ppk33 (SPCC162.10)


Gene Standard Nameppk33 Characterisation Statuspublished
Systematic IDSPCC162.10 Feature Typeprotein coding
Synonyms Name Description
Productserine/threonine protein kinase Ppk33 (predicted) Product Size338aa, 38.67 kDa
Genomic Location Chromosome III, 1561207-1557811 (3397nt); CDS:1559240-1558224 (1017nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037557
GO:0004674protein serine/threonine kinase activityIEAUniProtKB-KW:KW-0723GO_REF:0000037112
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006468protein phosphorylationISMPFAM:PF00069GO_REF:000000146
GO:0007165signal transductionNASGO_REF:0000051307
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001357normal vegetative cell population growthCell growth assayppk33ΔNullPMID:16615890562
FYPO:0001701sensitive to bortezomib256
expressivity FYPO_EXT:0000003Cell growth assayppk33ΔNullPECO:0000005, PECO:0000137PMID:21760946
FYPO:0000085sensitive to camptothecin211
expressivity FYPO_EXT:0000003Cell growth assayppk33ΔNullPECO:0000137, PECO:0000005PMID:19264558
competitive growth assay evidenceppk33ΔNullPMID:20537132
FYPO:0002640sensitive to clotrimazole105
expressivity FYPO_EXT:0000002Cell growth assayppk33ΔNullPECO:0000137, PECO:0000005PMID:22252817
expressivity FYPO_EXT:0000003Cell growth assayppk33ΔNullPECO:0000137, PECO:0000005PMID:22252817
FYPO:0002328sensitive to terbinafine101
expressivity FYPO_EXT:0000002Cell growth assayppk33ΔNullPECO:0000137, PECO:0000005PMID:22252817
expressivity FYPO_EXT:0000003Cell growth assayppk33ΔNullPECO:0000137, PECO:0000005, PECO:0000025PMID:22252817
FYPO:0002060viable vegetative cell populationMicroscopyppk33ΔNullPMID:204732893730
Microscopyppk33ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopyppk33ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in klf1 transcription factor, zf-fungal binuclear cluster type Klf1 PMID:24167631
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
115612071557811

UTRs

Region Coordinates Reference
five_prime_UTR1561207..1559241PMID:21511999
three_prime_UTR1558223..1557811PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 31 302 109
SM00220 SMART IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain 30 302 107
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 147 159 97
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 36 59 73
PS50011 Prosite Profiles IPR000719 Protein kinase domain 30 302 110
PTHR24356 HMMPANTHER 1 335 6
PTHR24356:SF0 HMMPANTHER 1 335 1
1.10.510.10 Gene3D 96 312 112
3.30.200.20 Gene3D 25 95 111
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 24 323 127

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.41 Da
Charge 21.00
Isoelectric point 9.99
Molecular weight 38.67 kDa
Number of residues 338
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
NDduring GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.23during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
0.077during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog579
conserved in fungi4569
conserved in eukaryotes4482
conserved in eukaryotes only2487
Orthologs

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
clr1cryptic loci regulator Clr1 Negative GeneticPMID:22681890
ppk16serine/threonine protein kinase Ppk16 (predicted) Positive GeneticPMID:22681890
SPAC821.05translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
mph1dual specificity protein kinase Mph1 Positive GeneticPMID:22681890
slx4structure-specific endonuclease subunit Slx4 Positive GeneticPMID:22681890
rga2Rho-type GTPase activating protein Rga2 Negative GeneticPMID:22681890
ypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
snf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
SPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
cds1replication checkpoint kinase Cds1 Negative GeneticPMID:22681890
pyp1tyrosine phosphatase Pyp1 Negative GeneticPMID:22681890
mkh1MEK kinase (MEKK) Mkh1 Negative GeneticPMID:22681890
ubp2ubiquitin C-terminal hydrolase Ubp2 Negative GeneticPMID:22681890
usp102U1 snRNP-associated protein Usp102 Positive GeneticPMID:22681890
eso1sister chromatid cohesion protein/DNA polymerase eta Eso1 fusion protein Negative GeneticPMID:23050226
atb2tubulin alpha 2 Positive GeneticPMID:22681890
res1MBF transcription factor complex subunit Res1 Positive GeneticPMID:22681890
tas3RITS complex subunit 3 Positive GeneticPMID:22681890
ufd2ubiquitin-protein ligase E4 (predicted) Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPCC162.10 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC162.10 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC162.10 BioGRID Interaction Datasets
Expression Viewer SPCC162.10 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC162.10 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC162.10 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC162.10 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC162.10 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC162.10 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC162.10 Cell Cycle Data
GEO SPCC162.10 GEO profiles
PInt SPCC162.10 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC162.10 Peptides identified in tandem mass spectrometry proteomics experiments
WikiGene2539202serine/threonine protein kinase Ppk33 (predicted)
EntrezGene2539202serine/threonine protein kinase Ppk33 (predicted)
SPD / RIKEN15/15B09Orfeome Localization Data
UniProtKB/SwissProtO74426Serine/threonine-protein kinase ppk33
ModBaseO74426Database of comparative protein structure models
STRINGO74426Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588234serine/threonine protein kinase Ppk33 (predicted)
RefSeq mRNANM_001023224972h- serine/threonine protein kinase Ppk33 (predicted) (ppk33), mRNA
European Nucleotide ArchiveCAA19590ENA Protein Mapping
European Nucleotide ArchiveCAA19590.1ENA Protein Mapping
UniParcUPI0000069FF8UniProt Archive

Literature for ppk33

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014