cuf2 (SPCC584.02)


Gene Standard Namecuf2 Characterisation Statuspublished
Systematic IDSPCC584.02 Feature Typeprotein coding
Synonyms Name Description
Productmiddle-meiotic transcription factor Cuf2 Product Size177aa, 20.07 kDa
Genomic Location Chromosome III, 1532126-1532753 (628nt); CDS:1532126-1532753 (628nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
copper ion binding15
Annotation ExtensionEvidenceWith/FromReference
DNA binding352
Annotation ExtensionEvidenceWith/FromReference
RNA polymerase II core promoter proximal region sequence-specific DNA binding92
Annotation ExtensionEvidenceWith/FromReference
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding88
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased spore germination frequencycuf269
sensitive to cadmiumcuf2Δ249
sensitive to hydroxyureacuf2Δ594
sensitive to methyl methanesulfonatecuf2Δ331
viable vegetative cell populationcuf2Δ3850

Cell Phenotype

Term NameGenotypesCount
abnormal sporulationcuf2178
abnormal sporulation resulting in formation of azygotic ascus with more or fewer than four sporescuf2Δ4
increased level of middle meiotic gene mRNA during late meiosis1
affecting SPAC1B2.03ccuf2Δ
affecting meu14cuf2Δ
affecting SPBC1348.01cuf2Δ
affecting wtf13cuf2Δ
viable vegetative cell with normal cell morphologycuf2Δ3102
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1532126..1532157, 1532208..1532300, 1532345..1532753
Intron1532158..1532207, 1532301..1532344
mRNA1532126..1532753
CDS1532126..1532157, 1532208..1532300, 1532345..1532753
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00649 Pfam IPR001083 Copper fist DNA-binding domain 2 39 2
SM01090 SMART IPR001083 Copper fist DNA-binding domain 1 40 2
SM00412 SMART IPR001083 Copper fist DNA-binding domain 1 40 2
PS01119 Prosite Patterns IPR001083 Copper fist DNA-binding domain 1 39 2
PS50073 Prosite Profiles IPR001083 Copper fist DNA-binding domain 1 40 2
PTHR28088:SF2 HMMPANTHER 2 61 2
PTHR28088 HMMPANTHER 2 61 2
1co4A00 Gene3D IPR001083 Copper fist DNA-binding domain 1 40 2
0036077 SuperFamily IPR001083 Copper fist DNA-binding domain 1 40 2
PR00617 PRINTS IPR001083 Copper fist DNA-binding domain 27 39 2
PR00617 PRINTS IPR001083 Copper fist DNA-binding domain 1 13 2
PR00617 PRINTS IPR001083 Copper fist DNA-binding domain 14 26 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.39 Da
Charge 5.00
Codon Adaptation Index 0.37
Isoelectric point 8.28
Molecular weight 20.07 kDa
Number of residues 177
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelabsent during GO:0071280RNA protection assay evidencePMID:22558440
absent during GO:0035874RNA protection assay evidencePMID:22558440
absent during GO:0072690RNA protection assay evidencePMID:22558440

Quantitative Gene Expression

View graphical display of gene expression data for cuf2 (SPCC584.02)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.048during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.031during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi only616
conserved in fungi4607
conserved in eukaryotes4517
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPCC584.02 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withmss1mitochondrial GTPase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withgcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
negative genetic interaction withmug154conserved fungal protein Negative GeneticPMID:18818364
negative genetic interaction withpus4tRNA pseudouridine synthase Pus4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withvps3CORVET complex subunit, GTPase regulator Vps3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withgpa2heterotrimeric G protein alpha-2 subunit Gpa2 Negative GeneticPMID:22681890
negative genetic interaction withtpp1trehalose-6-phosphate phosphatase Tpp1 Negative GeneticPMID:22681890
negative genetic interaction withatp10mitochondrial F1-F0 ATPase assembly protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withgma12alpha-1,2-galactosyltransferase Gma12 Negative GeneticPMID:22681890
negative genetic interaction withwhi5cell cycle transcriptional repressor Whi5 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withtrm112protein and tRNA methyltransferase regulatory subunit Trm112 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPCC16A11.03cDUF2009 protein Negative GeneticPMID:22681890
negative genetic interaction withxlf1XRCC4-like nonhomologous end joining factor, Cernunnon Xlf1/Nej1 Negative GeneticPMID:22681890
negative genetic interaction withSPAC323.03cSchizosaccharomyces specific protein Negative GeneticPMID:22681890
negative genetic interaction witharp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:18818364
negative genetic interaction withatp15F0-ATPase epsilon subunit (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcgi121EKC/KEOPS complex subunit Cgi121 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withvph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:18818364
negative genetic interaction withsck2serine/threonine protein kinase Sck2 Negative GeneticPMID:24463365
negative genetic interaction withcox19mitochondrial respiratory chain complex IV assembly protein Cox19 (predicted) Negative GeneticPMID:22681890
negative genetic interaction witherd1Erd1 homolog (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmeu27UPF0300 family protein 5 Negative GeneticPMID:22681890
negative genetic interaction withprp1U4/U6 x U5 tri-snRNP complex subunit Prp1 Negative GeneticPMID:22681890
negative genetic interaction withppr2mitochondrial PPR repeat protein Ppr2 Negative GeneticPMID:22681890
negative genetic interaction withtrx2mitochondrial thioredoxin Trx2 Negative GeneticPMID:22681890
negative genetic interaction withamt1ammonium transmembrane transporter Amt1 Negative GeneticPMID:22681890
negative genetic interaction withtcg1single-stranded telomeric binding protein Tgc1 Negative GeneticPMID:22681890
negative genetic interaction withSPCC63.06human WDR89 family WD repeat protein Negative GeneticPMID:22681890
negative genetic interaction withSPAC227.17cconserved eukaryotic protein, DUF3128, human c22orf39 ortholog Negative GeneticPMID:22681890
positive genetic interaction withdis323'-5'-exoribonuclease activity Dis3L2 Positive GeneticPMID:22681890
positive genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:18818364
positive genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Positive GeneticPMID:22681890
positive genetic interaction withmre11Mre11 nuclease Positive GeneticPMID:18818364
positive genetic interaction withres1MBF transcription factor complex subunit Res1 Positive GeneticPMID:22681890
rescued by overexpression ofmfr1meiotic APC activator Mfr1 Dosage RescuePMID:23628763
rescuesmes1meiotic APC inhibitor Mes1 Phenotypic SuppressionPMID:23628763
External References
Database Identifier Description
NBRP SPCC584.02 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC584.02 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC584.02 BioGRID Interaction Datasets
Expression Viewer SPCC584.02 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC584.02 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC584.02 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC584.02 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC584.02 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC584.02 Transcriptome Viewer (Bähler Lab)
GEO SPCC584.02 GEO profiles
PInt SPCC584.02 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC584.02 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC584.02 Fission yeast phenotypic data & analysis
Cyclebase SPCC584.02.1 Cell Cycle Data
SPD / RIKEN42/42E07Orfeome Localization Data
UniProtKB/SwissProtO94588Copper-binding regulatory protein cuf2
ModBaseO94588Database of comparative protein structure models
STRINGO94588Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588223Cu metalloregulatory transcription factor Cuf2 (predicted)
RefSeq mRNANM_001023213972h- Cu metalloregulatory transcription factor Cuf2 (predicted) (cuf2), mRNA
European Nucleotide ArchiveAJ243834ENA EMBL mapping
European Nucleotide ArchiveCAA21819.1ENA Protein Mapping
UniParcUPI00001286E1UniProt Archive

Literature for cuf2

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016