tef3 (SPCC417.08)


Gene Standard Nametef3 Characterisation Statuspublished
Systematic IDSPCC417.08 Feature Typeprotein coding
Synonyms Name Description
Producttranslation elongation factor eEF3 Product Size1047aa, 115.80 kDa
Genomic Location Chromosome III, 1685599-1688951 (3353nt); CDS:1685666-1688809 (3144nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037559
GO:0016887ATPase activityISOSGD:S000004959GO_REF:0000024233
GO:0003746translation elongation factor activityISOSGD:S000004959GO_REF:000002415
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0002182cytoplasmic translational elongationNASGO_REF:0000051193
GO:0006414translational elongationISOSGD:S000004959GO_REF:0000024221
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722317
GO:0022626cytosolic ribosome198
colocalizes_withISOSGD:S000004959GO_REF:0000024
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopytef3ΔNullPMID:204732891309
Microscopytef3ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002151inviable sporeMicroscopytef3ΔNullPECO:0000005, PECO:0000137PMID:23697806447
penetrance FYPO_EXT:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
116855991688951

UTRs

Region Start End Reference
five_prime_UTR16855991685665PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00005 Pfam IPR003439 ABC transporter-like 690 932 19
PF00005 Pfam IPR003439 ABC transporter-like 450 577 19
SM00382 SMART IPR003593 AAA+ ATPase domain 459 623 73
SM00382 SMART IPR003593 AAA+ ATPase domain 699 977 73
PS50893 Prosite Profiles IPR003439 ABC transporter-like 411 647 19
PS50893 Prosite Profiles IPR003439 ABC transporter-like 673 1000 19
PS00211 Prosite Patterns IPR017871 ABC transporter, conserved site 904 918 16
PTHR19211:SF5 HMMPANTHER IPR015688 177 1022 2
PTHR19211 HMMPANTHER 177 1022 5
G3DSA:3.40.50.300 Gene3D 877 982 279
G3DSA:3.40.50.300 Gene3D 671 778 279
G3DSA:3.40.50.300 Gene3D 441 518 279
G3DSA:3.40.50.300 Gene3D 524 631 279
G3DSA:1.25.10.10 Gene3D IPR011989 31 400 82
SSF48371 SuperFamily IPR016024 721 805 130
SSF48371 SuperFamily IPR016024 359 437 130
SSF48371 SuperFamily IPR016024 254 327 130
SSF48371 SuperFamily IPR016024 572 645 130
SSF48371 SuperFamily IPR016024 13 219 130
SSF48371 SuperFamily IPR016024 917 954 130
SSF52540 SuperFamily IPR027417 827 982 294
SSF52540 SuperFamily IPR027417 440 624 294
SSF52540 SuperFamily IPR027417 673 735 294
Coil ncoils Rabaptin coiled-coil domain 1011 1032 975
Low complexity (SEG) seg 1026 1038
Low complexity (SEG) seg 25 37
Low complexity (SEG) seg 427 433
Low complexity (SEG) seg 449 467
Low complexity (SEG) seg 1013 1019

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000328HEAT repeatTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000328
PBO:0000539AAA family ATPaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000539

Protein Properties

Ave. residue weight 110.60 Da
Charge -1.00
Isoelectric point 6.46
Molecular weight 115.80 kDa
Number of residues 1047
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residuePMID:182575171192
PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
242286.62during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
58843.83during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
160during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
22during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi only603
conserved in fungi4564
conserved in eukaryotes4482
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
sog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
nak1PAK-related kinase Nak1 Affinity Capture-MSPMID:23462181
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
hsp16heat shock protein Hsp16 Two-hybridPMID:11452028
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
slm9hira protein Slm9 Affinity Capture-MSPMID:20976105
External References
Database Identifier Description
NBRP SPCC417.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC417.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC417.08 BioGRID Interaction Datasets
Expression Viewer SPCC417.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC417.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC417.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC417.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC417.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC417.08 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC417.08 Cell Cycle Data
GEO SPCC417.08 GEO profiles
PInt SPCC417.08 Protein-Protein Interaction Predictor (Bähler Lab)
EntrezGene2539295translation elongation factor eEF3
WikiGene2539295translation elongation factor eEF3
SPD / RIKEN30/30B04Orfeome Localization Data
UniProtKB/SwissProtO94489Elongation factor 3
ModBaseO94489Database of comparative protein structure models
StringO94489Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588285translation elongation factor eEF3
RefSeq mRNANM_001023275972h- translation elongation factor eEF3 (tef3), mRNA
European Nucleotide ArchiveBAA11573ENA Protein Mapping
European Nucleotide ArchiveBAA13887ENA Protein Mapping
European Nucleotide ArchiveBAA33896ENA Protein Mapping
European Nucleotide ArchiveCAA22654ENA Protein Mapping
UniParcUPI0000129C88UniProt Archive

Literature for tef3

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014