wee1 (SPCC18B5.03)


Gene Standard Namewee1 Characterisation Statuspublished
Systematic IDSPCC18B5.03 Feature Typeprotein coding
Synonyms Name Description
ProductM phase inhibitor protein kinase Wee1 Product Size877aa, 96.26 kDa
Genomic Location Chromosome III, 721788-725835 (4048nt); CDS:722234-724867 (2634nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding551
Annotation ExtensionEvidenceWith/FromReference
metal ion binding742
Annotation ExtensionEvidenceWith/FromReference
protein binding900
Annotation ExtensionEvidenceWith/FromReference
protein serine/threonine kinase activity105
Annotation ExtensionEvidenceWith/FromReference
protein tyrosine kinase activity9
Annotation ExtensionEvidenceWith/FromReference
  • directly negatively regulates cdc2
  • has substrate wee1
GO Biological Process
Term NameCount
mitotic cytokinesis checkpoint5
Annotation ExtensionEvidenceWith/FromReference
mitotic G2 DNA damage checkpoint12
Annotation ExtensionEvidenceWith/FromReference
negative regulation of G2/M transition of mitotic cell cycle46
Annotation ExtensionEvidenceWith/FromReference
negative regulation of meiotic nuclear division10
Annotation ExtensionEvidenceWith/FromReference
regulation of G2/M transition of mitotic cell cycle73
Annotation ExtensionEvidenceWith/FromReference
signal transduction involved in G2 cell size control checkpoint1
Annotation ExtensionEvidenceWith/FromReference
signal transduction involved in mitotic DNA damage checkpoint8
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
medial cortical node12
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle pole body211
Annotation ExtensionEvidenceWith/FromReference
nucleoplasm256
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable


Population Phenotype

Term NameGenotypesCount
abolished cell division timing change upon nitrogen source shiftwee1-501
decreased bipolar indexwee1Δ27
decreased cell population growth during glucose starvationwee1Δ16
decreased cell population growth on glucose carbon sourcewee1Δ393
increased monopolar indexwee1Δ39
increased septation indexwee1-5066
inviable vegetative cell populationwee1-501468
wee1+ (wild type)
loss of viability upon glucose starvationwee1Δ9
sensitive to camptothecinwee1Δ259
sensitive to hydroxyureawee1Δ595
sensitive to ionizing radiation during vegetative growthwee1-5076
sensitive to methyl methanesulfonatewee1-50333
sensitive to miconazolewee1+ (wild type)40
sensitive to UV during vegetative growthwee1Δ161
viable vegetative cell populationwee1-503862
wee1Δ

Cell Phenotype

Term NameGenotypesCount
abnormal cell cycle arrest in mitotic G2 phase during glucose starvationwee1-501
wee1Δ
abnormal mitotic cell cycle arrest in response to nitrogen starvationwee1-5045
abnormal mitotic cell cycle regulation during cellular response to gamma radiationwee1-509
abnormal mitotic sister chromatid segregationwee1-50287
abnormal regulation of mitotic DNA replication initiationwee1-5021
abnormally arrested mitotic cell cycle progressionwee1-50179
abolished protein kinase activityK596L (K596L)29
increased duration of mitotic anaphasewee1+ (wild type)9
increased RNA level during nitrogen starvation22
affecting ste11wee1-50
increased RNA level during vegetative growth356
affecting ste11wee1-50
inviable elongated vegetative cellwee1+ (wild type)440
wee1-50
long mitotic spindlewee1+ (wild type)24
mitotic G1/S transition delaywee1-5014
normal cell cycle regulation during cellular response to hydroxyureawee1-5020
normal duration of mitotic anaphasewee1-502
normal nucleus:cytoplasm ratiowee1-506
normal protein localization to actomyosin contractile ring18
affecting nod1wee1-50
normal protein localization to medial cortex during vegetative growth16
affecting nod1wee1-50
normal protein localization to nucleus during vegetative growth85
affecting cdc25wee1+ (wild type)
normal protein phosphorylation during vegetative growth97
affecting cdc2wee1-50
normal protein tyrosine phosphorylation during vegetative growth8
affecting cdc2wee1Δ
affecting cdc2wee1-50
normal S-phase DNA damage checkpointwee1-5018
normal vegetative cell sizewee1-50439
premature mitotic G2/M transitionwee1-505
small nucleuswee1-501
small vegetative cellwee1-5090
viable elongated vegetative cell, elongated upon mitotic entrywee1+ (wild type)13
viable small vegetative cellwee-11556
wee1-1
wee1-8
wee1Δ
wee1-50
wee1-6

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
abolished cell population growth at high temperaturecdc2-3w (C67Y), wee1-50
wee1-50, ppa2Δ
decreased cell population growth at high temperaturegrt1+, slp1-362 (AA376->stop), wee1-50
decreased sporulation frequencywee1-50, mik1Δ
decreased vegetative cell population growthswo1-26 (G84C), wee1+ (wild type)
slx8-29, wee1-50
wee1-50, mcl1-1 (ntG372A)
skb1+, wee1Δ
swo1-26 (G84C), wee1-50
shk1+, wee1Δ
cdc2-4w (G200T), wee1-50
skb1+, wee1-50
inviable cell populationcsn1Δ, wee1-50, mik1Δ
inviable vegetative cell populationsrw1Δ, wee1-50
dis2+, cdc25-22 (C532Y), wee1-6
dis3+, cdc25-22 (C532Y), wee1-50
sds21+, cdc25-22 (C532Y), wee1-50
wee1-50, pin1Δ
cdc2-3w (C67Y), wis1Δ, wee1-50
pyp3Δ, cdc25Δ, wee1-50
cdc25-22 (C532Y), mcs1-77, wee1-50
win1-1, cdc25-22 (C532Y), wee1-50
dis2+, cdc25-22 (C532Y), wee1-50
cdc2-3w (C67Y), wee1-50
cdc2-3w (C67Y), sty1Δ, wee1-50
cdc25-22 (C532Y), mcs4-13, wee1-50
chk1::ura4 (deleted kinase domain), wee1-50
rad1-1, wee1-50
loss of viability at high temperaturewee1-50, mik1Δ
win1-1, cdc25-22 (C532Y), wee1-50
wee1-50, mcl1-1 (ntG372A)
resistance to Cutin-1wee1-50, cdc25-22 (C532Y)
sensitive to camptothecincdc2-1w (G146D), wee1Δ
sensitive to hydroxyureaslx8-29, wee1-50
cdc2-1w (G146D), wee1Δ
sensitive to methyl methanesulfonatemik1Δ, wee1-50
sensitive to UV during vegetative growthcdc2-1w (G146D), wee1Δ
viable vegetative cell populationcdc2+, wee1-50, mcs4-13, cdc25-22 (C532Y)
sds22+, cdc25-22 (C532Y), wee1-50
cdc25-22 (C532Y), mcs4-13, wee1-50
sds23+, cdc25-22 (C532Y), wee1-50
rad1-136, srw1+, wee1-50
cdc25-22 (C532Y), mcs1-77, wee1-50
sds21+, cdc25-22 (C532Y), wee1-50
cdc2-3w (C67Y), mcs4Δ, wee1-50
cdc25-22 (C532Y), wee1Δ
cdc14+, wee1-6
wee1-50, pyp3+, cdc25::ura4
dis3+, cdc25-22 (C532Y), wee1-50
wee1+ (wild type), cdc2-3w (C67Y)
wee1-50, cdc25-22 (C532Y), ppa2+
dis2+, cdc25-22 (C532Y), wee1-6
dis2+, cdc25-22 (C532Y), wee1-50

Cell Phenotype

Term NameGenotypes
abnormal cell separation after cytokinesis, with abnormal nuclear divisionchk1::ura4 (deleted kinase domain), wee1-50
abnormal cell sizewee1-50, cdc25-22 (C532Y)
abnormal chromosome segregationwee1-50, cdc25-22 (C532Y)
abnormal mitotic cell cycle regulation during cellular response to hydroxyureawee1::ura4+, cut5-T401
wee1+ (wild type), cut5-T401
wee1-50, cut5-T401
abnormal nucleuswee1-50, cdc25-22 (C532Y)
abnormal protein phosphorylation during vegetative growth
affecting cdc2cdc2-1w (G146D), wee1Δ
abnormal sporulation resulting in formation of two-spore ascuswin1-1, cdc25-22 (C532Y), wee1-50
cdc25-22 (C532Y), wee1-50
abolished mitotic G2 DNA damage checkpointwee1-50, chk1Δ, mik1Δ
abolished protein phosphorylation during meiotic cell cycle
affecting cdc2wee1-50, mei4Δ, mik1Δ
affecting cdc2wee1-50, mik1Δ
abolished sporulationwee1-50, mei4Δ, mik1Δ
altered DNA level during vegetative growthcdc2-3w (C67Y), wee1-50
cutwee1-50, orp1-4
cut during cellular response to hydroxyureawee1+ (wild type), cdc2-3w (C67Y)
decreased protein kinase activitycdc2-E8 (D213N), wee1-50
decreased protein level during vegetative growth
affecting wee1wee1 (wild type), wee1Δ
affecting wee1swo1-26 (G84C), wee1-50
decreased RNA level during meiotic cell cycle
affecting spo6wee1-50, mei4Δ, mik1Δ
elongated vegetative cellcdc2-3w (C67Y), mcs2-75, wee1-50
cdc2-3w (C67Y), mcs6-13 (L238R), wee1-50
increased cellular HMW SUMO conjugate levelslx8-29, wee1-50
increased protein level
affecting wee1wee1-50, orp1-4
increased SUMO chain lengthslx8-29, wee1-50
inviable after spore germination, without cell division, elongated cellpyp3Δ, cdc25Δ, wee1-50
inviable elongated mononucleate aseptate cellcdc2-E8 (D213N), wee1-50
inviable elongated mononucleate aseptate vegetative cell with cell cycle arrest in mitotic G2 phasesrw1Δ, wee1-50
wee1-50, srw1+, mik1Δ
inviable elongated multiseptate vegetative cellcdr1-76, wee1-50
cdr2-97, wee1-50
inviable elongated vegetative cellwin1-1, cdc25-22 (C532Y), wee1-50
win1-1, cdc25-22 (C532Y), wee1-6
chk1+ (wild type), wee1Δ
inviable septated mononucleate vegetative cellcdc2-3w (C67Y), wee1-50
large and small daughter nucleicdc2-3w (C67Y), wee1-50
mitotic catastrophesrw1Δ, cdc13+, wee1-50
rad1-1, wee1-50
rad1-136, wee1-50
pyp3+, wee1-50
wee1-50, srw1Δ
mitotic catastrophe with cutsrw1Δ, wee1-50
mitotic catastrophe with cut, small cellhus2-22 (W789->stop), wee1-50
cdc2-3w (C67Y), wee1-50
wee1-50, hus5.17 (G253T)
wee1-50, mik1Δ
wee1-50, rad1-h24
rad17-h11, wee1-50
rad3-h1, wee1-50
hus1-14, wee1-50
monoseptate vegetative cell with binucleate and anucleate compartmentssrw1Δ, wee1-50
normal cell cycle regulation during cellular response to hydroxyureacdc25Δ, wee1-50
wee1-50, mik1Δ
wee1+ (wild type), cdc2-3w (C67Y)
normal horsetail movementwee1-50, mik1Δ
normal S-phase DNA damage checkpointwee1-50, mik1Δ
premature mitosischk1::ura4 (deleted kinase domain), wee1-50
premature mitotic G2/M transitionatf1+, wee1-50
small vegetative cellsrk1Δ, wee1-50
alp4-C(566-784) (1-565), wee1-50
viable cell at high temperaturecdc2-A21 (D90N), cdc25+ (wild type), wee1-50
cdc2-E9 (D242N), cdc25+ (wild type), wee1-50
cdc2-E8 (D213N), cdc25+ (wild type), wee1-50
viable elongated vegetative cellwin1-1, cdc25-22 (C532Y), wee1-50
win1-1, cdc25-22 (C532Y), wee1-6
wee1-50, win1-1
rad1-136, srw1+, wee1-50
wee1+ (wild type), cdc2-3w (C67Y)
wee1-50, cdc25-22 (C532Y)
viable small vegetative cellwee1-50, wee-1
wee1-50, wee1-3
chk1::ura4 (deleted kinase domain), wee1-50
wee1-50, win1-1
cdr2-97, wee1-50
cdc2-3w (C67Y), mcs1-77, wee1-50
wee1-50, cdc25-22 (C532Y)
cdc2-3w (C67Y), mcs4-13, wee1-50
wee1-50, wee1+ (wild type)
ade6-704, wee1-112 (opal)
wee1+ (wild type), wee1-1
wee1-6, wee1+ (wild type)
orb6+, wee1-50
wee1-6, win1-1
dnt1Δ, wee1-50
wee1 (wild type), wee1Δ
wee1-50, wee-6
wee1+ (wild type), wee1-112 (opal)
wee-112 (opal), wee1-50
wee1-6, cdc25-22 (C532Y)
wee1-3, wee1+ (wild type)
cdr1-76, wee1-50
viable spheroid vegetative cellorb6-25, wee1-50
viable vegetative cellrad1-136, srw1+, wee1-50
viable vegetative cell with normal cell sizesup3-5 (G35C), wee1-112 (opal), ade6-704
cdr1-76, wee1-50
cdc25-22 (C532Y), wee1-50
cdr2-97, wee1-50
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in cdc45 DNA replication pre-initiation complex subunit Cdc45
FYPO affected by mutation in cdr1 NIM1 family serine/threonine protein kinase Cdr1/Nim1
FYPO affected by mutation in cds1 replication checkpoint kinase Cds1
FYPO affected by mutation in dnt1 nucleolar protein Dnt1
FYPO affected by mutation in hsp90 Hsp90 chaperone
FYPO affected by mutation in mrc1 claspin, Mrc1
FYPO affected by mutation in orc1 origin recognition complex subunit Orc1
FYPO affected by mutation in rad3 ATR checkpoint kinase Rad3
FYPO affected by mutation in rpn12 19S proteasome regulatory subunit Rpn12
FYPO affected by mutation in skp1 SCF ubiquitin ligase complex subunit Skp1
FYPO affected by mutation in tel2 Tel2/Rad-5/Clk-2 family protein Tel2
FYPO affected by mutation in wee1 M phase inhibitor protein kinase Wee1
GO localized by cdr2 serine/threonine protein kinase Cdr2
GO substrate of cdr2 serine/threonine protein kinase Cdr2
GO substrate of cds1 replication checkpoint kinase Cds1
GO substrate of pyp1 tyrosine phosphatase Pyp1
GO substrate of wee1 M phase inhibitor protein kinase Wee1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons721788..725835
mRNA721788..725835
5' UTR721788..722233PMID:21511999
CDS722234..724867
3' UTR724868..725835PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 567 837 108
SM00220 SMART IPR000719 Protein kinase domain 566 840 107
PS50011 Prosite Profiles IPR000719 Protein kinase domain 566 843 108
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 689 701 91
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 572 597 70
PTHR11042 HMMPANTHER 556 784 5
PTHR11042:SF95 HMMPANTHER 133 210 1
PTHR11042:SF95 HMMPANTHER 556 784 1
PTHR11042:SF95 HMMPANTHER 801 877 1
PTHR11042 HMMPANTHER 133 210 5
PTHR11042 HMMPANTHER 801 877 5
PTHR11042 HMMPANTHER 355 446 5
PTHR11042:SF95 HMMPANTHER 355 446 1
3.30.200.20 Gene3D Immunoglobulin-like fold 563 645 111
1.10.510.10 Gene3D Immunoglobulin-like fold 646 836 112
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 545 836 127

View domain organization at Pfam

Protein Properties

Ave. residue weight 109.76 Da
Charge 28.00
Codon Adaptation Index 0.44
Isoelectric point 10.00
Molecular weight 96.26 kDa
Number of residues 877
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2293
added by wee1
S788
Annotation ExtensionEvidenceResidueReference
added by wee1 IDA PMID:1825699
added by wee1 IDA PMID:7681363
added by wee1 IDA PMID:8256510
IDA S788 PMID:25720772
IDA PMID:1372994
O-phospho-L-threonine 1087
Annotation ExtensionEvidenceResidueReference
IDA PMID:1372994
O4'-phospho-L-tyrosine 281
added by wee1
removed by pyp1
Annotation ExtensionEvidenceResidueReference
added by wee1 IDA PMID:8256510
added by wee1 IDA PMID:7681363
added by wee1 IDA PMID:1825699
removed by pyp1 IDA PMID:8256510
IDA PMID:1372994
phosphorylated residue 2515
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
O-phosphorylated residueS777,S781 2461
Annotation ExtensionEvidenceResidueReference
IDA S777,S781 PMID:25720772
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0034599expression microarray evidencePMID:12529438

Quantitative Gene Expression

View graphical display of gene expression data for wee1 (SPCC18B5.03)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.8during GO:0072690PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
trichostatin A delays mitotic entry in cdc25-22 mutant PMID:197238881
trichostatin A partially suppresses wee1delta sensitivity to UV and hydroxyurea PMID:197238881
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3087
conserved in fungi4608
conserved in eukaryotes4516
conserved in metazoa3498
conserved in vertebrates3473
conserved in eukaryotes only2525
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPCC18B5.03 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captureshsp90Hsp90 chaperone Affinity Capture-WesternPMID:7813446
affinity capturescds1replication checkpoint kinase Cds1 Affinity Capture-WesternPMID:9572736
affinity captureswos2p23 homolog, predicted co-chaperone Wos2 Affinity Capture-WesternPMID:11298745
forms complex withsuc1cyclin-dependent protein kinase regulatory subunit Suc1 Reconstituted ComplexPMID:1372994
binds DNA-binding domain construct withskb1type II protein arginine N-methyltransferase Skb1 Two-hybridPMID:23615447
binds DNA-binding domain construct withcdr2serine/threonine protein kinase Cdr2 Two-hybridPMID:26071525
binds DNA-binding domain construct withcdr1NIM1 family serine/threonine protein kinase Cdr1/Nim1 Two-hybridPMID:23615447
modifiescds1replication checkpoint kinase Cds1 Biochemical ActivityPMID:11606526
modifiescdc2cyclin-dependent protein kinase Cdk1/Cdc2 Biochemical ActivityPMID:1850698
modified bypyp2tyrosine phosphatase Pyp2 Biochemical ActivityPMID:8256510
modified bypyp1tyrosine phosphatase Pyp1 Biochemical ActivityPMID:8256510
modified bycds1replication checkpoint kinase Cds1 Biochemical ActivityPMID:21099360
modified bychk1Chk1 protein kinase Biochemical ActivityPMID:9034337
modified bycdr1NIM1 family serine/threonine protein kinase Cdr1/Nim1 Biochemical ActivityPMID:8515817
modified bycdr2serine/threonine protein kinase Cdr2 Biochemical ActivityPMID:9843572
affinity captured bycdr2serine/threonine protein kinase Cdr2 Affinity Capture-WesternPMID:26071525
affinity captured byskb1type II protein arginine N-methyltransferase Skb1 Affinity Capture-WesternPMID:23615447
affinity captured bycds1replication checkpoint kinase Cds1 Affinity Capture-WesternPMID:9572736
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPCC18B5.03 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
rescuespyp2tyrosine phosphatase Pyp2 Phenotypic SuppressionPMID:1448087
rescuesskb1type II protein arginine N-methyltransferase Skb1 Phenotypic SuppressionPMID:23615447
rescuesslp1substrate-specific mitotic metaphase/anaphase APC coactivator Slp1 Phenotypic SuppressionPMID:9001228
rescueswis1MAP kinase kinase Wis1 Phenotypic SuppressionPMID:1756736
rescuespof3F-box protein Pof3 Phenotypic SuppressionPMID:11809834
rescuesbgs1primary septum and spore wall linear 1,3-beta-glucan synthase catalytic subunit Bgs1 Phenotypic SuppressionPMID:10704373
rescuescdc16two-component GAP Cdc16 Phenotypic SuppressionPMID:20805322
rescuespat1serine/threonine protein kinase Ran1/Pat1 Phenotypic SuppressionPMID:10521402
rescuesSPAC144.17c6-phosphofructo-2-kinase (predicted) Phenotypic SuppressionPMID:17900713
rescuescdr2serine/threonine protein kinase Cdr2 Phenotypic SuppressionPMID:9843572
rescuesslm9hira protein Slm9 Phenotypic SuppressionPMID:10835386
rescuespab1protein phosphatase PP2A regulatory subunit B-55 Pab1 Phenotypic SuppressionPMID:20876564
rescuesini1RING finger-like protein Ini1 Phenotypic SuppressionPMID:14762117
rescueshob1BAR adaptor protein Hob1 Phenotypic SuppressionPMID:12569356
rescuescdc25M phase inducer tyrosine phosphatase Cdc25 Phenotypic SuppressionPMID:3442828
rescuesclp1Cdc14-related protein phosphatase Clp1/Flp1 Phenotypic SuppressionPMID:11683392
rescuesspo5meiotic RNA-binding protein 1 Phenotypic SuppressionPMID:24506453
rescuesssp1Ca2+/calmodulin-dependent (CaMMK)-like protein kinase Ssp1 Phenotypic SuppressionPMID:10233158
rescuescdc3profilin Phenotypic SuppressionPMID:20805322
rescuespyp1tyrosine phosphatase Pyp1 Phenotypic SuppressionPMID:1448087
rescuesfin1serine/threonine protein kinase, NIMA related Fin1 Phenotypic SuppressionPMID:12065422
rescuesatf1transcription factor, Atf-CREB family Atf1 Phenotypic SuppressionPMID:9135083
rescuescdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic SuppressionPMID:3032459
rescuesfkh2forkhead transcription factor Fkh2 Phenotypic SuppressionPMID:22451489
enhances phenotype ofmed8mediator complex subunit Med8 Phenotypic EnhancementPMID:10392445
enhances phenotype ofcdc25M phase inducer tyrosine phosphatase Cdc25 Phenotypic EnhancementPMID:10233158
enhances phenotype ofsep1forkhead transcription factor Sep1 Phenotypic EnhancementPMID:10392445
enhances phenotype ofpcp1pericentrin Pcp1 Phenotypic EnhancementPMID:19942852
enhances phenotype ofclp1Cdc14-related protein phosphatase Clp1/Flp1 Phenotypic EnhancementPMID:11683392
enhances phenotype ofcdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic EnhancementPMID:9013342
enhances phenotype ofspt8SAGA complex subunit Spt8 Phenotypic EnhancementPMID:10392445
enhances phenotype ofbgs1primary septum and spore wall linear 1,3-beta-glucan synthase catalytic subunit Bgs1 Phenotypic EnhancementPMID:10503548
enhances phenotype ofliz1pantothenate transmembrane transporter Liz1 Phenotypic EnhancementPMID:9950674
enhances phenotype oforc1origin recognition complex subunit Orc1 Phenotypic EnhancementPMID:12186947
enhances phenotype ofzfs1CCCH tandem zinc finger protein, human Tristetraprolin homolog Zfs1, involved in mRNA catabolism Phenotypic EnhancementPMID:10462526
enhances phenotype ofreb1RNA polymerase I transcription termination factor/ RNA polymerase II transcription factor Reb1 Phenotypic EnhancementPMID:21118960
enhances phenotype ofrad31SUMO activating enzyme E1-type Rad31 Phenotypic EnhancementPMID:9092625
enhances phenotype ofsrk1MAPK-activated protein kinase Srk1 Phenotypic EnhancementPMID:15629716
phenotype enhanced byrum1CDK inhibitor Rum1 Phenotypic EnhancementPMID:9013342
phenotype enhanced bycdc25M phase inducer tyrosine phosphatase Cdc25 Phenotypic EnhancementPMID:9013342
phenotype enhanced bymik1mitotic inhibitor kinase Mik1 Phenotypic EnhancementPMID:10947840
phenotype enhanced bychk1Chk1 protein kinase Phenotypic EnhancementPMID:10637286
phenotype enhanced bywin1MAP kinase kinase kinase Win1 Phenotypic EnhancementPMID:3442828
phenotype enhanced bycdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic EnhancementPMID:3116001
phenotype enhanced byklp5kinesin-8 family plus-end microtubule motor Klp5 Phenotypic EnhancementPMID:11739790
phenotype enhanced byklp6kinesin-8 family plus-end microtubule motor Klp6 Phenotypic EnhancementPMID:11739790
phenotype enhanced bymis3rRNA processing protein Mis3 Phenotypic EnhancementPMID:10947840
synthetic lethal withmor2morphogenesis protein Mor2 Synthetic LethalityPMID:12234926
synthetic lethal withmik1mitotic inhibitor kinase Mik1 Synthetic LethalityPMID:11809834
synthetic lethal withcdc2cyclin-dependent protein kinase Cdk1/Cdc2 Synthetic LethalityPMID:2474475
synthetic lethal withrad1checkpoint clamp complex protein Rad1 Synthetic LethalityPMID:8590806
synthetic lethal withsrw1substrate-specific mitotic G1 APC coactivator Srw1/Ste9 Synthetic LethalityPMID:9398669
synthetic lethal withpal1membrane associated protein Pal1 Synthetic LethalityPMID:15975911
synthetic lethal withchk1Chk1 protein kinase Synthetic LethalityPMID:11270571
synthetic lethal withppa2serine/threonine protein phosphatase Ppa2 Synthetic LethalityPMID:8389306
synthetic lethal withrad3ATR checkpoint kinase Rad3 Synthetic LethalityPMID:9398669
synthetic lethal withreb1RNA polymerase I transcription termination factor/ RNA polymerase II transcription factor Reb1 Synthetic LethalityPMID:21118960
synthetic lethal withpub1HECT-type ubiquitin-protein ligase E3 Pub1 Synthetic LethalityPMID:8635463
synthetic lethal withtor1serine/threonine protein kinase Tor1 Synthetic LethalityPMID:19546237
synthetic lethal withhus5SUMO conjugating enzyme E2 Hus5 Synthetic LethalityPMID:1427071
synthetic lethal withams2cell cycle regulated GATA-type transcription factor Ams2 Synthetic LethalityPMID:18077559
synthetic lethal withhus1checkpoint clamp complex protein Hus1 Synthetic LethalityPMID:1427071
synthetic lethal withrad17RFC related checkpoint protein Rad17 Synthetic LethalityPMID:1427071
synthetic lethal withrqh1RecQ type DNA helicase Rqh1 Synthetic LethalityPMID:1427071
synthetically rescued byres2MBF transcription factor complex subunit Res2 Synthetic RescuePMID:11029045
synthetically rescued bymcs4response regulator Mcs4 Synthetic RescuePMID:9383051
synthetically rescued bycig2G1/S-specific B-type cyclin Cig2 Synthetic RescuePMID:8455610
synthetically rescued bycdc2cyclin-dependent protein kinase Cdk1/Cdc2 Synthetic RescuePMID:24861625
synthetically rescued bypyp1tyrosine phosphatase Pyp1 Synthetic RescuePMID:1464319
synthetically rescued byhsp90Hsp90 chaperone Synthetic RescuePMID:7813446
rescued bycig2G1/S-specific B-type cyclin Cig2 Phenotypic SuppressionPMID:8972180
rescued bycdc25M phase inducer tyrosine phosphatase Cdc25 Phenotypic SuppressionPMID:9473044
rescued bySPAC29A4.02ctranslation elongation factor EF-1 gamma subunit Phenotypic SuppressionPMID:14985109
rescued byyox1MBF complex corepressor Yox1 Phenotypic SuppressionPMID:21132016
rescued bytor1serine/threonine protein kinase Tor1 Phenotypic SuppressionPMID:19546237
rescued bywin1MAP kinase kinase kinase Win1 Phenotypic SuppressionPMID:3442828
rescued bymug134endosulfine Igo1 Phenotypic SuppressionPMID:26776736
rescued by overexpression ofsrw1substrate-specific mitotic G1 APC coactivator Srw1/Ste9 Dosage RescuePMID:9398669
rescued by overexpression ofdis2serine/threonine protein phosphatase PP1, Dis2 Dosage RescuePMID:1944266
rescued by overexpression ofcdc14SIN component Cdc14 Dosage RescuePMID:24006256
rescued by overexpression ofswr1SNF2 family ATP-dependent DNA helicase Swr1 Dosage RescuePMID:9571240
rescued by overexpression ofsds21serine/threonine protein phosphatase PP1 subfamily, Sds21 Dosage RescuePMID:1944266
rescued by overexpression ofrum1CDK inhibitor Rum1 Dosage RescuePMID:9571240
rescued by overexpression ofdis33'-5' exoribonuclease subunit Dis3 Dosage RescuePMID:1944266
rescued by overexpression ofwis1MAP kinase kinase Wis1 Dosage RescuePMID:1756736
rescued by overexpression ofmik1mitotic inhibitor kinase Mik1 Dosage RescuePMID:1706223
inviable in presence of overexpressedshk1PAK-related kinase Shk1 Dosage LethalityPMID:9843966
inviable in presence of overexpressedcdc13G2/M B-type cyclin Cdc13 Dosage LethalityPMID:9398669
inviable in presence of overexpressedskb1type II protein arginine N-methyltransferase Skb1 Dosage LethalityPMID:9843966
synthetic growth defect withcdc25M phase inducer tyrosine phosphatase Cdc25 Synthetic Growth DefectPMID:23333317
synthetic growth defect withcdc2cyclin-dependent protein kinase Cdk1/Cdc2 Synthetic Growth DefectPMID:11029045
synthetic growth defect withcdc28ATP-dependent RNA helicase Cdc28 Synthetic Growth DefectPMID:16049013
synthetic growth defect withcrb2DNA repair protein Rad9 homolog Crb2 Synthetic Growth DefectPMID:20679488
synthetic growth defect withslm9hira protein Slm9 Synthetic Growth DefectPMID:9843572
synthetic growth defect withhus5SUMO conjugating enzyme E2 Hus5 Synthetic Growth DefectPMID:7768995
synthetic growth defect withmcl1DNA polymerase alpha accessory factor Mcl1 Synthetic Growth DefectPMID:12455694
synthetic growth defect withpin1peptidyl-prolyl cis-trans isomerase Pin1 Synthetic Growth DefectPMID:11707530
synthetic growth defect withprp12U2 snRNP-associated protein Sap130 Synthetic Growth DefectPMID:16049013
synthetic growth defect withdbr1RNA lariat debranching enzyme Dbr1 Synthetic Growth DefectPMID:16049013
synthetic growth defect withpcp1pericentrin Pcp1 Synthetic Growth DefectPMID:21233285
synthetic growth defect withmik1mitotic inhibitor kinase Mik1 Synthetic Growth DefectPMID:11029045
synthetically rescuescdc25M phase inducer tyrosine phosphatase Cdc25 Synthetic RescuePMID:9843572
synthetically rescuesrqh1RecQ type DNA helicase Rqh1 Synthetic RescuePMID:26093291
synthetically rescuescdr1NIM1 family serine/threonine protein kinase Cdr1/Nim1 Synthetic RescuePMID:2245912
synthetically rescuessty1MAP kinase Sty1 Synthetic RescuePMID:7501024
synthetically rescuesslp1substrate-specific mitotic metaphase/anaphase APC coactivator Slp1 Synthetic RescuePMID:11058086
synthetically rescueschk1Chk1 protein kinase Synthetic RescuePMID:16816416
synthetically rescueshhp1serine/threonine protein kinase Hhp1 Synthetic RescuePMID:24861625
synthetically rescuescdr2serine/threonine protein kinase Cdr2 Synthetic RescuePMID:9843572
synthetically rescuespot1telomere end-binding protein Pot1 Synthetic RescuePMID:26093291
synthetically rescuesmei4meiotic forkhead transcription factor Mei4 Synthetic RescuePMID:25492408
growth defect in presence of overexpressedatf1transcription factor, Atf-CREB family Atf1 Dosage Growth DefectPMID:24728197
overexpression rescuescdc2cyclin-dependent protein kinase Cdk1/Cdc2 Dosage RescuePMID:1427071
External References
Database Identifier Description
NBRP SPCC18B5.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC18B5.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC18B5.03 BioGRID Interaction Datasets
Expression Viewer SPCC18B5.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC18B5.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC18B5.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC18B5.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC18B5.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC18B5.03 Transcriptome Viewer (Bähler Lab)
GEO SPCC18B5.03 GEO profiles
PInt SPCC18B5.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC18B5.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC18B5.03 Fission yeast phenotypic data & analysis
Cyclebase SPCC18B5.03.1 Cell Cycle Data
SPD / RIKEN47/47A06Orfeome Localization Data
UniProtKB/SwissProtP07527Mitosis inhibitor protein kinase wee1
ModBaseP07527Database of comparative protein structure models
STRINGP07527Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587933M phase inhibitor protein kinase Wee1
RefSeq mRNANM_001022924972h- M phase inhibitor protein kinase Wee1 (wee1), mRNA
European Nucleotide ArchiveAAA35354.1ENA Protein Mapping
European Nucleotide ArchiveBAA87204.1ENA Protein Mapping
European Nucleotide ArchiveCAB52150.1ENA Protein Mapping
UniParcUPI0000138EF6UniProt Archive

Literature for wee1

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Release Version: PomBase:30_62 - 30 Jan 2017