myo52 (SPCC1919.10c)


Gene Standard Namemyo52 Characterisation Statuspublished
Systematic IDSPCC1919.10c Feature Typeprotein coding
Synonyms Name Description
Productmyosin type V Product Size1516aa, 175.16 kDa
Genomic Location Chromosome III, 2229932-2224882 (5051nt); CDS:2229731-2225048 (4684nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037558
GO:0003779actin bindingIEAUniProtKB-KW:KW-0009GO_REF:000003738
GO:0005516calmodulin bindingIEAUniProtKB-KW:KW-0112GO_REF:000003710
GO:0000146microfilament motor activityISMPFAM:PF00063GO_REF:00000014
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0061572actin filament bundle organizationIMPPMID:2305173414
GO:0061573actin filament bundle retrograde transportIMPPMID:230517341
GO:0034613cellular protein localization541
localizes chs2IMPPMID:16772338
GO:0071963establishment or maintenance of cell polarity regulating cell shapeIMPPMID:1111269137
GO:0070649formin-nucleated actin cable assemblyIMPPMID:230517342
GO:0000281mitotic cytokinesisIMPPMID:1111269182
GO:1990151protein localization to cell tip2
localizes for3IMPPMID:23051734
GO:0030050vesicle transport along actin filamentIMPPMID:216526301
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030479actin cortical patchIDAPMID:2305173447
GO:0051285cell cortex of cell tipIDAPMID:2414663542
IDAPMID:11112691
GO:1902716cell cortex of growing cell tip6
during monopolar cell growth, during cellular response to heatIDAPMID:24146635
GO:0032153cell division siteIDAPMID:16823372298
IDAPMID:23051734
GO:0051286cell tipIDAPMID:16823372192
IDAPMID:23051734
GO:0097575lateral cell cortex6
during cellular response to heatIDAPMID:24146635
GO:0031097medial cortex33
during mitotic cell cycleIDAPMID:11112691
GO:0016459myosin complexIEAUniProtKB-KW:KW-0518GO_REF:00000378
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assaymyo52ΔNullPECO:0000005, PECO:0000137PMID:21652630119
FYPO:0002061inviable vegetative cell populationCell growth assaymyo52ΔNullPECO:0000004, PECO:0000137PMID:216526301337
FYPO:0002060viable vegetative cell populationMicroscopymyo52ΔNullPMID:204732893751
Microscopymyo52ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002030abnormal actin cable organizationMicroscopymyo52ΔNullPMID:230517344
FYPO:0001585abolished protein localization to cell tip24
affecting exo70, sec3 and sec5Microscopymyo52ΔNullPMID:22768263
FYPO:0001324decreased protein level during vegetative growth55
affecting myo52MicroscopyΔ-tail (1162-1516)Not specifiedPMID:23051734
FYPO:0002699decreased protein localization to actomyosin contractile ring5
penetrance FYPO_EXT:0000002
affecting chs2
Microscopymyo52ΔNullPECO:0000005PMID:16772338
penetrance FYPO_EXT:0000002
affecting chs2
Microscopymyo52ΔNullPECO:0000004PMID:16772338
FYPO:0001586decreased protein localization to cell tip16
affecting mug33Microscopymyo52ΔNullPMID:21652630
FYPO:0000538decreased protein secretionEnzyme assay datamyo52ΔNullPMID:2165263014
FYPO:0002441decreased rate of actin cable retrograde transportMicroscopymyo52ΔNullPMID:230517342
FYPO:0002021dispersed actin cortical patch localizationMicroscopymyo52ΔNullPMID:2305173421
FYPO:0002444loss of punctate cytoplasmic protein localization5
affecting myo52MicroscopyΔ-tail (1162-1516)Not specifiedPMID:23051734
FYPO:0000644normal protein localization during vegetative growth119
affecting sec3Microscopymyo52ΔNullPMID:22891673
FYPO:0002442normal protein localization to cell division site8
affecting ags1Microscopymyo52ΔNullPMID:22891259
FYPO:0001587normal protein localization to cell tip17
affecting for3Microscopymyo52ΔNullPMID:23051734
affecting myo52MicroscopyΔ-tail (1162-1516)Not specifiedPMID:23051734
affecting ags1Microscopymyo52ΔNullPMID:22891259
affecting exo70, sec3 and sec5MicroscopyTea2N-CFP-Myo52C (fusion of Tea2 N-terminal motor domain, CFP, and Myo52 C-terminal domain)Not specifiedPMID:22768263
FYPO:0001120pear-shaped vegetative cellMicroscopymyo52ΔNullPMID:2305173412
FYPO:0002438short, misoriented actin cablesMicroscopymyo52ΔNullPMID:230517342
FYPO:0002380viable spheroid vegetative cellMicroscopymyo52ΔNullPECO:0000005, PECO:0000137PMID:2369780640
FYPO:0002106viable stubby vegetative cellMicroscopyΔ-tail (1162-1516)Not specifiedPMID:2305173453
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in for3 formin For3 PMID:23051734
FYPO affected by mutation in mas5 DNAJ domain protein Mas5 (predicted) PMID:24146635
FYPO affected by mutation in myo51 myosin type V PMID:23051734
FYPO affected by mutation in myo52 myosin type V PMID:23051734
FYPO affected by mutation in rng8 contractile ring myosin V regulator Rng8 PMID:24798735
GO localized by orb6 serine/threonine protein kinase Orb6 PMID:19646873
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
122299322229703
222296432229535
322294602224882
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 866 886 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 842 858 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 892 908 3
PF01843 Pfam IPR018444 Dil domain 1313 1413 3
PF00063 Pfam IPR001609 Myosin head, motor domain 76 754 5
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 864 886 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 839 861 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 887 909 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 816 838 3
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 791 813 3
SM00242 SMART IPR001609 Myosin head, motor domain 67 767 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 888 917 5
PS51126 Prosite Profiles IPR002710 Dilute 1163 1431 3
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 840 869 5
PTHR13140:SF191 HMMPANTHER 10 1092 2
PTHR13140 HMMPANTHER 10 1092 8
1.20.5.190 Gene3D 839 876 2
1.20.5.190 Gene3D 797 836 2
3.40.50.300 Gene3D 156 186 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 12 811 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 819 914 294
Coil ncoils Rabaptin coiled-coil domain 932 974 968
Coil ncoils Rabaptin coiled-coil domain 990 1025 968
PR00193 PRINTS IPR001609 Myosin head, motor domain 452 480 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 160 185 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 216 243 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 103 122 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 505 533 5

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001026IQ domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001026

Protein Properties

Ave. residue weight 115.54 Da
Charge 2.50
Isoelectric point 6.64
Molecular weight 175.16 kDa
Number of residues 1516
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS1072PMID:247631071670
S399PMID:24763107
present during mitotic M phaseS1065PMID:21712547
present during mitotic M phaseS985PMID:21712547
present during mitotic M phaseS1069PMID:21712547
present during mitotic M phaseS1072PMID:21712547
MOD:00696phosphorylated residueNASPMID:195477441922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3071during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2504during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2708during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2962during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3082during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1506.56during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1428.57during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.7during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.55during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
mal3EB1 family Mal3 Phenotypic EnhancementPMID:23051734
kin1microtubule affinity-regulating kinase Kin1 Phenotypic SuppressionPMID:23294323
exo70exocyst complex subunit Exo70 Synthetic LethalityPMID:21148300
Phenotypic Enhancement
Synthetic Growth DefectPMID:21652630
mug33Tea1-interacting protein involved in exocytosis Synthetic LethalityPMID:21652630
for3formin For3 Phenotypic SuppressionPMID:22137473
Synthetic Growth DefectPMID:21652630
myo51myosin type V Phenotypic EnhancementPMID:23051734
sec8exocyst complex subunit Sec8 Synthetic LethalityPMID:21148300
Phenotypic Enhancement
Synthetic LethalityPMID:21652630
rho3Rho family GTPase Rho3 Synthetic LethalityPMID:21652630
tea4tip elongation aberrant protein Tea4 Synthetic Growth DefectPMID:22137473
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
myo2myosin II heavy chain Affinity Capture-RNAPMID:19805578
rng2IQGAP Affinity Capture-RNAPMID:19805578
myp2myosin II heavy chain Myo3 Affinity Capture-RNAPMID:19805578
myo1myosin type I Affinity Capture-RNAPMID:19805578
cam1calmodulin Cam1 Two-hybridPMID:18003699
for3formin For3 Affinity Capture-WesternPMID:23051734
myo51myosin type V Affinity Capture-RNAPMID:19805578
tip1CLIP170 family protein Tip1 Affinity Capture-WesternPMID:19808886
dph1UBA domain protein Dph1 Affinity Capture-WesternPMID:19808886
Two-hybrid
sds23PP2A-type phosphatase inhibitor Sds23/Moc1 Affinity Capture-MSPMID:19371376
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
ypt3GTPase Ypt3 Affinity Capture-WesternPMID:22137473
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
act1actin Act1 Reconstituted ComplexPMID:24196839
pkd2TRP-like ion channel Pkd2 Affinity Capture-WesternPMID:19543678
myo52myosin type V Affinity Capture-RNAPMID:19805578
Two-hybridPMID:18003699
Affinity Capture-WesternPMID:24196839
External References
Database Identifier Description
NBRP SPCC1919.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1919.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1919.10c BioGRID Interaction Datasets
Expression Viewer SPCC1919.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1919.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1919.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1919.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1919.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1919.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC1919.10c Cell Cycle Data
GEO SPCC1919.10c GEO profiles
PInt SPCC1919.10c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC1919.10c Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN31/31A01Orfeome Localization Data
UniProtKB/SwissProtO94477Myosin-52
ModBaseO94477Database of comparative protein structure models
STRINGO94477Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588492myosin type V
RefSeq mRNANM_001023482972h- myosin type V (myo52), mRNA
European Nucleotide ArchiveD89250ENA EMBL mapping
European Nucleotide ArchiveBAA87305.1ENA Protein Mapping
European Nucleotide ArchiveCAA22641.1ENA Protein Mapping
UniParcUPI0000069836UniProt Archive

Literature for myo52

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014