mug33 (SPCC1739.10)


Gene Standard Namemug33 Characterisation Statuspublished
Systematic IDSPCC1739.10 Feature Typeprotein coding
Synonyms Name DescriptionMeiotically Upregulated Gene
ProductTea1-interacting protein involved in exocytosis Product Size336aa, 37.07 kDa
Genomic Location Chromosome III, 2047508-2049286 (1779nt); CDS:2047936-2048946 (1011nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
meiosis I86
Annotation ExtensionEvidenceWith/FromReference
positive regulation of exocytosis2
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell division site305
Annotation ExtensionEvidenceWith/FromReference
cell tip194
Annotation ExtensionEvidenceWith/FromReference
cytoplasm4207
Annotation ExtensionEvidenceWith/FromReference
endocytic vesicle8
Annotation ExtensionEvidenceWith/FromReference
exocytic vesicle1
Annotation ExtensionEvidenceWith/FromReference
growing cell tip24
Annotation ExtensionEvidenceWith/FromReference
plasma membrane249
Annotation ExtensionEvidenceWith/FromReference
plasma membrane of cell tip11
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased septation indexmug33ΔNull20
normal cell population growth at high temperaturemug33ΔNull47
normal growth on glucose carbon sourcemug33ΔNull203
sensitive to bortezomibmug33ΔNull256
sensitive to camptothecinmug33ΔNull224
sensitive to UV during vegetative growthmug33ΔNull141
viable vegetative cell populationmug33ΔNull3795

Cell Phenotype

Term NameAlleleExpressionCount
curved cell during recovery from stationary phasemug33ΔNull2
normal cell morphology during recovery from stationary phasemug33ΔNull1
normal endocytosismug33ΔNull2
normal protein localization to cell division site28
affecting sec10mug33ΔNull
affecting sec6mug33ΔNull
affecting sec8mug33ΔNull
normal protein localization to cell tip21
affecting sec10mug33ΔNull
affecting sec6mug33ΔNull
affecting sec8mug33ΔNull
normal protein secretionmug33ΔNull4
protein mislocalized to eisosome1
affecting mug33ΔC-terminus (181-336)Not specified
T-shaped cell during recovery from stationary phasemug33ΔNull2
viable vegetative cell with normal cell morphologymug33ΔNull3093
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in end4 Huntingtin-interacting protein homolog PMID:21652630
FYPO affected by mutation in for3 formin For3 PMID:21652630
FYPO affected by mutation in mug33 Tea1-interacting protein involved in exocytosis PMID:21652630
FYPO affected by mutation in myo52 myosin type V PMID:21652630
FYPO affected by mutation in rho3 Rho family GTPase Rho3 PMID:21652630
FYPO affected by mutation in sec8 exocyst complex subunit Sec8 PMID:21652630
FYPO affected by mutation in tea1 cell end marker Tea1 PMID:21652630
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2047508..2049286
mRNA2047508..2049286
5' UTR2047508..2047935PMID:21511999
CDS2047936..2048946
3' UTR2048947..2049286PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF06687 Pfam IPR009571 Actin cortical patch SUR7/pH-response regulator PalI 6 165 3
TMhelix TMHMM 89 111 959
TMhelix TMHMM 150 172 959
TMhelix TMHMM 118 140 959
TMhelix TMHMM 9 31 959
SignalP-TM signalp 1 23 16

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0000418signal_peptide279
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 110.34 Da
Charge 17.00
Isoelectric point 10.15
Molecular weight 37.07 kDa
Number of residues 336
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
level fluctuates during mitotic cell cycleS308
S233
present during mitotic M phaseS255
present during mitotic M phaseS233
present during mitotic M phaseS316
present during mitotic M phaseS252
present during mitotic M phaseS257
Annotation ExtensionEvidenceResidueReference
experimental evidence S233 PMID:24763107
present during mitotic M phase experimental evidence S233 PMID:21712547
present during mitotic M phase experimental evidence S252 PMID:21712547
present during mitotic M phase experimental evidence S255 PMID:21712547
present during mitotic M phase experimental evidence S257 PMID:21712547
level fluctuates during mitotic cell cycle experimental evidence S308 PMID:24763107
present during mitotic M phase experimental evidence S316 PMID:21712547
phosphorylated residue 1926
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0051321experimental evidencePMID:16303567

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
25366during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
23073during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
23405during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
25339during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
4481.14during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
25998during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
653.94during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
9.5during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
4.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only620
conserved in fungi4604
conserved in eukaryotes4516
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
affinity captured bytea1cell end marker Tea1 Affinity Capture-WesternPMID:21652630
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction witharp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
positive genetic interaction withbob1prefoldin subunit 5 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withclr1SHREC complex subunit Clr1 Negative GeneticPMID:22681890
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
negative genetic interaction withcwf19complexed with Cdc5 protein Cwf19 Negative GeneticPMID:22681890
negative genetic interaction withdot2ESCRT II complex subunit Dot2 Negative GeneticPMID:22681890
positive genetic interaction witherg5C-22 sterol desaturase Erg5 Positive GeneticPMID:22681890
phenotype enhanced byfor3formin For3 Phenotypic EnhancementPMID:21652630
synthetic growth defect withfor3formin For3 Synthetic Growth DefectPMID:21652630
negative genetic interaction withgma12alpha-1,2-galactosyltransferase Gma12 Negative GeneticPMID:22681890
positive genetic interaction withhrp1ATP-dependent DNA helicase Hrp1 Positive GeneticPMID:22681890
positive genetic interaction withies2Ino80 complex subunit Ies2 Positive GeneticPMID:22681890
negative genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
negative genetic interaction withmkh1MEK kinase (MEKK) Mkh1 Negative GeneticPMID:22681890
synthetic lethal withmyo52myosin type V Synthetic LethalityPMID:21652630
positive genetic interaction withnhp6High-mobility group non-histone chromatin protein (predicted) Positive GeneticPMID:22681890
negative genetic interaction withpck1protein kinase C (PKC)-like Pck1 Negative GeneticPMID:22681890
negative genetic interaction withphp5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
negative genetic interaction withpmk1MAP kinase Pmk1 Negative GeneticPMID:22681890
positive genetic interaction withpob3FACT complex subunit Pob3 Positive GeneticPMID:22681890
negative genetic interaction withrad9checkpoint clamp complex protein Rad9 Negative GeneticPMID:22681890
negative genetic interaction withrem1meiosis-specific cyclin Rem1 Negative GeneticPMID:22681890
negative genetic interaction withrga2Rho-type GTPase activating protein Rga2 Negative GeneticPMID:22681890
synthetic growth defect withrho3Rho family GTPase Rho3 Synthetic Growth DefectPMID:21652630
positive genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withsap14U2 snRNP-associated protein SF3B14 Sap14 Negative GeneticPMID:22681890
positive genetic interaction withsck2serine/threonine protein kinase Sck2 Positive GeneticPMID:24463365
enhances phenotype ofsck2serine/threonine protein kinase Sck2 Phenotypic EnhancementPMID:24463365
negative genetic interaction withslm1cytoskeletal signaling protein Slm1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsnf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
positive genetic interaction withSPAC23A1.07ubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPBC1347.08cribonuclease H2 complex subunit (predicted) Positive GeneticPMID:22681890
negative genetic interaction withSPBC56F2.08cRNA-binding protein (predicted) Negative GeneticPMID:22681890
positive genetic interaction withsrb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Positive GeneticPMID:22681890
negative genetic interaction withsre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
negative genetic interaction withsrk1MAPK-activated protein kinase Srk1 Negative GeneticPMID:22681890
negative genetic interaction withste20Rictor homolog, Ste20 Negative GeneticPMID:22681890
negative genetic interaction withsts5RNB-like protein Negative GeneticPMID:22681890
negative genetic interaction withswd1Set1C complex subunit Swd1 Negative GeneticPMID:22681890
negative genetic interaction withtaz1human TRF ortholog Taz1 Negative GeneticPMID:22681890
positive genetic interaction withtif38translation initiation factor eIF3h (p40) Positive GeneticPMID:22681890
negative genetic interaction withtor1phosphatidylinositol kinase Tor1 Negative GeneticPMID:22681890
negative genetic interaction withubp3ubiquitin C-terminal hydrolase Ubp3 Negative GeneticPMID:22681890
negative genetic interaction withusp102U1 snRNP-associated protein Usp102 Negative GeneticPMID:22681890
negative genetic interaction withxap5xap-5-like protein Negative GeneticPMID:24957674
negative genetic interaction withypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
positive genetic interaction withypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPCC1739.10 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1739.10 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1739.10 BioGRID Interaction Datasets
Expression Viewer SPCC1739.10 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1739.10 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1739.10 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1739.10 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1739.10 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1739.10 Transcriptome Viewer (Bähler Lab)
GEO SPCC1739.10 GEO profiles
PInt SPCC1739.10 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC1739.10 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC1739.10 Fission yeast phenotypic data & analysis
Cyclebase SPCC1739.10.1 Cell Cycle Data
SPD / RIKEN15/15A08Orfeome Localization Data
UniProtKB/SwissProtO74472Meiotically up-regulated gene 33 protein
ModBaseO74472Database of comparative protein structure models
STRINGO74472Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588418conserved fungal protein
RefSeq mRNANM_001023409972h- conserved fungal protein (mug33), mRNA
European Nucleotide ArchiveCAA20784.1ENA Protein Mapping
UniParcUPI000006C37BUniProt Archive

Literature for mug33

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015