adn3 (SPCC1494.10)


Gene Standard Nameadn3 Characterisation Statusbiological_role_inferred
Systematic IDSPCC1494.10 Feature Typeprotein coding
SynonymsSPCC70.01 Name DescriptionADhesioN defective
Producttranscription factor (predicted) Product Size964aa, 104.59 kDa
Genomic Location Chromosome III, 2344694-2348598 (3905nt); CDS:2345367-2348261 (2895nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000978RNA polymerase II core promoter proximal region sequence-specific DNA bindingISOSGD:S000000911GO_REF:000002494
GO:0000982RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activityISOSGD:S000000911GO_REF:000002491
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0010811positive regulation of cell-substrate adhesionIMPPMID:195423127
GO:0045944positive regulation of transcription from RNA polymerase II promoterISOSGD:S000000911GO_REF:000002481
GO:0006366transcription from RNA polymerase II promoterISOSGD:S000000911GO_REF:0000024139
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032153cell division siteIDAPMID:16823372298
GO:0005634nucleusISOSGD:S000000911GO_REF:00000242737
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000096sensitive to cadmium246
expressivity FYPO_EXT:0000002Cell growth assayadn3ΔNullPECO:0000102, PECO:0000005, PECO:0000137PMID:18684775
FYPO:0002060viable vegetative cell populationMicroscopyadn3ΔNullPMID:204732893751
Microscopyadn3ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000010abolished cell-substrate adhesionadn3ΔNullPMID:195423129
FYPO:0000551pseudohyphal growth abolishedMicroscopyadn3ΔNullPMID:1954231212
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopyadn3ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
123446942348598
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08513 Pfam IPR013720 LisH dimerisation motif, subgroup 36 62 8
SM00667 SMART IPR006594 LisH dimerisation motif 34 66 10
PS50896 Prosite Profiles IPR006594 LisH dimerisation motif 34 66 11
PTHR12610 HMMPANTHER 35 205 2
PTHR12610:SF12 HMMPANTHER 35 205 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.50 Da
Charge 8.50
Isoelectric point 7.45
Molecular weight 104.59 kDa
Number of residues 964
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS725PMID:217125471670
present during mitotic M phase
present during mitotic M phaseS723PMID:21712547
S84PMID:24763107
present during mitotic M phaseS100PMID:21712547
present during mitotic M phaseS95PMID:21712547
present during mitotic M phaseS114PMID:21712547
present during mitotic M phaseS117PMID:21712547
S727PMID:24763107
present during mitotic M phaseS834PMID:21712547
present during mitotic M phaseS840PMID:21712547
present during mitotic M phaseS727PMID:21712547
present during mitotic M phaseS113PMID:21712547
S117PMID:24763107
level fluctuates during mitotic cell cycleS840PMID:24763107
level fluctuates during mitotic cell cycleS725PMID:24763107
present during mitotic M phaseS836PMID:21712547
present during mitotic M phaseS731PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2737during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2449during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2038.21during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
2693during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
4146.82during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.6during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi only620
conserved in fungi4599
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
cdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
dad1DASH complex subunit Dad1 Negative GeneticPMID:22681890
bdc1bromodomain containing protein 1, Bdc1 Negative GeneticPMID:22681890
clr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:22681890
usp102U1 snRNP-associated protein Usp102 Positive GeneticPMID:22681890
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Negative GeneticPMID:22681890
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:22681890
not3CCR4-Not complex subunit Not3/5 (predicted) Negative GeneticPMID:22681890
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
tom70mitochondrial TOM complex subunit Tom70 (predicted) Negative GeneticPMID:22681890
raf2Rik1-associated factor Raf2 Negative GeneticPMID:22681890
snf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
gsf2galactose-specific flocculin Gsf2 Phenotypic SuppressionPMID:23236291
SPBC16H5.13WD repeat protein, human WDR7 ortholog Negative GeneticPMID:22681890
atg6beclin family protein, involved in autophagy Positive GeneticPMID:22681890
SPBC106.13ubiquitin ligase complex subunit, involved in proteasome-dependent catabolite inactivation of FBPase (predicted) Negative GeneticPMID:22681890
kin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
sap10splicing factor 3B Negative GeneticPMID:22681890
atp11F1-ATPase chaperone Atp11 (predicted) Negative GeneticPMID:22681890
slx4structure-specific endonuclease subunit Slx4 Positive GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
ypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
arp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
sts5RNB-like protein Negative GeneticPMID:22681890
hip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
tsc1hamartin Negative GeneticPMID:22681890
rpb7DNA-directed RNA polymerase complex II subunit Rpb7 Negative GeneticPMID:22681890
reb1RNA polymerase I transcription termination factor Reb1 Negative GeneticPMID:22681890
raf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Negative GeneticPMID:22681890
SPCC162.11curidine kinase/uracil phosphoribosyltransferase (predicted) Positive GeneticPMID:22681890
prw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
bun107WD repeat protein, human WDR48 family Bun107 Negative GeneticPMID:22681890
SPAC144.17c6-phosphofructo-2-kinase (predicted) Negative GeneticPMID:22681890
ddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
sap114U2 snRNP subunit Sap114 Positive GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Positive GeneticPMID:22681890
SPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
tsc2tuberin Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPCC1494.10 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1494.10 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1494.10 BioGRID Interaction Datasets
Expression Viewer SPCC1494.10 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1494.10 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1494.10 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1494.10 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1494.10 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1494.10 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC1494.10 Cell Cycle Data
GEO SPCC1494.10 GEO profiles
PInt SPCC1494.10 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC1494.10 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN47/47D07Orfeome Localization Data
UniProtKB/SwissProtO74522Adhesion defective protein 3
ModBaseO74522Database of comparative protein structure models
STRINGO74522Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588535transcription factor (predicted)
RefSeq mRNANM_001023523972h- transcription factor (predicted) (adn3), mRNA
European Nucleotide ArchiveBAA87256.1ENA Protein Mapping
European Nucleotide ArchiveCAA19308.1ENA Protein Mapping
UniParcUPI000006C3F3UniProt Archive

Literature for adn3

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014