alp16 (SPCC4G3.19)


Gene Standard Namealp16 Characterisation Statuspublished
Systematic IDSPCC4G3.19 Feature Typeprotein coding
Synonyms Name Description
Productgamma tubulin complex subunit Alp16 Product Size759aa, 87.24 kDa
Genomic Location Chromosome III, 435465-432264 (3202nt); CDS:434674-432395 (2280nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
interphase microtubule nucleation by interphase microtubule organizing center6
Annotation ExtensionEvidenceWith/FromReference
microtubule cytoskeleton organization110
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
gamma-tubulin complex8
Annotation ExtensionEvidenceWith/FromReference
gamma-tubulin large complex1
Annotation ExtensionEvidenceWith/FromReference
gamma-tubulin large complex, equatorial microtubule organizing center1
Annotation ExtensionEvidenceWith/FromReference
gamma-tubulin small complex2
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle pole body216
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
sensitive to thiabendazolealp16ΔNull218
slow vegetative cell population growthalp16ΔNull327
viable vegetative cell populationalp16ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
curved vegetative cellalp16+ (wild type)Overexpression112
inviable vegetative cellalp16ΔNull1279
long curved cytoplasmic microtubulesalp16+ (wild type)Overexpression2
alp16ΔNull
monopolar mitotic spindlealp16+ (wild type)Overexpression6
normal gamma-tubulin complex assemblyalp16ΔNull2
normal mitosisalp16ΔNull4
T-shaped vegetative cellalp16+ (wild type)Overexpression11
viable vegetative cell with normal cell morphologyalp16ΔNull3093
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons435465..432264
mRNA435465..432264
5' UTR435465..434675PMID:21511999
CDS434674..432395
3' UTR432394..432264PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF04130 Pfam IPR007259 Gamma-tubulin complex component protein 192 665 4

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.94 Da
Charge -8.50
Isoelectric point 5.20
Molecular weight 87.24 kDa
Number of residues 759
Sequence
Gene Expression

Quantitative Gene Expression

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.1during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog600
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesalp4gamma tubulin complex Spc97/GCP2 subunit Alp4 Affinity Capture-WesternPMID:21444751
affinity captured byalp4gamma tubulin complex Spc97/GCP2 subunit Alp4 Affinity Capture-MSPMID:15004232
affinity captured byalp4gamma tubulin complex Spc97/GCP2 subunit Alp4 Affinity Capture-WesternPMID:17021256
affinity captured byalp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Affinity Capture-MSPMID:15004232
affinity capturesgfh1gamma tubulin complex subunit Gfh1 Affinity Capture-WesternPMID:17021256
affinity capturesgtb1gamma-tubulin Gtb1 Affinity Capture-WesternPMID:12134075
affinity capturesmod21gamma tubulin complex subunit Mod21 Affinity Capture-WesternPMID:17021256
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withalp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
synthetic lethal withalp4gamma tubulin complex Spc97/GCP2 subunit Alp4 Synthetic LethalityPMID:12134075
overexpression rescuesalp4gamma tubulin complex Spc97/GCP2 subunit Alp4 Dosage RescuePMID:12134075
synthetic lethal withalp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Synthetic LethalityPMID:12134075
overexpression rescuesalp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Dosage RescuePMID:12134075
negative genetic interaction witharp6actin-like protein Arp6 Negative GeneticPMID:22681890
negative genetic interaction withash2Ash2-trithorax family protein Negative GeneticPMID:22681890
negative genetic interaction withask1DASH complex subunit Ask1 Negative GeneticPMID:18818364
negative genetic interaction withask1DASH complex subunit Ask1 Negative GeneticPMID:22681890
negative genetic interaction withatp15F0-ATPase epsilon subunit (predicted) Negative GeneticPMID:22681890
negative genetic interaction withbdf2BET family double bromodomain protein Bdf2 Negative GeneticPMID:18818364
negative genetic interaction withbob1prefoldin subunit 5 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:18818364
negative genetic interaction withcsr102sec14 cytosolic factor family phosphatidylinositol transmembrane transporter (predicted) Negative GeneticPMID:22681890
negative genetic interaction withctf18RFC-like complex subunit Ctf18 Negative GeneticPMID:18818364
negative genetic interaction withctf18RFC-like complex subunit Ctf18 Negative GeneticPMID:22681890
negative genetic interaction withdad2DASH complex subunit Dad2 Negative GeneticPMID:18818364
negative genetic interaction withdad2DASH complex subunit Dad2 Negative GeneticPMID:22681890
negative genetic interaction withdad3DASH complex subunit Dad3 Negative GeneticPMID:22681890
negative genetic interaction withdad4DASH complex subunit Dad4 Negative GeneticPMID:22681890
negative genetic interaction withdam1DASH complex subunit Dam1 Negative GeneticPMID:22681890
negative genetic interaction withddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
negative genetic interaction withduo1DASH complex subunit Duo1 Negative GeneticPMID:22681890
negative genetic interaction witherp2COPII-coated vesicle component Erp2/3/4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction witheso1sister chromatid cohesion protein/DNA polymerase eta Eso1 fusion protein Negative GeneticPMID:23050226
negative genetic interaction withexg3glucan 1,3-beta-glucosidase Exg3 Negative GeneticPMID:22681890
negative genetic interaction withfin1serine/threonine protein kinase, NIMA related Fin1 Negative GeneticPMID:18818364
negative genetic interaction withfin1serine/threonine protein kinase, NIMA related Fin1 Negative GeneticPMID:22681890
negative genetic interaction withget1GET complex subunit Get1 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withgim1prefoldin subunit 6, Gim1 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withgit3G-protein coupled receptor Git3 Negative GeneticPMID:22681890
negative genetic interaction withgit5heterotrimeric G protein beta subunit Git5 Negative GeneticPMID:22681890
negative genetic interaction withhmt2sulfide-quinone oxidoreductase Negative GeneticPMID:22681890
negative genetic interaction withklp6kinesin-like protein Klp6 Negative GeneticPMID:18818364
negative genetic interaction withklp6kinesin-like protein Klp6 Negative GeneticPMID:22681890
negative genetic interaction withlsg1Lsk1 complex gamma subunit Lsg1 Negative GeneticPMID:22681890
negative genetic interaction withmad2mitotic spindle checkpoint protein Mad2 Negative GeneticPMID:22681890
negative genetic interaction withmga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmph1dual specificity protein kinase Mph1 Negative GeneticPMID:18818364
negative genetic interaction withmph1dual specificity protein kinase Mph1 Negative GeneticPMID:22681890
positive genetic interaction withmrpl1mitochondrial ribosomal protein subunit L1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withmsc1multi-copy suppressor of Chk1 Negative GeneticPMID:18818364
negative genetic interaction withmsc1multi-copy suppressor of Chk1 Negative GeneticPMID:22681890
negative genetic interaction withntp1alpha,alpha-trehalase Ntp1 Negative GeneticPMID:22681890
negative genetic interaction withpac10prefoldin subunit 3 Pac10 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withpac10prefoldin subunit 3 Pac10 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:18818364
negative genetic interaction withpht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:22681890
positive genetic interaction withprp1U4/U6 x U5 tri-snRNP complex subunit Prp1 Positive GeneticPMID:22681890
negative genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
negative genetic interaction withpsh3ER chaperone SHR3 homologue Psh3 Negative GeneticPMID:22681890
negative genetic interaction withrpa12DNA-directed RNA polymerase complex I subunit Rpa12 Negative GeneticPMID:22681890
positive genetic interaction withsnr13-hydroxyisobutyryl-CoA hydrolase snr1 Positive GeneticPMID:22681890
negative genetic interaction withSPAP8A3.07cphospho-2-dehydro-3-deoxyheptonate aldolase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC36B7.08cnucleosome assembly protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withspc19DASH complex subunit Spc19 Negative GeneticPMID:22681890
negative genetic interaction withspc34DASH complex subunit Spc34 Negative GeneticPMID:18818364
negative genetic interaction withspc34DASH complex subunit Spc34 Negative GeneticPMID:22681890
negative genetic interaction withsre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
negative genetic interaction withswc2Swr1 complex complex subunit Swc2 Negative GeneticPMID:18818364
negative genetic interaction withswc2Swr1 complex complex subunit Swc2 Negative GeneticPMID:22681890
negative genetic interaction withswc5Swr1 complex subunit Swc5 Negative GeneticPMID:22681890
negative genetic interaction withswr1SNF2 family ATP-dependent DNA helicase Swr1 Negative GeneticPMID:22681890
positive genetic interaction withtom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withtpp1trehalose-6-phosphate phosphatase Tpp1 Negative GeneticPMID:22681890
negative genetic interaction withyaf9YEATS family histone acetyltransferase subunit Yaf9 Negative GeneticPMID:18818364
External References
Database Identifier Description
NBRP SPCC4G3.19 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC4G3.19 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC4G3.19 BioGRID Interaction Datasets
Expression Viewer SPCC4G3.19 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC4G3.19 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC4G3.19 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC4G3.19 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC4G3.19 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC4G3.19 Transcriptome Viewer (Bähler Lab)
GEO SPCC4G3.19 GEO profiles
PInt SPCC4G3.19 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC4G3.19 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC4G3.19 Fission yeast phenotypic data & analysis
Cyclebase SPCC4G3.19.1 Cell Cycle Data
SPD / RIKEN28/28B12Orfeome Localization Data
UniProtKB/SwissProtP87244Spindle pole body component alp16
ModBaseP87244Database of comparative protein structure models
STRINGP87244Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587819gamma tubulin complex subunit Alp16
RefSeq mRNANM_001022812972h- gamma tubulin complex subunit Alp16 (alp16), mRNA
European Nucleotide ArchiveBAB79248.1ENA Protein Mapping
European Nucleotide ArchiveCAB09763.1ENA Protein Mapping
UniParcUPI0000125798UniProt Archive

Literature for alp16

Search: Europe PMC or PubMed

Release Version: PomBase:26_52 - 11 May 2015