bub1 (SPCC1322.12c)


Gene Standard Namebub1 Characterisation Statuspublished
Systematic IDSPCC1322.12c Feature Typeprotein coding
Synonyms Name Description
Productserine/threonine protein kinase Bub1 Product Size1044aa, 118.35 kDa
Genomic Location Chromosome III, 1316005-1312560 (3446nt); CDS:1315932-1312798 (3135nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037559
GO:0072371histone kinase activity (H2A-S121 specific)IDAPMID:199653871
has substrate hta1IDAPMID:19965387
has substrate hta2IDAPMID:19965387
GO:0005515protein bindingIPIbub3PMID:15525673887
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0008608attachment of spindle microtubules to kinetochoreIGImal3PMID:1639410544
GO:0051455attachment of spindle microtubules to kinetochore involved in homologous chromosome segregationIMPPMID:1996538712
GO:0034613cellular protein localization556
localizes bub3IMPPMID:15509783
localizes mad3IMPPMID:15525673
GO:0072370histone H2A-S121 phosphorylationIDAPMID:199653871
GO:0051754meiotic sister chromatid cohesion, centromericIGIsgo1PMID:147303195
GO:0007094mitotic spindle assembly checkpointIMPPMID:986435428
GO:0034501protein localization to kinetochore16
localizes sgo2IMPPMID:14730319
localizes sgo2IMPPMID:19965387
localizes sgo1IMPPMID:14730319
GO:0007165signal transductionNASGO_REF:0000051287
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000778condensed nuclear chromosome kinetochoreIDAPMID:1473031953
GO:0005634nucleus2730
predominantlyIDAPMID:15525673
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopybub1ΔNullPMID:204732893755
no_namePMID:11331883
Microscopybub1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000151abnormal meiotic chromosome segregationMicroscopybub1ΔNullPMID:2337039210
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopybub1ΔNullPECO:0000005, PECO:0000137PMID:236978063078
penetrance FYPO_EXT:0000001
Target Of
RelationshipGeneProductReference
localized by bub3 mitotic spindle checkpoint protein Bub3 PMID:15509783
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
113160051312560

UTRs

Region Start End Reference
three_prime_UTR13127971312560PMID:21511999
five_prime_UTR13160051315933PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 720 984 109
PF08311 Pfam IPR013212 Mad3/BUB1 homology region 1 31 159 2
SM00777 SMART IPR013212 Mad3/BUB1 homology region 1 29 159 2
PS51489 Prosite Profiles IPR013212 Mad3/BUB1 homology region 1 36 204 2
PS50011 Prosite Profiles IPR000719 Protein kinase domain 718 1022 110
PTHR14030:SF0 HMMPANTHER 19 1042 2
PTHR14030 HMMPANTHER IPR015661 Mitotic checkpoint serine/threonine protein kinase Bub1/Mitotic spindle checkpoint component Mad3 19 1042 2
G3DSA:3.30.200.20 Gene3D 716 857 109
G3DSA:1.10.510.10 Gene3D 858 908 112
SSF56112 SuperFamily IPR011009 717 981 127
Low complexity (SEG) seg 504 518
Low complexity (SEG) seg 1028 1038
Low complexity (SEG) seg 38 48
Low complexity (SEG) seg 316 327
Low complexity (SEG) seg 400 419

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.37 Da
Charge -6.00
Isoelectric point 6.21
Molecular weight 118.35 kDa
Number of residues 1044
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residuePMID:182575171192
PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
511.03during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1961.71during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2.6during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.67during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancer141
Pathway
DescriptionQualifierReferenceCount
Mad2-dependent checkpoint response7
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
taz1human TRF ortholog Taz1 Synthetic Growth DefectPMID:12620220
csi1chromosome segregation impaired protein 1 Synthetic LethalityPMID:23166349
mph1dual specificity protein kinase Mph1 Phenotypic EnhancementPMID:22825872
Synthetic RescuePMID:22184248
Synthetic Growth DefectPMID:22281223
Phenotypic Enhancement
klp6kinesin-like protein Klp6 Synthetic LethalityPMID:11870212
scm3CENP-A chaperone Scm3 Synthetic Growth DefectPMID:12719471
mad2spindle checkpoint protein Mad2 Synthetic Growth DefectPMID:12606573
arc3ARP2/3 actin-organizing complex subunit Arc21 Phenotypic EnhancementPMID:20668161
cid12poly(A) polymerase Cid12 Synthetic Growth DefectPMID:16738311
mal3EB1 family Mal3 Synthetic Growth DefectPMID:16179942
cid14poly(A) polymerase Cid14 Synthetic LethalityPMID:16478992
gtb1gamma-tubulin Gtb1 Synthetic Growth DefectPMID:17277378
mde4monopolin-like complex subunit Mde4 Synthetic LethalityPMID:19523829
pds5cohesin-associated protein Pds5 Dosage Growth DefectPMID:20929775
alp5actin-like protein Arp4 Phenotypic SuppressionPMID:15483052
sim3NASP family CENP-A chaperone Synthetic Growth DefectPMID:12719471
sgo2inner centromere protein, shugoshin Sgo2 Synthetic Growth DefectPMID:14730319
dad2DASH complex subunit Dad2 Phenotypic EnhancementPMID:18632983
hrk1haspin related kinase Hrk1 Synthetic Growth DefectPMID:20929775
fin1serine/threonine protein kinase, NIMA related Fin1 Synthetic LethalityPMID:12065422
Synthetic Growth DefectPMID:11927555
mcl1DNA polymerase alpha accessory factor Mcl1 Synthetic Growth DefectPMID:12455694
atb2tubulin alpha 2 Synthetic LethalityPMID:16394105
bgs11,3-beta-glucan synthase catalytic subunit Bgs1 Phenotypic EnhancementPMID:15146064
klp5kinesin-like protein Klp5 Synthetic LethalityPMID:11870212
pfs2WD repeat protein Pfs2 Synthetic Growth DefectPMID:15743824
swi6HP1 family chromodomain protein Swi6 Synthetic Growth DefectPMID:14730319
Synthetic LethalityPMID:9864354
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
mad3mitotic spindle checkpoint protein Mad3 Two-hybridPMID:23695164
bub3mitotic spindle checkpoint protein Bub3 Reconstituted ComplexPMID:22660415
Affinity Capture-WesternPMID:15525673
Affinity Capture-WesternPMID:15509783
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Biochemical ActivityPMID:12606573
spc7kinetochore protein Spc7 Reconstituted ComplexPMID:22660415
External References
Database Identifier Description
NBRP SPCC1322.12c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1322.12c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1322.12c BioGRID Interaction Datasets
Expression Viewer SPCC1322.12c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1322.12c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1322.12c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1322.12c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1322.12c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1322.12c Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC1322.12c Cell Cycle Data
GEO SPCC1322.12c GEO profiles
PInt SPCC1322.12c Protein-Protein Interaction Predictor (Bähler Lab)
SPD / RIKEN30/30A12Orfeome Localization Data
WikiGene2538736serine/threonine protein kinase Bub1
EntrezGene2538736serine/threonine protein kinase Bub1
UniProtKB/SwissProtO94751Checkpoint serine/threonine-protein kinase bub1
ModBaseO94751Database of comparative protein structure models
StringO94751Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588140serine/threonine protein kinase Bub1
RefSeq mRNANM_001023130972h- serine/threonine protein kinase Bub1 (bub1), mRNA
European Nucleotide ArchiveAAC98348ENA Protein Mapping
European Nucleotide ArchiveCAA22865ENA Protein Mapping
UniParcUPI0000126B2DUniProt Archive

Literature for bub1

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014