sds21 (SPCC31H12.05c)


Gene Standard Namesds21 Characterisation Statuspublished
Systematic IDSPCC31H12.05c Feature Typeprotein coding
Synonyms Name Description
Productserine/threonine protein phosphatase PP1 subfamily, Sds21 Product Size322aa, 36.83 kDa
Genomic Location Chromosome III, 631529-628570 (2960nt); CDS:630443-628996 (1448nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
metal ion binding743
Annotation ExtensionEvidenceWith/FromReference
protein serine/threonine phosphatase activity15
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
positive regulation of establishment of cell polarity regulating cell shape5
Annotation ExtensionEvidenceWith/FromReference
protein dephosphorylation31
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nucleolus363
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
protein phosphatase type 1 complex4
Annotation ExtensionEvidenceWith/FromReference
PTW/PP1 phosphatase complex3
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
sensitive to bortezomibsds21ΔNull256
slow vegetative cell population growthsds21ΔNull328
viable vegetative cell populationsds21ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
decreased protein phosphatase activitysds21::LEU2 (disruption)Not specified5
normal protein localization during vegetative growth193
affecting dis2sds21ΔNull
viable vegetative cell with normal cell morphologysds21ΔNull3094
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in dis2 serine/threonine protein phosphatase PP1 subfamily, Dis2
FYPO affected by mutation in sds22 protein phosphatase regulatory subunit Sds22
FYPO affected by mutation in tea4 tip elongation aberrant protein Tea4
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons631529..630838, 630719..630401, 630342..630218, 629847..629146, 629094..628570
Introns630837..630720PMID:18488015
mRNA631529..628570
5' UTR631529..630838, 630719..630444PMID:18488015
CDS630443..630401, 630342..630218, 629847..629146, 629094..628996
3' UTR628995..628570AU010789
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00149 Pfam IPR004843 Calcineurin-like phosphoesterase domain, apaH type 55 246 20
SM00156 SMART IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 26 296 10
PS00125 Prosite Patterns IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 117 122 10
PTHR11668:SF204 HMMPANTHER 2 313 1
PTHR11668 HMMPANTHER 2 313 11
3.60.21.10 Gene3D IPR029052 Metallo-dependent phosphatase-like 6 306 23
SSF56300 SuperFamily IPR029052 Metallo-dependent phosphatase-like 21 299 22
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 236 256 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 54 81 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 179 206 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 150 176 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 258 274 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 83 110 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 116 140 10

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.38 Da
Charge -2.50
Isoelectric point 5.75
Molecular weight 36.83 kDa
Number of residues 322
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS302
present during mitotic M phaseS305
present during mitotic M phaseS304
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S302 PMID:21712547
present during mitotic M phase experimental evidence S304 PMID:21712547
present during mitotic M phase experimental evidence S305 PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
15693.75during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
3180.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.6during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.73during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturescmb1cytosine-mismatch binding protein 1 Affinity Capture-MSPMID:24945319
affinity capturesglc8protein phosphatase regulatory subunit Glc8 (predicted) Affinity Capture-MSPMID:24945319
affinity capturesmcp60mitochondrial heat shock protein Hsp60/Mcp60 Affinity Capture-MSPMID:24945319
affinity capturesrad2414-3-3 protein Rad24 Affinity Capture-MSPMID:24945319
affinity capturesrif1telomere length regulator protein Rif1 Affinity Capture-WesternPMID:24656819
affinity captured bysds22protein phosphatase regulatory subunit Sds22 Affinity Capture-WesternPMID:15335873
affinity capturessds22protein phosphatase regulatory subunit Sds22 Affinity Capture-MSPMID:24945319
affinity capturesSPBC3B8.03saccharopine dehydrogenase (predicted) Affinity Capture-MSPMID:24945319
affinity captured bytea4tip elongation aberrant protein Tea4 Affinity Capture-WesternPMID:24554432
affinity capturestea4tip elongation aberrant protein Tea4 Affinity Capture-MSPMID:24945319
affinity capturesucp3GTPase activating protein Ucp3 (predicted) Affinity Capture-MSPMID:24945319
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
overexpression rescuescdc25M phase inducer tyrosine phosphatase Cdc25 Dosage RescuePMID:1944266
synthetic lethal withdis2serine/threonine protein phosphatase PP1 subfamily, Dis2 Synthetic LethalityPMID:2170029
synthetic lethal withdis2serine/threonine protein phosphatase PP1 subfamily, Dis2 Synthetic LethalityPMID:2544298
overexpression rescuessds22protein phosphatase regulatory subunit Sds22 Dosage RescuePMID:1846086
overexpression rescuessds23PP2A-type phosphatase inhibitor Sds23/Moc1 Dosage RescuePMID:8978689
overexpression rescueswee1M phase inhibitor protein kinase Wee1 Dosage RescuePMID:1944266
External References
Database Identifier Description
NBRP SPCC31H12.05c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC31H12.05c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC31H12.05c BioGRID Interaction Datasets
Expression Viewer SPCC31H12.05c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC31H12.05c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC31H12.05c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC31H12.05c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC31H12.05c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC31H12.05c Transcriptome Viewer (Bähler Lab)
GEO SPCC31H12.05c GEO profiles
PInt SPCC31H12.05c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC31H12.05c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC31H12.05c Fission yeast phenotypic data & analysis
Cyclebase SPCC31H12.05c.1 Cell Cycle Data
SPD / RIKEN23/23C11Orfeome Localization Data
IntEnz3.1.3.16Integrated relational Enzyme database
Rhea3.1.3.16Annotated reactions database
UniProtKB/SwissProtP23880Serine/threonine-protein phosphatase PP1-2
ModBaseP23880Database of comparative protein structure models
STRINGP23880Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587898serine/threonine protein phosphatase Sds21
European Nucleotide ArchiveAAA35341.1ENA Protein Mapping
European Nucleotide ArchiveCAA21222.1ENA Protein Mapping
UniParcUPI0000131FB0UniProt Archive

Literature for sds21

Search: Europe PMC or PubMed

Release Version: PomBase:26_53 - 27 May 2015