sds21 (SPCC31H12.05c)


Gene Standard Namesds21 Characterisation Statuspublished
Systematic IDSPCC31H12.05c Feature Typeprotein coding
Synonyms Name Description
Productserine/threonine protein phosphatase PP1 subfamily, Sds21 Product Size322aa, 36.83 kDa
Genomic Location Chromosome III, 631529-628570 (2960nt); CDS:630443-628996 (1448nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
metal ion binding750
Annotation ExtensionEvidenceWith/FromReference
protein serine/threonine phosphatase activity14
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
nucleolus365
Annotation ExtensionEvidenceWith/FromReference
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
protein phosphatase type 1 complex4
Annotation ExtensionEvidenceWith/FromReference
PTW/PP1 phosphatase complex3
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationsds21+1455
resistance to 5-fluorouracilsds21Δ13
resistance to tamoxifensds21Δ61
sensitive to bortezomibsds21Δ255
slow vegetative cell population growthsds21-TPPR (Dis3-Cterm)367
sds21Δ
viable vegetative cell populationsds21Δ3850

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic cell cycle arrest with replicated DNAsds21-TPPR (Dis3-Cterm)45
decreased protein phosphatase activitysds21::LEU25
sds21Δ
inviable elongated mononucleate aseptate vegetative cell with cell cycle arrest in mitotic G2 phasesds21-TPPR (Dis3-Cterm)7
normal protein localization during vegetative growth276
affecting dis2sds21Δ
viable vegetative cell with normal cell morphologysds21Δ3102
viable vegetative cell with normal cell shapesds21-TPPR (Dis3-Cterm)31

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
inviable vegetative cell populationcdc25-22 (C532Y), wee1-50, sds21+
dis3-54 (P509L), sds21Δ
normal growth on okadaic aciddis1-288, dis3-54 (P509L), dis2Δ, ppe1Δ, dis2-11, sds21Δ, ppa1Δ
normal vegetative cell population growthppa1::ura4+, sds21::LEU2
ppa2::ura4+, sds21::LEU2
slow vegetative cell population growthdis2cs (R245Q), sds21::LEU2
viable vegetative cell populationcdc25-22 (C532Y), wee1-50, sds21+
sds21Δ, ppb1::ura4+
ppe1Δ, sds21Δ
dis3-54 (P509L), sds21Δ
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in dis2 serine/threonine protein phosphatase PP1, Dis2
FYPO affected by mutation in sds22 protein phosphatase regulatory subunit Sds22
FYPO affected by mutation in tea4 tip elongation aberrant protein Tea4
FYPO localization affected by mutation in dis2 serine/threonine protein phosphatase PP1, Dis2
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons631529..630838, 630719..630401, 630342..630218, 629847..629146, 629094..628570
Intron630837..630720, 630400..630343, 630217..629848, 629145..629095
mRNA631529..628570
5' UTR631529..630838, 630719..630444PMID:18488015
CDS630443..630401, 630342..630218, 629847..629146, 629094..628996
3' UTR628995..628570AU010789
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF16891 Pfam IPR031675 Serine-threonine protein phosphatase, N-terminal 5 52 3
PF00149 Pfam IPR004843 Calcineurin-like phosphoesterase domain, ApaH type 56 245 19
SM00156 SMART IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 26 296 10
PS00125 Prosite Patterns IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 117 122 10
PTHR11668 HMMPANTHER 4 300 11
PTHR11668:SF255 HMMPANTHER 4 300 1
3.60.21.10 Gene3D IPR029052 Metallo-dependent phosphatase-like 6 306 23
SSF56300 SuperFamily IPR029052 Metallo-dependent phosphatase-like 21 299 22
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 179 206 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 150 176 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 236 256 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 54 81 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 83 110 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 116 140 10
PR00114 PRINTS IPR006186 Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase 258 274 10

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.38 Da
Charge -2.50
Codon Adaptation Index 0.47
Isoelectric point 5.75
Molecular weight 36.83 kDa
Number of residues 322
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during mitotic M phaseS305
present during mitotic M phaseS304
present during mitotic M phaseS302
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S305 PMID:21712547
present during mitotic M phase experimental evidence S304 PMID:21712547
present during mitotic M phase experimental evidence S302 PMID:21712547
O-phosphorylated residueS305,T313 2457
Annotation ExtensionEvidenceResidueReference
IDA S305,T313 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for sds21 (SPCC31H12.05c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
15693.75during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
3180.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.6during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.73during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPCC31H12.05c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bytea4tip elongation aberrant protein Tea4 Affinity Capture-WesternPMID:24554432
affinity captured bysds22protein phosphatase regulatory subunit Sds22 Affinity Capture-WesternPMID:15335873
affinity capturessds22protein phosphatase regulatory subunit Sds22 Affinity Capture-MSPMID:24945319
affinity capturesrad2414-3-3 protein Rad24 Affinity Capture-MSPMID:24945319
affinity captureslys9saccharopine dehydrogenase (predicted) Affinity Capture-MSPMID:24945319
affinity capturesglc8protein phosphatase regulatory subunit Glc8 (predicted) Affinity Capture-MSPMID:24945319
affinity capturesucp3GTPase activating protein Ucp3 Affinity Capture-MSPMID:24945319
affinity capturestea4tip elongation aberrant protein Tea4 Affinity Capture-MSPMID:24945319
affinity capturesmcp60mitochondrial heat shock protein Hsp60/Mcp60 Affinity Capture-MSPMID:24945319
affinity capturescmb1cytosine-mismatch binding protein 1 Affinity Capture-MSPMID:24945319
affinity capturesrif1telomere length regulator protein Rif1 Affinity Capture-WesternPMID:24656819
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPCC31H12.05c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
overexpression rescueswee1M phase inhibitor protein kinase Wee1 Dosage RescuePMID:1944266
overexpression rescuessds22protein phosphatase regulatory subunit Sds22 Dosage RescuePMID:1846086
overexpression rescuescdc25M phase inducer tyrosine phosphatase Cdc25 Dosage RescuePMID:1944266
overexpression rescuessds23PP2A-type phosphatase inhibitor Sds23/Moc1 Dosage RescuePMID:8978689
synthetic lethal withdis2serine/threonine protein phosphatase PP1, Dis2 Synthetic LethalityPMID:2170029
External References
Database Identifier Description
NBRP SPCC31H12.05c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC31H12.05c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC31H12.05c BioGRID Interaction Datasets
Expression Viewer SPCC31H12.05c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC31H12.05c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC31H12.05c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC31H12.05c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC31H12.05c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC31H12.05c Transcriptome Viewer (Bähler Lab)
GEO SPCC31H12.05c GEO profiles
PInt SPCC31H12.05c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC31H12.05c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC31H12.05c Fission yeast phenotypic data & analysis
Cyclebase SPCC31H12.05c.1 Cell Cycle Data
SPD / RIKEN23/23C11Orfeome Localization Data
IntEnz3.1.3.16Integrated relational Enzyme database
Rhea3.1.3.16Annotated reactions database
UniProtKB/SwissProtP23880Serine/threonine-protein phosphatase PP1-2
ModBaseP23880Database of comparative protein structure models
STRINGP23880Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587898serine/threonine protein phosphatase Sds21
European Nucleotide ArchiveAAA35341.1ENA Protein Mapping
European Nucleotide ArchiveCAA21222.1ENA Protein Mapping
KEGG04660+3.1.3.16T cell receptor signaling pathway
UniParcUPI0000131FB0UniProt Archive

Literature for sds21

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016