wbp1 (SPCC338.15)


Gene Standard Namewbp1 Characterisation Statusbiological_role_inferred
Systematic IDSPCC338.15 Feature Typeprotein coding
Synonyms Name Description
Productdolichyl-di-phosphooligosaccharide-protein glycotransferase subunit Wbp1 (predicted) Product Size437aa, 48.90 kDa
Genomic Location Chromosome III, 1349468-1347604 (1865nt); CDS:1349398-1347822 (1577nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004579dolichyl-diphosphooligosaccharide-protein glycotransferase activityIEAIPR005013GO_REF:00000028
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006487protein N-linked glycosylationISOSGD:S000000728GO_REF:000002429
GO:0018279protein N-linked glycosylation via asparagineIEAIPR005013GO_REF:00000021
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005783endoplasmic reticulumIDAPMID:16823372594
GO:0016021integral component of membraneIEAUniProtKB-KW:KW-0812GO_REF:00000371016
GO:0008250oligosaccharyltransferase complexISOSGD:S000000728GO_REF:00000248
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopywbp1ΔNullPECO:0000005, PECO:0000137PMID:236978061309
Microscopywbp1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001042inviable after spore germination, single or double cell divisionMicroscopywbp1ΔNullPECO:0000005, PECO:0000137PMID:2369780691
penetrance FYPO_EXT:0000003
FYPO:0000313inviable after spore germination, without cell division, with abnormal germ tube morphologyMicroscopywbp1ΔNullPECO:0000005, PECO:0000137PMID:23697806144
penetrance FYPO_EXT:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
113494681349269
213492221349112
313490241348369
413482381347604

UTRs

Region Start End Reference
three_prime_UTR13478211347604PMID:21511999
five_prime_UTR13494681349399PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03345 Pfam IPR005013 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 29 436 1
TMhelix TMHMM 400 422 959
PTHR10830 HMMPANTHER IPR005013 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 4 437 1
PTHR10830:SF0 HMMPANTHER 4 437 1
SSF52317 SuperFamily 13 138 21
Low complexity (SEG) seg 424 435
SignalP-noTM signalp 1 19 212

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.90 Da
Charge -7.00
Isoelectric point 5.15
Molecular weight 48.90 kDa
Number of residues 437
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
24624.74during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
18686.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.63during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
6.8during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
paa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
External References
Database Identifier Description
NBRP SPCC338.15 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC338.15 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC338.15 BioGRID Interaction Datasets
Expression Viewer SPCC338.15 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC338.15 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC338.15 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC338.15 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC338.15 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC338.15 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC338.15 Cell Cycle Data
GEO SPCC338.15 GEO profiles
PInt SPCC338.15 Protein-Protein Interaction Predictor (Bähler Lab)
SPD / RIKEN25/25D06Orfeome Localization Data
WikiGene2539520dolichyl-di-phosphooligosaccharide-protein glycotransferase subunit (predicted)
EntrezGene2539520dolichyl-di-phosphooligosaccharide-protein glycotransferase subunit (predicted)
UniProtKB/SwissProtO59866Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit wbp1
ModBaseO59866Database of comparative protein structure models
StringO59866Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588153dolichyl-di-phosphooligosaccharide-protein glycotransferase subunit (predicted)
RefSeq mRNANM_001023142972h- dolichyl-di-phosphooligosaccharide-protein glycotransferase subunit (predicted) (SPCC338.15), mRNA
European Nucleotide ArchiveBAA28753ENA Protein Mapping
European Nucleotide ArchiveCAA19346ENA Protein Mapping
UniParcUPI000006A7D2UniProt Archive

Literature for wbp1

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014