased transcription from CDRE promoter in response to calcium iontrp1322+ (wild type)Overexpression6
short cytoplasmic microtubulestrp1322ΔNull15
viable vegetative cell with abnormal cell shapetrp1322ΔNull482
viable vegetative cell with normal cell morphologytrp1322ΔNull3094
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1287903..1291811
mRNA1287903..1291811
5' UTR1287903..1288136PMID:21511999
CDS1288137..1290725
3' UTR1290726..1291811PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF14558 Pfam 27 182 3
PF06011 Pfam IPR010308 TRP-like family 188 588 3
TMhelix TMHMM 262 281 959
TMhelix TMHMM 231 250 959
TMhelix TMHMM 546 568 959
TMhelix TMHMM 371 393 959
TMhelix TMHMM 187 209 959
TMhelix TMHMM 320 351 959
TMhelix TMHMM 63 85 959
TMhelix TMHMM 458 480 959
TMhelix TMHMM 408 430 959
TMhelix TMHMM 7 29 959
TMhelix TMHMM 484 503 959
PTHR31145:SF3 HMMPANTHER 1 862 1
PTHR31145 HMMPANTHER 1 862 3
SignalP-noTM signalp 1 25 212

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0000418signal_peptide279
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 113.04 Da
Charge 15.00
Isoelectric point 8.77
Molecular weight 97.44 kDa
Number of residues 862
Sequence
Gene Expression

Quantitative Gene Expression

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.3during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog599
conserved in fungi only620
conserved in fungi4603
conserved in eukaryotes4514
Orthologs

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
overexpression rescuesapm1AP-1 adaptor complex mu subunit Apm1 Dosage RescuePMID:22848669
overexpression rescuescis4cation diffusion family zinc transmembrane transporter Cis4 Dosage RescuePMID:22848669
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
negative genetic interaction withdsc4Golgi Dsc E3 ligase complex subunit Dsc4 Negative GeneticPMID:21504829
overexpression rescuesppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Dosage RescuePMID:21811607
negative genetic interaction withxap5xap-5-like protein Negative GeneticPMID:24957674
External References
Database Identifier Description
NBRP SPCC1322.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1322.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1322.03 BioGRID Interaction Datasets
Expression Viewer SPCC1322.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1322.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1322.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1322.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1322.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1322.03 Transcriptome Viewer (Bähler Lab)
GEO SPCC1322.03 GEO profiles
PInt SPCC1322.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC1322.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC1322.03 Fission yeast phenotypic data & analysis
Cyclebase SPCC1322.03.1 Cell Cycle Data
SPD / RIKEN38/38E06Orfeome Localization Data
UniProtKB/SwissProtO94543Uncharacterized membrane protein C1322.03
ModBaseO94543Database of comparative protein structure models
STRINGO94543Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588131TRP-like ion channel (predicted)
RefSeq mRNANM_001023121972h- TRP-like ion channel (predicted) (SPCC1322.03), mRNA
European Nucleotide ArchiveCAA22856.1ENA Protein Mapping
UniParcUPI000006B86AUniProt Archive

Literature for trp1322

Search: Europe PMC or PubMed

Release Version: PomBase:26_52 - 11 May 2015