skb5 (SPCC24B10.13)


Gene Standard Nameskb5 Characterisation Statuspublished
Systematic IDSPCC24B10.13 Feature Typeprotein coding
Synonyms Name Description
ProductShk1 kinase binding protein 5 Product Size140aa, 15.86 kDa
Genomic Location Chromosome III, 923069-923491 (423nt); CDS:923069-923491 (423nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0019901protein kinase bindingIPIshk1PMID:105938862
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005783endoplasmic reticulumIDAPMID:16823372594
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopyskb5ΔNullPMID:204732893760
Microscopyskb5ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3086
penetrance FYPO_EXT:0000001Microscopyskb5ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1923069923491
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF14604 Pfam IPR001452 SH3 domain 87 137 3
SM00326 SMART IPR001452 SH3 domain 83 140 21
PS50002 Prosite Profiles IPR001452 SH3 domain 85 140 21
PTHR12602 HMMPANTHER 65 138 4
2.30.30.40 Gene3D 85 137 21
SSF50044 SuperFamily IPR001452 SH3 domain 69 138 21
PR00452 PRINTS IPR001452 SH3 domain 83 93 12
PR00452 PRINTS IPR001452 SH3 domain 97 112 12
PR00452 PRINTS IPR001452 SH3 domain 127 139 12

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.26 Da
Charge -30.50
Isoelectric point 3.63
Molecular weight 15.86 kDa
Number of residues 140
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
NDduring GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
4.6during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
1.6during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
vps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
SPCC736.07cunconventional prefoldin involved in translation initiation (predicted) Negative GeneticPMID:22681890
cdt2WD repeat protein Cdt2 Positive GeneticPMID:22681890
vps17retromer complex subunit Vps17 Negative GeneticPMID:22681890
cwf28splicing factor Cwf28 Negative GeneticPMID:22681890
clr1cryptic loci regulator Clr1 Negative GeneticPMID:22681890
pyp1tyrosine phosphatase Pyp1 Synthetic Growth DefectPMID:18931302
glc9protein phosphatase regulatory subunit Glc9 (predicted) Negative GeneticPMID:22681890
gmh5alpha-1,2-galactosyltransferase (predicted) Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:22681890
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:22681890
sty1MAP kinase Sty1 Negative GeneticPMID:22681890
pck1protein kinase C (PKC)-like Pck1 Negative GeneticPMID:22681890
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
ubp4ubiquitin C-terminal hydrolase Ubp4 Positive GeneticPMID:22681890
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
skb1type II protein arginine N-methyltransferase Skb1 Dosage RescuePMID:10593886
pmp1dual-specificity MAP kinase phosphatase Pmp1 Negative GeneticPMID:22681890
tom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
ypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
rpl160160S ribosomal protein L13/L16 (predicted) Positive GeneticPMID:22681890
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
omh6alpha-1,2-mannosyltransferase Omh6 (predicted) Negative GeneticPMID:22681890
kin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
san1sir antagonist, ubiquitin-protein ligase E3 Positive GeneticPMID:22681890
SPBC4B4.04translation initiation factor eIF2A (predicted) Negative GeneticPMID:22681890
pub1HECT-type ubiquitin-protein ligase E3 Pub1 Negative GeneticPMID:22681890
shk1PAK-related kinase Shk1 Synthetic RescuePMID:10593886
Dosage Rescue
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
ptc1protein phosphatase 2C Ptc1 Two-hybridPMID:23615447
Two-hybridPMID:23695164
mkh1MEK kinase (MEKK) Mkh1 Affinity Capture-LuminescencePMID:22570491
shk1PAK-related kinase Shk1 Affinity Capture-WesternPMID:10593886
Two-hybrid
Two-hybridPMID:23615447
External References
Database Identifier Description
NBRP SPCC24B10.13 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC24B10.13 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC24B10.13 BioGRID Interaction Datasets
Expression Viewer SPCC24B10.13 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC24B10.13 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC24B10.13 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC24B10.13 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC24B10.13 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC24B10.13 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC24B10.13 Cell Cycle Data
GEO SPCC24B10.13 GEO profiles
PInt SPCC24B10.13 Protein-Protein Interaction Predictor (Bähler Lab)
SPD / RIKEN03/03D12Orfeome Localization Data
WikiGene2539237Shk1 kinase binding protein 5
EntrezGene2539237Shk1 kinase binding protein 5
UniProtKB/SwissProtQ9US59Shk1 kinase-binding protein 5
ModBaseQ9US59Database of comparative protein structure models
STRINGQ9US59Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588016Shk1 kinase binding protein 5
RefSeq mRNANM_001023007972h- Shk1 kinase binding protein 5 (skb5), mRNA
European Nucleotide ArchiveAAF19175ENA Protein Mapping
European Nucleotide ArchiveAAF19175.1ENA Protein Mapping
European Nucleotide ArchiveCAB76222ENA Protein Mapping
European Nucleotide ArchiveCAB76222.1ENA Protein Mapping
UniParcUPI000006A970UniProt Archive

Literature for skb5

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014