alp16 (SPCC4G3.19)

Gene Standard Namealp16 Characterisation Statuspublished
Systematic IDSPCC4G3.19 Feature Typeprotein coding
Synonyms Name Description
Productgamma tubulin complex subunit Alp16 Product Size759aa, 87.24 kDa
Genomic Location Chromosome III, 435465-432264 (3202nt); CDS:434674-432395 (2280nt)

Ensembl Gene Location
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0051415interphase microtubule nucleation by interphase microtubule organizing center6
GO:0000226microtubule cytoskeleton organization105
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000923equatorial microtubule organizing centerIDAPMID:1213407511
GO:0000930gamma-tubulin complexIDAPMID:121340758
GO:0044732mitotic spindle pole bodyIDAPMID:16823372214
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopyalp16ΔNullPMID:204732893751
Microscopyalp16ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000233long cytoplasmic microtubulesalp16ΔNullPMID:121340752
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopyalp16ΔNullPECO:0000005, PECO:0000137PMID:23697806
Ensembl transcript structure with UTRs, exons and introns


Exon Start End
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF04130 Pfam IPR007259 Gamma-tubulin complex component protein 192 665 4

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.94 Da
Charge -8.50
Isoelectric point 5.20
Molecular weight 87.24 kDa
Number of residues 759
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
NDduring GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.1during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
0.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
no apparent S. cerevisiae ortholog601
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
duo1DASH complex subunit Duo1 Negative GeneticPMID:22681890
mph1dual specificity protein kinase Mph1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
klp6kinesin-like protein Klp6 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
SPAC1610.02cmitochondrial ribosomal protein subunit L1 (predicted) Positive GeneticPMID:22681890
yaf9YEATS family histone acetyltransferase subunit Yaf9 Negative GeneticPMID:18818364
tpp1trehalose-6-phosphate phosphatase Tpp1 Negative GeneticPMID:22681890
mad2spindle checkpoint protein Mad2 Negative GeneticPMID:22681890
atp15F0-ATPase epsilon subunit (predicted) Negative GeneticPMID:22681890
mga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Negative GeneticPMID:22681890
rpa12DNA-directed RNA polymerase complex I subunit Rpa12 Negative GeneticPMID:22681890
get1GET complex subunit Get1 (predicted) Negative GeneticPMID:18818364
alp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Dosage RescuePMID:12134075
Synthetic Lethality
eso1sister chromatid cohesion protein/DNA polymerase eta Eso1 fusion protein Negative GeneticPMID:23050226
bob1prefoldin subunit 5 (predicted) Negative GeneticPMID:22681890
sre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
ctf18RFC-like complex subunit Ctf18 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
ddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
prp1U4/U6 x U5 tri-snRNP complex subunit Prp1 Positive GeneticPMID:22681890
ask1DASH complex subunit Ask1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
alp4gamma tubulin complex Spc97/GCP2 subunit Alp4 Synthetic LethalityPMID:12134075
Dosage Rescue
dad4DASH complex subunit Dad4 Negative GeneticPMID:22681890
alp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
ash2Ash2-trithorax family protein Negative GeneticPMID:22681890
dad2DASH complex subunit Dad2 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
cph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:18818364
fin1serine/threonine protein kinase, NIMA related Fin1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
git5heterotrimeric G protein beta subunit Git5 Negative GeneticPMID:22681890
msc1multi-copy suppressor of Chk1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
git3G-protein coupled receptor Git3 Negative GeneticPMID:22681890
swc5Swr1 complex subunit Swc5 Negative GeneticPMID:22681890
tom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
bdf2BET family double bromodomain protein Bdf2 Negative GeneticPMID:18818364
dad3DASH complex subunit Dad3 Negative GeneticPMID:22681890
lsg1Lsk1 complex gamma subunit (predicted) Negative GeneticPMID:22681890
swc2Swr1 complex complex subunit Swc2 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
pht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
swr1SNF2 family helicase Swr1 Negative GeneticPMID:22681890
erp2COPII-coated vesicle component Erp2/3/4 (predicted) Negative GeneticPMID:22681890
spc34DASH complex subunit Spc34 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
hmt2sulfide-quinone oxidoreductase Negative GeneticPMID:22681890
ntp1alpha,alpha-trehalase Ntp1 Negative GeneticPMID:22681890
spc19DASH complex subunit Spc19 Negative GeneticPMID:22681890
psh3ER chaperone SHR3 homologue Psh3 Negative GeneticPMID:22681890
arp6actin-like protein Arp6 Negative GeneticPMID:22681890
prw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
SPBC36B7.08cnucleosome assembly protein (predicted) Negative GeneticPMID:22681890
snr13-hydroxyisobutyryl-CoA hydrolase snr1 Positive GeneticPMID:22681890
SPAC3A11.13prefoldin subunit 6 (predicted) Negative GeneticPMID:18818364
pac10prefoldin subunit 3 Pac10 (predicted) Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
SPAP8A3.07cphospho-2-dehydro-3-deoxyheptonate aldolase (predicted) Negative GeneticPMID:22681890
SPAC3H8.02sec14 cytosolic factor family phosphatidylinositol transporter (predicted) Negative GeneticPMID:22681890
dam1DASH complex subunit Dam1 Negative GeneticPMID:22681890
exg3glucan 1,3-beta-glucosidase Exg3 Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
alp6gamma tubulin complex Spc98/GCP3 subunit Alp6 Affinity Capture-MSPMID:15004232
gtb1gamma-tubulin Gtb1 Affinity Capture-WesternPMID:12134075
gfh1gamma tubulin complex subunit Gfh1 Affinity Capture-WesternPMID:17021256
alp4gamma tubulin complex Spc97/GCP2 subunit Alp4 Affinity Capture-WesternPMID:21444751
Affinity Capture-MSPMID:15004232
Affinity Capture-WesternPMID:17021256
mod21gamma tubulin complex subunit Mod21 Affinity Capture-WesternPMID:17021256
External References
Database Identifier Description
NBRP SPCC4G3.19 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC4G3.19 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC4G3.19 BioGRID Interaction Datasets
Expression Viewer SPCC4G3.19 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC4G3.19 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC4G3.19 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC4G3.19 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC4G3.19 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC4G3.19 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC4G3.19 Cell Cycle Data
GEO SPCC4G3.19 GEO profiles
PInt SPCC4G3.19 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC4G3.19 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN28/28B12Orfeome Localization Data
UniProtKB/SwissProtP87244Spindle pole body component alp16
ModBaseP87244Database of comparative protein structure models
STRINGP87244Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587819gamma tubulin complex subunit Alp16
RefSeq mRNANM_001022812972h- gamma tubulin complex subunit Alp16 (alp16), mRNA
European Nucleotide ArchiveBAB79248.1ENA Protein Mapping
European Nucleotide ArchiveCAB09763.1ENA Protein Mapping
UniParcUPI0000125798UniProt Archive

Literature for alp16

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014