tef3 (SPCC417.08)


Gene Standard Nametef3 Characterisation Statuspublished
Systematic IDSPCC417.08 Feature Typeprotein coding
Synonyms Name Description
Producttranslation elongation factor eEF3 Product Size1047aa, 115.80 kDa
Genomic Location Chromosome III, 1685599-1688951 (3353nt); CDS:1685666-1688809 (3144nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037557
GO:0016887ATPase activityISOSGD:S000004959GO_REF:0000024232
GO:0003746translation elongation factor activityISOSGD:S000004959GO_REF:000002415
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0002182cytoplasmic translational elongationNASGO_REF:0000051193
GO:0006414translational elongationISOSGD:S000004959GO_REF:0000024221
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722316
GO:0022626cytosolic ribosome199
colocalizes_withISOSGD:S000004959GO_REF:0000024
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopytef3ΔNullPMID:204732891331
Microscopytef3ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002151inviable spore459
penetrance FYPO_EXT:0000001Microscopytef3ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
116855991688951

UTRs

Region Coordinates Reference
five_prime_UTR1685599..1685665PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00005 Pfam IPR003439 ABC transporter-like 450 577 19
PF00005 Pfam IPR003439 ABC transporter-like 690 932 19
SM00382 SMART IPR003593 AAA+ ATPase domain 699 977 70
SM00382 SMART IPR003593 AAA+ ATPase domain 459 623 70
PS00211 Prosite Patterns IPR017871 ABC transporter, conserved site 904 918 16
PS50893 Prosite Profiles IPR003439 ABC transporter-like 673 1000 19
PS50893 Prosite Profiles IPR003439 ABC transporter-like 411 647 19
PTHR19211 HMMPANTHER 176 1046 5
PTHR19211:SF5 HMMPANTHER IPR015688 Elongation Factor 3 176 1046 1
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 441 518 288
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 524 631 288
1.25.10.10 Gene3D IPR011989 Armadillo-like helical 31 400 86
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 671 778 288
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 877 982 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 827 982 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 673 735 294
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 440 624 294
SSF48371 SuperFamily IPR016024 Armadillo-type fold 721 805 130
SSF48371 SuperFamily IPR016024 Armadillo-type fold 359 437 130
SSF48371 SuperFamily IPR016024 Armadillo-type fold 572 645 130
SSF48371 SuperFamily IPR016024 Armadillo-type fold 254 327 130
SSF48371 SuperFamily IPR016024 Armadillo-type fold 13 219 130
SSF48371 SuperFamily IPR016024 Armadillo-type fold 917 954 130
Coil ncoils Rabaptin coiled-coil domain 1011 1032 968

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000328HEAT repeatTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000328
PBO:0000539AAA family ATPaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000539

Protein Properties

Ave. residue weight 110.60 Da
Charge -1.00
Isoelectric point 6.46
Molecular weight 115.80 kDa
Number of residues 1047
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS1043PMID:217125471669
present during mitotic M phase
present during mitotic M phaseS871PMID:21712547
present during mitotic M phaseS650PMID:21712547
S783PMID:24763107
S1043PMID:24763107
present during cellular response to thiabendazoleIDAS1043PMID:18257517
present during cellular response to thiabendazoleIDAS632PMID:18257517
present during cellular response to thiabendazoleIDAS471PMID:18257517
present during cellular response to thiabendazoleIDAS783PMID:18257517
present during cellular response to thiabendazoleIDAS768PMID:18257517
present during cellular response to thiabendazoleIDAS943PMID:18257517
MOD:00696phosphorylated residueNASPMID:182575171921
NASPMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
279492during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
288026during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
281109during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
274184during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
277776during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
242286.62during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
58843.83during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
160during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
22during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi only603
conserved in fungi4569
conserved in eukaryotes4482
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
ulp2SUMO deconjugating cysteine peptidase Ulp2 (predicted) Affinity Capture-MSPMID:24818994
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
sog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
nak1PAK-related kinase Nak1 Affinity Capture-MSPMID:23462181
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
hsp16heat shock protein Hsp16 Two-hybridPMID:11452028
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
slm9hira protein Slm9 Affinity Capture-MSPMID:20976105
External References
Database Identifier Description
NBRP SPCC417.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC417.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC417.08 BioGRID Interaction Datasets
Expression Viewer SPCC417.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC417.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC417.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC417.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC417.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC417.08 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC417.08 Cell Cycle Data
GEO SPCC417.08 GEO profiles
PInt SPCC417.08 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC417.08 Peptides identified in tandem mass spectrometry proteomics experiments
EntrezGene2539295translation elongation factor eEF3
WikiGene2539295translation elongation factor eEF3
SPD / RIKEN30/30B04Orfeome Localization Data
UniProtKB/SwissProtO94489Elongation factor 3
ModBaseO94489Database of comparative protein structure models
STRINGO94489Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588285translation elongation factor eEF3
RefSeq mRNANM_001023275972h- translation elongation factor eEF3 (tef3), mRNA
European Nucleotide ArchiveD82575ENA EMBL mapping
European Nucleotide ArchiveD89226ENA EMBL mapping
European Nucleotide ArchiveBAA11573ENA Protein Mapping
European Nucleotide ArchiveBAA13887ENA Protein Mapping
European Nucleotide ArchiveBAA33896ENA Protein Mapping
European Nucleotide ArchiveBAA33896.1ENA Protein Mapping
European Nucleotide ArchiveCAA22654ENA Protein Mapping
European Nucleotide ArchiveCAA22654.1ENA Protein Mapping
UniParcUPI0000129C88UniProt Archive

Literature for tef3

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014