bdf1 (SPCC1450.02)


Gene Standard Namebdf1 Characterisation Statuspublished
Systematic IDSPCC1450.02 Feature Typeprotein coding
SynonymsSPCC191.13 Name Description
ProductSwr1 complex bromodomain subunit Bdf1 Product Size578aa, 65.19 kDa
Genomic Location Chromosome III, 1728388-1731295 (2908nt); CDS:1728861-1730597 (1737nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
chromatin remodeling156
Annotation ExtensionEvidenceWith/FromReference
histone exchange23
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
Swr1 complex15
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
sensitive to 4-nitroquinoline N-oxidebdf1Δ159
sensitive to 5-fluorouracilbdf1Δ273
viable vegetative cell populationbdf1Δ3837

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologybdf1Δ3100
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1728388..1731295
5' UTR1728388..1728860PMID:21511999
3' UTR1730598..1731295PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00439 Pfam IPR001487 Bromodomain 93 172 9
PF00439 Pfam IPR001487 Bromodomain 260 345 9
PF17035 Pfam IPR027353 NET domain 439 501 2
SM00297 SMART IPR001487 Bromodomain 86 192 9
SM00297 SMART IPR001487 Bromodomain 253 362 9
PS51525 Prosite Profiles IPR027353 NET domain 430 510 2
PS50014 Prosite Profiles IPR001487 Bromodomain 101 173 9
PS50014 Prosite Profiles IPR001487 Bromodomain 271 343 9
PS00633 Prosite Patterns IPR018359 Bromodomain, conserved site 276 335 7
PTHR22880:SF5 HMMPANTHER 63 500 2
PTHR22880:SF5 HMMPANTHER 519 577 2
PTHR22880 HMMPANTHER 63 500 4
PTHR22880 HMMPANTHER 519 577 4
1.20.920.10 Gene3D IPR001487 Bromodomain 235 359 10
1.20.920.10 Gene3D IPR001487 Bromodomain 69 209 10
SSF47370 SuperFamily IPR001487 Bromodomain 239 364 10
SSF47370 SuperFamily IPR001487 Bromodomain 74 194 10
PR00503 PRINTS IPR001487 Bromodomain 324 343 9
PR00503 PRINTS IPR001487 Bromodomain 306 324 9
PR00503 PRINTS IPR001487 Bromodomain 274 287 9
PR00503 PRINTS IPR001487 Bromodomain 290 306 9

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000496bromodomainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000496

Protein Properties

Ave. residue weight 112.79 Da
Charge -12.50
Codon Adaptation Index 0.46
Isoelectric point 4.92
Molecular weight 65.19 kDa
Number of residues 578
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phaseS34
present during mitotic M phaseS239
present during mitotic M phaseS528
present during mitotic M phaseS391
present during mitotic M phaseS35
present during mitotic M phaseS23
present during mitotic M phaseS52
S239, S391, S528
level fluctuates during mitotic cell cycleS52
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S34 PMID:21712547
present during mitotic M phase experimental evidence S239 PMID:21712547
present during mitotic M phase experimental evidence S528 PMID:21712547
present during mitotic M phase experimental evidence S391 PMID:21712547
present during mitotic M phase experimental evidence S35 PMID:21712547
present during mitotic M phase experimental evidence S23 PMID:21712547
present during mitotic M phase experimental evidence S52 PMID:21712547
experimental evidence S528 PMID:24763107
level fluctuates during mitotic cell cycle experimental evidence S52 PMID:24763107
experimental evidence S239 PMID:24763107
experimental evidence S391 PMID:24763107
O-phospho-L-threonine 700
present during mitotic M phaseT14
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T14 PMID:21712547
phosphorylated residue 1930
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for bdf1 (SPCC1450.02)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
12408during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
12598during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
12066during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
11545during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5189.31during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
12047during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3939during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
12during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4605
conserved in eukaryotes4515
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPCC1450.02 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byepe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
affinity captured bybdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
binds activation domain construct withmug135mug2/mug135/meu2 family, with repeat expansion Two-hybridPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPCC1450.02 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetically rescueschk1Chk1 protein kinase Synthetic RescuePMID:22095079
synthetically rescuescrb2DNA repair protein Rad9 homolog Crb2 Synthetic RescuePMID:22095079
synthetically rescuesswi1replication fork protection complex subunit Swi1 Synthetic RescuePMID:22095079
synthetically rescuescds1replication checkpoint kinase Cds1 Synthetic RescuePMID:22095079
synthetically rescuesmrc1mediator of replication checkpoint 1 Synthetic RescuePMID:22095079
negative genetic interaction withSPAPB1E7.11cSchizosaccharomyces specific protein Negative GeneticPMID:22681890
negative genetic interaction withSPCC126.08clectin family glycoprotein receptor (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcbp1CENP-B homolog Negative GeneticPMID:18818364
negative genetic interaction withask1DASH complex subunit Ask1 Negative GeneticPMID:18818364
negative genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withmcl1DNA polymerase alpha accessory factor Mcl1 Negative GeneticPMID:22681890
negative genetic interaction withapq12nuclear membrane organization protein Apq12 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmug154conserved fungal protein Negative GeneticPMID:18818364
negative genetic interaction withmug123Schizosaccharomyces specific protein Mug123 Negative GeneticPMID:22681890
negative genetic interaction withhip1hira protein, histone chaperone Hip1 Negative GeneticPMID:18818364
negative genetic interaction withuri1unconventional prefoldin chaperone involved protein complex assembly Uri1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsds23PP2A-type phosphatase inhibitor Sds23/Moc1 Negative GeneticPMID:22681890
negative genetic interaction withnem1Nem1-Spo7 phosphatase complex catalytic subunit Nem1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withtom70mitochondrial TOM complex subunit Tom70 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcay1cactin, spliceosome complex subunit Negative GeneticPMID:18818364
negative genetic interaction withhrp1ATP-dependent DNA helicase Hrp1 Negative GeneticPMID:18818364
negative genetic interaction withshf1small histone ubiquitination factor Shf1 Negative GeneticPMID:22681890
negative genetic interaction withSPAC17H9.13cglutamate 5-kinase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
negative genetic interaction withSPBC31F10.10czf-MYND type zinc finger protein Negative GeneticPMID:18818364
negative genetic interaction witharv1Arv1-like family protein Arv1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withbdc1bromodomain containing protein 1, Bdc1 Negative GeneticPMID:22681890
negative genetic interaction withrpl270160S ribosomal protein L27 Negative GeneticPMID:22681890
negative genetic interaction withbdf2BET family double bromodomain protein Bdf2 Negative GeneticPMID:18818364
negative genetic interaction withfar10SIP/FAR complex FHA domain subunit Far10/Csc1 Negative GeneticPMID:22681890
negative genetic interaction withemc1ER membrane protein complex subunit Emc1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction witharp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:18818364
negative genetic interaction withser2phosphoserine phosphatase Ser2 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withrps40140S ribosomal protein S4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withdad2DASH complex subunit Dad2 Negative GeneticPMID:22681890
negative genetic interaction withSPAC19B12.11czinc finger protein, human ZNF593 ortholog Negative GeneticPMID:18818364
negative genetic interaction withfkbp39FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) Negative GeneticPMID:18818364
negative genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:18818364
negative genetic interaction withsat1Golgi membrane exchange factor subunit Sat1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withngg1SAGA complex subunit Ngg1 Negative GeneticPMID:18818364
negative genetic interaction withcaa1cytoplasmic aspartate aminotransferase Caa1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpnk1DNA kinase/phosphatase Pnk1 Negative GeneticPMID:18818364
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:18818364
negative genetic interaction withSPBC13E7.03cRNA hairpin binding protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withspo20sec14 cytosolic factor family, glycerophospholipid-transfer protein Spo20/Sec14 Negative GeneticPMID:22681890
negative genetic interaction withrad57RecA family ATPase Rad57/Rhp57 Negative GeneticPMID:22681890
negative genetic interaction withpob3FACT complex subunit Pob3 Negative GeneticPMID:18818364
negative genetic interaction withmgr2TIM23 translocase complex subunit Mgr2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsnz1pyridoxine biosynthesis protein Negative GeneticPMID:22681890
negative genetic interaction withski2Ski complex RNA helicase Ski2 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withmca1transcription factor, zf-fungal binuclear cluster type Mca1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withclp1Cdc14-related protein phosphatase Clp1/Flp1 Negative GeneticPMID:22681890
negative genetic interaction withnop52nucleolar protein Nop52 family Rrp1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withalp31tubulin specific chaperone cofactor A, Alp31 Negative GeneticPMID:18818364
negative genetic interaction withSPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:18818364
negative genetic interaction withyaf9YEATS family histone acetyltransferase subunit Yaf9 Negative GeneticPMID:18818364
negative genetic interaction witherp2COPII-coated vesicle component Erp2/3/4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withspc34DASH complex subunit Spc34 Negative GeneticPMID:18818364
negative genetic interaction withcho1phosphatidyl-N-dimethylethanolamine N-methyltransferase Negative GeneticPMID:18818364
negative genetic interaction withubp8SAGA complex ubiquitin C-terminal hydrolase Ubp8 Negative GeneticPMID:18818364
positive genetic interaction withSPBC25H2.15SSU-rRNA maturation protein Tsr4 homolog 1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withsec28coatomer epsilon subunit (predicted) Positive GeneticPMID:18818364
positive genetic interaction withtcg1single-stranded telomeric binding protein Tgc1 Positive GeneticPMID:22681890
positive genetic interaction withpdp1PWWP domain protein Pdp1 Positive GeneticPMID:18818364
positive genetic interaction withelp3elongator complex subunit Elp3 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withrsc1RSC complex subunit Rsc1 Positive GeneticPMID:18818364
positive genetic interaction withrsc4RSC complex subunit Rsc4 Positive GeneticPMID:18818364
positive genetic interaction withxks1xylulose kinase Xks1 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withmug20Schizosaccharomyces specific protein Mug20 Positive GeneticPMID:22681890
rescuesmrc1mediator of replication checkpoint 1 Phenotypic SuppressionPMID:22095079
enhances phenotype ofalp13MRG family Clr6 histone deacetylase complex subunit Alp13 Phenotypic EnhancementPMID:19547744
synthetic growth defect withchk1Chk1 protein kinase Synthetic Growth DefectPMID:22095079
synthetic growth defect withmrc1mediator of replication checkpoint 1 Synthetic Growth DefectPMID:22095079
synthetic growth defect withrad3ATR checkpoint kinase Rad3 Synthetic Growth DefectPMID:22095079
synthetic growth defect withcds1replication checkpoint kinase Cds1 Synthetic Growth DefectPMID:22095079
synthetic lethal withbdf2BET family double bromodomain protein Bdf2 Synthetic LethalityPMID:22095079
External References
Database Identifier Description
NBRP SPCC1450.02 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1450.02 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1450.02 BioGRID Interaction Datasets
Expression Viewer SPCC1450.02 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1450.02 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1450.02 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1450.02 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1450.02 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1450.02 Transcriptome Viewer (Bähler Lab)
GEO SPCC1450.02 GEO profiles
PInt SPCC1450.02 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC1450.02 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC1450.02 Fission yeast phenotypic data & analysis
Cyclebase SPCC1450.02.1 Cell Cycle Data
SPD / RIKEN35/35H03Orfeome Localization Data
UniProtKB/SwissProtQ9Y7N0SWR1 complex bromodomain subunit bdf1
ModBaseQ9Y7N0Database of comparative protein structure models
STRINGQ9Y7N0Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588301Swr1 complex bromodomain subunit Brf1
RefSeq mRNANM_001023291972h- Swr1 complex bromodomain subunit Brf1 (bdf1), mRNA

Literature for bdf1

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016