cgs2 (SPCC285.09c)


Gene Standard Namecgs2 Characterisation Statuspublished
Systematic IDSPCC285.09c Feature Typeprotein coding
Synonymspde1 Name DescriptionContinues to Grow in Stationary
ProductcAMP-specific phosphodiesterase Cgs2 Product Size346aa, 38.65 kDa
Genomic Location Chromosome III, 1812646-1810337 (2310nt); CDS:1812631-1811032 (1600nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
3',5'-cyclic-AMP phosphodiesterase activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cAMP catabolic process1
Annotation ExtensionEvidenceWith/FromReference
cellular response to glucose stimulus9
Annotation ExtensionEvidenceWith/FromReference
cellular response to nitrogen starvation82
Annotation ExtensionEvidenceWith/FromReference
negative regulation of adenylate cyclase activity1
Annotation ExtensionEvidenceWith/FromReference
negative regulation of cAMP biosynthetic process2
Annotation ExtensionEvidenceWith/FromReference
negative regulation of meiotic cell cycle12
Annotation ExtensionEvidenceWith/FromReference
positive regulation of transcription from RNA polymerase II promoter87
Annotation ExtensionEvidenceWith/FromReference
regulation of cAMP-mediated signaling8
Annotation ExtensionEvidenceWith/FromReference
regulation of signal transduction involved in conjugation with cellular fusion12
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2316
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased mating efficiencycgs2ΔNull247
decreased vegetative cell population growthcgs2+ (wild type)Overexpression400
increased mating efficiencycgs2+ (wild type)Overexpression27
inviable cell populationcgs2ΔNull1433
loss of viability in stationary phasecgs2-1Not specified52
cgs2ΔNull
sensitive to bortezomib256
expressivity FYPO_EXT:0000003cgs2ΔNull
sensitive to camptothecincgs2ΔNull214
viable vegetative cell populationcgs2ΔNull3781

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic cell cyclecgs2ΔNull794
decreased cellular cAMP levelcgs2+ (wild type)Overexpression4
elongated cell during stationary phasecgs2-1Not specified4
increased cellular cAMP levelcgs2-1Not specified3
cgs2ΔNull
increased cellular cAMP level during cellular response to glucose stimulus2
expressivity FYPO_EXT:0000002cgs2ΔNull
normal RNA level during nitrogen starvation10
affecting fnx1cgs2-2Endogenous
viable elongated vegetative cell231
penetrance FYPO_EXT:0000001cgs2ΔNull
viable vegetative cell with normal cell morphology3092
penetrance FYPO_EXT:0000001cgs2ΔNull
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in prp2 U2AF large subunit (U2AF-59) PMID:9917066
GO regulated by pcr1 transcription factor Pcr1 PMID:15448137
GO regulated by sty1 MAP kinase Sty1 PMID:15448137
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
118126461812542
218124801812472
318124251812013
418115601810337

UTRs

Region Coordinates Reference
five_prime_UTR1812646..1812632PMID:18641648
three_prime_UTR1811031..1810337PMID:18641648
five_prime_UTR1812646..1812632PMID:20118936
three_prime_UTR1811031..1810337PMID:20118936
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02112 Pfam IPR000396 Cyclic-AMP phosphodiesterase, class-II 25 341 1
PS00607 Prosite Patterns IPR024225 Cyclic-AMP phosphodiesterase class-II, conserved site 114 128 1
3.60.15.10 Gene3D IPR001279 Beta-lactamase-like 112 225 9
3.60.15.10 Gene3D IPR001279 Beta-lactamase-like 42 111 9
SSF56281 SuperFamily IPR001279 Beta-lactamase-like 103 280 9
PR00388 PRINTS IPR000396 Cyclic-AMP phosphodiesterase, class-II 140 161 1
PR00388 PRINTS IPR000396 Cyclic-AMP phosphodiesterase, class-II 267 280 1
PR00388 PRINTS IPR000396 Cyclic-AMP phosphodiesterase, class-II 249 260 1
PR00388 PRINTS IPR000396 Cyclic-AMP phosphodiesterase, class-II 294 305 1
PR00388 PRINTS IPR000396 Cyclic-AMP phosphodiesterase, class-II 110 128 1
PIRSF000962 PIRSF IPR000396 Cyclic-AMP phosphodiesterase, class-II 1 346 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.71 Da
Charge -2.00
Isoelectric point 6.29
Molecular weight 38.65 kDa
Number of residues 346
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3974during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5429during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3332during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
4082during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
4490.79during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
4115during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2111.99during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.61during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Pathway
DescriptionQualifierReferenceCount
links stress-activated MAPK (Sty1) pathway to cAMP-dependent protein kinase (Pka1) pathway PMID:154481373
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in bacteria1000
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
cut4anaphase-promoting complex subunit Apc1 Dosage RescuePMID:8918880
hip1hira protein, histone chaperone Hip1 Positive GeneticPMID:22681890
gpa2heterotrimeric G protein alpha-2 subunit Gpa2 Synthetic RescuePMID:16143612
Phenotypic Enhancement
Phenotypic EnhancementPMID:1340462
mph1dual specificity protein kinase Mph1 Negative GeneticPMID:22681890
SPBC1685.04Schizosaccharomyces specific protein Negative GeneticPMID:22681890
iwr1RNA polymerase II nuclear import protein Iwr1 (predicted) Positive GeneticPMID:22681890
swd3WD repeat protein Swd3 Negative GeneticPMID:22681890
glc8protein phosphatase regulatory subunit Glc8 (predicted) Negative GeneticPMID:22681890
rxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:22681890
SPBC839.03cneddylation protein Dcn1 (predicted) Negative GeneticPMID:22681890
elp3elongator complex subunit Elp3 (predicted) Positive GeneticPMID:22681890
rec14Ski complex subunit Rec14 Negative GeneticPMID:22681890
SPAC821.05translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
mpr1histidine-containing response regulator phosphotransferase Mpr1 Positive GeneticPMID:22681890
sty1MAP kinase Sty1 Positive GeneticPMID:22681890
par2protein phosphatase regulatory subunit Par2 Positive GeneticPMID:22681890
mob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Negative GeneticPMID:22681890
hus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
ask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
cut23anaphase-promoting complex subunit Apc8 Dosage RescuePMID:15507118
msa1RNA-binding protein Msa1 Phenotypic SuppressionPMID:15166138
cyr1adenylate cyclase Synthetic RescuePMID:16143612
SPBC582.10cATP-dependent DNA helicase Rhp16b (predicted) Positive GeneticPMID:22681890
not3CCR4-Not complex subunit Not3/5 (predicted) Negative GeneticPMID:22681890
atf1transcription factor, Atf-CREB family Atf1 Synthetic LethalityPMID:8557039
SPBC17A3.06phosphoprotein phosphatase (predicted) Negative GeneticPMID:22681890
git3G-protein coupled receptor Git3 Synthetic RescuePMID:16143612
arp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
sts5RNB-like protein Negative GeneticPMID:22681890
hip4histone promoter control protein Hip4 Positive GeneticPMID:22681890
ngg1SAGA complex subunit Ngg1 Negative GeneticPMID:22681890
cut9anaphase-promoting complex subunit Cut9 Dosage RescuePMID:15507118
pac2cAMP-independent regulatory protein Pac2 Phenotypic SuppressionPMID:8536311
sak1transcriptional repressor Sak1 Phenotypic SuppressionPMID:7862141
ran1serine/threonine protein kinase Ran1 Phenotypic SuppressionPMID:1657594
prz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
tps1alpha,alpha-trehalose-phosphate synthase Positive GeneticPMID:22681890
cdc13G2/M B-type cyclin Cdc13 Negative GeneticPMID:22681890
elp2elongator complex subunit Elp2 (predicted) Positive GeneticPMID:22681890
ubp2ubiquitin C-terminal hydrolase Ubp2 Negative GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
rnc1RNA-binding protein that suppresses calcineurin deletion Rnc1 Negative GeneticPMID:22681890
cut20anaphase-promoting complex subunit Apc4/Cut20 Dosage RescuePMID:10526233
sum2G2/M transition checkpoint protein Sum2 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPCC285.09c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC285.09c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC285.09c BioGRID Interaction Datasets
Expression Viewer SPCC285.09c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC285.09c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC285.09c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC285.09c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC285.09c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC285.09c Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC285.09c Cell Cycle Data
GEO SPCC285.09c GEO profiles
PInt SPCC285.09c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC285.09c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC285.09c Fission yeast phenotypic data & analysis
IntEnz3.1.4.17Integrated relational Enzyme database
Rhea3.1.4.17Annotated reactions database
SPD / RIKEN25/25F07Orfeome Localization Data
UniProtKB/SwissProtP365993',5'-cyclic-nucleotide phosphodiesterase
ModBaseP36599Database of comparative protein structure models
STRINGP36599Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588337cAMP-specific phosphodiesterase Cgs2
RefSeq mRNANM_001023328972h- cAMP-specific phosphodiesterase Cgs2 (cgs2), mRNA
European Nucleotide ArchiveAAB20315.1ENA Protein Mapping
European Nucleotide ArchiveCAA20848.1ENA Protein Mapping
UniParcUPI0000127C05UniProt Archive

Literature for cgs2

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015