nse1 (SPCC550.05)

Gene Standard Namense1 Characterisation Statuspublished
Systematic IDSPCC550.05 Feature Typeprotein coding
Synonyms Name Description
ProductSmc5-6 complex non-SMC subunit 1 Product Size232aa, 27.05 kDa
Genomic Location Chromosome III, 1193982-1196023 (2042nt); CDS:1194197-1194943 (747nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0046872metal ion bindingIEAUniProtKB-KW:KW-0479GO_REF:0000037748
GO:0005515protein bindingIPIsmc5PMID:12966087865
GO:0061630ubiquitin protein ligase activityTASPMID:1533176471
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006281DNA repairIMPPMID:12966087158
GO:0000724double-strand break repair via homologous recombinationIGIrad51PMID:1296608731
GO:0006311meiotic gene conversionIMPPMID:153317648
GO:0016567protein ubiquitinationTASPMID:15331764108
GO:0007131reciprocal meiotic recombinationIMPPMID:1533176438
GO:0048478replication fork protectionIGIbrc1PMID:1533176412
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030915Smc5-Smc6 complexIDAPMID:129660878
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopynse1ΔNullPECO:0000005, PECO:0000137PMID:236978061337
Microscopynot recorded (unrecorded)PMID:12966087

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopynse1ΔNullPECO:0000004, PECO:0000137PMID:23697806640
FYPO:0001122elongated vegetative cellMicroscopynot recorded (unrecorded)PMID:12966087663
FYPO:0001490inviable elongated vegetative cell389
penetrance FYPO_EXT:0000003Microscopynse1ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0001511inviable vegetative cell, abnormal cell shape, normal cell size199
expressivity FYPO_EXT:0000003
penetrance FYPO_EXT:0000001
Microscopynse1ΔNullPECO:0000137, PECO:0000005PMID:23697806
Ensembl transcript structure with UTRs, exons and introns


Exon Start End
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF07574 Pfam IPR011513 Non-structural maintenance of chromosomes element 1 11 169 1
PTHR20973:SF0 HMMPANTHER 8 231 1
PTHR20973 HMMPANTHER IPR011513 Non-structural maintenance of chromosomes element 1 8 231 1

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000173zf-C3HC4 type (RING finger)PMID:15331764Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000173
PBO:0000176ubiquitin-protein ligase E3PMID:15331764Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000176

Protein Properties

Ave. residue weight 116.60 Da
Charge -5.00
Isoelectric point 5.53
Molecular weight 27.05 kDa
Number of residues 232
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1900during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.2during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
0.84during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
gene structure updated PMID:12966087432
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
rec12endonuclease Rec12 Synthetic RescuePMID:15331764
brc1BRCT domain protein Brc1 Synthetic LethalityPMID:15331764
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
rps00140S ribosomal protein S0A (p40) Two-hybridPMID:15331764
SPCC1827.05cnucleolar RNA-binding protein NIFK (predicted) Two-hybridPMID:15331764
smc6Smc5-6 complex SMC subunit Smc6 Affinity Capture-WesternPMID:14701739
Affinity Capture-MSPMID:15601840
smc5Smc5-6 complex SMC subunit Smc5 Affinity Capture-MSPMID:15331764
Affinity Capture-MSPMID:16478984
Reconstituted Complex
Affinity Capture-MSPMID:12966087
Affinity Capture-Western
cbc1nuclear cap-binding complex large subunit (predicted) Two-hybridPMID:15331764
nse3Smc5-6 complex non-SMC subunit Nse3 Two-hybridPMID:21364888
Affinity Capture-Western
Reconstituted ComplexPMID:15601840
nse4Smc5-6 complex non-SMC subunit Nse4 Affinity Capture-MSPMID:16478984
nse5Smc5-6 complex non-SMC subunit Nse5 Affinity Capture-WesternPMID:16478984
Affinity Capture-MS
pgk1phosphoglycerate kinase Pgk1 (predicted) Two-hybridPMID:15331764
nse6Smc5-6 complex non-SMC subunit Nse6 Affinity Capture-WesternPMID:16478984
Affinity Capture-MS
External References
Database Identifier Description
NBRP SPCC550.05 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC550.05 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC550.05 BioGRID Interaction Datasets
Expression Viewer SPCC550.05 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC550.05 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC550.05 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC550.05 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC550.05 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC550.05 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC550.05 Cell Cycle Data
GEO SPCC550.05 GEO profiles
PInt SPCC550.05 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC550.05 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN06/06H04Orfeome Localization Data
UniProtKB/SwissProtQ53EK2Non-structural maintenance of chromosomes element 1
ModBaseQ53EK2Database of comparative protein structure models
STRINGQ53EK2Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588098mitochondrial heat shock protein Hsp10 (predicted)
RefSeq mRNANM_001023089972h- mitochondrial heat shock protein Hsp10 (predicted) (hsp10), mRNA
RefSeq mRNAXM_001713113972h- Smc5-6 complex non-SMC subunit 1 (nse1), mRNA
RefSeq PeptideXP_001713165Smc5-6 complex non-SMC subunit 1
European Nucleotide ArchiveCAI84978.1ENA Protein Mapping
UniParcUPI000050A499UniProt Archive

Literature for nse1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014