ctp1 (SPCC338.08)


Gene Standard Namectp1 Characterisation Statuspublished
Systematic IDSPCC338.08 Feature Typeprotein coding
Synonymsmug38, nip1, slr9 Name DescriptionMeiotically Upregulated Gene,
Synthetic Lethal with Rad2 mutant,
Nbs1-Interacting Protein 1
ProductCtIP-related endonuclease Product Size294aa, 33.10 kDa
Genomic Location Chromosome III, 1360260-1358737 (1524nt); CDS:1359819-1358891 (929nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
DNA binding379
Annotation ExtensionEvidenceWith/FromReference
endonuclease activity77
Annotation ExtensionEvidenceWith/FromReference
single-stranded DNA endodeoxyribonuclease activity7
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
DNA damage checkpoint32
Annotation ExtensionEvidenceWith/FromReference
DNA double-strand break processing4
Annotation ExtensionEvidenceWith/FromReference
double-strand break repair via homologous recombination34
Annotation ExtensionEvidenceWith/FromReference
meiotic DNA double-strand break processing2
Annotation ExtensionEvidenceWith/FromReference
response to ionizing radiation1
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nuclear chromosome453
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
sensitive to 4-nitroquinoline N-oxidectp1ΔNull159
sensitive to amphotericin Bctp1ΔNull69
sensitive to camptothecinctp1ΔNull224
sensitive to clotrimazolectp1ΔNull105
sensitive to doxorubicinctp1ΔNull91
sensitive to hydroxyureactp1ΔNull534
sensitive to ionizing radiationctp1ΔNull58
unnamed (unrecorded)
sensitive to methyl methanesulfonatectp1ΔNull237
sensitive to terbinafinectp1ΔNull102
sensitive to UV during vegetative growthctp1ΔNull141
slow vegetative cell population growthunnamed (unrecorded)326
viable vegetative cell populationctp1ΔNull3795
unnamed (unrecorded)

Cell Phenotype

Term NameAlleleExpressionCount
abnormal DNA damage checkpointctp1ΔNull24
abnormal microtubule cytoskeleton morphology during mitotic interphasectp1ΔNull186
abnormal mitotic cell cyclectp1ΔNull846
decreased protein phosphorylation during mitotic G2 phase during cellular response to ionizing radiation1
affecting chk1ctp1ΔNull
elongated vegetative cellunnamed (unrecorded)760
increased duration of protein localization to double-strand break site1
affecting mre11ctp1ΔNull
increased protein phosphorylation during mitotic G2 phase2
affecting chk1ctp1ΔNull
viable elongated vegetative cellctp1ΔNull289
viable vegetative cell with abnormal cell shapectp1ΔNull478
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in mre11 Mre11 nuclease PMID:23080121
GO localized by mre11 Mre11 nuclease PMID:17936710
GO regulated by cdc10 MBF transcription factor complex subunit Cdc10 PMID:17936710
GO regulated by rep1 MBF transcription factor activator Rep1 PMID:17936710
GO regulated by rep2 MBF transcription factor activator Rep2 PMID:17936710
GO substrate of tel1 ATM checkpoint kinase PMID:18378696
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1360260..1359127, 1359082..1358737
mRNA1360260..1358737
5' UTR1360260..1359820PMID:21511999
CDS1359819..1359127, 1359082..1358891
3' UTR1358890..1358737SPC01163
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08573 Pfam IPR013882 DNA repair protein Sae2/CtIP 209 293 1
Coil ncoils Rabaptin coiled-coil domain 16 44 968

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.59 Da
Charge -6.50
Isoelectric point 5.17
Molecular weight 33.10 kDa
Number of residues 294
Modifications

Protein Modifications

Term NameResidueCount
phosphorylated residue 1926
present during cellular response to DNA damage stimulus
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
present during cellular response to DNA damage stimulus NAS PMID:18378696
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0051321experimental evidencePMID:16303567

Quantitative Gene Expression

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.6during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.72during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Subunit Composition
DescriptionQualifierReferenceCount
homomeric(2) PMID:1793671049
Misc
DescriptionQualifierReferenceCount
localizes to double-strand breaks PMID:179367101
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:18378696432
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
(peptide) binds to proteinnbs1Mre11 complex subunit Nbs1 Protein-peptidePMID:19804756
affinity capturesnbs1Mre11 complex subunit Nbs1 Affinity Capture-WesternPMID:20421724
affinity captured bynbs1Mre11 complex subunit Nbs1 Affinity Capture-WesternPMID:19804755
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withalp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
negative genetic interaction witharp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
negative genetic interaction witharp8actin-like protein, Ino80 complex subunit Arp8 Negative GeneticPMID:22681890
negative genetic interaction withase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:22681890
negative genetic interaction withask1DASH complex subunit Ask1 Negative GeneticPMID:22681890
positive genetic interaction withatb2tubulin alpha 2 Positive GeneticPMID:22681890
positive genetic interaction withatp11mitochondrial F1-ATPase chaperone Atp11 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withcdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
negative genetic interaction withclr1SHREC complex subunit Clr1 Negative GeneticPMID:22681890
negative genetic interaction withclr3histone deacetylase (class II) Clr3 Negative GeneticPMID:22681890
negative genetic interaction withclr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:22681890
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
negative genetic interaction withcph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
phenotype enhanced bycrb2DNA repair protein Rad9 homolog Crb2 Phenotypic EnhancementPMID:21098122
synthetic growth defect withcrb2DNA repair protein Rad9 homolog Crb2 Synthetic Growth DefectPMID:21098122
negative genetic interaction withcsn1COP9/signalosome complex subunit Csn1 Negative GeneticPMID:22681890
negative genetic interaction withcsn2COP9/signalosome complex subunit Csn2 Negative GeneticPMID:22681890
negative genetic interaction withcwf26complexed with Cdc5 protein Cwf26 Negative GeneticPMID:22681890
negative genetic interaction withdad3DASH complex subunit Dad3 Negative GeneticPMID:22681890
negative genetic interaction withdad4DASH complex subunit Dad4 Negative GeneticPMID:22681890
negative genetic interaction withdam1DASH complex subunit Dam1 Negative GeneticPMID:22681890
synthetic growth defect withdcd1deoxycytidylate deaminase (predicted) Synthetic Growth DefectPMID:22927644
negative genetic interaction withddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
negative genetic interaction withduo1DASH complex subunit Duo1 Negative GeneticPMID:22681890
negative genetic interaction withexo1exonuclease I Exo1 Negative GeneticPMID:22681890
negative genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
negative genetic interaction withfin1serine/threonine protein kinase, NIMA related Fin1 Negative GeneticPMID:22681890
negative genetic interaction withgcn20AAA family ATPase Gcn20 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
negative genetic interaction withhrq1RecQ type DNA helicase Hrq1 (predicted) Negative GeneticPMID:22681890
synthetic growth defect withhta2histone H2A beta Synthetic Growth DefectPMID:21098122
phenotype enhanced byhus1checkpoint clamp complex protein Hus1 Phenotypic EnhancementPMID:21098122
synthetic growth defect withhus1checkpoint clamp complex protein Hus1 Synthetic Growth DefectPMID:21098122
negative genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
negative genetic interaction withmug154conserved fungal protein Negative GeneticPMID:22681890
negative genetic interaction withmug55Cdc20/Fizzy subfamily WD repeat protein Negative GeneticPMID:22681890
phenotype enhanced bynbs1Mre11 complex subunit Nbs1 Phenotypic EnhancementPMID:21098122
synthetic growth defect withnbs1Mre11 complex subunit Nbs1 Synthetic Growth DefectPMID:21098122
negative genetic interaction withnrm1MBF complex corepressor Nrm1 Negative GeneticPMID:22681890
negative genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Negative GeneticPMID:22681890
synthetically rescued bypku70Ku domain protein Pku70 Synthetic RescuePMID:21931565
synthetically rescued bypku80Ku domain protein Pku80 Synthetic RescuePMID:21931565
phenotype enhanced bypku80Ku domain protein Pku80 Phenotypic EnhancementPMID:21931565
negative genetic interaction withpnk1DNA kinase/phosphatase Pnk1 Negative GeneticPMID:22681890
negative genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
negative genetic interaction withpus1tRNA pseudouridine synthase Lsp1 Negative GeneticPMID:22681890
negative genetic interaction withrad1checkpoint clamp complex protein Rad1 Negative GeneticPMID:22681890
synthetically rescuesrad3ATR checkpoint kinase Rad3 Synthetic RescuePMID:21098122
synthetically rescued byrad3ATR checkpoint kinase Rad3 Synthetic RescuePMID:21098122
rescuesrad3ATR checkpoint kinase Rad3 Phenotypic SuppressionPMID:21098122
positive genetic interaction withrad51RecA family recombinase Rad51/Rhp51 Positive GeneticPMID:22681890
negative genetic interaction withraf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Negative GeneticPMID:22681890
negative genetic interaction withraf2Rik1-associated factor Raf2 Negative GeneticPMID:22681890
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
negative genetic interaction withres2MBF transcription factor complex subunit Res2 Negative GeneticPMID:22681890
negative genetic interaction withrhp14XP-A family homolog Rhp14 Negative GeneticPMID:22681890
negative genetic interaction withset5histone lysine methyltransferase Set5 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withspc34DASH complex subunit Spc34 Negative GeneticPMID:22681890
negative genetic interaction withSPCC126.01cconserved fungal protein Negative GeneticPMID:22681890
negative genetic interaction withSPCC126.13chistone deacetylase complex subunit, SAP128 family (predicted) Negative GeneticPMID:22681890
negative genetic interaction withspt2non-specific DNA binding protein Spt2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsty1MAP kinase Sty1 Negative GeneticPMID:22681890
negative genetic interaction withswd1Set1C complex subunit Swd1 Negative GeneticPMID:22681890
negative genetic interaction withswi6HP1 family chromodomain protein Swi6 Negative GeneticPMID:22681890
negative genetic interaction withtas3RITS complex subunit 3 Negative GeneticPMID:22681890
rescuestaz1human TRF ortholog Taz1 Phenotypic SuppressionPMID:23335786
phenotype enhanced bytel1ATM checkpoint kinase Phenotypic EnhancementPMID:21098122
positive genetic interaction withtom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
rescuestrt1telomerase reverse transcriptase 1 protein Trt1 Phenotypic SuppressionPMID:23335786
External References
Database Identifier Description
NBRP SPCC338.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC338.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC338.08 BioGRID Interaction Datasets
Expression Viewer SPCC338.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC338.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC338.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC338.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC338.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC338.08 Transcriptome Viewer (Bähler Lab)
GEO SPCC338.08 GEO profiles
PInt SPCC338.08 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC338.08 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC338.08 Fission yeast phenotypic data & analysis
Cyclebase SPCC338.08.1 Cell Cycle Data
SPD / RIKEN11/11D10Orfeome Localization Data
UniProtKB/SwissProtO74986DNA endonuclease ctp1
ModBaseO74986Database of comparative protein structure models
STRINGO74986Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588159CtIP-related endonuclease
RefSeq mRNANM_001023148972h- CtIP-related endonuclease (ctp1), mRNA
European Nucleotide ArchiveAB363065ENA EMBL mapping
European Nucleotide ArchiveEU168806ENA EMBL mapping
ePDB3HUFThe European PDB
PDB3HUFPDB
PDBsum3HUFPDBsum
ePDB4X01The European PDB
PDB4X01PDB
PDBsum4X01PDBsum
European Nucleotide ArchiveCAA19339.2ENA Protein Mapping
UniParcUPI00015FED0CUniProt Archive

Literature for ctp1

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015