myo52 (SPCC1919.10c)


Gene Standard Namemyo52 Characterisation Statuspublished
Systematic IDSPCC1919.10c Feature Typeprotein coding
Synonyms Name Description
Productmyosin type V Product Size1516aa, 175.16 kDa
Genomic Location Chromosome III, 2229932-2224882 (5051nt); CDS:2229731-2225048 (4684nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
actin binding38
Annotation ExtensionEvidenceWith/FromReference
ATP binding559
Annotation ExtensionEvidenceWith/FromReference
calmodulin binding13
Annotation ExtensionEvidenceWith/FromReference
microfilament motor activity4
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
actin filament bundle organization15
Annotation ExtensionEvidenceWith/FromReference
actin filament bundle retrograde transport1
Annotation ExtensionEvidenceWith/FromReference
establishment or maintenance of cell polarity regulating cell shape55
Annotation ExtensionEvidenceWith/FromReference
formin-nucleated actin cable assembly2
Annotation ExtensionEvidenceWith/FromReference
mitotic cytokinesis106
Annotation ExtensionEvidenceWith/FromReference
protein localization to actin fusion focus2
Annotation ExtensionEvidenceWith/FromReference
protein localization to cell tip3
Annotation ExtensionEvidenceWith/FromReference
vesicle transport along actin filament1
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actin cortical patch47
Annotation ExtensionEvidenceWith/FromReference
actin fusion focus13
Annotation ExtensionEvidenceWith/FromReference
cell cortex of cell tip44
Annotation ExtensionEvidenceWith/FromReference
cell cortex of growing cell tip8
Annotation ExtensionEvidenceWith/FromReference
cell division site309
Annotation ExtensionEvidenceWith/FromReference
cell tip200
Annotation ExtensionEvidenceWith/FromReference
lateral cell cortex6
Annotation ExtensionEvidenceWith/FromReference
medial cortex33
Annotation ExtensionEvidenceWith/FromReference
myosin complex8
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viability depends on conditions

Single-allele Phenotypes

Population Phenotype

Term NameGenotypesCount
abolished cell population growth at high temperatureATPase dead mutant20
C-terminal truncation (1320-1516)
myo52Δ
C-terminal truncation 940-1516 (940-1516)
decreased cell population growth on glucose carbon sourcemyo52Δ286
inviable vegetative cell populationmyo52+ (wild type)1444
myo52Δ
sensitive to 5-fluorouracilmyo52Δ272
sensitive to rapamycinmyo52Δ78
viable vegetative cell populationmyo52Δ3819

Cell Phenotype

Term NameGenotypesCount
abnormal actin cable organizationmyo52Δ5
abnormal protein localization during vegetative growth367
affecting myo52ATPase dead mutant
affecting myo52C-terminal truncation 940-1516 (940-1516)
abnormal protein localization to shmoo tip2
affecting fus1myo52Δ
abolished protein localization to cell division site33
affecting bgs1C-terminal truncation (1320-1516)
abolished protein localization to cell tip29
affecting sec3, exo70 and sec5myo52Δ
affecting bgs1myo52Δ
abolished protein localization to cell tip, with protein mislocalized to cytoplasm10
affecting bgs1C-terminal truncation (1320-1516)
decreased protein level during vegetative growth102
affecting myo52Δ-tail (1162-1516)
decreased protein localization to actomyosin contractile ring14
affecting chs2myo52Δ
decreased protein localization to cell division site26
affecting bgs1myo52Δ
decreased protein localization to cell tip26
affecting mug33myo52Δ
decreased protein secretionmyo52Δ14
decreased rate of actin cable retrograde transportmyo52Δ2
dispersed actin cortical patch localizationmyo52Δ31
incomplete cell wall disassembly at cell fusion sitemyo52Δ1
loss of punctate cytoplasmic protein localization5
affecting myo52Δ-tail (1162-1516)
normal protein localization during vegetative growth219
affecting sec3myo52Δ
affecting myo52C-terminal truncation (1320-1516)
normal protein localization to cell division site32
affecting ags1myo52Δ
normal protein localization to cell tip23
affecting sec3, exo70 and sec5Tea2N-CFP-Myo52C (fusion of Tea2 N-terminal motor domain, CFP, and Myo52 C-terminal domain)
affecting ags1myo52Δ
affecting myo52Δ-tail (1162-1516)
affecting for3myo52Δ
normal rate of actomyosin contractile ring contractionmyo52Δ3
pear-shaped vegetative cellmyo52Δ16
short, misoriented actin cablesmyo52Δ2
viable spheroid vegetative cellmyo52Δ50
viable stubby vegetative cellC-terminal truncation 940-1516 (940-1516)73
C-terminal truncation (1320-1516)
myo52Δ
Δ-tail (1162-1516)
ATPase dead mutant

Multi-allele phenotypes


Cell Phenotype

Term NameGenotypes
abnormal cell polaritygef3Δ, myo52Δ
abnormal endoplasmic reticulum localizationmyo52Δ, scs22Δ, myo51Δ, scs2Δ, for3Δ
abnormal protein localization to shmoo tip
affecting fus1myo52Δ, myo51Δ
abnormal septationgef3Δ, myo52Δ
abolished cell fusion during conjugationmyo52Δ, myo51Δ
stubby cellmyo52Δ, scs22Δ, myo51Δ, scs2Δ, for3Δ
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in for3 formin For3
FYPO affected by mutation in mas5 DNAJ domain protein Mas5 (predicted)
FYPO affected by mutation in myo52 myosin type V
GO localized by orb6 serine/threonine protein kinase Orb6
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2229932..2229703, 2229643..2229535, 2229460..2224882
Intron2229702..2229644, 2229534..2229461
mRNA2229932..2224882
5' UTR2229932..2229732PMID:21511999
CDS2229731..2229703, 2229643..2229535, 2229460..2225048
3' UTR2225047..2224882SPD250
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 842 859 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 892 908 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 866 886 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 820 837 3
PF00063 Pfam IPR001609 Myosin head, motor domain 76 754 5
PF01843 Pfam IPR002710 Dilute domain 1313 1410 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 791 813 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 816 838 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 887 909 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 839 861 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 864 886 3
SM00242 SMART IPR001609 Myosin head, motor domain 67 767 5
PS51456 Prosite Profiles IPR001609 Myosin head, motor domain 73 766 5
PS51126 Prosite Profiles IPR002710 Dilute domain 1163 1431 3
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 840 869 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 888 917 5
PTHR13140:SF507 HMMPANTHER 1429 1440 1
PTHR13140 HMMPANTHER 813 960 8
PTHR13140 HMMPANTHER 10 764 8
PTHR13140 HMMPANTHER 988 1043 8
PTHR13140 HMMPANTHER 1429 1440 8
PTHR13140:SF507 HMMPANTHER 10 764 1
PTHR13140:SF507 HMMPANTHER 988 1043 1
PTHR13140:SF507 HMMPANTHER 813 960 1
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 156 186 288
1.20.5.190 Gene3D P-loop containing nucleoside triphosphate hydrolase 839 876 2
1.20.5.190 Gene3D P-loop containing nucleoside triphosphate hydrolase 797 836 2
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 819 914 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 12 811 294
Coil ncoils Predicted coiled-coil protein (DUF2205) 990 1024 1048
Coil ncoils Predicted coiled-coil protein (DUF2205) 932 973 1048
PR00193 PRINTS IPR001609 Myosin head, motor domain 505 533 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 216 243 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 452 480 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 103 122 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 160 185 5

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0001026IQ domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001026

Protein Properties

Ave. residue weight 115.54 Da
Charge 2.50
Isoelectric point 6.64
Molecular weight 175.16 kDa
Number of residues 1516
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1674
present during mitotic M phaseS1069
present during mitotic M phaseS1072
S399, S1072
present during mitotic M phaseS1065
present during mitotic M phaseS985
Annotation ExtensionEvidenceResidueReference
experimental evidence S399 PMID:24763107
present during mitotic M phase experimental evidence S985 PMID:21712547
present during mitotic M phase experimental evidence S1065 PMID:21712547
present during mitotic M phase experimental evidence S1069 PMID:21712547
experimental evidence S1072 PMID:24763107
present during mitotic M phase experimental evidence S1072 PMID:21712547
phosphorylated residue 1930
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3071during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2504during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2708during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2962during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1506.56during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
3082during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1428.57during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.7during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.55during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3421
conserved in vertebrates3396
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load gene that interact physically with SPCC1919.10c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byrng3UCS-domain protein Rng3 Affinity Capture-RNAPMID:19098712
affinity captured bysds23PP2A-type phosphatase inhibitor Sds23/Moc1 Affinity Capture-MSPMID:19371376
affinity captured bymyp2myosin II heavy chain Myo3 Affinity Capture-RNAPMID:19805578
affinity captured bymyo2myosin II heavy chain Affinity Capture-RNAPMID:19805578
affinity captured bymyo1myosin type I Affinity Capture-RNAPMID:19805578
affinity captured bymyo51myosin type V Affinity Capture-RNAPMID:19805578
affinity captured byfor3formin For3 Affinity Capture-WesternPMID:23051734
affinity capturesmyo2myosin II heavy chain Affinity Capture-RNAPMID:19805578
affinity capturesmyp2myosin II heavy chain Myo3 Affinity Capture-RNAPMID:19805578
affinity capturesfor3formin For3 Affinity Capture-WesternPMID:23051734
affinity capturesmyo52myosin type V Affinity Capture-WesternPMID:24196839
affinity capturestip1CLIP170 family protein Tip1 Affinity Capture-WesternPMID:19808886
affinity capturespkd2TRP-like ion channel Pkd2 Affinity Capture-WesternPMID:19543678
affinity capturesypt3GTPase Ypt3 Affinity Capture-WesternPMID:22137473
affinity capturesdph1UBA domain protein Dph1 Affinity Capture-WesternPMID:19808886
affinity capturesrng2IQGAP Affinity Capture-RNAPMID:19805578
affinity capturesmyo1myosin type I Affinity Capture-RNAPMID:19805578
forms complex withact1actin Act1 Reconstituted ComplexPMID:24196839
binds activation domain construct withcam1calmodulin Cam1 Two-hybridPMID:18003699
binds activation domain construct withdph1UBA domain protein Dph1 Two-hybridPMID:19808886
binds activation domain construct withmyo52myosin type V Two-hybridPMID:18003699
Genetic Interactions

Source: BioGRID

Load gene that interact geneitically with SPCC1919.10c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withexo70exocyst complex subunit Exo70 Synthetic Growth DefectPMID:21652630
synthetic growth defect withfor3formin For3 Synthetic Growth DefectPMID:21652630
synthetic growth defect withtea4tip elongation aberrant protein Tea4 Synthetic Growth DefectPMID:22137473
phenotype enhanced bysec8exocyst complex subunit Sec8 Phenotypic EnhancementPMID:21148300
phenotype enhanced byexo70exocyst complex subunit Exo70 Phenotypic EnhancementPMID:21148300
phenotype enhanced bymal3EB1 family Mal3 Phenotypic EnhancementPMID:23051734
rescueskin1microtubule affinity-regulating kinase Kin1 Phenotypic SuppressionPMID:23294323
rescuesfor3formin For3 Phenotypic SuppressionPMID:22137473
synthetic lethal withexo70exocyst complex subunit Exo70 Synthetic LethalityPMID:21148300
synthetic lethal withsec8exocyst complex subunit Sec8 Synthetic LethalityPMID:21148300
synthetic lethal withmug33Tea1-interacting protein involved in exocytosis Synthetic LethalityPMID:21652630
synthetic lethal withrho3Rho family GTPase Rho3 Synthetic LethalityPMID:21652630
enhances phenotype ofmyo51myosin type V Phenotypic EnhancementPMID:23051734
External References
Database Identifier Description
NBRP SPCC1919.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1919.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1919.10c BioGRID Interaction Datasets
Expression Viewer SPCC1919.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1919.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1919.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1919.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1919.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1919.10c Transcriptome Viewer (Bähler Lab)
GEO SPCC1919.10c GEO profiles
PInt SPCC1919.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC1919.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC1919.10c Fission yeast phenotypic data & analysis
Cyclebase SPCC1919.10c.1 Cell Cycle Data
SPD / RIKEN31/31A01Orfeome Localization Data
UniProtKB/SwissProtO94477Myosin-52
ModBaseO94477Database of comparative protein structure models
STRINGO94477Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588492myosin type V
RefSeq mRNANM_001023482972h- myosin type V (myo52), mRNA
European Nucleotide ArchiveAB028001ENA EMBL mapping
European Nucleotide ArchiveCU329672ENA EMBL mapping
European Nucleotide ArchiveD89250ENA EMBL mapping
European Nucleotide ArchiveBAA13911ENA Protein Mapping
European Nucleotide ArchiveBAA87305ENA Protein Mapping
European Nucleotide ArchiveBAA87305.1ENA Protein Mapping
European Nucleotide ArchiveCAA22641ENA Protein Mapping
European Nucleotide ArchiveCAA22641.1ENA Protein Mapping
UniParcUPI0000069836UniProt Archive

Literature for myo52

Search: Europe PMC or PubMed

Release Version: PomBase:29_56 - 12 Nov 2015