skb5 (SPCC24B10.13)


Gene Standard Nameskb5 Characterisation Statuspublished
Systematic IDSPCC24B10.13 Feature Typeprotein coding
Synonyms Name Description
ProductShk1 kinase binding protein 5 Product Size140aa, 15.86 kDa
Genomic Location Chromosome III, 923069-923491 (423nt); CDS:923069-923491 (423nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein kinase binding2
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum596
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
slow vegetative cell population growthskb5ΔNull328
viable vegetative cell populationskb5ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologyskb5ΔNull3094
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons923069..923491
mRNA923069..923491
CDS923069..923491
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF14604 Pfam IPR001452 SH3 domain 87 137 4
SM00326 SMART IPR001452 SH3 domain 83 140 21
PS50002 Prosite Profiles IPR001452 SH3 domain 85 140 21
PTHR12602 HMMPANTHER 65 138 4
2.30.30.40 Gene3D 85 137 21
SSF50044 SuperFamily IPR001452 SH3 domain 69 138 21
PR00452 PRINTS IPR001452 SH3 domain 127 139 12
PR00452 PRINTS IPR001452 SH3 domain 83 93 12
PR00452 PRINTS IPR001452 SH3 domain 97 112 12

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.26 Da
Charge -30.50
Isoelectric point 3.63
Molecular weight 15.86 kDa
Number of residues 140
Sequence
Gene Expression

Quantitative Gene Expression

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.6during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.6during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesmkh1MEK kinase (MEKK) Mkh1 Affinity Capture-LuminescencePMID:22570491
binds DNA-binding domain construct withptc1protein phosphatase 2C Ptc1 Two-hybridPMID:23615447
binds activation domain construct withptc1protein phosphatase 2C Ptc1 Two-hybridPMID:23695164
binds DNA-binding domain construct withshk1PAK-related kinase Shk1 Two-hybridPMID:10593886
affinity captured byshk1PAK-related kinase Shk1 Affinity Capture-WesternPMID:10593886
binds DNA-binding domain construct withshk1PAK-related kinase Shk1 Two-hybridPMID:23615447
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withcdt2WD repeat protein Cdt2 Positive GeneticPMID:22681890
negative genetic interaction withclr1SHREC complex subunit Clr1 Negative GeneticPMID:22681890
negative genetic interaction withcwf28splicing factor Cwf28 Negative GeneticPMID:22681890
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:22681890
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
negative genetic interaction withdsc4Golgi Dsc E3 ligase complex subunit Dsc4 Negative GeneticPMID:21504829
negative genetic interaction withdsc4Golgi Dsc E3 ligase complex subunit Dsc4 Negative GeneticPMID:22681890
negative genetic interaction witheif21translation initiation factor eIF2A (predicted) Negative GeneticPMID:22681890
negative genetic interaction withglc8protein phosphatase regulatory subunit Glc8 (predicted) Negative GeneticPMID:22681890
synthetic growth defect withgmh5alpha-1,2-galactosyltransferase (predicted) Synthetic Growth DefectPMID:18931302
negative genetic interaction withgmh5alpha-1,2-galactosyltransferase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
negative genetic interaction withomh6alpha-1,2-mannosyltransferase Omh6 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpck1protein kinase C (PKC)-like Pck1 Negative GeneticPMID:22681890
negative genetic interaction withpmp1dual-specificity MAP kinase phosphatase Pmp1 Negative GeneticPMID:22681890
negative genetic interaction withptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
negative genetic interaction withpub1HECT-type ubiquitin-protein ligase E3 Pub1 Negative GeneticPMID:22681890
synthetic growth defect withpyp1tyrosine phosphatase Pyp1 Synthetic Growth DefectPMID:18931302
positive genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withsan1sir antagonist, ubiquitin-protein ligase E3 Positive GeneticPMID:22681890
negative genetic interaction withsgf73SAGA complex subunit Sgf73 Negative GeneticPMID:25076038
synthetically rescuesshk1PAK-related kinase Shk1 Synthetic RescuePMID:10593886
rescued by overexpression ofshk1PAK-related kinase Shk1 Dosage RescuePMID:10593886
rescued by overexpression ofskb1type II protein arginine N-methyltransferase Skb1 Dosage RescuePMID:10593886
negative genetic interaction withsty1MAP kinase Sty1 Negative GeneticPMID:22681890
positive genetic interaction withtom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withubp4ubiquitin C-terminal hydrolase Ubp4 Positive GeneticPMID:22681890
negative genetic interaction withuri1unconventional prefoldin chaperone involved protein complex assembly Uri1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withvps17retromer complex subunit Vps17 Negative GeneticPMID:22681890
negative genetic interaction withvps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
negative genetic interaction withypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
negative genetic interaction withypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPCC24B10.13 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC24B10.13 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC24B10.13 BioGRID Interaction Datasets
Expression Viewer SPCC24B10.13 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC24B10.13 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC24B10.13 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC24B10.13 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC24B10.13 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC24B10.13 Transcriptome Viewer (Bähler Lab)
GEO SPCC24B10.13 GEO profiles
PInt SPCC24B10.13 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC24B10.13 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC24B10.13 Fission yeast phenotypic data & analysis
Cyclebase SPCC24B10.13.1 Cell Cycle Data
SPD / RIKEN03/03D12Orfeome Localization Data
UniProtKB/SwissProtQ9US59Shk1 kinase-binding protein 5
ModBaseQ9US59Database of comparative protein structure models
STRINGQ9US59Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588016Shk1 kinase binding protein 5
RefSeq mRNANM_001023007972h- Shk1 kinase binding protein 5 (skb5), mRNA
European Nucleotide ArchiveAAF19175.1ENA Protein Mapping
European Nucleotide ArchiveCAB76222.1ENA Protein Mapping
UniParcUPI000006A970UniProt Archive

Literature for skb5

Search: Europe PMC or PubMed

Release Version: PomBase:26_52 - 11 May 2015