wbp1 (SPCC338.15)


Gene Standard Namewbp1 Characterisation Statusbiological_role_inferred
Systematic IDSPCC338.15 Feature Typeprotein coding
Synonyms Name Description
Productdolichyl-di-phosphooligosaccharide-protein glycotransferase subunit Wbp1 (predicted) Product Size437aa, 48.90 kDa
Genomic Location Chromosome III, 1349468-1347604 (1865nt); CDS:1349398-1347822 (1577nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
protein N-linked glycosylation32
Annotation ExtensionEvidenceWith/FromReference
protein N-linked glycosylation via asparagine1
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum596
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane1018
Annotation ExtensionEvidenceWith/FromReference
oligosaccharyltransferase complex8
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationwbp1ΔNull1428

Cell Phenotype

Term NameAlleleExpressionCount
inviable after spore germination, single or double cell divisionwbp1ΔNull101
inviable after spore germination, without cell division, with abnormal germ tube morphologywbp1ΔNull151
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1349468..1349269, 1349222..1349112, 1349024..1348369, 1348238..1347604
Introns1348368..1348239
mRNA1349468..1347604
5' UTR1349468..1349399PMID:21511999
CDS1349398..1349269, 1349222..1349112, 1349024..1348369, 1348238..1347822
3' UTR1347821..1347604PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03345 Pfam IPR005013 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 29 436 1
TMhelix TMHMM 400 422 959
PTHR10830 HMMPANTHER IPR005013 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 4 437 1
PTHR10830:SF0 HMMPANTHER 4 437 1
SSF52317 SuperFamily IPR029062 Class I glutamine amidotransferase-like 13 138 21
SignalP-noTM signalp 1 19 212

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.90 Da
Charge -7.00
Isoelectric point 5.15
Molecular weight 48.90 kDa
Number of residues 437
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
58438during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
54924during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
52071during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
53621during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
53159during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
24624.74during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
18686.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
6.8during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.63during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byhhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
affinity captured byhhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
affinity captured bypaa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
External References
Database Identifier Description
NBRP SPCC338.15 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC338.15 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC338.15 BioGRID Interaction Datasets
Expression Viewer SPCC338.15 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC338.15 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC338.15 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC338.15 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC338.15 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC338.15 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC338.15 Cell Cycle Data
GEO SPCC338.15 GEO profiles
PInt SPCC338.15 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC338.15 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC338.15 Fission yeast phenotypic data & analysis
SPD / RIKEN25/25D06Orfeome Localization Data
UniProtKB/SwissProtO59866Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit wbp1
ModBaseO59866Database of comparative protein structure models
STRINGO59866Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588153dolichyl-di-phosphooligosaccharide-protein glycotransferase subunit Wbp1 (predicted)
RefSeq mRNANM_001023142972h- dolichyl-di-phosphooligosaccharide-protein glycotransferase subunit Wbp1 (predicted) (wbp1), mRNA
European Nucleotide ArchiveAB015170ENA EMBL mapping
European Nucleotide ArchiveCAA19346.1ENA Protein Mapping
KEGG_Enzyme00510+2.4.99.18N-Glycan biosynthesis
KEGG_Enzyme00513+2.4.99.18Various types of N-glycan biosynthesis
UniParcUPI000006A7D2UniProt Archive
UniPathwayUPA00378Protein modification; protein glycosylation

Literature for wbp1

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015