Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPCC16C4.22 Feature Typeprotein coding
SynonymsSPNCRNA.470, SPNG2185 Name Description
ProductDNA polymerase epsilon Dpb3 (predicted) Product Size87aa, 10.11 kDa
Genomic Location Chromosome III, 684656-685496 (841nt); CDS:684828-685192 (365nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
sequence-specific DNA binding136
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

DNA replication

Term NameCount
mitotic DNA replication74
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
epsilon DNA polymerase complex4
Annotation ExtensionEvidenceWith/FromReference
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons684656..684947, 685049..685496
5' UTR684656..684827PMID:18488015
CDS684828..684947, 685049..685192PMID:18488015
3' UTR685193..685496
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00808 Pfam IPR003958 Transcription factor CBF/NF-Y/archaeal histone 10 71 7
PTHR10252:SF25 HMMPANTHER 2 83 1 Gene3D IPR009072 Histone-fold 10 84 27
SSF47113 SuperFamily IPR009072 Histone-fold 6 86 26

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000297histone foldTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000297

Protein Properties

Ave. residue weight 116.24 Da
Charge 1.50
Isoelectric point 8.45
Molecular weight 10.11 kDa
Number of residues 87
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8025during GO:0000080PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
7047during GO:0000084PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
4951during GO:0000085PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
5013during GO:0000087PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
5808during GO:0072690PECO:0000126,
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.1during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
0.34during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633
previously annotated as ncRNA21
dpb3 was previously considered to be SPAC17G8.03c but this new gene product appears to be the true ortholog of DPB3/POLE4 see TreeFam:TF1030091
Species Distribution
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bymtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
affinity captured bypir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
External References
Database Identifier Description
NBRP SPCC16C4.22 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC16C4.22 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC16C4.22 BioGRID Interaction Datasets
Expression Viewer SPCC16C4.22 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC16C4.22 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC16C4.22 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC16C4.22 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC16C4.22 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC16C4.22 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC16C4.22 Cell Cycle Data
GEO SPCC16C4.22 GEO profiles
PInt SPCC16C4.22 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC16C4.22 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC16C4.22 Fission yeast phenotypic data & analysis
UniProtKB/SwissProtC6Y4D0Putative transcription factor C16C4.22
ModBaseC6Y4D0Database of comparative protein structure models
STRINGC6Y4D0Network display of known and predicted interactions and functional associations
RefSeq mRNAXM_002788903972h- histone-like transcription factor family (CBF/NF-Y) (SPCC16C4.22), mRNA
RefSeq PeptideXP_002788949histone-like transcription factor family (CBF/NF-Y)
European Nucleotide ArchiveCBA11517.1ENA Protein Mapping
UniParcUPI0001B0626DUniProt Archive

Literature for SPCC16C4.22

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015