bub1 (SPCC1322.12c)


Gene Standard Namebub1 Characterisation Statuspublished
Systematic IDSPCC1322.12c Feature Typeprotein coding
Synonyms Name Description
Productmitotic spindle checkpoint kinase Bub1 Product Size1044aa, 118.35 kDa
Genomic Location Chromosome III, 1316005-1312560 (3446nt); CDS:1315932-1312798 (3135nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
histone kinase activity (H2A-S121 specific)1
Annotation ExtensionEvidenceWith/FromReference
protein binding851
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
attachment of mitotic spindle microtubules to kinetochore39
Annotation ExtensionEvidenceWith/FromReference
cellular protein localization553
Annotation ExtensionEvidenceWith/FromReference
histone H2A-S121 phosphorylation1
Annotation ExtensionEvidenceWith/FromReference
meiotic sister chromatid cohesion, centromeric6
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle assembly checkpoint13
Annotation ExtensionEvidenceWith/FromReference
protein localization to kinetochore18
Annotation ExtensionEvidenceWith/FromReference
signal transduction involved in mitotic spindle assembly checkpoint5
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
bub1-bub3 complex2
Annotation ExtensionEvidenceWith/FromReference
condensed nuclear chromosome kinetochore58
Annotation ExtensionEvidenceWith/FromReference
nucleus2697
Annotation ExtensionEvidenceWith/FromReference
old mitotic spindle pole body4
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
sensitive to thiabendazolebub1ΔNull191
viable vegetative cell populationbub1ΔNull3783
unnamed (unrecorded)

Cell Phenotype

Term NameAlleleExpressionCount
abnormal meiotic chromosome segregationbub1ΔNull34
increased minichromosome loss during vegetative growthbub1ΔNull43
viable vegetative cell with normal cell morphologybub1ΔNull3092
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in bub3 mitotic spindle checkpoint protein Bub3 PMID:22521786
FYPO affected by mutation in cid12 poly(A) polymerase Cid12 PMID:16738311
FYPO affected by mutation in dam1 DASH complex subunit Dam1 PMID:16079915
FYPO affected by mutation in dis3 3'-5' exoribonuclease subunit Dis3 PMID:17380189
FYPO affected by mutation in mal3 EB1 family Mal3 PMID:16179942
FYPO affected by mutation in mph1 dual specificity protein kinase Mph1 PMID:22521786
FYPO affected by mutation in msd1 mitotic-spindle disanchored Msd1 PMID:17486116
FYPO affected by mutation in rdp1 RNA-directed RNA polymerase Rdp1 PMID:16738311
FYPO affected by mutation in spc7 NMS complex subunit, Spc105/KNL-1 family member, blinkin, Spc7 PMID:22521786
GO localized by bub3 mitotic spindle checkpoint protein Bub3 PMID:15509783
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
113160051312560

UTRs

Region Coordinates Reference
five_prime_UTR1316005..1315933PMID:21511999
three_prime_UTR1312797..1312560PMID:21511999
exon1315932..1312798
mRNA1316005..1312560
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08311 Pfam IPR013212 Mad3/BUB1 homology region 1 31 159 2
PF00069 Pfam IPR000719 Protein kinase domain 720 984 109
SM00220 SMART IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain 718 1022 107
SM00777 SMART IPR013212 Mad3/BUB1 homology region 1 29 159 2
PS50011 Prosite Profiles IPR000719 Protein kinase domain 718 1022 110
PS51489 Prosite Profiles IPR013212 Mad3/BUB1 homology region 1 36 204 2
PTHR14030 HMMPANTHER IPR015661 Mitotic checkpoint serine/threonine protein kinase Bub1/Mitotic spindle checkpoint component Mad3 16 1040 2
PTHR14030:SF4 HMMPANTHER 16 1040 1
3.30.200.20 Gene3D 716 857 111
1.10.510.10 Gene3D 858 908 112
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 717 981 127

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.37 Da
Charge -6.00
Isoelectric point 6.21
Molecular weight 118.35 kDa
Number of residues 1044
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS381
present during mitotic M phaseS316
present during mitotic M phaseS323
present during mitotic M phaseS532
present during mitotic M phaseS600
present during mitotic M phaseS321
present during mitotic M phaseS338
present during mitotic M phase, cellular response to thiabendazoleS545
S372, S412, S415, S532, S600
present during mitotic M phaseS372
present during mitotic M phaseS403
present during mitotic M phaseS405
present during mitotic M phaseS558
present during mitotic M phaseS327
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S316 PMID:21712547
present during mitotic M phase experimental evidence S321 PMID:21712547
present during mitotic M phase experimental evidence S323 PMID:21712547
present during mitotic M phase experimental evidence S327 PMID:21712547
present during mitotic M phase experimental evidence S338 PMID:21712547
experimental evidence S372 PMID:24763107
present during mitotic M phase experimental evidence S372 PMID:21712547
present during mitotic M phase experimental evidence S381 PMID:21712547
present during mitotic M phase experimental evidence S403 PMID:21712547
present during mitotic M phase experimental evidence S405 PMID:21712547
experimental evidence S412 PMID:24763107
experimental evidence S415 PMID:24763107
experimental evidence S532 PMID:24763107
present during mitotic M phase experimental evidence S532 PMID:21712547
present during mitotic M phase experimental evidence S545 PMID:21712547
present during cellular response to thiabendazole IDA S545 PMID:18257517
present during mitotic M phase experimental evidence S558 PMID:21712547
experimental evidence S600 PMID:24763107
present during mitotic M phase experimental evidence S600 PMID:21712547
O-phospho-L-threonineT342 694
present during mitotic M phaseT534
present during mitotic M phaseT386
present during mitotic M phaseT541
Annotation ExtensionEvidenceResidueReference
experimental evidence T342 PMID:24763107
present during mitotic M phase experimental evidence T386 PMID:21712547
present during mitotic M phase experimental evidence T534 PMID:21712547
present during mitotic M phase experimental evidence T541 PMID:21712547
phosphorylated residue 1923
Annotation ExtensionEvidenceResidueReference
NAS PMID:18257517
IDA PMID:19547744
O-phosphorylated residueS372 1751
Annotation ExtensionEvidenceResidueReference
IDA S372 PMID:23297348
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1093during GO:0000085PECO:0000005,
PECO:0000126
single cellexperimental evidencePMID:24161933
785during GO:0007067PECO:0000005,
PECO:0000126
single cellexperimental evidencePMID:24161933
511.03during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1961.71during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.6during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
9.2during GO:0072690PECO:0000005,
PECO:0000126
single cellin situ hybridization assay evidencePMID:24161933
0.67during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancer142
Pathway
DescriptionQualifierReferenceCount
Mad2-dependent checkpoint response7
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withbub1mitotic spindle checkpoint kinase Bub1 Two-hybridPMID:23695164
forms complex withbub3mitotic spindle checkpoint protein Bub3 Reconstituted ComplexPMID:22660415
affinity captured bybub3mitotic spindle checkpoint protein Bub3 Affinity Capture-WesternPMID:15525673
affinity capturesbub3mitotic spindle checkpoint protein Bub3 Affinity Capture-WesternPMID:15525673
affinity captured bybub3mitotic spindle checkpoint protein Bub3 Affinity Capture-WesternPMID:15509783
affinity capturesbub3mitotic spindle checkpoint protein Bub3 Affinity Capture-WesternPMID:15509783
modified bycdc2cyclin-dependent protein kinase Cdk1/Cdc2 Biochemical ActivityPMID:12606573
affinity captured byclp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
binds activation domain construct withmad3mitotic spindle checkpoint protein Mad3 Two-hybridPMID:23695164
affinity captured bypir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
forms complex withspc7NMS complex subunit, Spc105/KNL-1 family member, blinkin, Spc7 Reconstituted ComplexPMID:22660415
affinity capturesspc7NMS complex subunit, Spc105/KNL-1 family member, blinkin, Spc7 Affinity Capture-WesternPMID:22521786
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
rescuesalp5actin-like protein Arp4 Phenotypic SuppressionPMID:15483052
phenotype enhanced byarc3ARP2/3 actin-organizing complex subunit Arc21 Phenotypic EnhancementPMID:20668161
synthetic lethal withatb2tubulin alpha 2 Synthetic LethalityPMID:16394105
phenotype enhanced bybgs11,3-beta-glucan synthase catalytic subunit Bgs1 Phenotypic EnhancementPMID:15146064
synthetic growth defect withcid12poly(A) polymerase Cid12 Synthetic Growth DefectPMID:16738311
synthetic lethal withcid14poly(A) polymerase Cid14 Synthetic LethalityPMID:16478992
synthetic lethal withcsi1mitotic chromosome segregation protein Csi1 Synthetic LethalityPMID:23166349
phenotype enhanced bydad2DASH complex subunit Dad2 Phenotypic EnhancementPMID:18632983
synthetic lethal withfin1serine/threonine protein kinase, NIMA related Fin1 Synthetic LethalityPMID:12065422
synthetic growth defect withfin1serine/threonine protein kinase, NIMA related Fin1 Synthetic Growth DefectPMID:11927555
synthetic growth defect withgtb1gamma-tubulin Gtb1 Synthetic Growth DefectPMID:17277378
synthetic growth defect withhrk1haspin related kinase Hrk1 Synthetic Growth DefectPMID:20929775
synthetic lethal withklp5kinesin-like protein Klp5 Synthetic LethalityPMID:11870212
synthetic lethal withklp6kinesin-like protein Klp6 Synthetic LethalityPMID:11870212
synthetic growth defect withmad2mitotic spindle checkpoint protein Mad2 Synthetic Growth DefectPMID:12606573
synthetic growth defect withmal3EB1 family Mal3 Synthetic Growth DefectPMID:16179942
synthetic growth defect withmcl1DNA polymerase alpha accessory factor Mcl1 Synthetic Growth DefectPMID:12455694
synthetic lethal withmde4monopolin-like complex subunit Mde4 Synthetic LethalityPMID:19523829
phenotype enhanced bymph1dual specificity protein kinase Mph1 Phenotypic EnhancementPMID:22825872
synthetically rescuesmph1dual specificity protein kinase Mph1 Synthetic RescuePMID:22184248
phenotype enhanced bymph1dual specificity protein kinase Mph1 Phenotypic EnhancementPMID:22281223
synthetic growth defect withmph1dual specificity protein kinase Mph1 Synthetic Growth DefectPMID:22281223
overexpression causes growth defect topds5mitotic cohesin-associated protein Pds5 Dosage Growth DefectPMID:20929775
synthetic growth defect withpfs2WD repeat protein Pfs2 Synthetic Growth DefectPMID:15743824
rescuespot1telomere end-binding protein Pot1 Phenotypic SuppressionPMID:24469396
synthetic growth defect withpot1telomere end-binding protein Pot1 Synthetic Growth DefectPMID:24469396
rescuesrqh1RecQ type DNA helicase Rqh1 Phenotypic SuppressionPMID:24469396
synthetic growth defect withrqh1RecQ type DNA helicase Rqh1 Synthetic Growth DefectPMID:24469396
synthetic growth defect withscm3CENP-A chaperone Scm3 Synthetic Growth DefectPMID:12719471
synthetic growth defect withsgo2inner centromere protein, shugoshin Sgo2 Synthetic Growth DefectPMID:14730319
synthetic growth defect withsim3NASP family CENP-A chaperone Synthetic Growth DefectPMID:12719471
synthetic growth defect withswi6HP1 family chromodomain protein Swi6 Synthetic Growth DefectPMID:14730319
synthetic lethal withswi6HP1 family chromodomain protein Swi6 Synthetic LethalityPMID:9864354
synthetic growth defect withtaz1human TRF ortholog Taz1 Synthetic Growth DefectPMID:12620220
External References
Database Identifier Description
NBRP SPCC1322.12c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1322.12c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1322.12c BioGRID Interaction Datasets
Expression Viewer SPCC1322.12c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1322.12c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1322.12c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1322.12c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1322.12c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1322.12c Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC1322.12c Cell Cycle Data
GEO SPCC1322.12c GEO profiles
PInt SPCC1322.12c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC1322.12c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC1322.12c Fission yeast phenotypic data & analysis
SPD / RIKEN30/30A12Orfeome Localization Data
UniProtKB/SwissProtO94751Checkpoint serine/threonine-protein kinase bub1
ModBaseO94751Database of comparative protein structure models
STRINGO94751Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588140serine/threonine protein kinase Bub1
RefSeq mRNANM_001023130972h- serine/threonine protein kinase Bub1 (bub1), mRNA
European Nucleotide ArchiveAAC98348.1ENA Protein Mapping
European Nucleotide ArchiveCAA22865.1ENA Protein Mapping
UniParcUPI0000126B2DUniProt Archive

Literature for bub1

Search: Europe PMC or PubMed

Release Version: PomBase:25_49 - 02 Feb 2015