mdj1 (SPCC4G3.14)


Gene Standard Namemdj1 Characterisation Statusbiological_role_inferred
Systematic IDSPCC4G3.14 Feature Typeprotein coding
Synonyms Name Description
Productmitochondrial DNAJ domain protein Mdj1 (predicted) Product Size528aa, 57.19 kDa
Genomic Location Chromosome III, 445260-442924 (2337nt); CDS:444756-443170 (1587nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
Hsp70 protein binding26
Annotation ExtensionEvidenceWith/FromReference
metal ion binding745
Annotation ExtensionEvidenceWith/FromReference
unfolded protein binding41
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
mitochondrial genome maintenance27
Annotation ExtensionEvidenceWith/FromReference
protein refolding6
Annotation ExtensionEvidenceWith/FromReference
response to heat8
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
mitochondrial inner membrane164
Annotation ExtensionEvidenceWith/FromReference
mitochondrion761
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationmdj1+ (wild type)Overexpression1435
mdj1ΔNull
sensitive to hydroxyureamdj1ΔNull534
sensitive to methyl methanesulfonatemdj1ΔNull237
sensitive to thiabendazolemdj1ΔNull211

Cell Phenotype

Term NameAlleleExpressionCount
inviable tapered vegetative cellmdj1ΔNull134
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons445260..442924
mRNA445260..442924
5' UTR445260..444757PMID:21511999
CDS444756..443170
3' UTR443169..442924PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00226 Pfam IPR001623 DnaJ domain 86 147 24
PF00684 Pfam IPR001305 Heat shock protein DnaJ, cysteine-rich domain 240 303 4
PF01556 Pfam IPR002939 Chaperone DnaJ, C-terminal 368 445 5
SM00271 SMART IPR001623 DnaJ domain 85 142 23
PS50076 Prosite Profiles IPR001623 DnaJ domain 86 150 24
PS51188 Prosite Profiles IPR001305 Heat shock protein DnaJ, cysteine-rich domain 227 308 4
PS00636 Prosite Patterns IPR018253 DnaJ domain, conserved site 127 146 14
PTHR24076 HMMPANTHER 69 495 7
PTHR24076:SF5 HMMPANTHER 69 495 1
2.60.260.20 Gene3D 210 234 5
2.60.260.20 Gene3D 307 357 5
1.10.287.110 Gene3D IPR001623 DnaJ domain 78 171 26
2.60.260.20 Gene3D 359 437 5
2.10.230.10 Gene3D IPR001305 Heat shock protein DnaJ, cysteine-rich domain 235 306 4
SSF57938 SuperFamily IPR001305 Heat shock protein DnaJ, cysteine-rich domain 227 307 4
SSF46565 SuperFamily IPR001623 DnaJ domain 81 182 25
SSF49493 SuperFamily IPR008971 HSP40/DnaJ peptide-binding 309 367 5
SSF49493 SuperFamily IPR008971 HSP40/DnaJ peptide-binding 362 447 5
SSF49493 SuperFamily IPR008971 HSP40/DnaJ peptide-binding 210 245 5
MF_01152 hamap IPR012724 Chaperone DnaJ 83 513 3
PR00625 PRINTS IPR001623 DnaJ domain 122 142 21
PR00625 PRINTS IPR001623 DnaJ domain 88 106 21
PR00625 PRINTS IPR001623 DnaJ domain 142 161 21
PR00625 PRINTS IPR001623 DnaJ domain 106 121 21

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.31 Da
Charge 27.00
Isoelectric point 9.83
Molecular weight 57.19 kDa
Number of residues 528
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
15421during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
15148during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
14550during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
14458during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
7017.37during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
15028during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3028.28during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.8during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.57during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


External References
Database Identifier Description
NBRP SPCC4G3.14 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC4G3.14 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC4G3.14 BioGRID Interaction Datasets
Expression Viewer SPCC4G3.14 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC4G3.14 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC4G3.14 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC4G3.14 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC4G3.14 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC4G3.14 Transcriptome Viewer (Bähler Lab)
GEO SPCC4G3.14 GEO profiles
PInt SPCC4G3.14 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC4G3.14 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC4G3.14 Fission yeast phenotypic data & analysis
Cyclebase SPCC4G3.14.1 Cell Cycle Data
SPD / RIKEN25/25E10Orfeome Localization Data
UniProtKB/SwissProtP87239DnaJ homolog 1, mitochondrial
ModBaseP87239Database of comparative protein structure models
STRINGP87239Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587824mitochondrial DNAJ domain protein Mdj1 (predicted)
RefSeq mRNANM_001022817972h- mitochondrial DNAJ domain protein Mdj1 (predicted) (mdj1), mRNA
European Nucleotide ArchiveCAB09769.1ENA Protein Mapping
UniParcUPI0000069F2DUniProt Archive

Literature for mdj1

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015