tef3 (SPCC417.08)


Gene Standard Nametef3 Characterisation Statuspublished
Systematic IDSPCC417.08 Feature Typeprotein coding
Synonyms Name Description
Producttranslation elongation factor eEF3 Product Size1047aa, 115.80 kDa
Genomic Location Chromosome III, 1685599-1688951 (3353nt); CDS:1685666-1688809 (3144nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
ATPase activity236
Annotation ExtensionEvidenceWith/FromReference
translation elongation factor activity15
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic translational elongation193
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
cytosolic ribosome200
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationtef3ΔNull1435

Cell Phenotype

Term NameAlleleExpressionCount
inviable sporetef3ΔNull470
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1685599..1688951
mRNA1685599..1688951
5' UTR1685599..1685665PMID:21511999
CDS1685666..1688809
3' UTR1688810..1688951AU008968
3' UTR1688810..1688951D82575
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00005 Pfam IPR003439 ABC transporter-like 690 932 19
PF00005 Pfam IPR003439 ABC transporter-like 450 577 19
SM00382 SMART IPR003593 AAA+ ATPase domain 459 623 70
SM00382 SMART IPR003593 AAA+ ATPase domain 699 977 70
PS50893 Prosite Profiles IPR003439 ABC transporter-like 673 1000 19
PS50893 Prosite Profiles IPR003439 ABC transporter-like 411 647 19
PS00211 Prosite Patterns IPR017871 ABC transporter, conserved site 904 918 16
PTHR19211:SF5 HMMPANTHER IPR015688 Elongation Factor 3 176 1046 1
PTHR19211 HMMPANTHER 176 1046 5
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 524 631 288
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 441 518 288
1.25.10.10 Gene3D IPR011989 Armadillo-like helical 31 400 86
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 877 982 288
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 671 778 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 673 735 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 440 624 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 827 982 294
SSF48371 SuperFamily IPR016024 Armadillo-type fold 917 954 130
SSF48371 SuperFamily IPR016024 Armadillo-type fold 13 219 130
SSF48371 SuperFamily IPR016024 Armadillo-type fold 572 645 130
SSF48371 SuperFamily IPR016024 Armadillo-type fold 254 327 130
SSF48371 SuperFamily IPR016024 Armadillo-type fold 359 437 130
SSF48371 SuperFamily IPR016024 Armadillo-type fold 721 805 130
Coil ncoils Rabaptin coiled-coil domain 1011 1032 968

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000328HEAT repeatTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000328
PBO:0000539AAA family ATPaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000539

Protein Properties

Ave. residue weight 110.60 Da
Charge -1.00
Isoelectric point 6.46
Molecular weight 115.80 kDa
Number of residues 1047
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during cellular response to thiabendazoleS783
present during mitotic M phaseS650
present during cellular response to thiabendazoleS632
present during mitotic M phase, cellular response to thiabendazoleS1043
S783, S1043
present during mitotic M phaseS871
present during cellular response to thiabendazoleS768
present during cellular response to thiabendazoleS471
present during cellular response to thiabendazoleS943
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S471 PMID:18257517
present during cellular response to thiabendazole IDA S632 PMID:18257517
present during mitotic M phase experimental evidence S650 PMID:21712547
present during cellular response to thiabendazole IDA S768 PMID:18257517
experimental evidence S783 PMID:24763107
present during cellular response to thiabendazole IDA S783 PMID:18257517
present during mitotic M phase experimental evidence S871 PMID:21712547
present during cellular response to thiabendazole IDA S943 PMID:18257517
experimental evidence S1043 PMID:24763107
present during cellular response to thiabendazole IDA S1043 PMID:18257517
present during mitotic M phase experimental evidence S1043 PMID:21712547
phosphorylated residue 1926
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
279492during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
288026during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
281109during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
274184during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
242286.62during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
277776during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
58843.83during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
160during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
22during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only620
conserved in fungi4604
conserved in eukaryotes4516
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withhsp16heat shock protein Hsp16 Two-hybridPMID:11452028
affinity captured bynak1PAK-related kinase Nak1 Affinity Capture-MSPMID:23462181
affinity captured byslm9hira protein Slm9 Affinity Capture-MSPMID:20976105
affinity captured bysog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
affinity captured byulp2SUMO deconjugating cysteine peptidase Ulp2 (predicted) Affinity Capture-MSPMID:24818994
External References
Database Identifier Description
NBRP SPCC417.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC417.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC417.08 BioGRID Interaction Datasets
Expression Viewer SPCC417.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC417.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC417.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC417.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC417.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC417.08 Transcriptome Viewer (Bähler Lab)
GEO SPCC417.08 GEO profiles
PInt SPCC417.08 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC417.08 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC417.08 Fission yeast phenotypic data & analysis
Cyclebase SPCC417.08.1 Cell Cycle Data
SPD / RIKEN30/30B04Orfeome Localization Data
UniProtKB/SwissProtO94489Elongation factor 3
ModBaseO94489Database of comparative protein structure models
STRINGO94489Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588285translation elongation factor eEF3
RefSeq mRNANM_001023275972h- translation elongation factor eEF3 (tef3), mRNA
European Nucleotide ArchiveD82575ENA EMBL mapping
European Nucleotide ArchiveD89226ENA EMBL mapping
European Nucleotide ArchiveBAA33896.1ENA Protein Mapping
European Nucleotide ArchiveCAA22654.1ENA Protein Mapping
UniParcUPI0000129C88UniProt Archive

Literature for tef3

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015