cuf2 (SPCC584.02)

Gene Standard Namecuf2 Characterisation Statuspublished
Systematic IDSPCC584.02 Feature Typeprotein coding
Synonyms Name Description
Productmiddle-meiotic transcription factor Cuf2 Product Size177aa, 20.07 kDa
Genomic Location Chromosome III, 1532126-1532753 (628nt); CDS:1532126-1532753 (628nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
ion homeostasis103
Annotation ExtensionEvidenceWith/FromReference
regulation of meiosis by regulation of transcription from RNA polymerase II promoter3
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000096sensitive to cadmium246
expressivity FYPO_EXT:0000003Cell growth assaycuf2ΔNullPECO:0000102, PECO:0000005, PECO:0000137PMID:18684775
FYPO:0000088sensitive to hydroxyureaCell growth assaycuf2ΔNullPECO:0000137, PECO:0000005PMID:23173672514
FYPO:0000089sensitive to methyl methanesulfonateCell growth assaycuf2ΔNullPECO:0000137, PECO:0000005PMID:23173672219
FYPO:0002060viable vegetative cell populationMicroscopycuf2ΔNullPMID:204732893759
Microscopycuf2ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000121abnormal sporulationMicroscopycuf2NullPECO:0000127PMID:22558440193
FYPO:0001893abnormal sporulation resulting in formation of azygotic ascus with more or fewer than four sporesMicroscopycuf2ΔNullPMID:225584404
FYPO:0000581decreased spore germinationCell growth assaycuf2NullPECO:0000137PMID:2255844021
FYPO:0001891increased level of middle meiotic gene RNA during late meiosis1
affecting meu14Transcript expression level evidencecuf2ΔNullPECO:0000004PMID:22558440
affecting SPAC1B2.03cTranscript expression level evidencecuf2ΔNullPECO:0000004PMID:22558440
affecting SPBC1348.01Transcript expression level evidencecuf2ΔNullPECO:0000004PMID:22558440
affecting wtf13Transcript expression level evidencecuf2ΔNullPECO:0000004PMID:22558440
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopycuf2ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
FYPO affected by mutation in mei4 meiotic forkhead transcription factor Mei4 PMID:22558440
FYPO affected by mutation in ran1 serine/threonine protein kinase Ran1 PMID:22558440
Ensembl transcript structure with UTRs, exons and introns


Exon Start End
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00649 Pfam IPR001083 Copper fist DNA-binding 1 40 2
SM01090 SMART 1 40 2
SM00412 SMART IPR001083 Copper fist DNA-binding 1 40 2
PS01119 Prosite Patterns IPR001083 Copper fist DNA-binding 1 39 2
PS50073 Prosite Profiles IPR001083 Copper fist DNA-binding 1 40 2
1co4A00 Gene3D IPR001083 Copper fist DNA-binding 1 40 2
0036077 SuperFamily IPR001083 Copper fist DNA-binding 1 40 2
PR00617 PRINTS IPR001083 Copper fist DNA-binding 1 13 2
PR00617 PRINTS IPR001083 Copper fist DNA-binding 14 26 2
PR00617 PRINTS IPR001083 Copper fist DNA-binding 27 39 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.39 Da
Charge 5.00
Isoelectric point 8.28
Molecular weight 20.07 kDa
Number of residues 177
Gene Expression

Qualitative Gene Expression

RNA levelabsent during GO:0035874RNA protection assay evidencePMID:22558440
absent during GO:0072690RNA protection assay evidencePMID:22558440
absent during GO:0071280RNA protection assay evidencePMID:22558440

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.048during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
0.031during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
conserved in fungi only623
conserved in fungi4600
conserved in eukaryotes4514

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
SPAC323.03cSchizosaccharomyces specific protein Negative GeneticPMID:22681890
gpa2heterotrimeric G protein alpha-2 subunit Gpa2 Negative GeneticPMID:22681890
prw1Clr6 histone deacetylase complex subunit Prw1 Positive GeneticPMID:22681890
atp15F0-ATPase epsilon subunit (predicted) Negative GeneticPMID:22681890
SPCC16A11.03cDUF2009 protein Negative GeneticPMID:22681890
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:18818364
cgi121EKC/KEOPS complex subunit Cgi121 (predicted) Negative GeneticPMID:22681890
tcg1single-stranded telomeric binding protein Tgc1 Negative GeneticPMID:22681890
SPCC1672.04cmitochondrial copper chaperone (predicted) Negative GeneticPMID:22681890
SPCC63.06human WDR89 family WD repeat protein Negative GeneticPMID:22681890
erd1Erd1 homolog (predicted) Negative GeneticPMID:22681890
vps3CORVET complex subunit, GTPase regulator Vps3 (predicted) Negative GeneticPMID:22681890
prp1U4/U6 x U5 tri-snRNP complex subunit Prp1 Negative GeneticPMID:22681890
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:18818364
trm112protein and tRNA methyltransferase regulatory subunit Trm112 (predicted) Negative GeneticPMID:22681890
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
mss1mitochondrial GTPase (predicted) Negative GeneticPMID:22681890
mug154conserved fungal protein Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
sck2serine/threonine protein kinase Sck2 Negative GeneticPMID:24463365
dis323'-5'-exoribonuclease activity Dis3L2 Positive GeneticPMID:22681890
whi5cell cycle transcriptional repressor Whi5 (predicted) Negative GeneticPMID:22681890
arp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:18818364
gma12alpha-1,2-galactosyltransferase Gma12 Negative GeneticPMID:22681890
SPBC11C11.10tRNA pseudouridine synthase (predicted) Negative GeneticPMID:22681890
ppr2mitochondrial PPR repeat protein Ppr2 Negative GeneticPMID:22681890
mre11Mre11 nuclease Positive GeneticPMID:18818364
meu27UPF0300 family protein 5 Negative GeneticPMID:22681890
res1MBF transcription factor complex subunit Res1 Positive GeneticPMID:22681890
mfr1fizzy-related protein Mfr1 Dosage RescuePMID:23628763
SPAC227.17cconserved protein Negative GeneticPMID:22681890
xlf1xrcc4 like factor, cernunnon Negative GeneticPMID:22681890
tpp1trehalose-6-phosphate phosphatase Tpp1 Negative GeneticPMID:22681890
trx2mitochondrial thioredoxin Trx2 Negative GeneticPMID:22681890
amt1ammonium transporter Amt1 Negative GeneticPMID:22681890
atp10F1-F0 ATPase assembly protein (predicted) Negative GeneticPMID:22681890
mes1meiosis II protein Mes1 Phenotypic SuppressionPMID:23628763
External References
Database Identifier Description
NBRP SPCC584.02 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC584.02 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC584.02 BioGRID Interaction Datasets
Expression Viewer SPCC584.02 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC584.02 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC584.02 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC584.02 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC584.02 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC584.02 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC584.02 Cell Cycle Data
GEO SPCC584.02 GEO profiles
PInt SPCC584.02 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC584.02 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC584.02 Fission yeast phenotypic data & analysis
SPD / RIKEN42/42E07Orfeome Localization Data
UniProtKB/SwissProtO94588Copper-binding regulatory protein cuf2
ModBaseO94588Database of comparative protein structure models
STRINGO94588Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588223Cu metalloregulatory transcription factor Cuf2 (predicted)
RefSeq mRNANM_001023213972h- Cu metalloregulatory transcription factor Cuf2 (predicted) (cuf2), mRNA
European Nucleotide ArchiveAJ243834ENA EMBL mapping
European Nucleotide ArchiveCAA21819.1ENA Protein Mapping
UniParcUPI00001286E1UniProt Archive

Literature for cuf2

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014