bub1 (SPCC1322.12c)


Gene Standard Namebub1 Characterisation Statuspublished
Systematic IDSPCC1322.12c Feature Typeprotein coding
Synonyms Name Description
Productmitotic spindle checkpoint kinase Bub1 Product Size1044aa, 118.35 kDa
Genomic Location Chromosome III, 1316005-1312560 (3446nt); CDS:1315932-1312798 (3135nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding559
Annotation ExtensionEvidenceWith/FromReference
histone kinase activity (H2A-S121 specific)1
Annotation ExtensionEvidenceWith/FromReference
protein binding863
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
attachment of mitotic spindle microtubules to kinetochore39
Annotation ExtensionEvidenceWith/FromReference
cellular protein localization508
Annotation ExtensionEvidenceWith/FromReference
histone H2A-S121 phosphorylation1
Annotation ExtensionEvidenceWith/FromReference
meiotic sister chromatid cohesion, centromeric5
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle assembly checkpoint10
Annotation ExtensionEvidenceWith/FromReference
protein localization to kinetochore17
Annotation ExtensionEvidenceWith/FromReference
signal transduction involved in mitotic spindle assembly checkpoint4
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
bub1-bub3 complex2
Annotation ExtensionEvidenceWith/FromReference
condensed nuclear chromosome kinetochore60
Annotation ExtensionEvidenceWith/FromReference
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
old mitotic spindle pole body4
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
normal cell population growth at high temperaturebub1Δ66
normal cell population growth at low temperaturebub1Δ29
normal growth on glucose carbon sourcebub1Δ273
sensitive to thiabendazolebub1Δ264
viable vegetative cell populationbub1Δ3837

Cell Phenotype

Term NameGenotypesCount
abnormal meiotic chromosome segregationbub1Δ46
increased minichromosome loss during vegetative growthbub1Δ66
unequal mitotic sister chromatid segregation following normal mitosisbub1Δ6
viable vegetative cell with normal cell morphologybub1Δ3100

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased cell population growth on glucose carbon sourcecsp10-439, bub1Δ, dma1Δ
bub1Δ, csp10-439
decreased vegetative cell population growthbub1-KD (K762R, D900N), pds5Δ
pds5Δ, bub1-KD (K762R, D900N), bir1-CD (bir1 C-terminus fused to chromodomain)
mcl1-1 (ntG372A), bub1Δ
mcl1+, bub1Δ
bub1-KD (K762R, D900N), hrk1Δ
bir1-CD (bir1 C-terminus fused to chromodomain), bub1-KD (K762R, D900N), hrk1Δ
inviable vegetative cell populationbub1Δ, cid14Δ
loss of viability at high temperaturemcl1-1 (ntG372A), bub1Δ
bub1Δ, pfs2-11
bub1Δ, mcl1+
sensitive to thiabendazolerna14-11 (R316Q), bub1Δ
bub1Δ, csp10-439
csp10-439, bub1Δ, dma1Δ

Cell Phenotype

Term NameGenotypes
abnormal mitotic sister chromatid segregation with lagging chromosomes and decreased rate of mitotic spindle elongationbub1Δ, csp10-439, dma1Δ
bub1Δ, csp10-439
abolished mitotic cell cycle spindle assembly checkpointalp5-1134 (S402N), bub1Δ
inviable mononucleate monoseptate vegetative cellrna14-11 (R316Q), bub1Δ
unequal mitotic sister chromatid segregationalp5-1134 (S402N), bub1Δ
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in msd1 microtubule-anchoring factor Msd1
FYPO affected by mutation in spc7 NMS complex subunit, Spc105/KNL-1 family member, blinkin, Spc7
FYPO localization affected by mutation in bub3 mitotic spindle checkpoint protein Bub3
FYPO localization affected by mutation in cid12 poly(A) polymerase Cid12
FYPO localization affected by mutation in dam1 DASH complex subunit Dam1
FYPO localization affected by mutation in dis3 3'-5' exoribonuclease subunit Dis3
FYPO localization affected by mutation in mal3 EB1 family Mal3
FYPO localization affected by mutation in mph1 dual specificity protein kinase Mph1
FYPO localization affected by mutation in ndc80 NMS complex subunit Ndc80
FYPO localization affected by mutation in rdp1 RNA-directed RNA polymerase Rdp1
FYPO localization affected by mutation in rna14 mRNA cleavage and polyadenylation specificity factor complex subunit Rna14 (predicted)
FYPO localization affected by mutation in spc7 NMS complex subunit, Spc105/KNL-1 family member, blinkin, Spc7
GO localized by bub3 mitotic spindle checkpoint protein Bub3
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1316005..1312560
5' UTR1316005..1315933PMID:21511999
3' UTR1312797..1312560PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08311 Pfam IPR013212 Mad3/Bub1 homology region 1 34 158 2
PF00069 Pfam IPR000719 Protein kinase domain 752 962 108
SM00220 SMART IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain 718 1022 107
SM00777 SMART IPR013212 Mad3/Bub1 homology region 1 29 159 2
PS51489 Prosite Profiles IPR013212 Mad3/Bub1 homology region 1 36 204 2
PS50011 Prosite Profiles IPR000719 Protein kinase domain 718 1022 110
PTHR14030 HMMPANTHER IPR015661 Mitotic spindle checkpoint protein Bub1/Mad3 13 1040 2
PTHR14030:SF4 HMMPANTHER 13 1040 1
1.10.510.10 Gene3D AGC-kinase, C-terminal 858 908 112
3.30.200.20 Gene3D AGC-kinase, C-terminal 716 857 111
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 717 981 127

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.37 Da
Charge -6.00
Codon Adaptation Index 0.42
Isoelectric point 6.21
Molecular weight 118.35 kDa
Number of residues 1044
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phaseS316
present during mitotic M phaseS372
present during cellular response to thiabendazole, mitotic M phaseS545
present during mitotic M phaseS327
present during mitotic M phaseS323
present during mitotic M phaseS321
present during mitotic M phaseS558
present during mitotic M phaseS403
present during mitotic M phaseS381
present during mitotic M phaseS532
present during mitotic M phaseS338
present during mitotic M phaseS405
present during mitotic M phaseS600
S372, S412, S415, S532, S600
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S316 PMID:21712547
present during mitotic M phase experimental evidence S372 PMID:21712547
present during cellular response to thiabendazole IDA S545 PMID:18257517
present during mitotic M phase experimental evidence S327 PMID:21712547
present during mitotic M phase experimental evidence S323 PMID:21712547
present during mitotic M phase experimental evidence S321 PMID:21712547
present during mitotic M phase experimental evidence S558 PMID:21712547
present during mitotic M phase experimental evidence S403 PMID:21712547
present during mitotic M phase experimental evidence S545 PMID:21712547
present during mitotic M phase experimental evidence S381 PMID:21712547
present during mitotic M phase experimental evidence S532 PMID:21712547
present during mitotic M phase experimental evidence S338 PMID:21712547
present during mitotic M phase experimental evidence S405 PMID:21712547
present during mitotic M phase experimental evidence S600 PMID:21712547
experimental evidence S372 PMID:24763107
experimental evidence S600 PMID:24763107
experimental evidence S415 PMID:24763107
experimental evidence S532 PMID:24763107
experimental evidence S412 PMID:24763107
O-phospho-L-threonine 700
present during mitotic M phaseT386
present during mitotic M phaseT534
present during mitotic M phaseT541
T342
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T386 PMID:21712547
present during mitotic M phase experimental evidence T534 PMID:21712547
present during mitotic M phase experimental evidence T541 PMID:21712547
experimental evidence T342 PMID:24763107
phosphorylated residue 1930
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
O-phosphorylated residueS372 1756
Annotation ExtensionEvidenceResidueReference
IDA S372 PMID:23297348
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for bub1 (SPCC1322.12c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1093during GO:0000085PECO:0000126,
PECO:0000005
single cellexperimental evidencePMID:24161933
511.03during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1961.71during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
785during GO:0007067PECO:0000126,
PECO:0000005
single cellexperimental evidencePMID:24161933

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
9.2during GO:0072690PECO:0000126,
PECO:0000005
single cellin situ hybridization assay evidencePMID:24161933
2.6during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.67during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancer148
Pathway
DescriptionQualifierReferenceCount
Mad2-dependent checkpoint response7
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4605
conserved in eukaryotes4515
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPCC1322.12c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byspc7NMS complex subunit, Spc105/KNL-1 family member, blinkin, Spc7 Affinity Capture-WesternPMID:22521786
affinity captured byclp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
affinity captured bypir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
affinity captured bybub3mitotic spindle checkpoint protein Bub3 Affinity Capture-WesternPMID:15525673
binds activation domain construct withmad3mitotic spindle checkpoint protein Mad3 Two-hybridPMID:23695164
binds activation domain construct withbub1mitotic spindle checkpoint kinase Bub1 Two-hybridPMID:26771498
forms complex withspc7NMS complex subunit, Spc105/KNL-1 family member, blinkin, Spc7 Reconstituted ComplexPMID:22660415
forms complex withbub3mitotic spindle checkpoint protein Bub3 Reconstituted ComplexPMID:22660415
binds DNA-binding domain construct withSPBC1685.04Schizosaccharomyces specific protein Two-hybridPMID:26771498
affinity capturesbub3mitotic spindle checkpoint protein Bub3 Affinity Capture-WesternPMID:15525673
interacts withSPBC1685.04Schizosaccharomyces specific protein PCAPMID:26771498
modified bycdc2cyclin-dependent protein kinase Cdk1/Cdc2 Biochemical ActivityPMID:12606573
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPCC1322.12c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
rescued by overexpression ofcut7kinesin family plus-end microtubule motor Cut7 Dosage RescuePMID:26258632
synthetic growth defect withcid12poly(A) polymerase Cid12 Synthetic Growth DefectPMID:11069763
synthetic growth defect withhrk1haspin related kinase Hrk1 Synthetic Growth DefectPMID:20929775
synthetic growth defect withmad2mitotic spindle checkpoint protein Mad2 Synthetic Growth DefectPMID:12606573
synthetic growth defect withpot1telomere end-binding protein Pot1 Synthetic Growth DefectPMID:24469396
synthetic growth defect withmcl1DNA polymerase alpha accessory factor Mcl1 Synthetic Growth DefectPMID:12455694
synthetic growth defect withmph1dual specificity protein kinase Mph1 Synthetic Growth DefectPMID:22281223
synthetic growth defect withsim3NASP family CENP-A chaperone Synthetic Growth DefectPMID:12719471
synthetic growth defect withrna14mRNA cleavage and polyadenylation specificity factor complex subunit Rna14 (predicted) Synthetic Growth DefectPMID:26581324
synthetic growth defect withfin1serine/threonine protein kinase, NIMA related Fin1 Synthetic Growth DefectPMID:11927555
synthetic growth defect withpfs2WD repeat protein Pfs2 Synthetic Growth DefectPMID:15743824
synthetic growth defect withmal3EB1 family Mal3 Synthetic Growth DefectPMID:16179942
synthetic growth defect withsgo2inner centromere protein, shugoshin Sgo2 Synthetic Growth DefectPMID:14730319
synthetic growth defect withswi6HP1 family chromodomain protein Swi6 Synthetic Growth DefectPMID:14730319
synthetic growth defect withrqh1RecQ type DNA helicase Rqh1 Synthetic Growth DefectPMID:24469396
synthetic growth defect withscm3CENP-A chaperone Scm3 Synthetic Growth DefectPMID:12719471
synthetic growth defect withgtb1gamma-tubulin Gtb1 Synthetic Growth DefectPMID:17277378
synthetic growth defect withtaz1human TRF ortholog Taz1 Synthetic Growth DefectPMID:12620220
overexpression causes growth defect topds5mitotic and meiotic cohesin-associated protein Pds5 Dosage Growth DefectPMID:20929775
synthetic lethal withcsi1mitotic chromosome segregation protein Csi1 Synthetic LethalityPMID:23166349
synthetic lethal withcid14poly(A) polymerase Cid14 Synthetic LethalityPMID:16478992
synthetic lethal withatb2tubulin alpha 2 Synthetic LethalityPMID:16394105
synthetic lethal withfin1serine/threonine protein kinase, NIMA related Fin1 Synthetic LethalityPMID:12065422
synthetic lethal withklp5kinesin-like protein Klp5 Synthetic LethalityPMID:11870212
synthetic lethal withklp6kinesin-like protein Klp6 Synthetic LethalityPMID:11870212
synthetic lethal withmde4microtubule-site clamp monopolin complex subunit Mde4 Synthetic LethalityPMID:19523829
synthetic lethal withswi6HP1 family chromodomain protein Swi6 Synthetic LethalityPMID:9864354
rescuespot1telomere end-binding protein Pot1 Phenotypic SuppressionPMID:24469396
rescuesrqh1RecQ type DNA helicase Rqh1 Phenotypic SuppressionPMID:24469396
rescuesalp5actin-like protein Arp4 Phenotypic SuppressionPMID:15483052
phenotype enhanced bybgs11,3-beta-glucan synthase catalytic subunit Bgs1 Phenotypic EnhancementPMID:15146064
phenotype enhanced bydad2DASH complex subunit Dad2 Phenotypic EnhancementPMID:18632983
phenotype enhanced bymph1dual specificity protein kinase Mph1 Phenotypic EnhancementPMID:22825872
phenotype enhanced byarc3ARP2/3 actin-organizing complex subunit Arc21 Phenotypic EnhancementPMID:20668161
synthetically rescuesmph1dual specificity protein kinase Mph1 Synthetic RescuePMID:22184248
External References
Database Identifier Description
NBRP SPCC1322.12c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1322.12c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1322.12c BioGRID Interaction Datasets
Expression Viewer SPCC1322.12c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1322.12c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1322.12c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1322.12c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1322.12c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1322.12c Transcriptome Viewer (Bähler Lab)
GEO SPCC1322.12c GEO profiles
PInt SPCC1322.12c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC1322.12c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC1322.12c Fission yeast phenotypic data & analysis
Cyclebase SPCC1322.12c.1 Cell Cycle Data
SPD / RIKEN30/30A12Orfeome Localization Data
UniProtKB/SwissProtO94751Checkpoint serine/threonine-protein kinase bub1
ModBaseO94751Database of comparative protein structure models
STRINGO94751Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588140serine/threonine protein kinase Bub1
RefSeq mRNANM_001023130972h- serine/threonine protein kinase Bub1 (bub1), mRNA

Literature for bub1

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016