mdj1 (SPCC4G3.14)


Gene Standard Namemdj1 Characterisation Statusbiological_role_inferred
Systematic IDSPCC4G3.14 Feature Typeprotein coding
Synonyms Name Description
Productmitochondrial DNAJ domain protein Mdj1 (predicted) Product Size528aa, 57.19 kDa
Genomic Location Chromosome III, 445260-442924 (2337nt); CDS:444756-443170 (1587nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAIPR012724GO_REF:0000002559
GO:0030544Hsp70 protein bindingISMPFAM:PF00226GO_REF:000000126
GO:0046872metal ion bindingIEAUniProtKB-KW:KW-0479GO_REF:0000037747
GO:0051082unfolded protein bindingISOSGD:S000001878GO_REF:000002442
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0034605cellular response to heatISOSGD:S000001878GO_REF:000002443
GO:0000002mitochondrial genome maintenanceISOSGD:S000001878GO_REF:000002437
GO:0042026protein refoldingISOSGD:S000001878GO_REF:00000246
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005743mitochondrial inner membraneISOSGD:S000001878GO_REF:0000024155
GO:0005739mitochondrionIDAPMID:16823372756
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopymdj1ΔNullPMID:204732891309
Microscopymdj1ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0000091sensitive to thiabendazolecompetitive growth assay evidencemdj1ΔNullPMID:2053713292

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002111inviable bottle-shaped vegetative cellMicroscopymdj1ΔNullPECO:0000005, PECO:0000137PMID:23697806134
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1445260442924

UTRs

Region Start End Reference
three_prime_UTR443169442924PMID:21511999
five_prime_UTR445260444757PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00226 Pfam IPR001623 DnaJ domain 86 147 24
PF00684 Pfam IPR001305 Heat shock protein DnaJ, cysteine-rich domain 240 303 4
PF01556 Pfam IPR002939 Chaperone DnaJ, C-terminal 368 445 5
SM00271 SMART IPR001623 DnaJ domain 85 142 25
PS00636 Prosite Patterns IPR018253 DnaJ domain 127 146 14
PS50076 Prosite Profiles IPR001623 DnaJ domain 86 150 24
PS51188 Prosite Profiles IPR001305 Heat shock protein DnaJ, cysteine-rich domain 227 308 4
PTHR24076:SF59 HMMPANTHER 47 510 1
PTHR24076 HMMPANTHER 47 510 9
G3DSA:2.60.260.20 Gene3D 359 437 5
G3DSA:2.60.260.20 Gene3D 307 357 5
G3DSA:2.60.260.20 Gene3D 210 234 5
G3DSA:1.10.287.110 Gene3D IPR001623 78 171 26
G3DSA:2.10.230.10 Gene3D IPR001305 235 306 4
SSF57938 SuperFamily IPR001305 227 307 4
SSF46565 SuperFamily IPR001623 81 182 25
SSF49493 SuperFamily IPR008971 309 367 5
SSF49493 SuperFamily IPR008971 362 447 5
SSF49493 SuperFamily IPR008971 210 245 5
Low complexity (SEG) seg 94 108
Low complexity (SEG) seg 166 186
Low complexity (SEG) seg 233 247
Low complexity (SEG) seg 284 289
Low complexity (SEG) seg 462 488
PR00625 PRINTS IPR001623 DnaJ domain 142 161 21
PR00625 PRINTS IPR001623 DnaJ domain 122 142 21
PR00625 PRINTS IPR001623 DnaJ domain 88 106 21
PR00625 PRINTS IPR001623 DnaJ domain 106 121 21

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.31 Da
Charge 27.00
Isoelectric point 9.83
Molecular weight 57.19 kDa
Number of residues 528
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
7017.37during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
3028.28during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.57during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
2.8during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
External References
Database Identifier Description
NBRP SPCC4G3.14 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC4G3.14 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC4G3.14 BioGRID Interaction Datasets
Expression Viewer SPCC4G3.14 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC4G3.14 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC4G3.14 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC4G3.14 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC4G3.14 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC4G3.14 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC4G3.14 Cell Cycle Data
GEO SPCC4G3.14 GEO profiles
PInt SPCC4G3.14 Protein-Protein Interaction Predictor (Bähler Lab)
SPD / RIKEN25/25E10Orfeome Localization Data
WikiGene2539501DNAJ domain protein Mdj1 (predicted)
EntrezGene2539501DNAJ domain protein Mdj1 (predicted)
UniProtKB/SwissProtP87239DnaJ homolog 1, mitochondrial
ModBaseP87239Database of comparative protein structure models
StringP87239Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587824DNAJ domain protein Mdj1 (predicted)
RefSeq mRNANM_001022817972h- DNAJ domain protein Mdj1 (predicted) (mdj1), mRNA
European Nucleotide ArchiveCAB09769ENA Protein Mapping
UniParcUPI0000069F2DUniProt Archive

Literature for mdj1

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014