myo52 (SPCC1919.10c)


Gene Standard Namemyo52 Characterisation Statuspublished
Systematic IDSPCC1919.10c Feature Typeprotein coding
Synonyms Name Description
Productmyosin type V Product Size1516aa, 175.16 kDa
Genomic Location Chromosome III, 2229932-2224882 (5051nt); CDS:2229731-2225048 (4684nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
actin binding38
Annotation ExtensionEvidenceWith/FromReference
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
calmodulin binding10
Annotation ExtensionEvidenceWith/FromReference
microfilament motor activity4
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
actin filament bundle organization14
Annotation ExtensionEvidenceWith/FromReference
actin filament bundle retrograde transport1
Annotation ExtensionEvidenceWith/FromReference
cellular protein localization553
Annotation ExtensionEvidenceWith/FromReference
establishment or maintenance of cell polarity regulating cell shape57
Annotation ExtensionEvidenceWith/FromReference
formin-nucleated actin cable assembly2
Annotation ExtensionEvidenceWith/FromReference
mitotic cytokinesis121
Annotation ExtensionEvidenceWith/FromReference
protein localization to cell tip4
Annotation ExtensionEvidenceWith/FromReference
vesicle transport along actin filament1
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actin cortical patch47
Annotation ExtensionEvidenceWith/FromReference
cell cortex of cell tip44
Annotation ExtensionEvidenceWith/FromReference
cell cortex of growing cell tip8
Annotation ExtensionEvidenceWith/FromReference
cell division site305
Annotation ExtensionEvidenceWith/FromReference
cell tip194
Annotation ExtensionEvidenceWith/FromReference
lateral cell cortex6
Annotation ExtensionEvidenceWith/FromReference
medial cortex33
Annotation ExtensionEvidenceWith/FromReference
myosin complex8
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viability depends on conditions

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growth on glucose carbon sourcemyo52ΔNull247
inviable vegetative cell populationmyo52+ (wild type)Overexpression1428
myo52ΔNull
sensitive to 5-fluorouracilmyo52ΔNull271
sensitive to rapamycinmyo52ΔNull78
viable vegetative cell populationmyo52ΔNull3792

Cell Phenotype

Term NameAlleleExpressionCount
abnormal actin cable organizationmyo52ΔNull5
abolished protein localization to cell tip29
affecting sec3, exo70 and sec5myo52ΔNull
decreased protein level during vegetative growth91
affecting myo52Δ-tail (1162-1516)Not specified
decreased protein localization to actomyosin contractile ring11
affecting chs2myo52ΔNull
decreased protein localization to cell tip23
affecting mug33myo52ΔNull
decreased protein secretionmyo52ΔNull14
decreased rate of actin cable retrograde transportmyo52ΔNull2
dispersed actin cortical patch localizationmyo52ΔNull26
loss of punctate cytoplasmic protein localization5
affecting myo52Δ-tail (1162-1516)Not specified
normal protein localization during vegetative growth169
affecting sec3myo52ΔNull
normal protein localization to cell division site28
affecting ags1myo52ΔNull
normal protein localization to cell tip21
affecting myo52Δ-tail (1162-1516)Not specified
affecting ags1myo52ΔNull
affecting for3myo52ΔNull
affecting sec3, exo70 and sec5Tea2N-CFP-Myo52C (fusion of Tea2 N-terminal motor domain, CFP, and Myo52 C-terminal domain)Not specified
pear-shaped vegetative cellmyo52ΔNull33
short, misoriented actin cablesmyo52ΔNull2
viable spheroid vegetative cellmyo52ΔNull49
viable stubby vegetative cellΔ-tail (1162-1516)Not specified71
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in for3 formin For3 PMID:23051734
FYPO affected by mutation in mas5 DNAJ domain protein Mas5 (predicted) PMID:24146635
FYPO affected by mutation in myo52 myosin type V PMID:23051734
GO transported by orb6 serine/threonine protein kinase Orb6 PMID:19646873
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2229932..2229703, 2229643..2229535, 2229460..2224882
mRNA2229932..2224882
5' UTR2229932..2229732PMID:21511999
CDS2229731..2229703, 2229643..2229535, 2229460..2225048
3' UTR2225047..2224882SPD250
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00063 Pfam IPR001609 Myosin head, motor domain 76 754 5
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 866 886 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 842 858 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 892 908 3
PF01843 Pfam IPR018444 Dil domain 1313 1413 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 887 909 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 816 838 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 791 813 3
SM00242 SMART IPR001609 Myosin head, motor domain 67 767 5
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 864 886 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 839 861 3
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 888 917 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 840 869 5
PS51456 Prosite Profiles IPR001609 Myosin head, motor domain 73 766 5
PS51126 Prosite Profiles IPR002710 Dilute 1163 1431 3
PTHR13140 HMMPANTHER 1 1109 6
PTHR13140:SF191 HMMPANTHER 1 1109 1
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 156 186 288
1.20.5.190 Gene3D 797 836 2
1.20.5.190 Gene3D 839 876 2
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 12 811 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 819 914 294
Coil ncoils Rabaptin coiled-coil domain 932 974 968
Coil ncoils Rabaptin coiled-coil domain 990 1025 968
PR00193 PRINTS IPR001609 Myosin head, motor domain 160 185 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 103 122 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 452 480 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 216 243 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 505 533 5

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001026IQ domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001026

Protein Properties

Ave. residue weight 115.54 Da
Charge 2.50
Isoelectric point 6.64
Molecular weight 175.16 kDa
Number of residues 1516
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS399, S1072 1672
present during mitotic M phaseS1072
present during mitotic M phaseS1069
present during mitotic M phaseS1065
present during mitotic M phaseS985
Annotation ExtensionEvidenceResidueReference
experimental evidence S399 PMID:24763107
present during mitotic M phase experimental evidence S985 PMID:21712547
present during mitotic M phase experimental evidence S1065 PMID:21712547
present during mitotic M phase experimental evidence S1069 PMID:21712547
experimental evidence S1072 PMID:24763107
present during mitotic M phase experimental evidence S1072 PMID:21712547
phosphorylated residue 1926
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3071during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2504during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2708during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2962during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1506.56during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
3082during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1428.57during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.7during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.55during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
forms complex withact1actin Act1 Reconstituted ComplexPMID:24196839
binds activation domain construct withcam1calmodulin Cam1 Two-hybridPMID:18003699
affinity capturesdph1UBA domain protein Dph1 Affinity Capture-WesternPMID:19808886
binds activation domain construct withdph1UBA domain protein Dph1 Two-hybridPMID:19808886
affinity capturesfor3formin For3 Affinity Capture-WesternPMID:23051734
affinity captured byfor3formin For3 Affinity Capture-WesternPMID:23051734
affinity captured byhhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
affinity captured byhhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
affinity capturesmyo1myosin type I Affinity Capture-RNAPMID:19805578
affinity captured bymyo1myosin type I Affinity Capture-RNAPMID:19805578
affinity capturesmyo2myosin II heavy chain Affinity Capture-RNAPMID:19805578
affinity captured bymyo2myosin II heavy chain Affinity Capture-RNAPMID:19805578
affinity captured bymyo51myosin type V Affinity Capture-RNAPMID:19805578
affinity capturesmyo52myosin type V Affinity Capture-RNAPMID:19805578
binds activation domain construct withmyo52myosin type V Two-hybridPMID:18003699
affinity capturesmyo52myosin type V Affinity Capture-WesternPMID:24196839
affinity capturesmyp2myosin II heavy chain Myo3 Affinity Capture-RNAPMID:19805578
affinity captured bymyp2myosin II heavy chain Myo3 Affinity Capture-RNAPMID:19805578
affinity capturespkd2TRP-like ion channel Pkd2 Affinity Capture-WesternPMID:19543678
affinity capturesrng2IQGAP Affinity Capture-RNAPMID:19805578
affinity captured bysds23PP2A-type phosphatase inhibitor Sds23/Moc1 Affinity Capture-MSPMID:19371376
affinity capturestip1CLIP170 family protein Tip1 Affinity Capture-WesternPMID:19808886
affinity capturesypt3GTPase Ypt3 Affinity Capture-WesternPMID:22137473
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withexo70exocyst complex subunit Exo70 Synthetic LethalityPMID:21148300
phenotype enhanced byexo70exocyst complex subunit Exo70 Phenotypic EnhancementPMID:21148300
synthetic growth defect withexo70exocyst complex subunit Exo70 Synthetic Growth DefectPMID:21652630
rescuesfor3formin For3 Phenotypic SuppressionPMID:22137473
synthetic growth defect withfor3formin For3 Synthetic Growth DefectPMID:21652630
rescueskin1microtubule affinity-regulating kinase Kin1 Phenotypic SuppressionPMID:23294323
phenotype enhanced bymal3EB1 family Mal3 Phenotypic EnhancementPMID:23051734
synthetic lethal withmug33Tea1-interacting protein involved in exocytosis Synthetic LethalityPMID:21652630
enhances phenotype ofmyo51myosin type V Phenotypic EnhancementPMID:23051734
synthetic lethal withrho3Rho family GTPase Rho3 Synthetic LethalityPMID:21652630
synthetic lethal withsec8exocyst complex subunit Sec8 Synthetic LethalityPMID:21148300
phenotype enhanced bysec8exocyst complex subunit Sec8 Phenotypic EnhancementPMID:21148300
synthetic lethal withsec8exocyst complex subunit Sec8 Synthetic LethalityPMID:21652630
synthetic growth defect withtea4tip elongation aberrant protein Tea4 Synthetic Growth DefectPMID:22137473
External References
Database Identifier Description
NBRP SPCC1919.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1919.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1919.10c BioGRID Interaction Datasets
Expression Viewer SPCC1919.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1919.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1919.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1919.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1919.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1919.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC1919.10c Cell Cycle Data
GEO SPCC1919.10c GEO profiles
PInt SPCC1919.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC1919.10c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC1919.10c Fission yeast phenotypic data & analysis
SPD / RIKEN31/31A01Orfeome Localization Data
UniProtKB/SwissProtO94477Myosin-52
ModBaseO94477Database of comparative protein structure models
STRINGO94477Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588492myosin type V
RefSeq mRNANM_001023482972h- myosin type V (myo52), mRNA
European Nucleotide ArchiveD89250ENA EMBL mapping
European Nucleotide ArchiveBAA87305.1ENA Protein Mapping
European Nucleotide ArchiveCAA22641.1ENA Protein Mapping
UniParcUPI0000069836UniProt Archive

Literature for myo52

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015