wee1 (SPCC18B5.03)


Gene Standard Namewee1 Characterisation Statuspublished
Systematic IDSPCC18B5.03 Feature Typeprotein coding
Synonyms Name Description
ProductM phase inhibitor protein kinase Wee1 Product Size877aa, 96.26 kDa
Genomic Location Chromosome III, 721788-725835 (4048nt); CDS:722234-724867 (2634nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037558
GO:0004693cyclin-dependent protein serine/threonine kinase activity8
has substrate cdc2IDAPMID:1372994
GO:0046872metal ion bindingIEAUniProtKB-KW:KW-0479GO_REF:0000037748
GO:0004715non-membrane spanning protein tyrosine kinase activityIEA EC:2.7.10.2GO_REF:00000032
GO:0005515protein bindingIPIcdc2PMID:1372994865
GO:0004674protein serine/threonine kinase activity112
has substrate wee1IDAPMID:8256510
GO:0004713protein tyrosine kinase activity9
has substrate wee1IDAPMID:8256510
has substrate wee1IDAPMID:1825699
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0044773mitotic DNA damage checkpointTASPMID:154917943
GO:0007093mitotic cell cycle checkpointIMPPMID:1604967988
GO:0010972negative regulation of G2/M transition of mitotic cell cycleIMPPMID:2262465150
IMPPMID:3032459
GO:0044387negative regulation of protein kinase activity by regulation of protein phosphorylation1
regulates cdc2IDAPMID:1372994
GO:0036289peptidyl-serine autophosphorylationIDAPMID:82565102
GO:0038083peptidyl-tyrosine autophosphorylationIDAPMID:82565105
GO:0008361regulation of cell sizeNASGO_REF:00000016
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0071341medial cortical node10
localization requires cdr2IDAPMID:19474792
GO:0044732mitotic spindle pole bodyIDAPMID:19474789214
IDAPMID:16823372
GO:0005654nucleoplasmTASPMID:9034337231
GO:0005634nucleusIDAPMID:194747892737
IDAPMID:7813446
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0003535decreased bipolar indexMicroscopywee1ΔNullPECO:0000004PMID:2050195427
Microscopywee1ΔNullPECO:0000005PMID:20501954
FYPO:0000046decreased cell population growthCell growth assaywee1ΔNullPECO:0000081PMID:24815688382
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assaywee1ΔNullPECO:0000201, PECO:0000102, PECO:0000004PMID:9487130119
FYPO:0003532increased monopolar indexMicroscopywee1ΔNullPECO:0000005PMID:2050195435
Microscopywee1ΔNullPECO:0000004PMID:20501954
FYPO:0000650increased septation indexMicroscopywee1-50Not specifiedPECO:0000005PMID:154917936
FYPO:0002061inviable vegetative cell populationCell growth assaywee1+ (wild type)OverexpressionPECO:0000126, PECO:0000102PMID:81634911337
FYPO:0000088sensitive to hydroxyurea513
expressivity FYPO_EXT:0000002Cell Growth Assaywee1ΔNullPECO:0000137, PECO:0000102PMID:23697806
FYPO:0000267sensitive to ionizing radiationCell growth assaywee1-50Not specifiedPECO:0000137, PECO:0000004PMID:154917944
FYPO:0002060viable vegetative cell populationMicroscopywee1ΔNullPMID:204732893751
Microscopywee1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001931abnormal cell cycle regulation during cellular response to gamma radiationMicroscopywee1-50Not specifiedPECO:0000004PMID:15491795
FYPO:0001248abnormal regulation of DNA replication initiationMicroscopywee1-50Not specifiedPECO:0000004PMID:87289011
FYPO:0001384abolished protein kinase activityWestern blot assayK596L (K596L)Not specifiedPMID:182569914
FYPO:0001024cell cycle arrest phenotypeMicroscopywee1-50Not specifiedPECO:0000006PMID:1464318136
FYPO:0001122elongated vegetative cellMicroscopywee1+ (wild type)OverexpressionPMID:12237855663
FYPO:0002020increased RNA level during nitrogen starvation13
affecting ste11Transcript expression level evidencewee1-50Not specifiedPMID:9135083
FYPO:0000825increased RNA level during vegetative growth122
affecting ste11Transcript expression level evidencewee1-50Not specifiedPMID:9135083
FYPO:0001490inviable elongated vegetative cellMicroscopywee1+ (wild type)OverexpressionPMID:8163491389
FYPO:0001489inviable vegetative cellMicroscopywee1-50OverexpressionPECO:0000005PMID:14643191195
FYPO:0000333mitotic G1/S transition delayFlow cytometry datawee1-50Not specifiedPMID:211189607
FYPO:0001929normal cell cycle regulation during cellular response to hydroxyureaMicroscopywee1-50Not specifiedPECO:0000004PMID:15491797
Microscopywee1-50Not specifiedPECO:0000005PMID:1549179
FYPO:0001221normal nucleus:cytoplasm ratioMicroscopywee1-50EndogenousPECO:0000004PMID:179984016
FYPO:0002559normal protein localization to actomyosin contractile ring14
affecting nod1Microscopywee1-50Not specifiedPMID:23349808
FYPO:0002558normal protein localization to medial cortex9
affecting nod1Microscopywee1-50Not specifiedPMID:23349808
FYPO:0000776normal protein phosphorylation23
affecting cdc2Gel electrophoresis evidencewee1-50Not specifiedPECO:0000004PMID:3058333
FYPO:0001124normal vegetative cell sizeMicroscopywee1-50Not specifiedPECO:0000005PMID:14643198
FYPO:0001492viable elongated vegetative cellMicroscopywee1-50Not specifiedPECO:0000005PMID:1464318197
FYPO:0000648viable small vegetative cellMicroscopywee1-50Not specifiedPECO:0000126, PECO:0000004PMID:146431832
Microscopywee-115Not specifiedPECO:0000004PMID:672898
Microscopywee1-50Not specifiedPECO:0000004PMID:672898
Microscopywee1-1Not specifiedPECO:0000004PMID:672898
Cell growth assaywee1-50Not specifiedPECO:0000004PMID:1756736
penetrance FYPO_EXT:0000001Microscopywee1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopywee1-50Not specifiedPECO:0000004PMID:1464319
Microscopywee1ΔNullPECO:0000137PMID:22624651
Microscopywee1-6Not specifiedPECO:0000004PMID:672898
Microscopywee1-8Not specifiedPECO:0000004PMID:672898
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in cds1 replication checkpoint kinase Cds1 PMID:19357077
FYPO affected by mutation in dnt1 nucleolar protein Dnt1 PMID:24006256
FYPO affected by mutation in rad3 ATR checkpoint kinase Rad3 PMID:17189249
FYPO affected by mutation in rpn12 19S proteasome regulatory subunit Rpn12 PMID:24006256
FYPO affected by mutation in skp1 SCF ubiquitin ligase complex subunit Skp1 PMID:24006256
FYPO affected by mutation in tel2 Tel2/Rad-5/Clk-2 family protein Tel2 PMID:17189249
GO substrate of cdr1 NIM1 family serine/threonine protein kinase Cdr1/Nim1 PMID:7681363
GO localized by cdr2 serine/threonine protein kinase Cdr2 PMID:19474789
GO substrate of cdr2 serine/threonine protein kinase Cdr2 PMID:9843572
GO substrate of cds1 replication checkpoint kinase Cds1 PMID:19357077
GO substrate of pyp1 tyrosine phosphatase Pyp1 PMID:8256510
GO substrate of wee1 M phase inhibitor protein kinase Wee1 PMID:1825699
GO substrate of wee1 M phase inhibitor protein kinase Wee1 PMID:8256510
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1721788725835
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 567 837 109
SM00220 SMART IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain 566 840 107
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 689 701 97
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 572 597 73
PS50011 Prosite Profiles IPR000719 Protein kinase domain 566 843 110
PTHR11042:SF21 HMMPANTHER 239 872 2
PTHR11042 HMMPANTHER 239 872 5
1.10.510.10 Gene3D 646 836 112
3.30.200.20 Gene3D 563 645 111
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 545 836 127

View domain organization at Pfam

Protein Properties

Ave. residue weight 109.76 Da
Charge 28.00
Isoelectric point 10.00
Molecular weight 96.26 kDa
Number of residues 877
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineIDAPMID:18256991670
added by wee1
IDAPMID:1372994
added by wee1IDAPMID:8256510
MOD:00047O-phospho-L-threonineIDAPMID:1372994690
MOD:00048O4'-phospho-L-tyrosineIDAPMID:825651084
removed by pyp1
IDAPMID:1372994
added by wee1IDAPMID:1825699
added by wee1IDAPMID:8256510
MOD:00696phosphorylated residueNASPMID:195477441922
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0034599expression microarray evidencePMID:12529438

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
NDduring GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.8during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
trichostatin A delays mitotic entry in cdc25-22 mutant PMID:197238881
trichostatin A partially suppresses wee1delta sensitivity to UV and hydroxyurea PMID:197238881
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
res2MBF transcription factor complex subunit Res2 Synthetic RescuePMID:11029045
ams2cell cycle regulated GATA-type transcription factor Ams2 Synthetic LethalityPMID:18077559
wis1MAP kinase kinase Wis1 Dosage RescuePMID:1756736
Phenotypic Suppression
pcp1pericentrin Pcp1 Synthetic Growth DefectPMID:21233285
Phenotypic EnhancementPMID:19942852
cig2G1/S-specific B-type cyclin Cig2 Phenotypic SuppressionPMID:8455610
Synthetic Rescue
Phenotypic SuppressionPMID:8972180
hob1BAR adaptor protein Hob1 Phenotypic SuppressionPMID:12569356
klp6kinesin-like protein Klp6 Phenotypic EnhancementPMID:11739790
liz1pantothenate transporter Liz1 Phenotypic EnhancementPMID:9950674
slp1sleepy homolog Slp1 Synthetic RescuePMID:11058086
Phenotypic SuppressionPMID:9001228
Synthetic Rescue
cdr2serine/threonine protein kinase Cdr2 Synthetic RescuePMID:9843572
Phenotypic Suppression
Synthetic RescuePMID:2245912
cdr1NIM1 family serine/threonine protein kinase Cdr1/Nim1 Synthetic RescuePMID:16816416
Synthetic RescuePMID:2245912
pyp1tyrosine phosphatase Pyp1 Phenotypic SuppressionPMID:1448087
Synthetic RescuePMID:1464319
zfs1CCCH tandem zinc finger protein, human Tristetraprolin homolog Zfs1, involved in mRNA catabolism Phenotypic EnhancementPMID:10462526
SPAC29A4.02ctranslation elongation factor EF-1 gamma subunit Phenotypic SuppressionPMID:14985109
tor1phosphatidylinositol kinase Tor1 Phenotypic SuppressionPMID:19546237
Synthetic Lethality
atf1transcription factor, Atf-CREB family Atf1 Phenotypic SuppressionPMID:9135083
mik1mitotic inhibitor kinase Mik1 Synthetic LethalityPMID:11809834
Synthetic Growth DefectPMID:11029045
Phenotypic EnhancementPMID:10947840
Synthetic LethalityPMID:1706223
Dosage Rescue
Synthetic LethalityPMID:9398669
bgs11,3-beta-glucan synthase catalytic subunit Bgs1 Phenotypic SuppressionPMID:10704373
Phenotypic EnhancementPMID:10503548
sds21serine/threonine protein phosphatase PP1 subfamily, Sds21 Dosage RescuePMID:1944266
clp1Cdc14-related protein phosphatase Clp1/Flp1 Phenotypic EnhancementPMID:11683392
Phenotypic Suppression
dis33'-5' exoribonuclease subunit Dis3 (predicted) Dosage RescuePMID:1944266
rad3ATR checkpoint kinase Rad3 Synthetic LethalityPMID:9398669
mor2morphogenesis protein Mor2 Synthetic LethalityPMID:12234926
rum1CDK inhibitor Rum1 Phenotypic EnhancementPMID:9013342
Dosage RescuePMID:9571240
ssp1Ca2+/calmodulin-dependent (CaMMK)-like protein kinase Ssp1 Phenotypic SuppressionPMID:10233158
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic EnhancementPMID:3116001
Synthetic Growth DefectPMID:11029045
Phenotypic EnhancementPMID:9013342
Synthetic LethalityPMID:9188094
Synthetic LethalityPMID:2474475
sty1MAP kinase Sty1 Synthetic RescuePMID:7501024
orc1origin recognition complex subunit Orc1 Phenotypic EnhancementPMID:12186947
cdc16two-component GAP Cdc16 Phenotypic SuppressionPMID:20805322
rad1checkpoint clamp complex protein Rad1 Synthetic LethalityPMID:8590806
swr1SNF2 family helicase Swr1 Dosage RescuePMID:9571240
spt8SAGA complex subunit Spt8 Phenotypic EnhancementPMID:10392445
slm9hira protein Slm9 Synthetic Growth DefectPMID:9843572
Phenotypic SuppressionPMID:10835386
crb2DNA repair protein Rad9 homolog, Rhp9 Synthetic Growth DefectPMID:20679488
fin1serine/threonine protein kinase, NIMA related Fin1 Phenotypic SuppressionPMID:12065422
pal1membrane associated protein Pal1 Synthetic LethalityPMID:15975911
mcs4response regulator Mcs4 Synthetic RescuePMID:9383051
hus5SUMO conjugating enzyme Hus5 Synthetic Growth DefectPMID:7768995
med8mediator complex subunit Med8 Phenotypic EnhancementPMID:10392445
mcl1DNA polymerase alpha accessory factor Mcl1 Synthetic Growth DefectPMID:12455694
skb1type II protein arginine N-methyltransferase Skb1 Dosage LethalityPMID:9843966
Phenotypic SuppressionPMID:23615447
ini1RING finger-like protein Ini1 Phenotypic SuppressionPMID:14762117
rad31SUMO E1-like activator enzyme Rad31 Phenotypic EnhancementPMID:9092625
srw1CDK inhibitor Srw1 Synthetic LethalityPMID:9571240
Synthetic LethalityPMID:9398669
Dosage Rescue
klp5kinesin-like protein Klp5 Phenotypic EnhancementPMID:11739790
chk1Chk1 protein kinase Phenotypic EnhancementPMID:10637286
Synthetic LethalityPMID:8497322
Synthetic LethalityPMID:11270571
Synthetic RescuePMID:16816416
win1MAP kinase kinase kinase Win1 Phenotypic EnhancementPMID:3442828
Phenotypic Suppression
pof3F-box protein Pof3 Phenotypic SuppressionPMID:11809834
pab1protein phosphatase regulatory subunit Pab1 Phenotypic SuppressionPMID:20876564
dis2serine/threonine protein phosphatase PP1 subfamily, Dis2 Dosage RescuePMID:1944266
cdc14SIN component Cdc14 Synthetic RescuePMID:8334307
Dosage RescuePMID:24006256
fkh2forkhead transcription factor Fkh2 Phenotypic SuppressionPMID:22451489
mis3rRNA processing protein Mis3 Phenotypic EnhancementPMID:10947840
dbr1RNA lariat debranching enzyme Dbr1 Synthetic Growth DefectPMID:16049013
pyp2tyrosine phosphatase Pyp2 Phenotypic SuppressionPMID:1448087
pin1peptidyl-prolyl cis-trans isomerase Pin1 Synthetic Growth DefectPMID:11707530
reb1RNA polymerase I transcription termination factor Reb1 Phenotypic EnhancementPMID:21118960
Synthetic Lethality
prp12U2 snRNP-associated protein Sap130 Synthetic Growth DefectPMID:16049013
cdc25M phase inducer tyrosine phosphatase Cdc25 Phenotypic SuppressionPMID:9473044
Synthetic RescuePMID:7501024
Synthetic RescuePMID:11085271
Synthetic RescuePMID:2245912
Synthetic Growth DefectPMID:23333317
Phenotypic EnhancementPMID:9013342
Phenotypic Suppression
Phenotypic EnhancementPMID:10233158
Synthetic RescuePMID:9843572
Phenotypic SuppressionPMID:3442828
Phenotypic EnhancementPMID:11531413
Synthetic RescuePMID:7217015
cdc28ATP-dependent RNA helicase Cdc28 Synthetic Growth DefectPMID:16049013
yox1MBF complex corepressor Yox1 Phenotypic SuppressionPMID:21132016
ran1serine/threonine protein kinase Ran1 Phenotypic SuppressionPMID:10521402
SPAC144.17c6-phosphofructo-2-kinase (predicted) Phenotypic SuppressionPMID:17900713
cdc13G2/M B-type cyclin Cdc13 Dosage LethalityPMID:9398669
sep1forkhead transcription factor Sep1 Phenotypic EnhancementPMID:9473044
Phenotypic EnhancementPMID:10392445
pub1HECT-type ubiquitin-protein ligase E3 Pub1 Synthetic LethalityPMID:8635463
cdc3profilin Phenotypic SuppressionPMID:20805322
ppa2serine/threonine protein phosphatase Ppa2 Synthetic LethalityPMID:8389306
shk1PAK-related kinase Shk1 Dosage LethalityPMID:9843966
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
skb1type II protein arginine N-methyltransferase Skb1 Affinity Capture-WesternPMID:23615447
Two-hybrid
hsp90Hsp90 chaperone Affinity Capture-WesternPMID:11298745
chk1Chk1 protein kinase Biochemical ActivityPMID:9034337
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Biochemical ActivityPMID:7982971
Biochemical ActivityPMID:1850698
Biochemical ActivityPMID:1372994
cds1replication checkpoint kinase Cds1 Biochemical ActivityPMID:21099360
Affinity Capture-WesternPMID:9572736
Biochemical ActivityPMID:11606526
cdr1NIM1 family serine/threonine protein kinase Cdr1/Nim1 Biochemical ActivityPMID:8515817
Two-hybridPMID:9928955
Biochemical ActivityPMID:8515818
Two-hybridPMID:23615447
pyp1tyrosine phosphatase Pyp1 Biochemical ActivityPMID:8256510
cdr2serine/threonine protein kinase Cdr2 Biochemical ActivityPMID:9843572
pyp2tyrosine phosphatase Pyp2 Biochemical ActivityPMID:8256510
wos2p23 homolog, predicted co-chaperone Wos2 Affinity Capture-WesternPMID:11298745
External References
Database Identifier Description
NBRP SPCC18B5.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC18B5.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC18B5.03 BioGRID Interaction Datasets
Expression Viewer SPCC18B5.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC18B5.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC18B5.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC18B5.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC18B5.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC18B5.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC18B5.03 Cell Cycle Data
GEO SPCC18B5.03 GEO profiles
PInt SPCC18B5.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC18B5.03 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN47/47A06Orfeome Localization Data
UniProtKB/SwissProtP07527Mitosis inhibitor protein kinase wee1
ModBaseP07527Database of comparative protein structure models
STRINGP07527Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587933M phase inhibitor protein kinase Wee1
RefSeq mRNANM_001022924972h- M phase inhibitor protein kinase Wee1 (wee1), mRNA
European Nucleotide ArchiveAAA35354.1ENA Protein Mapping
European Nucleotide ArchiveBAA87204.1ENA Protein Mapping
European Nucleotide ArchiveCAB52150.1ENA Protein Mapping
UniParcUPI0000138EF6UniProt Archive

Literature for wee1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014