myo52 (SPCC1919.10c)

Gene Standard Namemyo52 Characterisation Statuspublished
Systematic IDSPCC1919.10c Feature Typeprotein coding
Synonyms Name Description
Productmyosin type V Product Size1516aa, 175.16 kDa
Genomic Location Chromosome III, 2229932-2224882 (5051nt); CDS:2229731-2225048 (4684nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037559
GO:0003779actin bindingIEAUniProtKB-KW:KW-0009GO_REF:000003738
GO:0005516calmodulin bindingIEAUniProtKB-KW:KW-0112GO_REF:000003710
GO:0000146microfilament motor activityISMPFAM:PF00063GO_REF:00000014
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0061572actin filament bundle organizationIMPPMID:2305173414
GO:0061573actin filament bundle retrograde transportIMPPMID:230517341
GO:0034613cellular protein localization556
localizes chs2IMPPMID:16772338
GO:0071963establishment or maintenance of cell polarity regulating cell shapeIMPPMID:1111269138
GO:0000281mitotic cytokinesisIMPPMID:1111269133
GO:0090338positive regulation of formin-nucleated actin cable assemblyIMPPMID:230517342
GO:1990151protein localization to cell tip1
localizes for3IMPPMID:23051734
GO:0030050vesicle transport along actin filamentIMPPMID:216526301
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030479actin cortical patchIDAPMID:2305173447
GO:0000935barrier septumIDAPMID:2305173418
GO:0051285cell cortex of cell tipIDAPMID:1111269138
GO:0032153cell division siteIDAPMID:16823372292
GO:0051286cell tipIDAPMID:23051734186
GO:0031097medial cortex32
during mitotic cell cycleIDAPMID:11112691
GO:0016459myosin complexIEAUniProtKB-KW:KW-0518GO_REF:00000378
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assaymyo52ΔNullPECO:0000005, PECO:0000137PMID:2165263085
FYPO:0002074inviable cell population at high temperatureCell growth assaymyo52ΔNullPECO:0000137PMID:2165263017
FYPO:0002060viable vegetative cell populationMicroscopymyo52ΔNullPMID:204732893755
Microscopymyo52ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002030abnormal actin cable organizationMicroscopymyo52ΔNullPMID:230517344
FYPO:0001585abolished protein localization to cell tipMicroscopymyo52ΔNullPMID:227682636
affecting exo70
affecting sec3
affecting SPCC622.10c
FYPO:0001324decreased protein level during vegetative growthMicroscopyΔ-tail (1162-1516)Not specifiedPMID:2305173433
affecting myo52
FYPO:0001586decreased protein localization to cell tipMicroscopymyo52ΔNullPMID:216526309
affecting mug33
FYPO:0000538decreased protein secretionEnzyme assay datamyo52ΔNullPMID:216526308
FYPO:0002441decreased rate of actin cable retrograde transportMicroscopymyo52ΔNullPMID:230517342
FYPO:0002021dispersed actin cortical patch localizationMicroscopymyo52ΔNullPMID:2305173415
FYPO:0002444loss of punctate cytoplasmic localizationMicroscopyΔ-tail (1162-1516)Not specifiedPMID:230517344
affecting myo52
FYPO:0000644normal protein localization during vegetative growthMicroscopymyo52ΔNullPMID:2289167378
affecting sec3
FYPO:0001587normal protein localization to cell tipMicroscopyTea2N-CFP-Myo52C (fusion of Tea2 N-terminal motor domain,CFP,and Myo52 C-terminal domain)Not specifiedPMID:227682637
affecting exo70
affecting sec3
affecting SPCC622.10c
affecting for3Microscopymyo52ΔNullPMID:23051734
affecting myo52MicroscopyΔ-tail (1162-1516)Not specifiedPMID:23051734
FYPO:0001120pear-shaped vegetative cellMicroscopymyo52ΔNullPMID:230517344
FYPO:0002438short, misoriented actin cablesMicroscopymyo52ΔNullPMID:230517342
FYPO:0002380viable spheroid vegetative cellMicroscopymyo52ΔNullPECO:0000005, PECO:0000137PMID:2369780633
FYPO:0002106viable stubby vegetative cellMicroscopyΔ-tail (1162-1516)Not specifiedPMID:2305173449
Target Of
localized by orb6 serine/threonine protein kinase Orb6 PMID:19646873
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Start End Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01843 Pfam IPR018444 Dil domain 1313 1413 3
PF00063 Pfam IPR001609 Myosin head, motor domain 76 754 5
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 842 858 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 866 886 3
PF00612 Pfam IPR000048 IQ motif, EF-hand binding site 892 908 3
SM00242 SMART IPR001609 Myosin head, motor domain 67 767 5
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 816 838 5
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 864 886 5
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 839 861 5
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 887 909 5
SM00015 SMART IPR000048 IQ motif, EF-hand binding site 791 813 5
PS51126 Prosite Profiles IPR002710 Dilute 1163 1431 3
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 840 869 5
PS50096 Prosite Profiles IPR000048 IQ motif, EF-hand binding site 888 917 5
PTHR13140 HMMPANTHER 10 1092 8
PTHR13140:SF191 HMMPANTHER 10 1092 2
G3DSA: Gene3D 839 876 2
G3DSA: Gene3D 797 836 2
SSF52540 SuperFamily IPR027417 12 811 294
SSF52540 SuperFamily IPR027417 819 914 294
Coil ncoils Rabaptin coiled-coil domain 990 1025 975
Coil ncoils Rabaptin coiled-coil domain 932 974 975
Low complexity (SEG) seg 989 998
Low complexity (SEG) seg 1114 1124
PR00193 PRINTS IPR001609 Myosin head, motor domain 216 243 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 452 480 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 103 122 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 505 533 5
PR00193 PRINTS IPR001609 Myosin head, motor domain 160 185 5

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001026IQ domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001026

Protein Properties

Ave. residue weight 115.54 Da
Charge 2.50
Isoelectric point 6.64
Molecular weight 175.16 kDa
Number of residues 1516

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residuePMID:195477441192
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1428.57during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
1506.56during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1.7during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
0.55during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
mal3EB1 family Mal3 Phenotypic EnhancementPMID:23051734
kin1microtubule affinity-regulating kinase Kin1 Phenotypic SuppressionPMID:23294323
exo70exocyst complex subunit Exo70 Synthetic LethalityPMID:21148300
Phenotypic Enhancement
Synthetic Growth DefectPMID:21652630
mug33Tea1-interacting protein involved in exocytosis Synthetic LethalityPMID:21652630
for3formin For3 Phenotypic SuppressionPMID:22137473
Synthetic LethalityPMID:21652630
Synthetic Growth Defect
myo51myosin type V Phenotypic EnhancementPMID:23051734
sec8exocyst complex subunit Sec8 Phenotypic EnhancementPMID:21148300
Synthetic LethalityPMID:21652630
tea4tip elongation aberrant protein Tea4 Synthetic Growth DefectPMID:22137473
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
myo2myosin II heavy chain Affinity Capture-RNAPMID:19805578
myp2myosin II heavy chain Myo3 Affinity Capture-RNAPMID:19805578
myo1myosin type I Affinity Capture-RNAPMID:19805578
cam1calmodulin Cam1 Two-hybridPMID:18003699
for3formin For3 Affinity Capture-WesternPMID:23051734
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
dph1UBA domain protein Dph1 Two-hybridPMID:19808886
Affinity Capture-Western
sds23PP2A-type phosphatase inhibitor Sds23/Moc1 Affinity Capture-MSPMID:19371376
ypt3GTPase Ypt3 Affinity Capture-WesternPMID:22137473
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
pkd2TRP-like ion channel Pkd2 Affinity Capture-WesternPMID:19543678
External References
Database Identifier Description
NBRP SPCC1919.10c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC1919.10c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC1919.10c BioGRID Interaction Datasets
Expression Viewer SPCC1919.10c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC1919.10c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC1919.10c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC1919.10c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC1919.10c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC1919.10c Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC1919.10c Cell Cycle Data
GEO SPCC1919.10c GEO profiles
PInt SPCC1919.10c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2538846myosin type V
EntrezGene2538846myosin type V
SPD / RIKEN31/31A01Orfeome Localization Data
ModBaseO94477Database of comparative protein structure models
StringO94477Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588492myosin type V
RefSeq mRNANM_001023482972h- myosin type V (myo52), mRNA
European Nucleotide ArchiveBAA13911ENA Protein Mapping
European Nucleotide ArchiveBAA87305ENA Protein Mapping
European Nucleotide ArchiveCAA22641ENA Protein Mapping
UniParcUPI0000069836UniProt Archive

Literature for myo52

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014