sal3 (SPCC1840.03)


Gene Standard Namesal3 Characterisation Statuspublished
Systematic IDSPCC1840.03 Feature Typeprotein coding
Synonymspse1 Name Description
Productkaryopherin Sal3 Product Size1095aa, 121.87 kDa
Genomic Location Chromosome III, 2259995-2263761 (3767nt); CDS:2260282-2263569 (3288nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
GTP binding113
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cellular protein localization507
Annotation ExtensionEvidenceWith/FromReference
protein import into nucleus22
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4211
Annotation ExtensionEvidenceWith/FromReference
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
nuclear envelope129
Annotation ExtensionEvidenceWith/FromReference
nuclear membrane33
Annotation ExtensionEvidenceWith/FromReference
nuclear periphery45
Annotation ExtensionEvidenceWith/FromReference
nuclear pore51
Annotation ExtensionEvidenceWith/FromReference
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
protein complex1354
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased mating efficiencysal3Δ272
inviable vegetative cell populationsal3+ (wild type)1451
loss of viability at low temperaturesal3Δ5
resistance to tamoxifensal3Δ61
viable vegetative cell population3840
sal3Δ

Cell Phenotype

Term NameGenotypesCount
abnormal microtubule cytoskeleton morphology during mitotic interphasesal3Δ186
abnormal mitotic cell cyclesal3Δ877
abolished protein localization to nucleus during vegetative growth60
affects localization of clp1sal3Δ
elongated vegetative cell793
microtubule bundles present in increased numberssal3Δ23
normal protein import into nucleussal3Δ15
normal protein localization to actomyosin contractile ring15
affecting clp1sal3Δ
normal protein localization to mitotic spindle pole body during interphase2
affecting clp1sal3Δ
S-shaped cellsal3Δ27
viable elongated vegetative cellsal3Δ318
viable vegetative cell with abnormal cell shapesal3Δ700
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2259995..2263761
mRNA2259995..2263761
5' UTR2259995..2260281PMID:21511999
CDS2260282..2263569
3' UTR2263570..2263761PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13513 Pfam 385 439 4
PTHR10527:SF5 HMMPANTHER 11 1088 1
PTHR10527 HMMPANTHER 11 1088 4
1.25.10.10 Gene3D IPR011989 Armadillo-like helical 597 725 86
1.25.10.10 Gene3D IPR011989 Armadillo-like helical 3 549 86
1.25.10.10 Gene3D IPR011989 Armadillo-like helical 726 1093 86
SSF48371 SuperFamily IPR016024 Armadillo-type fold 13 641 130
SSF48371 SuperFamily IPR016024 Armadillo-type fold 828 942 130
SSF48371 SuperFamily IPR016024 Armadillo-type fold 678 768 130

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000328HEAT repeatTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000328
PBO:0000561importin familyPMID:12399381Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000561

Protein Properties

Ave. residue weight 111.30 Da
Charge -54.50
Codon Adaptation Index 0.52
Isoelectric point 4.36
Molecular weight 121.87 kDa
Number of residues 1095
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for sal3 (SPCC1840.03)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
13468during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
13955during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
14134during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
13603during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
10272.12during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
14075during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2210.26during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
8.6during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2506
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPCC1840.03 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byclp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
affinity captured bymlo3RNA binding protein Mlo3 Affinity Capture-MSPMID:21436456
affinity captured bypaa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
affinity captured byfar8SIP/FAR complex striatin subunit, Far8/Csc3 Affinity Capture-MSPMID:22119525
affinity capturesclp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-WesternPMID:23297348
affinity capturesphp4CCAAT-binding factor complex subunit Php4 Affinity Capture-WesternPMID:25330182
affinity capturesrdp1RNA-directed RNA polymerase Rdp1 Affinity Capture-WesternPMID:22268381
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPCC1840.03 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
enhances phenotype ofint6eIF3e subunit Int6 Phenotypic EnhancementPMID:19818717
synthetic growth defect withint6eIF3e subunit Int6 Synthetic Growth DefectPMID:19818717
synthetic lethal withcdc25M phase inducer tyrosine phosphatase Cdc25 Synthetic LethalityPMID:12399381
synthetic lethal withkap123karyopherin Kap123 Synthetic LethalityPMID:19818717
External References
Database Identifier Description
NBRP SPCC1840.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1840.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1840.03 BioGRID Interaction Datasets
Expression Viewer SPCC1840.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1840.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1840.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1840.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1840.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1840.03 Transcriptome Viewer (Bähler Lab)
GEO SPCC1840.03 GEO profiles
PInt SPCC1840.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC1840.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC1840.03 Fission yeast phenotypic data & analysis
Cyclebase SPCC1840.03.1 Cell Cycle Data
SPD / RIKEN30/30C10Orfeome Localization Data
UniProtKB/SwissProtO74476Importin subunit beta-3
ModBaseO74476Database of comparative protein structure models
STRINGO74476Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588502karyopherin Sal3
RefSeq mRNANM_001023492972h- karyopherin Sal3 (sal3), mRNA

Literature for sal3

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016