bub1 (SPCC1322.12c)

Gene Standard Namebub1 Characterisation Statuspublished
Systematic IDSPCC1322.12c Feature Typeprotein coding
Synonyms Name Description
Productmitotic spindle checkpoint kinase Bub1 Product Size1044aa, 118.35 kDa
Genomic Location Chromosome III, 1316005-1312560 (3446nt); CDS:1315932-1312798 (3135nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
histone kinase activity (H2A-S121 specific)1
Annotation ExtensionEvidenceWith/FromReference
protein binding831
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
attachment of mitotic spindle microtubules to kinetochore34
Annotation ExtensionEvidenceWith/FromReference
cellular protein localization550
Annotation ExtensionEvidenceWith/FromReference
histone H2A-S121 phosphorylation1
Annotation ExtensionEvidenceWith/FromReference
meiotic sister chromatid cohesion, centromeric6
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle assembly checkpoint12
Annotation ExtensionEvidenceWith/FromReference
protein localization to kinetochore15
Annotation ExtensionEvidenceWith/FromReference
signal transduction involved in mitotic spindle assembly checkpoint5
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
bub1-bub3 complex2
Annotation ExtensionEvidenceWith/FromReference
condensed nuclear chromosome kinetochore59
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
old mitotic spindle pole body4
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopybub1ΔNullPMID:204732893759
Microscopynot recorded (unrecorded)PMID:11331883
Microscopybub1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000151abnormal meiotic chromosome segregationMicroscopybub1ΔNullPMID:2337039229
FYPO:0001840increased minichromosome loss during vegetative growthCell growth assaybub1ΔNullPMID:2252178634
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopybub1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
FYPO affected by mutation in bub3 mitotic spindle checkpoint protein Bub3 PMID:22521786
FYPO affected by mutation in dis3 3'-5' exoribonuclease subunit Dis3 PMID:17380189
FYPO affected by mutation in mal3 EB1 family Mal3 PMID:16179942
FYPO affected by mutation in mph1 dual specificity protein kinase Mph1 PMID:22521786
FYPO affected by mutation in msd1 mitotic-spindle disanchored Msd1 PMID:17486116
FYPO affected by mutation in spc7 kinetochore protein blinkin, Spc7 PMID:22521786
GO localized by bub3 mitotic spindle checkpoint protein Bub3 PMID:15509783
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08311 Pfam IPR013212 Mad3/BUB1 homology region 1 31 159 2
PF00069 Pfam IPR000719 Protein kinase domain 720 984 109
SM00777 SMART IPR013212 Mad3/BUB1 homology region 1 29 159 2
SM00220 SMART IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain 718 1022 107
PS50011 Prosite Profiles IPR000719 Protein kinase domain 718 1022 110
PS51489 Prosite Profiles IPR013212 Mad3/BUB1 homology region 1 36 204 2
PTHR14030:SF0 HMMPANTHER 19 1042 2
PTHR14030 HMMPANTHER IPR015661 Mitotic checkpoint serine/threonine protein kinase Bub1/Mitotic spindle checkpoint component Mad3 19 1042 2
1.10.510.10 Gene3D 858 908 112 Gene3D 716 857 111
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 717 981 127

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.37 Da
Charge -6.00
Isoelectric point 6.21
Molecular weight 118.35 kDa
Number of residues 1044

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
present during cellular response to thiabendazole
experimental evidenceS600PMID:24763107
experimental evidenceS372PMID:24763107
experimental evidenceS412PMID:24763107
experimental evidenceS415PMID:24763107
present during mitotic M phaseexperimental evidenceS323PMID:21712547
present during mitotic M phaseexperimental evidenceS381PMID:21712547
experimental evidenceS532PMID:24763107
present during mitotic M phaseexperimental evidenceS338PMID:21712547
present during mitotic M phaseexperimental evidenceS600PMID:21712547
present during mitotic M phaseexperimental evidenceS545PMID:21712547
present during mitotic M phaseexperimental evidenceS558PMID:21712547
present during mitotic M phaseexperimental evidenceS403PMID:21712547
present during mitotic M phaseexperimental evidenceS405PMID:21712547
present during mitotic M phaseexperimental evidenceS327PMID:21712547
present during mitotic M phaseexperimental evidenceS321PMID:21712547
present during mitotic M phaseexperimental evidenceS532PMID:21712547
present during mitotic M phaseexperimental evidenceS316PMID:21712547
present during mitotic M phaseexperimental evidenceS372PMID:21712547
MOD:00047O-phospho-L-threonineexperimental evidenceT342PMID:24763107692
present during mitotic M phaseexperimental evidenceT541PMID:21712547
present during mitotic M phaseexperimental evidenceT386PMID:21712547
present during mitotic M phaseexperimental evidenceT534PMID:21712547
MOD:00696phosphorylated residueNASPMID:182575171922
MOD:01455O-phosphorylated residueIDAS372PMID:232973481749
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1093during GO:0000085PECO:0000005,
single_cellexperimental evidencePMID:24161933
511.03during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
785during GO:0007067PECO:0000005,
single_cellexperimental evidencePMID:24161933
1961.71during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
9.2during GO:0072690PECO:0000005,
single_cellin situ hybridization assay evidencePMID:24161933
2.6during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
0.67during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Disease Association
Mad2-dependent checkpoint response7
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
mad3mitotic spindle checkpoint protein Mad3 Two-hybridPMID:23695164
pir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Biochemical ActivityPMID:12606573
spc7kinetochore protein blinkin, Spc7 Reconstituted ComplexPMID:22660415
Affinity Capture-WesternPMID:22521786
bub3mitotic spindle checkpoint protein Bub3 Reconstituted ComplexPMID:22660415
Affinity Capture-WesternPMID:15525673
Affinity Capture-WesternPMID:15509783
bub1mitotic spindle checkpoint kinase Bub1 Two-hybridPMID:23695164
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
taz1human TRF ortholog Taz1 Synthetic Growth DefectPMID:12620220
csi1mitotic chromosome segregation protein Csi1 Synthetic LethalityPMID:23166349
mph1dual specificity protein kinase Mph1 Phenotypic EnhancementPMID:22825872
Synthetic RescuePMID:22184248
Synthetic Growth DefectPMID:22281223
Phenotypic Enhancement
klp6kinesin-like protein Klp6 Synthetic LethalityPMID:11870212
scm3CENP-A chaperone Scm3 Synthetic Growth DefectPMID:12719471
mad2mitotic spindle checkpoint protein Mad2 Synthetic Growth DefectPMID:12606573
pot1telomere end-binding protein Pot1 Phenotypic SuppressionPMID:24469396
Synthetic Growth Defect
arc3ARP2/3 actin-organizing complex subunit Arc21 Phenotypic EnhancementPMID:20668161
cid12poly(A) polymerase Cid12 Synthetic Growth DefectPMID:16738311
mal3EB1 family Mal3 Synthetic Growth DefectPMID:16179942
cid14poly(A) polymerase Cid14 Synthetic LethalityPMID:16478992
gtb1gamma-tubulin Gtb1 Synthetic Growth DefectPMID:17277378
mde4monopolin-like complex subunit Mde4 Synthetic LethalityPMID:19523829
pds5mitotic cohesin-associated protein Pds5 Dosage Growth DefectPMID:20929775
alp5actin-like protein Arp4 Phenotypic SuppressionPMID:15483052
sim3NASP family CENP-A chaperone Synthetic Growth DefectPMID:12719471
sgo2inner centromere protein, shugoshin Sgo2 Synthetic Growth DefectPMID:14730319
dad2DASH complex subunit Dad2 Phenotypic EnhancementPMID:18632983
hrk1haspin related kinase Hrk1 Synthetic Growth DefectPMID:20929775
fin1serine/threonine protein kinase, NIMA related Fin1 Synthetic LethalityPMID:12065422
Synthetic Growth DefectPMID:11927555
mcl1DNA polymerase alpha accessory factor Mcl1 Synthetic Growth DefectPMID:12455694
rqh1RecQ type DNA helicase Rqh1 Synthetic Growth DefectPMID:24469396
Phenotypic Suppression
atb2tubulin alpha 2 Synthetic LethalityPMID:16394105
bgs11,3-beta-glucan synthase catalytic subunit Bgs1 Phenotypic EnhancementPMID:15146064
klp5kinesin-like protein Klp5 Synthetic LethalityPMID:11870212
pfs2WD repeat protein Pfs2 Synthetic Growth DefectPMID:15743824
swi6HP1 family chromodomain protein Swi6 Synthetic Growth DefectPMID:14730319
Synthetic LethalityPMID:9864354
External References
Database Identifier Description
NBRP SPCC1322.12c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1322.12c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1322.12c BioGRID Interaction Datasets
Expression Viewer SPCC1322.12c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1322.12c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1322.12c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1322.12c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1322.12c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1322.12c Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC1322.12c Cell Cycle Data
GEO SPCC1322.12c GEO profiles
PInt SPCC1322.12c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC1322.12c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC1322.12c Fission yeast phenotypic data & analysis
SPD / RIKEN30/30A12Orfeome Localization Data
UniProtKB/SwissProtO94751Checkpoint serine/threonine-protein kinase bub1
ModBaseO94751Database of comparative protein structure models
STRINGO94751Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588140serine/threonine protein kinase Bub1
RefSeq mRNANM_001023130972h- serine/threonine protein kinase Bub1 (bub1), mRNA
European Nucleotide ArchiveAAC98348.1ENA Protein Mapping
European Nucleotide ArchiveCAA22865.1ENA Protein Mapping
UniParcUPI0000126B2DUniProt Archive

Literature for bub1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014