mug33 (SPCC1739.10)


Gene Standard Namemug33 Characterisation Statuspublished
Systematic IDSPCC1739.10 Feature Typeprotein coding
Synonyms Name DescriptionMeiotically Upregulated Gene
ProductTea1-interacting protein involved in exocytosis Product Size336aa, 37.07 kDa
Genomic Location Chromosome III, 2047508-2049286 (1779nt); CDS:2047936-2048946 (1011nt)

Ensembl Gene Location
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0007127meiosis IIEPPMID:1216175382
GO:0045921positive regulation of exocytosisIGIfor3PMID:216526302
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032153cell division siteIDAPMID:16823372295
during M phaseIDAPMID:21652630
GO:0051286cell tipIDAPMID:16823372188
GO:0005737cytoplasmIDAPMID:168233724201
GO:0030139endocytic vesicleIDAPMID:216526307
GO:0070382exocytic vesicleIDAPMID:216526301
GO:0035838growing cell tip19
during mitotic interphaseIMPPMID:21652630
GO:0005886plasma membraneISOSGD:S000005379GO_REF:0000024242
GO:0031520plasma membrane of cell tip10
during mitotic interphaseIDAPMID:21652630
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001128decreased septation indexMicroscopymug33ΔNullPMID:216526309
FYPO:0000674normal cell population growth at high temperatureCell growth assaymug33ΔNullPECO:0000137PMID:2165263031
FYPO:0001164normal growth on glucose carbon sourceCell growth assaymug33ΔNullPECO:0000137, PECO:0000005PMID:21652630145
FYPO:0000268sensitive to UV during vegetative growthcompetitive growth assay evidencemug33ΔNullPMID:20537132117
FYPO:0001701sensitive to bortezomib256
expressivity FYPO_EXT:0000001Cell growth assaymug33ΔNullPECO:0000005, PECO:0000137PMID:21760946
FYPO:0000085sensitive to camptothecincompetitive growth assay evidencemug33ΔNullPMID:20537132211
FYPO:0002060viable vegetative cell populationMicroscopymug33ΔNullPECO:0000005, PECO:0000137PMID:236978063760
Microscopymug33ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002848branched cell during recovery from stationary phaseMicroscopymug33ΔNullPECO:0000235, PECO:0000137PMID:216526302
FYPO:0002849curved cell during recovery from stationary phaseMicroscopymug33ΔNullPECO:0000235, PECO:0000137PMID:216526302
FYPO:0002850normal cell morphology during recovery from stationary phaseMicroscopymug33ΔNullPECO:0000137PMID:216526301
FYPO:0000426normal endocytosisOthermug33ΔNullPMID:216526302
FYPO:0002442normal protein localization to cell division site6
affecting sec6Microscopymug33ΔNullPMID:21652630
affecting sec8Microscopymug33ΔNullPMID:21652630
affecting sec10Microscopymug33ΔNullPMID:21652630
FYPO:0001587normal protein localization to cell tip14
affecting sec10Microscopymug33ΔNullPMID:21652630
affecting sec8Microscopymug33ΔNullPMID:21652630
affecting sec6Microscopymug33ΔNullPMID:21652630
FYPO:0001945normal protein secretionEnzyme assay datamug33ΔNullPMID:216526303
FYPO:0002851protein mislocalized to eisosome1
affecting mug33MicroscopyΔC-terminus (181-336)Not specifiedPMID:21652630
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopymug33ΔNullPMID:216526303086
penetrance FYPO_EXT:0000001Microscopymug33ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in end4 Huntingtin-interacting protein homolog PMID:21652630
FYPO affected by mutation in for3 formin For3 PMID:21652630
FYPO affected by mutation in mug33 Tea1-interacting protein involved in exocytosis PMID:21652630
FYPO affected by mutation in myo52 myosin type V PMID:21652630
FYPO affected by mutation in rho3 Rho family GTPase Rho3 PMID:21652630
FYPO affected by mutation in sec8 exocyst complex subunit Sec8 PMID:21652630
FYPO affected by mutation in tea1 cell end marker Tea1 PMID:21652630
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
120475082049286

UTRs

Region Coordinates Reference
five_prime_UTR2047508..2047935PMID:21511999
three_prime_UTR2048947..2049286PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF06687 Pfam IPR009571 Actin cortical patch SUR7/pH-response regulator PalI 6 165 3
TMhelix TMHMM 118 140 959
TMhelix TMHMM 9 31 959
TMhelix TMHMM 89 111 959
TMhelix TMHMM 150 172 959
SignalP-TM signalp 1 23 16

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0000418signal_peptide278
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 110.34 Da
Charge 17.00
Isoelectric point 10.15
Molecular weight 37.07 kDa
Number of residues 336
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS316PMID:217125471663
present during mitotic M phase
present during mitotic M phaseS252PMID:21712547
present during mitotic M phaseS233PMID:21712547
present during mitotic M phaseS257PMID:21712547
present during mitotic M phaseS255PMID:21712547
S233PMID:24763107
level fluctuates during mitotic cell cycleS308PMID:24763107
MOD:00696phosphorylated residueNASPMID:195477441915
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0051321experimental evidencePMID:16303567

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
25998during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
23073during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
25366during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
25339during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
23405during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
4481.14during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
653.94during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
9.5during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
4.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi only603
conserved in fungi4569
conserved in eukaryotes4482
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
slm1cytoskeletal signaling protein Slm1 (predicted) Negative GeneticPMID:22681890
taz1human TRF ortholog Taz1 Negative GeneticPMID:22681890
srk1MAPK-activated protein kinase Srk1 Negative GeneticPMID:22681890
cwf19complexed with Cdc5 protein Cwf19 Negative GeneticPMID:22681890
ubp3ubiquitin C-terminal hydrolase Ubp3 Negative GeneticPMID:22681890
rad9checkpoint clamp complex protein Rad9 Negative GeneticPMID:22681890
tor1phosphatidylinositol kinase Tor1 Negative GeneticPMID:22681890
SPBC1347.08cribonuclease H2 complex subunit (predicted) Positive GeneticPMID:22681890
sap14U2 snRNP-associated protein SF3B14 Sap14 Negative GeneticPMID:22681890
swd1Set1C complex subunit Swd1 Negative GeneticPMID:22681890
myo52myosin type V Synthetic LethalityPMID:21652630
sre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
rem1meiosis-specific cyclin Rem1 Negative GeneticPMID:22681890
SPBC56F2.08cRNA-binding protein (predicted) Negative GeneticPMID:22681890
SPAC821.05translation initiation factor eIF3h (p40) Positive GeneticPMID:22681890
ste20Rictor homolog, Ste20 Negative GeneticPMID:22681890
snf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Positive GeneticPMID:22681890
pck1protein kinase C (PKC)-like Pck1 Negative GeneticPMID:22681890
pmk1MAP kinase Pmk1 Negative GeneticPMID:22681890
bob1prefoldin subunit 5 (predicted) Positive GeneticPMID:22681890
rho3Rho family GTPase Rho3 Synthetic Growth DefectPMID:21652630
sck2serine/threonine protein kinase Sck2 Positive GeneticPMID:24463365
Phenotypic Enhancement
ies2Ino80 complex subunit Ies2 Positive GeneticPMID:22681890
cph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
clr1cryptic loci regulator Clr1 Negative GeneticPMID:22681890
erg5C-22 sterol desaturase Erg5 Positive GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
arp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
rpl160160S ribosomal protein L13/L16 (predicted) Positive GeneticPMID:22681890
ypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
sts5RNB-like protein Negative GeneticPMID:22681890
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Positive GeneticPMID:22681890
rga2Rho-type GTPase activating protein Rga2 Negative GeneticPMID:22681890
SPAC23A1.07ubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:22681890
usp102U1 snRNP-associated protein Usp102 Negative GeneticPMID:22681890
gma12alpha-1,2-galactosyltransferase Gma12 Negative GeneticPMID:22681890
php5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
nhp6High-mobility group non-histone chromatin protein (predicted) Positive GeneticPMID:22681890
kin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
pob3FACT complex subunit Pob3 Positive GeneticPMID:22681890
hrp1ATP-dependent DNA helicase Hrp1 Positive GeneticPMID:22681890
for3formin For3 Synthetic Growth DefectPMID:21652630
Phenotypic Enhancement
mkh1MEK kinase (MEKK) Mkh1 Negative GeneticPMID:22681890
dot2ESCRT II complex subunit Dot2 Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
Affinity Capture-Western
External References
Database Identifier Description
NBRP SPCC1739.10 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1739.10 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1739.10 BioGRID Interaction Datasets
Expression Viewer SPCC1739.10 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1739.10 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1739.10 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1739.10 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1739.10 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1739.10 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC1739.10 Cell Cycle Data
GEO SPCC1739.10 GEO profiles
PInt SPCC1739.10 Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2539381conserved fungal protein
EntrezGene2539381conserved fungal protein
SPD / RIKEN15/15A08Orfeome Localization Data
UniProtKB/SwissProtO74472Meiotically up-regulated gene 33 protein
ModBaseO74472Database of comparative protein structure models
STRINGO74472Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588418conserved fungal protein
RefSeq mRNANM_001023409972h- conserved fungal protein (mug33), mRNA
European Nucleotide ArchiveCAA20784ENA Protein Mapping
European Nucleotide ArchiveCAA20784.1ENA Protein Mapping
UniParcUPI000006C37BUniProt Archive

Literature for mug33

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014