ssa2 (SPCC1739.13)


Gene Standard Namessa2 Characterisation Statuspublished
Systematic IDSPCC1739.13 Feature Typeprotein coding
Synonymscsp6 Name Description
Productheat shock protein Ssa2 Product Size647aa, 70.23 kDa
Genomic Location Chromosome III, 2057084-2059331 (2248nt); CDS:2057243-2059186 (1944nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding560
Annotation ExtensionEvidenceWith/FromReference
ATPase activity239
Annotation ExtensionEvidenceWith/FromReference
unfolded protein binding42
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

protein folding


Term NameCount
chaperone-mediated protein folding10
Annotation ExtensionEvidenceWith/FromReference
negative regulation of cellular response to heat2
Annotation ExtensionEvidenceWith/FromReference
protein folding100
Annotation ExtensionEvidenceWith/FromReference
protein refolding8
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
nucleus2691
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viability depends on conditions

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationssa2Δ1452
resistance to heat shock during vegetative growthssa2Δ7
slow vegetative cell population growthssa2Δ361

Cell Phenotype

Term NameGenotypesCount
abolished protein localization to non-growing cell tip6
affects localization of cdc42ssa2Δ
altered mRNA level during vegetative growthssa2Δ117
decreased activation of bipolar cell growthssa2Δ7
decreased protein localization to cell cortex30
affects localization of rga4ssa2Δ
decreased protein localization to growing cell tip6
affects localization of cdc42ssa2Δ
increased protein level during cellular response to heat3
affecting hsp90ssa2Δ
affecting psi1ssa2Δ
intermittent monopolar cell growthssa2Δ2
inviable sporessa2Δ476
normal protein localization to cell tip24
affecting pom1ssa2Δ
affecting tea4ssa2Δ
affecting tea1ssa2Δ
unstable protein localization to growing cell tip2
affects localization of cdc42ssa2Δ

FYPO Multi-allele Phenotypes

Cell Phenotype

Term NameGenotypes
decreased misfolded protein degradation
affecting sdj1ssa2Δ, L169P (L169P)
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in lkh1 dual specificity protein kinase Lkh1
FYPO affected by mutation in sdj1 glyoxylase III sdj1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2057084..2059331
mRNA2057084..2059331
5' UTR2057084..2057242AB012387
CDS2057243..2059186
3' UTR2059187..2059331AB012387
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00012 Pfam IPR013126 Heat shock protein 70 family 5 610 8
PS00329 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 195 208 6
PS00297 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 7 14 5
PS01036 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 332 346 7
PTHR19375 HMMPANTHER 2 647 8
PTHR19375:SF140 HMMPANTHER 2 647 2
2.60.34.10 Gene3D IPR029047 Heat shock protein 70kD, peptide-binding domain 390 516 7
3.30.420.40 Gene3D Hexokinase 4 52 24
3.30.420.40 Gene3D Hexokinase 121 197 24
3.30.30.30 Gene3D Chaperone DnaK 53 113 6
1.20.1270.10 Gene3D IPR029048 Heat shock protein 70kD, C-terminal domain 517 621 6
3.90.640.10 Gene3D Actin-related protein 2 229 309 16
3.30.420.40 Gene3D Hexokinase 314 356 24
3.30.420.40 Gene3D Hexokinase 198 228 24
SSF53067 SuperFamily 188 380 25
SSF100934 SuperFamily IPR029048 Heat shock protein 70kD, C-terminal domain 535 617 7
SSF53067 SuperFamily 1 185 25
SSF100920 SuperFamily IPR029047 Heat shock protein 70kD, peptide-binding domain 384 541 7
PR00301 PRINTS IPR013126 Heat shock protein 70 family 140 160 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 388 407 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 53 61 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 3 16 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 469 485 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 361 381 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 31 43 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 201 211 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 329 345 8

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.55 Da
Charge -15.00
Codon Adaptation Index 0.81
Isoelectric point 4.87
Molecular weight 70.23 kDa
Number of residues 647
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1677
present during mitotic M phaseS423
present during mitotic M phaseS563
present during cellular response to thiabendazoleT417
present during cellular response to thiabendazoleS151
present during cellular response to thiabendazoleS546
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S423 PMID:21712547
present during mitotic M phase experimental evidence S563 PMID:21712547
present during cellular response to thiabendazole IDA T417 PMID:18257517
present during cellular response to thiabendazole IDA S151 PMID:18257517
present during cellular response to thiabendazole IDA S546 PMID:18257517
O-phospho-L-threonine 701
present during mitotic M phaseT567
present during mitotic M phaseT583
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T567 PMID:21712547
present during mitotic M phase experimental evidence T583 PMID:21712547
phosphorylated residue 1932
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
ubiquitinylated lysineK54, K95, K106, K136, K157, K323, K456, K498, K505, K510, K522, K595 512
Annotation ExtensionEvidenceResidueReference
IDA PMID:26412298
mass spectrometry evidence K157 PMID:26412298
mass spectrometry evidence K456 PMID:26412298
mass spectrometry evidence K95 PMID:26412298
mass spectrometry evidence K54 PMID:26412298
mass spectrometry evidence K522 PMID:26412298
mass spectrometry evidence K498 PMID:26412298
mass spectrometry evidence K136 PMID:26412298
mass spectrometry evidence K595 PMID:26412298
mass spectrometry evidence K106 PMID:26412298
mass spectrometry evidence K510 PMID:26412298
mass spectrometry evidence K323 PMID:26412298
mass spectrometry evidence K505 PMID:26412298
sumoylated lysine 173
Annotation ExtensionEvidenceResidueReference
IDA PMID:26404184
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for ssa2 (SPCC1739.13)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1001281during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
914262during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
934379during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1005018during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
902084during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
364293.29during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
68852.31during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
240during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
19during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Miscellaneous Functional Group
DescriptionQualifierReferenceCount
chaperone12
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4606
conserved in eukaryotes4516
conserved in bacteria1002
conserved in metazoa3424
conserved in vertebrates3399
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPCC1739.13 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bymas5DNAJ domain protein Mas5 (predicted) Affinity Capture-LuminescencePMID:24146635
affinity captured byint6eIF3e subunit Int6 Affinity Capture-MSPMID:16278451
affinity captured byopi10hikeshi protein, Opi10 Affinity Capture-MSPMID:24768994
affinity captured bycwf10U5 snRNP GTPase subunit Cwf10 Affinity Capture-MSPMID:24442611
affinity captured byrec10meiotic recombination protein Rec10 Affinity Capture-MSPMID:19756689
affinity captured bypaa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:24634168
affinity captured bysog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
affinity captured byrad52DNA recombination protein, Rad51 mediator Rad52 (previously Rad22) Affinity Capture-MSPMID:23779158
affinity captured bynak1PAK-related kinase Nak1 Affinity Capture-MSPMID:23462181
affinity captured bycdk11serine/threonine protein kinase cdk11 Affinity Capture-MSPMID:23122962
affinity captured bydsc1Golgi Dsc E3 ligase complex subunit Dsc1 Affinity Capture-MSPMID:21504829
affinity captured byrad2414-3-3 protein Rad24 Affinity Capture-WesternPMID:20231270
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
affinity captured byddb1damaged DNA binding protein Ddb1 Affinity Capture-MSPMID:18794354
affinity captured bylea1U2 snRNP-associated protein Lea1 (predicted) Affinity Capture-MSPMID:24442611
External References
Database Identifier Description
NBRP SPCC1739.13 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1739.13 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1739.13 BioGRID Interaction Datasets
Expression Viewer SPCC1739.13 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1739.13 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1739.13 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1739.13 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1739.13 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1739.13 Transcriptome Viewer (Bähler Lab)
GEO SPCC1739.13 GEO profiles
PInt SPCC1739.13 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC1739.13 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC1739.13 Fission yeast phenotypic data & analysis
Cyclebase SPCC1739.13.1 Cell Cycle Data
SPD / RIKEN26/26H10Orfeome Localization Data
UniProtKB/SwissProtO59855Probable heat shock protein ssa2
ModBaseO59855Database of comparative protein structure models
STRINGO59855Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588421heat shock protein Ssa2 (predicted)
RefSeq mRNANM_001023412972h- heat shock protein Ssa2 (predicted) (ssa2), mRNA
European Nucleotide ArchiveAB012387ENA EMBL mapping
European Nucleotide ArchiveCAA20787.1ENA Protein Mapping
UniParcUPI0000161F36UniProt Archive

Literature for ssa2

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016