ppk33 (SPCC162.10)

Gene Standard Nameppk33 Characterisation Statuspublished
Systematic IDSPCC162.10 Feature Typeprotein coding
Synonyms Name Description
Productserine/threonine protein kinase Ppk33 (predicted) Product Size338aa, 38.67 kDa
Genomic Location Chromosome III, 1561207-1557811 (3397nt); CDS:1559240-1558224 (1017nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding560
Annotation ExtensionEvidenceWith/FromReference
protein serine/threonine kinase activity105
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms


Term NameCount
protein phosphorylation134
Annotation ExtensionEvidenceWith/FromReference
signal transduction361
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
normal vegetative cell population growthppk33Δ851
resistance to tamoxifenppk33Δ61
sensitive to bortezomibppk33Δ255
sensitive to camptothecinppk33Δ248
sensitive to clotrimazoleppk33Δ107
sensitive to terbinafineppk33Δ103
viable vegetative cell populationppk33Δ3844

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologyppk33Δ3100
Target Of
FYPO affected by mutation in klf1 transcription factor, zf-fungal binuclear cluster type Klf1
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR1561207..1559241PMID:21511999
3' UTR1558223..1557811PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 31 302 108
SM00220 SMART IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain 30 302 107
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 147 159 97
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 36 59 73
PS50011 Prosite Profiles IPR000719 Protein kinase domain 30 302 110
PTHR24356:SF0 HMMPANTHER 26 335 1
PTHR24356 HMMPANTHER 26 335 13 Gene3D Serine/threonine-protein kinase Rio2 25 95 111
1.10.510.10 Gene3D Serine/threonine-protein kinase Rio2 96 312 112
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 24 323 127

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.41 Da
Charge 21.00
Codon Adaptation Index 0.44
Isoelectric point 9.99
Molecular weight 38.67 kDa
Number of residues 338
Gene Expression

Qualitative Gene Expression

RNA levelincreased during GO:0071456expression microarray evidencePMID:22235339

Quantitative Gene Expression

View graphical display of gene expression data for ppk33 (SPCC162.10)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.23during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
0.077during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
no apparent S. cerevisiae ortholog601
conserved in fungi4606
conserved in eukaryotes4516
conserved in eukaryotes only2505

Orthologs in Compara

Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPCC162.10 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withubp2ubiquitin C-terminal hydrolase Ubp2 Negative GeneticPMID:22681890
negative genetic interaction withclr1SHREC complex subunit Clr1 Negative GeneticPMID:22681890
negative genetic interaction witheso1sister chromatid cohesion protein/DNA polymerase eta Eso1 fusion protein Negative GeneticPMID:23050226
negative genetic interaction withtif38translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
negative genetic interaction withpyp1tyrosine phosphatase Pyp1 Negative GeneticPMID:22681890
negative genetic interaction withrga2Rho-type GTPase activating protein Rga2 Negative GeneticPMID:22681890
negative genetic interaction withsnf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
negative genetic interaction withypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
negative genetic interaction withmkh1MEK kinase (MEKK) Mkh1 Negative GeneticPMID:22681890
negative genetic interaction withcds1replication checkpoint kinase Cds1 Negative GeneticPMID:22681890
positive genetic interaction withppk16serine/threonine protein kinase Ppk16 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withres1MBF transcription factor complex subunit Res1 Positive GeneticPMID:22681890
positive genetic interaction withmph1dual specificity protein kinase Mph1 Positive GeneticPMID:22681890
positive genetic interaction withatb2tubulin alpha 2 Positive GeneticPMID:22681890
positive genetic interaction withslx4structure-specific endonuclease subunit Slx4 Positive GeneticPMID:22681890
positive genetic interaction withusp102U1 snRNP-associated protein Usp102 Positive GeneticPMID:22681890
positive genetic interaction withufd2ubiquitin-protein ligase E4 Ufd2 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withtas3RITS complex subunit 3 Positive GeneticPMID:22681890
positive genetic interaction withSPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPCC162.10 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC162.10 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC162.10 BioGRID Interaction Datasets
Expression Viewer SPCC162.10 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC162.10 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC162.10 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC162.10 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC162.10 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC162.10 Transcriptome Viewer (Bähler Lab)
GEO SPCC162.10 GEO profiles
PInt SPCC162.10 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC162.10 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC162.10 Fission yeast phenotypic data & analysis
Cyclebase SPCC162.10.1 Cell Cycle Data
SPD / RIKEN15/15B09Orfeome Localization Data
UniProtKB/SwissProtO74426Serine/threonine-protein kinase ppk33
ModBaseO74426Database of comparative protein structure models
STRINGO74426Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588234serine/threonine protein kinase Ppk33 (predicted)
RefSeq mRNANM_001023224972h- serine/threonine protein kinase Ppk33 (predicted) (ppk33), mRNA
European Nucleotide ArchiveCAA19590.1ENA Protein Mapping
UniParcUPI0000069FF8UniProt Archive

Literature for ppk33

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016