wbp1 (SPCC338.15)


Gene Standard Namewbp1 Characterisation Statusbiological_role_inferred
Systematic IDSPCC338.15 Feature Typeprotein coding
Synonyms Name Description
Productdolichyl-di-phosphooligosaccharide-protein glycotransferase subunit Wbp1 (predicted) Product Size437aa, 48.90 kDa
Genomic Location Chromosome III, 1349468-1347604 (1865nt); CDS:1349398-1347822 (1577nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
protein N-linked glycosylation32
Annotation ExtensionEvidenceWith/FromReference
protein N-linked glycosylation via asparagine1
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum594
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane1018
Annotation ExtensionEvidenceWith/FromReference
oligosaccharyltransferase complex8
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopywbp1ΔNullPECO:0000005, PECO:0000137PMID:236978061338
Microscopywbp1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001042inviable after spore germination, single or double cell division97
penetrance FYPO_EXT:0000003Microscopywbp1ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0000313inviable after spore germination, without cell division, with abnormal germ tube morphology146
penetrance FYPO_EXT:0000001Microscopywbp1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
113494681349269
213492221349112
313490241348369
413482381347604

UTRs

Region Coordinates Reference
five_prime_UTR1349468..1349399PMID:21511999
three_prime_UTR1347821..1347604PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03345 Pfam IPR005013 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 29 436 1
TMhelix TMHMM 400 422 959
PTHR10830 HMMPANTHER IPR005013 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 4 437 1
PTHR10830:SF0 HMMPANTHER 4 437 1
SSF52317 SuperFamily Class I glutamine amidotransferase-like 13 138 21
SignalP-noTM signalp 1 19 212

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.90 Da
Charge -7.00
Isoelectric point 5.15
Molecular weight 48.90 kDa
Number of residues 437
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
58438during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
54924during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
52071during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
53621during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
53159during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
24624.74during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
18686.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
6.8during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.63during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
paa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
External References
Database Identifier Description
NBRP SPCC338.15 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC338.15 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC338.15 BioGRID Interaction Datasets
Expression Viewer SPCC338.15 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC338.15 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC338.15 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC338.15 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC338.15 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC338.15 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC338.15 Cell Cycle Data
GEO SPCC338.15 GEO profiles
PInt SPCC338.15 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC338.15 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC338.15 Fission yeast phenotypic data & analysis
SPD / RIKEN25/25D06Orfeome Localization Data
UniProtKB/SwissProtO59866Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit wbp1
ModBaseO59866Database of comparative protein structure models
STRINGO59866Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588153dolichyl-di-phosphooligosaccharide-protein glycotransferase subunit Wbp1 (predicted)
RefSeq mRNANM_001023142972h- dolichyl-di-phosphooligosaccharide-protein glycotransferase subunit Wbp1 (predicted) (wbp1), mRNA
European Nucleotide ArchiveAB015170ENA EMBL mapping
European Nucleotide ArchiveCAA19346.1ENA Protein Mapping
UniParcUPI000006A7D2UniProt Archive

Literature for wbp1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014