cgs2 (SPCC285.09c)


Gene Standard Namecgs2 Characterisation Statuspublished
Systematic IDSPCC285.09c Feature Typeprotein coding
Synonymspde1 Name DescriptionContinues to Grow in Stationary
ProductcAMP-specific phosphodiesterase Cgs2 Product Size346aa, 38.65 kDa
Genomic Location Chromosome III, 1812646-1810337 (2310nt); CDS:1812631-1811032 (1600nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
3',5'-cyclic-AMP phosphodiesterase activity1
Annotation ExtensionEvidenceWith/FromReference
3',5'-cyclic-GMP phosphodiesterase activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cAMP catabolic process1
Annotation ExtensionEvidenceWith/FromReference
cellular response to glucose stimulus8
Annotation ExtensionEvidenceWith/FromReference
cellular response to nitrogen starvation21
Annotation ExtensionEvidenceWith/FromReference
negative regulation of cAMP biosynthetic process2
Annotation ExtensionEvidenceWith/FromReference
negative regulation of cAMP-dependent protein kinase activity1
Annotation ExtensionEvidenceWith/FromReference
negative regulation of glucose mediated signaling pathway3
Annotation ExtensionEvidenceWith/FromReference
negative regulation of meiotic cell cycle16
Annotation ExtensionEvidenceWith/FromReference
regulation of cAMP-mediated signaling8
Annotation ExtensionEvidenceWith/FromReference
regulation of signal transduction involved in conjugation with cellular fusion11
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased mating efficiencycgs2Δ272
decreased vegetative cell population growthcgs2+657
increased mating efficiencycgs2+36
loss of viability in stationary phasecgs2Δ61
cgs2-1
loss of viability upon nitrogen starvationcgs2Δ53
sensitive to bortezomibcgs2Δ255
sensitive to camptothecincgs2Δ248
viable vegetative cell populationcgs2Δ3840

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic cell cyclecgs2Δ877
decreased cellular cAMP levelcgs2+4
elongated cell during stationary phasecgs2-14
increased cellular cAMP levelcgs2Δ3
cgs2-1
increased cellular cAMP level during cellular response to glucose stimuluscgs2Δ2
normal protein serine phosphorylation during vegetative growth12
affecting gad8cgs2Δ
viable elongated vegetative cellcgs2Δ318
viable vegetative cell with normal cell morphologycgs2Δ3100

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
increased mating efficiencycyr1Δ, cgs2-2 (frameshifted allele)
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in prp2 U2AF large subunit (U2AF-59)
GO regulated by pcr1 transcription factor Pcr1
GO regulated by sty1 MAP kinase Sty1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1812646..1812542, 1812480..1812472, 1812425..1812013, 1811560..1810337
Intron1812541..1812481, 1812471..1812426, 1812012..1811561
mRNA1812646..1810337
5' UTR1812646..1812632PMID:18641648
5' UTR1812646..1812632PMID:20118936
CDS1812631..1812542, 1812480..1812472, 1812425..1812013, 1811560..1811032
3' UTR1811031..1810337PMID:18641648
3' UTR1811031..1810337PMID:20118936
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02112 Pfam IPR000396 Cyclic-AMP phosphodiesterase, class-II 25 341 1
PS00607 Prosite Patterns IPR024225 Cyclic-AMP phosphodiesterase class-II, conserved site 114 128 1
PTHR28283:SF1 HMMPANTHER 1 346 1
PTHR28283 HMMPANTHER 1 346 1
3.60.15.10 Gene3D IPR001279 Metallo-beta-lactamase 42 111 9
3.60.15.10 Gene3D IPR001279 Metallo-beta-lactamase 112 225 9
SSF56281 SuperFamily IPR001279 Metallo-beta-lactamase 103 280 9
PIRSF000962 PIRSF IPR000396 Cyclic-AMP phosphodiesterase, class-II 1 346 1
PR00388 PRINTS IPR000396 Cyclic-AMP phosphodiesterase, class-II 249 260 1
PR00388 PRINTS IPR000396 Cyclic-AMP phosphodiesterase, class-II 110 128 1
PR00388 PRINTS IPR000396 Cyclic-AMP phosphodiesterase, class-II 140 161 1
PR00388 PRINTS IPR000396 Cyclic-AMP phosphodiesterase, class-II 267 280 1
PR00388 PRINTS IPR000396 Cyclic-AMP phosphodiesterase, class-II 294 305 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.71 Da
Charge -2.00
Codon Adaptation Index 0.41
Isoelectric point 6.29
Molecular weight 38.65 kDa
Number of residues 346
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for cgs2 (SPCC285.09c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3974during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5429during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3332during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4082during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4115during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4490.79during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2111.99during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.61during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Pathway
DescriptionQualifierReferenceCount
links stress-activated MAPK (Sty1) pathway to cAMP-dependent protein kinase (Pka1) pathway PMID:154481373
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4606
conserved in bacteria1001
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPCC285.09c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withatf1transcription factor, Atf-CREB family Atf1 Synthetic LethalityPMID:8557039
rescuespat1serine/threonine protein kinase Ran1/Pat1 Phenotypic SuppressionPMID:1657594
rescuescyr1adenylate cyclase Phenotypic SuppressionPMID:21118717
rescued bypac2cAMP-independent regulatory protein Pac2 Phenotypic SuppressionPMID:8536311
rescued bysak1transcriptional repressor Sak1 Phenotypic SuppressionPMID:7862141
enhances phenotype ofgpa2heterotrimeric G protein alpha-2 subunit Gpa2 Phenotypic EnhancementPMID:1340462
positive genetic interaction withtps1alpha,alpha-trehalose-phosphate synthase [UDP-forming] Positive GeneticPMID:22681890
positive genetic interaction withelp3elongator complex subunit Elp3 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withiwr1RNA polymerase II nuclear import protein Iwr1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withpar2protein phosphatase PP2A regulatory subunit B-56 Par2 Positive GeneticPMID:22681890
positive genetic interaction withmpr1histidine-containing response regulator phosphotransferase Mpr1 Positive GeneticPMID:22681890
positive genetic interaction withhip1hira protein, histone chaperone Hip1 Positive GeneticPMID:22681890
positive genetic interaction withask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
positive genetic interaction withhus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
positive genetic interaction withhip4histone promoter control protein Hip4 Positive GeneticPMID:22681890
positive genetic interaction withsty1MAP kinase Sty1 Positive GeneticPMID:22681890
positive genetic interaction withSPBC582.10cATP-dependent DNA helicase Rhp16b (predicted) Positive GeneticPMID:22681890
positive genetic interaction withelp2elongator complex subunit Elp2 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
negative genetic interaction withmph1dual specificity protein kinase Mph1 Negative GeneticPMID:22681890
negative genetic interaction withSPBC17A3.06phosphoprotein phosphatase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withngg1SAGA complex subunit Ngg1 Negative GeneticPMID:22681890
negative genetic interaction withvph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withswd3WD repeat protein Swd3 Negative GeneticPMID:22681890
negative genetic interaction withrec14Ski complex subunit Rec14 Negative GeneticPMID:22681890
negative genetic interaction witharp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
negative genetic interaction withnot3CCR4-Not complex subunit Not3/5 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withubp2ubiquitin C-terminal hydrolase Ubp2 Negative GeneticPMID:22681890
negative genetic interaction withmob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Negative GeneticPMID:22681890
negative genetic interaction withglc8protein phosphatase regulatory subunit Glc8 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC1685.04Schizosaccharomyces specific protein Negative GeneticPMID:22681890
negative genetic interaction withrxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
negative genetic interaction withsum2translation initiation inhibitor (predicted) Negative GeneticPMID:22681890
negative genetic interaction withypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:22681890
negative genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
negative genetic interaction withcdc13G2/M B-type cyclin Cdc13 Negative GeneticPMID:22681890
negative genetic interaction withSPBC839.03cneddylation protein Dcn1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withtif38translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
negative genetic interaction withrnc1RNA-binding protein that suppresses calcineurin deletion Rnc1 Negative GeneticPMID:22681890
negative genetic interaction withsts5RNB-like protein Negative GeneticPMID:22681890
synthetically rescuesgpa2heterotrimeric G protein alpha-2 subunit Gpa2 Synthetic RescuePMID:16143612
synthetically rescuesgit3G-protein coupled receptor Git3 Synthetic RescuePMID:16143612
synthetically rescuescyr1adenylate cyclase Synthetic RescuePMID:16143612
overexpression rescuescut9anaphase-promoting complex, TPR lobe subcomplex subunit Cut9/Apc6 Dosage RescuePMID:15507118
overexpression rescuescut20anaphase-promoting complex, platform subcomplex scaffold subunit Apc4/Cut20 Dosage RescuePMID:10526233
overexpression rescuescut23anaphase-promoting complex, TPR lobe subcomplex subunit Apc8 Dosage RescuePMID:15507118
overexpression rescuescut4anaphase-promoting complex, platform subcomplex scaffold subunit Apc1 Dosage RescuePMID:8918880
External References
Database Identifier Description
NBRP SPCC285.09c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC285.09c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC285.09c BioGRID Interaction Datasets
Expression Viewer SPCC285.09c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC285.09c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC285.09c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC285.09c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC285.09c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC285.09c Transcriptome Viewer (Bähler Lab)
GEO SPCC285.09c GEO profiles
PInt SPCC285.09c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC285.09c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC285.09c Fission yeast phenotypic data & analysis
Cyclebase SPCC285.09c.1 Cell Cycle Data
SPD / RIKEN25/25F07Orfeome Localization Data
IntEnz3.1.4.17Integrated relational Enzyme database
Rhea3.1.4.17Annotated reactions database
UniProtKB/SwissProtP365993',5'-cyclic-nucleotide phosphodiesterase
ModBaseP36599Database of comparative protein structure models
STRINGP36599Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588337cAMP-specific phosphodiesterase Cgs2
RefSeq mRNANM_001023328972h- cAMP-specific phosphodiesterase Cgs2 (cgs2), mRNA

Literature for cgs2

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016