tef3 (SPCC417.08)


Gene Standard Nametef3 Characterisation Statuspublished
Systematic IDSPCC417.08 Feature Typeprotein coding
Synonyms Name Description
Producttranslation elongation factor eEF3 Product Size1047aa, 115.80 kDa
Genomic Location Chromosome III, 1685599-1688951 (3353nt); CDS:1685666-1688809 (3144nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding559
Annotation ExtensionEvidenceWith/FromReference
ATPase activity240
Annotation ExtensionEvidenceWith/FromReference
translation elongation factor activity15
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic translational elongation194
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationtef3Δ1453

Cell Phenotype

Term NameGenotypesCount
inviable sporetef3Δ476
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1685599..1688951
5' UTR1685599..1685665PMID:21511999
3' UTR1688810..1688951AU008968
3' UTR1688810..1688951D82575
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00005 Pfam IPR003439 ABC transporter-like 450 577 19
PF00005 Pfam IPR003439 ABC transporter-like 690 932 19
SM00382 SMART IPR003593 AAA+ ATPase domain 459 623 70
SM00382 SMART IPR003593 AAA+ ATPase domain 699 977 70
PS50893 Prosite Profiles IPR003439 ABC transporter-like 411 647 19
PS50893 Prosite Profiles IPR003439 ABC transporter-like 673 1000 19
PS00211 Prosite Patterns IPR017871 ABC transporter, conserved site 904 918 16
PTHR19211 HMMPANTHER 298 773 5
PTHR19211 HMMPANTHER 880 1039 5
PTHR19211:SF5 HMMPANTHER IPR015688 Elongation Factor 3 298 773 1
PTHR19211:SF5 HMMPANTHER IPR015688 Elongation Factor 3 880 1039 1
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 671 778 288
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 877 982 288
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 441 518 288
1.25.10.10 Gene3D IPR011989 Armadillo-like helical 31 400 86
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 524 631 288
SSF48371 SuperFamily IPR016024 Armadillo-type fold 917 954 130
SSF48371 SuperFamily IPR016024 Armadillo-type fold 721 805 130
SSF48371 SuperFamily IPR016024 Armadillo-type fold 13 219 130
SSF48371 SuperFamily IPR016024 Armadillo-type fold 359 437 130
SSF48371 SuperFamily IPR016024 Armadillo-type fold 572 645 130
SSF48371 SuperFamily IPR016024 Armadillo-type fold 254 327 130
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 440 624 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 827 982 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 673 735 294
Coil ncoils Predicted coiled-coil protein (DUF2205) 1011 1031 1057

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000328HEAT repeatTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000328
PBO:0000539AAA family ATPaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000539

Protein Properties

Ave. residue weight 110.60 Da
Charge -1.00
Codon Adaptation Index 0.84
Isoelectric point 6.46
Molecular weight 115.80 kDa
Number of residues 1047
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during mitotic M phase, cellular response to thiabendazoleS1043
present during cellular response to thiabendazoleS632
present during mitotic M phaseS871
present during cellular response to thiabendazoleS768
present during cellular response to thiabendazoleS471
present during cellular response to thiabendazoleS943
present during cellular response to thiabendazoleS783
present during mitotic M phaseS650
S783, S1043
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S1043 PMID:21712547
present during cellular response to thiabendazole IDA S1043 PMID:18257517
present during cellular response to thiabendazole IDA S632 PMID:18257517
present during mitotic M phase experimental evidence S871 PMID:21712547
present during cellular response to thiabendazole IDA S768 PMID:18257517
present during cellular response to thiabendazole IDA S471 PMID:18257517
present during cellular response to thiabendazole IDA S943 PMID:18257517
present during cellular response to thiabendazole IDA S783 PMID:18257517
present during mitotic M phase experimental evidence S650 PMID:21712547
experimental evidence S1043 PMID:24763107
experimental evidence S783 PMID:24763107
phosphorylated residue 1930
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
sumoylated lysine 173
Annotation ExtensionEvidenceResidueReference
IDA PMID:26404184
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for tef3 (SPCC417.08)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
279492during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
288026during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
281109during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
274184during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
242286.62during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
277776during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
58843.83during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
160during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
22during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only618
conserved in fungi4605
conserved in eukaryotes4515
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPCC417.08 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byimp1importin alpha Affinity Capture-MSPMID:25963819
affinity captured bysog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
affinity captured byslm9hira protein Slm9 Affinity Capture-MSPMID:20976105
affinity captured bynak1PAK-related kinase Nak1 Affinity Capture-MSPMID:23462181
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
affinity captured byulp2SUMO deconjugating cysteine peptidase Ulp2 (predicted) Affinity Capture-MSPMID:24818994
binds DNA-binding domain construct withhsp16heat shock protein Hsp16 Two-hybridPMID:11452028
modified bydsk1SR protein-specific kinase Dsk1 Biochemical ActivityPMID:26167880
External References
Database Identifier Description
NBRP SPCC417.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC417.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC417.08 BioGRID Interaction Datasets
Expression Viewer SPCC417.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC417.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC417.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC417.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC417.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC417.08 Transcriptome Viewer (Bähler Lab)
GEO SPCC417.08 GEO profiles
PInt SPCC417.08 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC417.08 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC417.08 Fission yeast phenotypic data & analysis
Cyclebase SPCC417.08.1 Cell Cycle Data
SPD / RIKEN30/30B04Orfeome Localization Data
UniProtKB/SwissProtO94489Elongation factor 3
ModBaseO94489Database of comparative protein structure models
STRINGO94489Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588285translation elongation factor eEF3
RefSeq mRNANM_001023275972h- translation elongation factor eEF3 (tef3), mRNA

Literature for tef3

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016