ssa2 (SPCC1739.13)


Gene Standard Namessa2 Characterisation Statuspublished
Systematic IDSPCC1739.13 Feature Typeprotein coding
Synonyms Name Description
Productheat shock protein Ssa2 Product Size647aa, 70.23 kDa
Genomic Location Chromosome III, 2057084-2059331 (2248nt); CDS:2057243-2059186 (1944nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037558
GO:0016887ATPase activityISOSGD:S000000004GO_REF:0000024232
GO:0051082unfolded protein bindingISOSGD:S000000004GO_REF:000002442
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0061077chaperone-mediated protein foldingNASGO_REF:000000110
GO:1900035negative regulation of cellular response to heatIMPPMID:241466352
GO:0006457protein foldingISOSGD:S000000004GO_REF:0000024106
GO:0042026protein refoldingISOSGD:S000000004GO_REF:00000246
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722315
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viability depends on conditions

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopyssa2ΔNullPECO:0000005, PECO:0000137PMID:236978061338
FYPO:0001090resistance to heat shock during vegetative growthCell growth assayssa2ΔNullPMID:241466357
FYPO:0001234slow vegetative cell population growthCell growth assayssa2ΔNullPECO:0000005PMID:2414663578

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002859abolished protein localization to non-growing cell tip6
affecting cdc42Microscopyssa2ΔNullPECO:0000005PMID:24146635
FYPO:0002934altered mRNA level during vegetative growthTranscript expression level evidencessa2ΔNullPMID:2414663523
FYPO:0003150decreased activation of bipolar cell growth5
expressivity FYPO_EXT:0000001Cell growth assayssa2ΔNullPMID:24146635
FYPO:0000929decreased protein localization to cell cortex20
affecting rga4Microscopyssa2ΔNullPECO:0000005PMID:24146635
FYPO:0002871decreased protein localization to growing cell tip5
affecting cdc42Microscopyssa2ΔNullPECO:0000005PMID:24146635
FYPO:0003152increased protein level during cellular response to heat2
affecting hsp90Microscopyssa2ΔNullPMID:24146635
affecting psi1Microscopyssa2ΔNullPMID:24146635
FYPO:0003155intermittent monopolar cell growth2
penetrance FYPO_EXT:0000001Microscopyssa2ΔNullPECO:0000005PMID:24146635
FYPO:0002151inviable spore465
penetrance FYPO_EXT:0000001Microscopyssa2ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0001587normal protein localization to cell tip17
affecting tea1Microscopyssa2ΔNullPECO:0000005PMID:24146635
affecting pom1Microscopyssa2ΔNullPECO:0000005PMID:24146635
affecting tea4Microscopyssa2ΔNullPECO:0000005PMID:24146635
FYPO:0003157unstable protein localization to growing cell tip2
affecting cdc42Microscopyssa2ΔNullPECO:0000005PMID:24146635
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in lkh1 dual specificity protein kinase Lkh1 PMID:23956636
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
120570842059331
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00012 Pfam IPR013126 Heat shock protein 70 family 5 610 8
PS00329 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 195 208 6
PS01036 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 332 346 7
PS00297 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 7 14 5
PTHR19375 HMMPANTHER 1 645 8
3.30.30.30 Gene3D 53 113 6
2.60.34.10 Gene3D 390 516 7
3.30.420.40 Gene3D 198 228 24
3.30.420.40 Gene3D 314 356 24
3.90.640.10 Gene3D 229 309 16
1.20.1270.10 Gene3D 517 621 6
3.30.420.40 Gene3D 121 197 24
3.30.420.40 Gene3D 4 52 24
SSF100920 SuperFamily Heat shock protein 70kD, peptide-binding domain 384 541 7
SSF53067 SuperFamily 1 185 25
SSF53067 SuperFamily 188 380 25
SSF100934 SuperFamily Heat shock protein 70kD, C-terminal domain 535 617 7
PR00301 PRINTS IPR013126 Heat shock protein 70 family 469 485 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 201 211 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 53 61 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 140 160 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 361 381 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 3 16 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 329 345 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 388 407 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 31 43 8

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.55 Da
Charge -15.00
Isoelectric point 4.87
Molecular weight 70.23 kDa
Number of residues 647
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineIDAS546PMID:182575171670
present during cellular response to thiabendazole
present during cellular response to thiabendazoleIDAS151PMID:18257517
present during cellular response to thiabendazoleIDAT417PMID:18257517
present during mitotic M phaseexperimental evidenceS563PMID:21712547
present during mitotic M phaseexperimental evidenceS423PMID:21712547
MOD:00047O-phospho-L-threonineexperimental evidenceT567PMID:21712547692
present during mitotic M phase
present during mitotic M phaseexperimental evidenceT583PMID:21712547
MOD:00696phosphorylated residueNASPMID:182575171922
NASPMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1001281during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
914262during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
934379during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1005018during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
902084during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
364293.29during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
68852.31during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
240during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
19during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Miscellaneous Functional Group
DescriptionQualifierReferenceCount
chaperone12
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4600
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3425
conserved in vertebrates3400
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Affinity Capture-MSPMID:21504829
rad52DNA recombination protein Rad52 (previously Rad22) Affinity Capture-MSPMID:23779158
ddb1damaged DNA binding protein Ddb1 Affinity Capture-MSPMID:18794354
rad2414-3-3 protein Rad24 Affinity Capture-WesternPMID:20231270
int6eIF3e subunit Int6 Affinity Capture-MSPMID:16278451
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
sog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
nak1PAK-related kinase Nak1 Affinity Capture-MSPMID:23462181
cwf10U5 snRNP GTPase subunit Cwf10 Affinity Capture-MSPMID:24442611
mas5DNAJ domain protein Mas5 (predicted) Affinity Capture-WesternPMID:24146635
cdk11serine/threonine protein kinase cdk11 Affinity Capture-MSPMID:23122962
paa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:24634168
lea1U2 snRNP-associated protein Lea1 (predicted) Affinity Capture-MSPMID:24442611
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
rec10meiotic recombination protein Rec10 Affinity Capture-MSPMID:19756689
opi10hikeshi protein, Opi10 Affinity Capture-MSPMID:24768994
External References
Database Identifier Description
NBRP SPCC1739.13 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1739.13 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1739.13 BioGRID Interaction Datasets
Expression Viewer SPCC1739.13 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1739.13 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1739.13 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1739.13 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1739.13 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1739.13 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC1739.13 Cell Cycle Data
GEO SPCC1739.13 GEO profiles
PInt SPCC1739.13 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC1739.13 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC1739.13 Fission yeast phenotypic data & analysis
SPD / RIKEN26/26H10Orfeome Localization Data
UniProtKB/SwissProtO59855Probable heat shock protein ssa2
ModBaseO59855Database of comparative protein structure models
STRINGO59855Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588421heat shock protein Ssa2 (predicted)
RefSeq mRNANM_001023412972h- heat shock protein Ssa2 (predicted) (ssa2), mRNA
European Nucleotide ArchiveAB012387ENA EMBL mapping
European Nucleotide ArchiveCAA20787.1ENA Protein Mapping
UniParcUPI0000161F36UniProt Archive

Literature for ssa2

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014