mdj1 (SPCC4G3.14)


Gene Standard Namemdj1 Characterisation Statusbiological_role_inferred
Systematic IDSPCC4G3.14 Feature Typeprotein coding
Synonyms Name Description
Productmitochondrial DNAJ domain protein Mdj1 (predicted) Product Size528aa, 57.19 kDa
Genomic Location Chromosome III, 445260-442924 (2337nt); CDS:444756-443170 (1587nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAIPR012724GO_REF:0000002558
GO:0030544Hsp70 protein bindingISMPFAM:PF00226GO_REF:000000126
GO:0046872metal ion bindingIEAUniProtKB-KW:KW-0479GO_REF:0000037748
GO:0051082unfolded protein bindingISOSGD:S000001878GO_REF:000002442
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0034605cellular response to heatISOSGD:S000001878GO_REF:000002430
GO:0000002mitochondrial genome maintenanceISOSGD:S000001878GO_REF:000002435
GO:0042026protein refoldingISOSGD:S000001878GO_REF:00000246
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005743mitochondrial inner membraneISOSGD:S000001878GO_REF:0000024156
GO:0005739mitochondrionIDAPMID:16823372758
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopymdj1ΔNullPMID:204732891337
Microscopymdj1ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0000088sensitive to hydroxyureaCell growth assaymdj1ΔNullPECO:0000137, PECO:0000005PMID:23173672513
FYPO:0000089sensitive to methyl methanesulfonateCell growth assaymdj1ΔNullPECO:0000137, PECO:0000005PMID:23173672214
FYPO:0000091sensitive to thiabendazolecompetitive growth assay evidencemdj1ΔNullPMID:20537132175

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002111inviable tapered vegetative cellMicroscopymdj1ΔNullPECO:0000005, PECO:0000137PMID:23697806134
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1445260442924
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00226 Pfam IPR001623 DnaJ domain 86 147 24
PF00684 Pfam IPR001305 Heat shock protein DnaJ, cysteine-rich domain 240 303 4
PF01556 Pfam IPR002939 Chaperone DnaJ, C-terminal 368 445 5
SM00271 SMART IPR001623 DnaJ domain 85 142 23
PS50076 Prosite Profiles IPR001623 DnaJ domain 86 150 24
PS51188 Prosite Profiles IPR001305 Heat shock protein DnaJ, cysteine-rich domain 227 308 4
PS00636 Prosite Patterns IPR018253 DnaJ domain, conserved site 127 146 14
PTHR24076:SF59 HMMPANTHER 47 510 1
PTHR24076 HMMPANTHER 47 510 9
1.10.287.110 Gene3D IPR001623 DnaJ domain 78 171 26
2.60.260.20 Gene3D 210 234 5
2.60.260.20 Gene3D 307 357 5
2.10.230.10 Gene3D IPR001305 Heat shock protein DnaJ, cysteine-rich domain 235 306 4
2.60.260.20 Gene3D 359 437 5
SSF49493 SuperFamily IPR008971 HSP40/DnaJ peptide-binding 309 367 5
SSF49493 SuperFamily IPR008971 HSP40/DnaJ peptide-binding 210 245 5
SSF46565 SuperFamily IPR001623 DnaJ domain 81 182 25
SSF57938 SuperFamily IPR001305 Heat shock protein DnaJ, cysteine-rich domain 227 307 4
SSF49493 SuperFamily IPR008971 HSP40/DnaJ peptide-binding 362 447 5
PR00625 PRINTS IPR001623 DnaJ domain 122 142 21
PR00625 PRINTS IPR001623 DnaJ domain 88 106 21
PR00625 PRINTS IPR001623 DnaJ domain 106 121 21
PR00625 PRINTS IPR001623 DnaJ domain 142 161 21

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.31 Da
Charge 27.00
Isoelectric point 9.83
Molecular weight 57.19 kDa
Number of residues 528
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
15421during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
15148during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
14550during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
14458during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
15028during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
7017.37during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
3028.28during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.8during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.57during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
External References
Database Identifier Description
NBRP SPCC4G3.14 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC4G3.14 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC4G3.14 BioGRID Interaction Datasets
Expression Viewer SPCC4G3.14 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC4G3.14 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC4G3.14 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC4G3.14 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC4G3.14 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC4G3.14 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC4G3.14 Cell Cycle Data
GEO SPCC4G3.14 GEO profiles
PInt SPCC4G3.14 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC4G3.14 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN25/25E10Orfeome Localization Data
UniProtKB/SwissProtP87239DnaJ homolog 1, mitochondrial
ModBaseP87239Database of comparative protein structure models
STRINGP87239Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587824mitochondrial DNAJ domain protein Mdj1 (predicted)
RefSeq mRNANM_001022817972h- mitochondrial DNAJ domain protein Mdj1 (predicted) (mdj1), mRNA
European Nucleotide ArchiveCAB09769.1ENA Protein Mapping
UniParcUPI0000069F2DUniProt Archive

Literature for mdj1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014