big1 (SPCC306.06c)

Gene Standard Namebig1 Characterisation Statusbiological_role_inferred
Systematic IDSPCC306.06c Feature Typeprotein coding
Synonyms Name Description
ProductER membrane protein, BIG1 family (predicted) Product Size311aa, 34.64 kDa
Genomic Location Chromosome III, 419553-418005 (1549nt); CDS:419473-418476 (998nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
(1->6)-beta-D-glucan biosynthetic process6
Annotation ExtensionEvidenceWith/FromReference
fungal-type cell wall biogenesis70
Annotation ExtensionEvidenceWith/FromReference
fungal-type cell wall organization56
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum596
Annotation ExtensionEvidenceWith/FromReference
fungal-type vacuole membrane91
Annotation ExtensionEvidenceWith/FromReference
integral component of endoplasmic reticulum membrane68
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationbig1ΔNull1428
sensitive to 4-nitroquinoline N-oxidebig1ΔNull159
sensitive to caffeinebig1ΔNull108
sensitive to camptothecinbig1ΔNull224
sensitive to hydrogen peroxidebig1ΔNull137
sensitive to hydroxyureabig1ΔNull525

Cell Phenotype

Term NameAlleleExpressionCount
abnormal microtubule cytoskeleton organization during vegetative growthbig1+ (wild type)Overexpression283
inviable sporebig1ΔNull468
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons419553..419382, 419319..418005
5' UTR419553..419474PMID:21511999
CDS419473..419382, 419319..418476
3' UTR418475..418005PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF05827 Pfam IPR024722 BIG/ATPase V1 complex, subunit S1 21 295 1
TMhelix TMHMM 271 293 959
SignalP-noTM signalp 1 17 212

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount

Protein Properties

Ave. residue weight 111.37 Da
Charge -10.00
Isoelectric point 4.65
Molecular weight 34.64 kDa
Number of residues 311
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5109.15during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
6.1during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
1.7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi only620
conserved in fungi4604
conserved in eukaryotes4516

Manually curated orthologous groups


Orthologs in Compara

External References
Database Identifier Description
NBRP SPCC306.06c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC306.06c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC306.06c BioGRID Interaction Datasets
Expression Viewer SPCC306.06c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC306.06c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC306.06c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC306.06c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC306.06c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC306.06c Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC306.06c Cell Cycle Data
GEO SPCC306.06c GEO profiles
PInt SPCC306.06c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC306.06c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC306.06c Fission yeast phenotypic data & analysis
SPD / RIKEN14/14F02Orfeome Localization Data
UniProtKB/SwissProtQ9Y7R6Protein big1
ModBaseQ9Y7R6Database of comparative protein structure models
STRINGQ9Y7R6Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587814ER membrane protein, BIG1 family (predicted)
RefSeq mRNANM_001022807972h- ER membrane protein, BIG1 family (predicted) (SPCC306.06c), mRNA
European Nucleotide ArchiveCAB41654.1ENA Protein Mapping
UniParcUPI0000069B7FUniProt Archive

Literature for big1

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015