cgs2 (SPCC285.09c)


Gene Standard Namecgs2 Characterisation Statuspublished
Systematic IDSPCC285.09c Feature Typeprotein coding
Synonymspde1 Name Description
ProductcAMP-specific phosphodiesterase Cgs2 Product Size346aa, 38.65 kDa
Genomic Location Chromosome III, 1812646-1810337 (2310nt); CDS:1812631-1811032 (1600nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:00041153',5'-cyclic-AMP phosphodiesterase activity1
heterologous_systemIGIUniProtKB:P22434PMID:8392846
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006198cAMP catabolic processIMPPMID:114618991
GO:0019933cAMP-mediated signalingIMPPMID:13184975
GO:0071333cellular response to glucose stimulusIMPPMID:13404622
GO:0006995cellular response to nitrogen starvationIMPPMID:1544813793
GO:0007194negative regulation of adenylate cyclase activityTASPMID:161436121
GO:0030818negative regulation of cAMP biosynthetic processIMPPMID:13404622
GO:0045944positive regulation of transcription from RNA polymerase II promoterIMPPMID:1544813785
GO:0031137regulation of conjugation with cellular fusionIMPPMID:131849762
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722317
GO:0005634nucleusIDAPMID:168233722730
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001701sensitive to bortezomibCell growth assaycgs2ΔNullPECO:0000005, PECO:0000137PMID:21760946256
expressivity FYPO_EXT:0000003
FYPO:0000085sensitive to camptothecincompetitive growth assay evidencecgs2ΔNullPMID:20537132200
FYPO:0002060viable vegetative cell populationMicroscopycgs2ΔNullPMID:204732893755
Microscopycgs2ΔNullPECO:0000004, PECO:0000137PMID:23697806
Microscopycgs2ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopycgs2ΔNullPECO:0000004, PECO:0000137PMID:23697806564
FYPO:0001664increased cellular cAMP level during cellular response to glucose stimulusSubstance quantification evidencecgs2ΔNullPECO:0000126PMID:13404622
expressivity FYPO_EXT:0000002
FYPO:0001096normal RNA level during nitrogen starvationWestern blot assaycgs2-2EndogenousPECO:0000103, PECO:0000126PMID:97106088
affecting fnx1
FYPO:0001492viable elongated vegetative cellMicroscopycgs2ΔNullPECO:0000004, PECO:0000137PMID:23697806170
penetrance FYPO_EXT:0000001
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopycgs2ΔNullPECO:0000005, PECO:0000137PMID:236978063078
penetrance FYPO_EXT:0000001
Target Of
RelationshipGeneProductReference
regulated by pcr1 transcription factor Pcr1 PMID:15448137
regulated by sty1 MAP kinase Sty1 PMID:15448137
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
118126461812542
218124801812472
318124251812013
418115601810337

UTRs

Region Start End Reference
five_prime_UTR18126461812632PMID:18641648
three_prime_UTR18110311810337PMID:18641648
three_prime_UTR18110311810337PMID:20118936
five_prime_UTR18126461812632PMID:20118936
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02112 Pfam IPR000396 Cyclic-AMP phosphodiesterase, class-II 25 341 1
PS00607 Prosite Patterns IPR024225 Cyclic-AMP phosphodiesterase, class-II 114 128 1
G3DSA:3.60.15.10 Gene3D IPR001279 71 279 9
SSF56281 SuperFamily 103 280 9
Low complexity (SEG) seg 319 332
PR00388 PRINTS IPR000396 Cyclic-AMP phosphodiesterase, class-II 294 305 1
PR00388 PRINTS IPR000396 Cyclic-AMP phosphodiesterase, class-II 267 280 1
PR00388 PRINTS IPR000396 Cyclic-AMP phosphodiesterase, class-II 140 161 1
PR00388 PRINTS IPR000396 Cyclic-AMP phosphodiesterase, class-II 110 128 1
PR00388 PRINTS IPR000396 Cyclic-AMP phosphodiesterase, class-II 249 260 1
PIRSF000962 PIRSF IPR000396 Cyclic-AMP phosphodiesterase, class-II 1 346 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.71 Da
Charge -2.00
Isoelectric point 6.29
Molecular weight 38.65 kDa
Number of residues 346
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
4490.79during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
2111.99during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.61during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Pathway
DescriptionQualifierReferenceCount
links stress-activated MAPK (Sty1) pathway to cAMP-dependent protein kinase (Pka1) pathway PMID:154481373
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4564
conserved in bacteria1001
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
cut4anaphase-promoting complex subunit Apc1 Dosage RescuePMID:8918880
hip1hira protein, histone chaperone Hip1 Positive GeneticPMID:22681890
gpa2heterotrimeric G protein alpha-2 subunit Gpa2 Synthetic RescuePMID:16143612
Phenotypic Enhancement
Phenotypic EnhancementPMID:1340462
mph1dual specificity protein kinase Mph1 Negative GeneticPMID:22681890
SPBC1685.04sequence orphan Negative GeneticPMID:22681890
iwr1RNA polymerase II nuclear import protein Iwr1 (predicted) Positive GeneticPMID:22681890
swd3WD repeat protein Swd3 Negative GeneticPMID:22681890
SPAC17A5.09cprotein phosphatase regulatory subunit Glc9 (predicted) Negative GeneticPMID:22681890
rxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:22681890
SPBC839.03cneddylation protein Dcn1 (predicted) Negative GeneticPMID:22681890
elp3elongator complex, histone acetyltransferase subunit Elp3 (predicted) Positive GeneticPMID:22681890
rec14Ski complex subunit Rec14 Negative GeneticPMID:22681890
SPAC821.05translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
mpr1histidine-containing response regulator phosphotransferase Mpr1 Positive GeneticPMID:22681890
sty1MAP kinase Sty1 Positive GeneticPMID:22681890
par2protein phosphatase regulatory subunit Par2 Positive GeneticPMID:22681890
git3G-protein coupled receptor Git3 Synthetic RescuePMID:16143612
hus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
ask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
cut23anaphase-promoting complex subunit Apc8 Dosage RescuePMID:15507118
msa1RNA-binding protein Msa1 Phenotypic SuppressionPMID:15166138
cyr1adenylate cyclase Synthetic RescuePMID:16143612
SPBC582.10cATP-dependent DNA helicase Rhp16b (predicted) Positive GeneticPMID:22681890
not3CCR4-Not complex subunit Not3/5 (predicted) Negative GeneticPMID:22681890
SPBC17A3.06phosphoprotein phosphatase (predicted) Negative GeneticPMID:22681890
mob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Negative GeneticPMID:22681890
arp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
sts5RNB-like protein Negative GeneticPMID:22681890
hip4histone promoter control protein Hip4 Positive GeneticPMID:22681890
ngg1SAGA complex subunit Ngg1 Negative GeneticPMID:22681890
cut9anaphase-promoting complex subunit Cut9 Dosage RescuePMID:15507118
sak1transcriptional repressor Sak1 Phenotypic SuppressionPMID:7862141
prz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
tps1alpha,alpha-trehalose-phosphate synthase Positive GeneticPMID:22681890
cdc13G2/M B-type cyclin Cdc13 Negative GeneticPMID:22681890
elp2elongator complex subunit Elp2 (predicted) Positive GeneticPMID:22681890
ubp2ubiquitin C-terminal hydrolase Ubp2 Negative GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
rnc1RNA-binding protein that suppresses calcineurin deletion Rnc1 Negative GeneticPMID:22681890
cut20anaphase-promoting complex subunit Apc4 Dosage RescuePMID:10526233
sum2G2/M transition checkpoint protein Sum2 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPCC285.09c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC285.09c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC285.09c BioGRID Interaction Datasets
Expression Viewer SPCC285.09c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC285.09c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC285.09c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC285.09c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC285.09c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC285.09c Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC285.09c Cell Cycle Data
GEO SPCC285.09c GEO profiles
PInt SPCC285.09c Protein-Protein Interaction Predictor (Bähler Lab)
IntEnz3.1.4.17Integrated relational Enzyme database
Rhea3.1.4.17Annotated reactions database
SPD / RIKEN25/25F07Orfeome Localization Data
EntrezGene2539207cAMP-specific phosphodiesterase Cgs2
WikiGene2539207cAMP-specific phosphodiesterase Cgs2
UniProtKB/SwissProtP365993',5'-cyclic-nucleotide phosphodiesterase
ModBaseP36599Database of comparative protein structure models
StringP36599Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588337cAMP-specific phosphodiesterase Cgs2
RefSeq mRNANM_001023328972h- cAMP-specific phosphodiesterase Cgs2 (cgs2), mRNA
European Nucleotide ArchiveAAB20315ENA Protein Mapping
European Nucleotide ArchiveCAA20848ENA Protein Mapping
UniParcUPI0000127C05UniProt Archive

Literature for cgs2

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014