mdj1 (SPCC4G3.14)

Gene Standard Namemdj1 Characterisation Statusbiological_role_inferred
Systematic IDSPCC4G3.14 Feature Typeprotein coding
Synonyms Name Description
Productmitochondrial DNAJ domain protein Mdj1 (predicted) Product Size528aa, 57.19 kDa
Genomic Location Chromosome III, 445260-442924 (2337nt); CDS:444756-443170 (1587nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
Hsp70 protein binding26
Annotation ExtensionEvidenceWith/FromReference
metal ion binding747
Annotation ExtensionEvidenceWith/FromReference
unfolded protein binding42
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cellular response to heat26
Annotation ExtensionEvidenceWith/FromReference
mitochondrial genome maintenance34
Annotation ExtensionEvidenceWith/FromReference
protein refolding6
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
mitochondrial inner membrane158
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopymdj1ΔNullPECO:0000005, PECO:0000137PMID:236978061338
FYPO:0000088sensitive to hydroxyureaCell growth assaymdj1ΔNullPECO:0000137, PECO:0000005PMID:23173672514
FYPO:0000089sensitive to methyl methanesulfonateCell growth assaymdj1ΔNullPECO:0000137, PECO:0000005PMID:23173672219
FYPO:0000091sensitive to thiabendazolecompetitive growth assay evidencemdj1ΔNullPMID:20537132176

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002111inviable tapered vegetative cellMicroscopymdj1ΔNullPECO:0000005, PECO:0000137PMID:23697806134
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00226 Pfam IPR001623 DnaJ domain 86 147 24
PF00684 Pfam IPR001305 Heat shock protein DnaJ, cysteine-rich domain 240 303 4
PF01556 Pfam IPR002939 Chaperone DnaJ, C-terminal 368 445 5
SM00271 SMART IPR001623 DnaJ domain 85 142 23
PS50076 Prosite Profiles IPR001623 DnaJ domain 86 150 24
PS51188 Prosite Profiles IPR001305 Heat shock protein DnaJ, cysteine-rich domain 227 308 4
PS00636 Prosite Patterns IPR018253 DnaJ domain, conserved site 127 146 14
PTHR24076:SF59 HMMPANTHER 47 510 1
PTHR24076 HMMPANTHER 47 510 9 Gene3D IPR001623 DnaJ domain 78 171 26 Gene3D 210 234 5 Gene3D 307 357 5 Gene3D IPR001305 Heat shock protein DnaJ, cysteine-rich domain 235 306 4 Gene3D 359 437 5
SSF46565 SuperFamily IPR001623 DnaJ domain 81 182 25
SSF49493 SuperFamily IPR008971 HSP40/DnaJ peptide-binding 309 367 5
SSF49493 SuperFamily IPR008971 HSP40/DnaJ peptide-binding 210 245 5
SSF57938 SuperFamily IPR001305 Heat shock protein DnaJ, cysteine-rich domain 227 307 4
SSF49493 SuperFamily IPR008971 HSP40/DnaJ peptide-binding 362 447 5
PR00625 PRINTS IPR001623 DnaJ domain 122 142 21
PR00625 PRINTS IPR001623 DnaJ domain 88 106 21
PR00625 PRINTS IPR001623 DnaJ domain 106 121 21
PR00625 PRINTS IPR001623 DnaJ domain 142 161 21

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.31 Da
Charge 27.00
Isoelectric point 9.83
Molecular weight 57.19 kDa
Number of residues 528
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
15421during GO:0000080PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
15148during GO:0000084PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
14550during GO:0000085PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
14458during GO:0000087PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
15028during GO:0072690PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
7017.37during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
3028.28during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.8during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
0.57during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPCC4G3.14 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC4G3.14 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC4G3.14 BioGRID Interaction Datasets
Expression Viewer SPCC4G3.14 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC4G3.14 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC4G3.14 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC4G3.14 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC4G3.14 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC4G3.14 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC4G3.14 Cell Cycle Data
GEO SPCC4G3.14 GEO profiles
PInt SPCC4G3.14 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC4G3.14 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC4G3.14 Fission yeast phenotypic data & analysis
SPD / RIKEN25/25E10Orfeome Localization Data
UniProtKB/SwissProtP87239DnaJ homolog 1, mitochondrial
ModBaseP87239Database of comparative protein structure models
STRINGP87239Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587824mitochondrial DNAJ domain protein Mdj1 (predicted)
RefSeq mRNANM_001022817972h- mitochondrial DNAJ domain protein Mdj1 (predicted) (mdj1), mRNA
European Nucleotide ArchiveCAB09769.1ENA Protein Mapping
UniParcUPI0000069F2DUniProt Archive

Literature for mdj1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014