ssa2 (SPCC1739.13)


Gene Standard Namessa2 Characterisation Statuspublished
Systematic IDSPCC1739.13 Feature Typeprotein coding
Synonyms Name Description
Productheat shock protein Ssa2 Product Size647aa, 70.23 kDa
Genomic Location Chromosome III, 2057084-2059331 (2248nt); CDS:2057243-2059186 (1944nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037559
GO:0016887ATPase activityISOSGD:S000000004GO_REF:0000024231
GO:0051082unfolded protein bindingISOSGD:S000000004GO_REF:000002442
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0061077chaperone-mediated protein foldingNASGO_REF:000000111
GO:1900035negative regulation of cellular response to heatIMPPMID:241466352
GO:0006457protein foldingISOSGD:S000000004GO_REF:0000024111
GO:0042026protein refoldingISOSGD:S000000004GO_REF:00000246
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722316
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopyssa2ΔNullPECO:0000005, PECO:0000137PMID:236978061315
FYPO:0001090resistance to heat shock during vegetative growthCell growth assayssa2ΔNullPMID:241466356
FYPO:0001234slow vegetative cell population growthCell growth assayssa2ΔNullPECO:0000005PMID:2414663554

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002859abolished protein localization to non-growing cell tip6
affecting cdc42Microscopyssa2ΔNullPECO:0000005PMID:24146635
FYPO:0002934altered mRNA level during vegetative growthTranscript expression level evidencessa2ΔNullPMID:2414663520
FYPO:0003150decreased activation of bipolar cell growth5
expressivity FYPO_EXT:0000001Cell growth assayssa2ΔNullPMID:24146635
FYPO:0000929decreased protein localization to cell cortex16
affecting rga4Microscopyssa2ΔNullPECO:0000005PMID:24146635
FYPO:0002871decreased protein localization to growing cell tip5
affecting cdc42Microscopyssa2ΔNullPECO:0000005PMID:24146635
FYPO:0003152increased protein level during cellular response to heat2
affecting psi1Microscopyssa2ΔNullPMID:24146635
affecting hsp90Microscopyssa2ΔNullPMID:24146635
FYPO:0003155intermittent monopolar cell growth2
penetrance FYPO_EXT:0000001Microscopyssa2ΔNullPECO:0000005PMID:24146635
FYPO:0002151inviable spore457
penetrance FYPO_EXT:0000001Microscopyssa2ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0001587normal protein localization to cell tip14
affecting tea4Microscopyssa2ΔNullPECO:0000005PMID:24146635
affecting pom1Microscopyssa2ΔNullPECO:0000005PMID:24146635
affecting tea1Microscopyssa2ΔNullPECO:0000005PMID:24146635
FYPO:0003157unstable protein localization to growing cell tip2
affecting cdc42Microscopyssa2ΔNullPECO:0000005PMID:24146635
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in lkh1 dual specificity protein kinase Lkh1 PMID:23956636
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
120570842059331
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00012 Pfam IPR013126 Heat shock protein 70 family 5 610 8
PS01036 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 332 346 7
PS00297 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 7 14 5
PS00329 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 195 208 6
PTHR19375:SF142 HMMPANTHER 1 647 2
PTHR19375 HMMPANTHER 1 647 8
3.30.420.40 Gene3D 121 197 24
3.30.420.40 Gene3D 4 52 24
3.30.420.40 Gene3D 314 356 24
3.30.420.40 Gene3D 198 228 24
3.30.30.30 Gene3D 53 113 6
1.20.1270.10 Gene3D IPR029048 Heat shock protein 70kD, C-terminal domain 517 621 6
2.60.34.10 Gene3D IPR029047 Heat shock protein 70kD, peptide-binding domain 390 516 7
3.90.640.10 Gene3D 229 309 16
SSF100934 SuperFamily IPR029048 Heat shock protein 70kD, C-terminal domain 535 617 7
SSF100920 SuperFamily IPR029047 Heat shock protein 70kD, peptide-binding domain 384 541 7
SSF53067 SuperFamily 188 380 25
SSF53067 SuperFamily 1 185 25
PR00301 PRINTS IPR013126 Heat shock protein 70 family 388 407 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 31 43 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 361 381 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 329 345 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 53 61 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 140 160 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 201 211 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 469 485 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 3 16 8

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.55 Da
Charge -15.00
Isoelectric point 4.87
Molecular weight 70.23 kDa
Number of residues 647
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineIDAT417PMID:182575171663
present during cellular response to thiabendazole
present during mitotic M phaseS563PMID:21712547
present during mitotic M phaseS423PMID:21712547
present during cellular response to thiabendazoleIDAS546PMID:18257517
present during cellular response to thiabendazoleIDAS151PMID:18257517
MOD:00047O-phospho-L-threonineT583PMID:21712547682
present during mitotic M phase
present during mitotic M phaseT567PMID:21712547
MOD:00696phosphorylated residueNASPMID:182575171915
NASPMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
364293.29during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
68852.31during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
902084during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
914262during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
1001281during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
1005018during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
934379during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
19during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
240during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
Miscellaneous Functional Group
DescriptionQualifierReferenceCount
chaperone12
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4569
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3393
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Affinity Capture-MSPMID:21504829
rad52DNA recombination protein Rad52 (previously Rad22) Affinity Capture-MSPMID:23779158
ddb1damaged DNA binding protein Ddb1 Affinity Capture-MSPMID:18794354
rad2414-3-3 protein Rad24 Affinity Capture-WesternPMID:20231270
int6eIF3e subunit Int6 Affinity Capture-MSPMID:16278451
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
sog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
nak1PAK-related kinase Nak1 Affinity Capture-MSPMID:23462181
cwf10U5 snRNP GTPase subunit Cwf10 Affinity Capture-MSPMID:24442611
mas5DNAJ domain protein Mas5 (predicted) Affinity Capture-WesternPMID:24146635
cdk11serine/threonine protein kinase cdk11 Affinity Capture-MSPMID:23122962
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
lea1U2 snRNP-associated protein Lea1 (predicted) Affinity Capture-MSPMID:24442611
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
rec10meiotic recombination protein Rec10 Affinity Capture-MSPMID:19756689
External References
Database Identifier Description
NBRP SPCC1739.13 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1739.13 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1739.13 BioGRID Interaction Datasets
Expression Viewer SPCC1739.13 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1739.13 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1739.13 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1739.13 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1739.13 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1739.13 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC1739.13 Cell Cycle Data
GEO SPCC1739.13 GEO profiles
PInt SPCC1739.13 Protein-Protein Interaction Predictor (Bähler Lab)
EntrezGene2539225heat shock protein Ssa2
WikiGene2539225heat shock protein Ssa2
SPD / RIKEN26/26H10Orfeome Localization Data
UniProtKB/SwissProtO59855Probable heat shock protein ssa2
ModBaseO59855Database of comparative protein structure models
STRINGO59855Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588421heat shock protein Ssa2
RefSeq mRNANM_001023412972h- heat shock protein Ssa2 (ssa2), mRNA
European Nucleotide ArchiveAB012387ENA EMBL mapping
European Nucleotide ArchiveBAA25322ENA Protein Mapping
European Nucleotide ArchiveCAA20787ENA Protein Mapping
European Nucleotide ArchiveCAA20787.1ENA Protein Mapping
UniParcUPI0000161F36UniProt Archive

Literature for ssa2

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014