trp1322 (SPCC1322.03)


Gene Standard Nametrp1322 Characterisation Statuspublished
Systematic IDSPCC1322.03 Feature Typeprotein coding
Synonyms Name Description
ProductTRP-like ion channel (predicted) Product Size862aa, 97.44 kDa
Genomic Location Chromosome III, 1287903-1291811 (3909nt); CDS:1288137-1290725 (2589nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0022857transmembrane transporter activityNASGO_REF:0000051290
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0060402calcium ion transport into cytosolIMPPMID:218116072
GO:1901200negative regulation of calcium ion transport into cytosol involved in cellular response to salt stressIMPPMID:218116071
GO:1901198positive regulation of calcium ion transport into cytosol involved in cellular response to calcium ionIMPPMID:218116071
GO:0055085transmembrane transportNASGO_REF:0000051323
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005794Golgi apparatusIDAPMID:16823372355
GO:0005737cytoplasmIDAPMID:168233724201
GO:0016021integral component of membraneIEAUniProtKB-KW:KW-0812GO_REF:00000371017
GO:0043231intracellular membrane-bounded organelleIDAPMID:218116074123
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001037normal growth during cellular response to salt stressCell growth assaytrp1322ΔNullPECO:0000031, PECO:0000005, PECO:0000102PMID:218116079
Cell growth assaytrp1322ΔNullPECO:0000092, PECO:0000005, PECO:0000102PMID:21811607
Cell growth assaytrp1322ΔNullPECO:0000038, PECO:0000102, PECO:0000005PMID:21811607
FYPO:0000961normal growth on sorbitolCell growth assaytrp1322ΔNullPECO:0000102, PECO:0000005PMID:2181160710
FYPO:0001470normal growth on tacrolimusCell growth assaytrp1322ΔNullPECO:0000005, PECO:0000102PMID:218116075
FYPO:0001472normal growth on tacrolimus during osmotic stressCell growth assaytrp1322ΔNullPECO:0000005, PECO:0000102, PECO:0000025PMID:218116073
FYPO:0001473resistance to tacrolimus during salt stressCell growth assaytrp1322ΔNullPECO:0000005, PECO:0000102PMID:218116075
FYPO:0002642sensitive to amphotericin B69
expressivity FYPO_EXT:0000002Cell growth assaytrp1322ΔNullPECO:0000137, PECO:0000005PMID:23738021
FYPO:0000085sensitive to camptothecincompetitive growth assay evidencetrp1322ΔNullPMID:20537132211
FYPO:0002641sensitive to micafungin108
expressivity FYPO_EXT:0000001Cell growth assaytrp1322ΔNullPECO:0000137, PECO:0000005PMID:23738021
FYPO:0002060viable vegetative cell populationMicroscopytrp1322ΔNullPECO:0000005, PECO:0000137PMID:236978063760
Microscopytrp1322ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001197decreased cellular calcium levelSubstance quantificationtrp1322ΔNullPMID:218116076
Substance quantificationtrp1322ΔNullPECO:0000160PMID:21811607
FYPO:0001377decreased cellular calcium level during cellular response to salt stressSubstance quantificationSPCC1322.03+ (wild type)OverexpressionPECO:0000160PMID:218116071
Reporter gene assaySPCC1322.03+ (wild type)OverexpressionPMID:21811607
Reporter gene assaytrp1322ΔNullPECO:0000160PMID:21811607
Reporter gene assaytrp1322ΔNullPMID:21811607
FYPO:0001459increased basal transcription from CDRE promoterReporter gene assaySPCC1322.03+ (wild type)OverexpressionPMID:218116072
Reporter gene assaySPCC1322.03+ (wild type)OverexpressionPMID:21811607
Reporter gene assaySPCC1322.03+ (wild type)OverexpressionPMID:21811607
FYPO:0001460increased duration of positive regulation of transcription from CDRE promoter in response to calcium ionReporter gene assaySPCC1322.03+ (wild type)OverexpressionPECO:0000034PMID:218116071
FYPO:0001464increased immediate intracellular calcium spike following extracellular calcium stimulusSubstance quantificationSPCC1322.03+ (wild type)OverexpressionPMID:218116072
FYPO:0001199normal cellular calcium levelSubstance quantificationSPCC1322.03+ (wild type)OverexpressionPECO:0000160PMID:218116079
FYPO:0001376normal cellular calcium level during cellular response to salt stressSubstance quantificationSPCC1322.03+ (wild type)OverexpressionPMID:218116072
FYPO:0001467normal increase in steady-state intracellular calcium level following extracellular calcium stimulusSubstance quantificationSPCC1322.03+ (wild type)OverexpressionPMID:218116071
FYPO:0002177viable vegetative cell with normal cell morphology3086
penetrance FYPO_EXT:0000001Microscopytrp1322ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
112879031291811

UTRs

Region Coordinates Reference
five_prime_UTR1287903..1288136PMID:21511999
three_prime_UTR1290726..1291811PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF14558 Pfam 27 182 3
PF06011 Pfam IPR010308 TRP-like family 188 588 3
TMhelix TMHMM 63 85 959
TMhelix TMHMM 484 503 959
TMhelix TMHMM 187 209 959
TMhelix TMHMM 262 281 959
TMhelix TMHMM 458 480 959
TMhelix TMHMM 408 430 959
TMhelix TMHMM 320 351 959
TMhelix TMHMM 231 250 959
TMhelix TMHMM 371 393 959
TMhelix TMHMM 7 29 959
TMhelix TMHMM 546 568 959
PTHR31145:SF3 HMMPANTHER 1 862 1
PTHR31145 HMMPANTHER 1 862 3
SignalP-noTM signalp 1 25 212

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0000418signal_peptide278
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 113.04 Da
Charge 15.00
Isoelectric point 8.77
Molecular weight 97.44 kDa
Number of residues 862
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
NDduring GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
1.3during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog580
conserved in fungi only603
conserved in fungi4569
conserved in eukaryotes4482
Orthologs

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
apm1AP-1 adaptor complex mu subunit Apm1 Dosage RescuePMID:22848669
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
cis4cation diffusion family zinc membrane transporter Cis4 Dosage RescuePMID:22848669
External References
Database Identifier Description
NBRP SPCC1322.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1322.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1322.03 BioGRID Interaction Datasets
Expression Viewer SPCC1322.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1322.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1322.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1322.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1322.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1322.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC1322.03 Cell Cycle Data
GEO SPCC1322.03 GEO profiles
PInt SPCC1322.03 Protein-Protein Interaction Predictor (Bähler Lab)
EntrezGene2539071TRP-like ion channel
WikiGene2539071TRP-like ion channel
SPD / RIKEN38/38E06Orfeome Localization Data
UniProtKB/SwissProtO94543Uncharacterized membrane protein C1322.03
ModBaseO94543Database of comparative protein structure models
STRINGO94543Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588131TRP-like ion channel
RefSeq mRNANM_001023121972h- TRP-like ion channel (SPCC1322.03), mRNA
European Nucleotide ArchiveCAA22856ENA Protein Mapping
European Nucleotide ArchiveCAA22856.1ENA Protein Mapping
UniParcUPI000006B86AUniProt Archive

Literature for trp1322

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014