bub1 (SPCC1322.12c)


Gene Standard Namebub1 Characterisation Statuspublished
Systematic IDSPCC1322.12c Feature Typeprotein coding
Synonyms Name Description
Productserine/threonine protein kinase Bub1 Product Size1044aa, 118.35 kDa
Genomic Location Chromosome III, 1316005-1312560 (3446nt); CDS:1315932-1312798 (3135nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037557
GO:0072371histone kinase activity (H2A-S121 specific)IDAPMID:199653871
has substrate hta1IDAPMID:19965387
has substrate hta2IDAPMID:19965387
GO:0005515protein bindingIPIbub3PMID:15525673861
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0008608attachment of spindle microtubules to kinetochoreIGImal3PMID:1639410544
GO:0051455attachment of spindle microtubules to kinetochore involved in homologous chromosome segregationIMPPMID:1996538712
GO:0034613cellular protein localization552
localizes mad3IMPPMID:15525673
localizes bub3IMPPMID:15509783
GO:0072370histone H2A-S121 phosphorylationIDAPMID:199653871
GO:0051754meiotic sister chromatid cohesion, centromericIGIsgo1PMID:147303196
GO:0007094mitotic spindle assembly checkpointIMPPMID:986435427
GO:0034501protein localization to kinetochore17
localizes sgo2IMPPMID:19965387
localizes sgo2IMPPMID:14730319
localizes sgo1IMPPMID:14730319
GO:0072480signal transduction involved in mitotic spindle assembly checkpointIMPPMID:225217865
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:1990298bub1-bub3 complexIMPPMID:225217862
GO:0000778condensed nuclear chromosome kinetochoreIDAPMID:1473031960
GO:0005634nucleus2740
predominantlyIDAPMID:15525673
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopybub1ΔNullPECO:0000005, PECO:0000137PMID:236978063730
Microscopybub1ΔNullPMID:20473289
Microscopynot recorded (unrecorded)PMID:11331883

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000151abnormal meiotic chromosome segregationMicroscopybub1ΔNullPMID:2337039229
FYPO:0001840increased minichromosome loss during vegetative growthCell growth assaybub1ΔNullPMID:2252178630
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopybub1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in bub3 mitotic spindle checkpoint protein Bub3 PMID:22521786
FYPO affected by mutation in mph1 dual specificity protein kinase Mph1 PMID:22521786
FYPO affected by mutation in spc7 kinetochore protein blinkin, Spc7 PMID:22521786
GO localized by bub3 mitotic spindle checkpoint protein Bub3 PMID:15509783
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
113160051312560

UTRs

Region Coordinates Reference
five_prime_UTR1316005..1315933PMID:21511999
three_prime_UTR1312797..1312560PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08311 Pfam IPR013212 Mad3/BUB1 homology region 1 31 159 2
PF00069 Pfam IPR000719 Protein kinase domain 720 984 109
SM00777 SMART IPR013212 Mad3/BUB1 homology region 1 29 159 2
SM00220 SMART IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain 718 1022 107
PS50011 Prosite Profiles IPR000719 Protein kinase domain 718 1022 110
PS51489 Prosite Profiles IPR013212 Mad3/BUB1 homology region 1 36 204 2
PTHR14030:SF4 HMMPANTHER 16 1040 1
PTHR14030 HMMPANTHER IPR015661 Mitotic checkpoint serine/threonine protein kinase Bub1/Mitotic spindle checkpoint component Mad3 16 1040 2
1.10.510.10 Gene3D 858 908 112
3.30.200.20 Gene3D 716 857 111
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 717 981 127

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.37 Da
Charge -6.00
Isoelectric point 6.21
Molecular weight 118.35 kDa
Number of residues 1044
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS372PMID:217125471669
present during mitotic M phase
S412PMID:24763107
present during mitotic M phaseS600PMID:21712547
present during mitotic M phaseS403PMID:21712547
present during mitotic M phaseS321PMID:21712547
present during mitotic M phaseS327PMID:21712547
present during mitotic M phaseS316PMID:21712547
present during mitotic M phaseS558PMID:21712547
present during mitotic M phaseS545PMID:21712547
present during cellular response to thiabendazoleIDAS545PMID:18257517
S415PMID:24763107
present during mitotic M phaseS405PMID:21712547
S372PMID:24763107
S600PMID:24763107
present during mitotic M phaseS381PMID:21712547
present during mitotic M phaseS323PMID:21712547
present during mitotic M phaseS532PMID:21712547
present during mitotic M phaseS338PMID:21712547
S532PMID:24763107
MOD:00047O-phospho-L-threonineT541PMID:21712547689
present during mitotic M phase
T342PMID:24763107
present during mitotic M phaseT534PMID:21712547
present during mitotic M phaseT386PMID:21712547
MOD:00696phosphorylated residueNASPMID:182575171921
NASPMID:19547744
MOD:01455O-phosphorylated residueIDAS372PMID:232973481748
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1093during GO:0000085PECO:0000126,
PECO:0000005
experimental evidencePMID:24161933
511.03during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
785during GO:0007067PECO:0000126,
PECO:0000005
experimental evidencePMID:24161933
1961.71during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2.6during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
9.2during GO:0072690PECO:0000126,
PECO:0000005
in situ hybridization assay evidencePMID:24161933
0.67during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancer142
Pathway
DescriptionQualifierReferenceCount
Mad2-dependent checkpoint response7
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
taz1human TRF ortholog Taz1 Synthetic Growth DefectPMID:12620220
csi1chromosome segregation protein Csi1 Synthetic LethalityPMID:23166349
mph1dual specificity protein kinase Mph1 Phenotypic EnhancementPMID:22825872
Synthetic RescuePMID:22184248
Phenotypic EnhancementPMID:22281223
Synthetic Growth Defect
klp6kinesin-like protein Klp6 Synthetic LethalityPMID:11870212
scm3CENP-A chaperone Scm3 Synthetic Growth DefectPMID:12719471
mad2spindle checkpoint protein Mad2 Synthetic Growth DefectPMID:12606573
arc3ARP2/3 actin-organizing complex subunit Arc21 Phenotypic EnhancementPMID:20668161
cid12poly(A) polymerase Cid12 Synthetic Growth DefectPMID:16738311
mal3EB1 family Mal3 Synthetic Growth DefectPMID:16179942
cid14poly(A) polymerase Cid14 Synthetic LethalityPMID:16478992
gtb1gamma-tubulin Gtb1 Synthetic Growth DefectPMID:17277378
mde4monopolin-like complex subunit Mde4 Synthetic LethalityPMID:19523829
pds5cohesin-associated protein Pds5 Dosage Growth DefectPMID:20929775
alp5actin-like protein Arp4 Phenotypic SuppressionPMID:15483052
sim3NASP family CENP-A chaperone Synthetic Growth DefectPMID:12719471
sgo2inner centromere protein, shugoshin Sgo2 Synthetic Growth DefectPMID:14730319
dad2DASH complex subunit Dad2 Phenotypic EnhancementPMID:18632983
hrk1haspin related kinase Hrk1 Synthetic Growth DefectPMID:20929775
fin1serine/threonine protein kinase, NIMA related Fin1 Synthetic LethalityPMID:12065422
Synthetic Growth DefectPMID:11927555
mcl1DNA polymerase alpha accessory factor Mcl1 Synthetic Growth DefectPMID:12455694
atb2tubulin alpha 2 Synthetic LethalityPMID:16394105
bgs11,3-beta-glucan synthase catalytic subunit Bgs1 Phenotypic EnhancementPMID:15146064
klp5kinesin-like protein Klp5 Synthetic LethalityPMID:11870212
pfs2WD repeat protein Pfs2 Synthetic Growth DefectPMID:15743824
swi6HP1 family chromodomain protein Swi6 Synthetic Growth DefectPMID:14730319
Synthetic LethalityPMID:9864354
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
mad3mitotic spindle checkpoint protein Mad3 Two-hybridPMID:23695164
bub3mitotic spindle checkpoint protein Bub3 Reconstituted ComplexPMID:22660415
Affinity Capture-WesternPMID:15525673
Affinity Capture-WesternPMID:15509783
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Biochemical ActivityPMID:12606573
spc7kinetochore protein blinkin, Spc7 Reconstituted ComplexPMID:22660415
Affinity Capture-WesternPMID:22521786
bub1serine/threonine protein kinase Bub1 Two-hybridPMID:23695164
External References
Database Identifier Description
NBRP SPCC1322.12c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1322.12c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1322.12c BioGRID Interaction Datasets
Expression Viewer SPCC1322.12c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1322.12c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1322.12c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1322.12c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1322.12c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1322.12c Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC1322.12c Cell Cycle Data
GEO SPCC1322.12c GEO profiles
PInt SPCC1322.12c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC1322.12c Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN30/30A12Orfeome Localization Data
WikiGene2538736serine/threonine protein kinase Bub1
EntrezGene2538736serine/threonine protein kinase Bub1
UniProtKB/SwissProtO94751Checkpoint serine/threonine-protein kinase bub1
ModBaseO94751Database of comparative protein structure models
STRINGO94751Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588140serine/threonine protein kinase Bub1
RefSeq mRNANM_001023130972h- serine/threonine protein kinase Bub1 (bub1), mRNA
European Nucleotide ArchiveAAC98348ENA Protein Mapping
European Nucleotide ArchiveAAC98348.1ENA Protein Mapping
European Nucleotide ArchiveCAA22865ENA Protein Mapping
European Nucleotide ArchiveCAA22865.1ENA Protein Mapping
UniParcUPI0000126B2DUniProt Archive

Literature for bub1

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014