taf5 (SPCC5E4.03c)


Gene Standard Nametaf5 Characterisation Statuspublished
Systematic IDSPCC5E4.03c Feature Typeprotein coding
Synonymstaf72 Name Description
ProductSAGA complex subunit/TATA-binding protein associated factor/transcription factor TFIID complex subunit Taf5 Product Size643aa, 72.38 kDa
Genomic Location Chromosome III, 642561-640083 (2479nt); CDS:642444-640145 (2300nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005515protein binding865
requires Pfam:PF00400IPItaf6PMID:11972332
requires Pfam:PF04494IPItaf6PMID:11972332
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006338chromatin remodelingICGO:0000124GO_REF:0000001151
GO:0016573histone acetylationICGO:0000124GO_REF:000000139
GO:0006355regulation of transcription, DNA-templatedIEAIPR007582GO_REF:0000002377
GO:0006367transcription initiation from RNA polymerase II promoterICGO:0005669GO_REF:000000134
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000124SAGA complexIDAPMID:1197233219
IDAPMID:19056896
GO:0005829cytosolIDAPMID:168233722319
GO:0005634nucleusIDAPMID:168233722737
GO:0005669transcription factor TFIID complexIDAPMID:1197233216
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopytaf5ΔNullPECO:0000005, PECO:0000137PMID:236978061337
Microscopytaf5ΔNullPMID:20473289
Microscopynot recorded (unrecorded)PMID:11279037

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002148inviable after spore germination, multiple cell divisions, abnormal morphology187
penetrance FYPO_EXT:0000001Microscopytaf5ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0000049inviable cellCell growth assaytaf5ΔNullPMID:216429551389
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1642561642138
2641924640831
3640774640606
4640556640292
5640241640083
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00400 Pfam IPR001680 WD40 repeat 375 407 104
PF00400 Pfam IPR001680 WD40 repeat 497 533 104
PF00400 Pfam IPR001680 WD40 repeat 540 575 104
PF00400 Pfam IPR001680 WD40 repeat 414 449 104
PF00400 Pfam IPR001680 WD40 repeat 455 491 104
PF00400 Pfam IPR001680 WD40 repeat 315 352 104
PF04494 Pfam IPR007582 TFIID subunit, WD40-associated region 45 184 2
PF08513 Pfam IPR013720 LisH dimerisation motif, subgroup 10 36 8
SM00320 SMART IPR001680 WD40 repeat 494 533 112
SM00320 SMART IPR001680 WD40 repeat 313 352 112
SM00320 SMART IPR001680 WD40 repeat 536 575 112
SM00320 SMART IPR001680 WD40 repeat 452 491 112
SM00320 SMART IPR001680 WD40 repeat 410 449 112
SM00320 SMART IPR001680 WD40 repeat 369 407 112
SM00667 SMART IPR006594 LisH dimerisation motif 8 40 10
PS50082 Prosite Profiles IPR001680 WD40 repeat 501 542 100
PS50082 Prosite Profiles IPR001680 WD40 repeat 543 576 100
PS50082 Prosite Profiles IPR001680 WD40 repeat 459 500 100
PS50082 Prosite Profiles IPR001680 WD40 repeat 375 416 100
PS50082 Prosite Profiles IPR001680 WD40 repeat 417 449 100
PS50294 Prosite Profiles IPR017986 WD40-repeat-containing domain 320 584 104
PS50082 Prosite Profiles IPR001680 WD40 repeat 320 361 100
PS50896 Prosite Profiles IPR006594 LisH dimerisation motif 8 40 11
PS00678 Prosite Patterns IPR019775 WD40 repeat, conserved site 562 576 65
PS00678 Prosite Patterns IPR019775 WD40 repeat, conserved site 478 492 65
PTHR19879:SF0 HMMPANTHER 50 639 2
PTHR19879 HMMPANTHER 50 639 2
2.130.10.10 Gene3D IPR015943 WD40/YVTN repeat-like-containing domain 313 601 145
SSF50978 SuperFamily IPR017986 WD40-repeat-containing domain 312 617 135
SSF160897 SuperFamily 45 188 2
PR00320 PRINTS IPR020472 G-protein beta WD-40 repeat 478 492 43
PR00320 PRINTS IPR020472 G-protein beta WD-40 repeat 562 576 43
PR00320 PRINTS IPR020472 G-protein beta WD-40 repeat 394 408 43

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000190WD repeat proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000190

Protein Properties

Ave. residue weight 112.57 Da
Charge 0.00
Isoelectric point 6.51
Molecular weight 72.38 kDa
Number of residues 643
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS256PMID:247631071670
present during cellular response to thiabendazoleIDAS256PMID:18257517
present during mitotic M phaseS256PMID:21712547
MOD:00696phosphorylated residueNASPMID:182575171922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4392during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
4506during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
4792during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
4392during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
4263.44during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
4912during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2382.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.7during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.92during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
ubc11ubiquitin conjugating enzyme E2-C, Ubc11 Dosage RescuePMID:11279037
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
get1GET complex subunit Get1 (predicted) Negative GeneticPMID:22681890
vps38phophatidylinositol 3-kinase complex subunit Vps38 Negative GeneticPMID:22681890
cut9anaphase-promoting complex subunit Cut9 Dosage RescuePMID:11279037
gcd10tRNA (m1A) methyltransferase Gcd10 (predicted) Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
tra1SAGA complex phosphatidylinositol pseudokinase Tra1 Affinity Capture-MSPMID:21642955
taf6histone H4-like TAF Taf6, SAGA complex subunit Reconstituted ComplexPMID:11972332
Two-hybrid
Affinity Capture-Western
hfi1SAGA complex subunit Hfi1 Affinity Capture-MSPMID:19056896
taf111transcription factor TFIID complex subunit Taf111 Affinity Capture-WesternPMID:11972332
Affinity Capture-WesternPMID:11279037
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
tbp1TATA-binding protein (TBP) Affinity Capture-WesternPMID:11972332
Affinity Capture-WesternPMID:11279037
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
ada1adenosine deaminase Ada1 Affinity Capture-MSPMID:21642955
tfg3transcription factor TFIIF complex subunit Tfg3 Affinity Capture-WesternPMID:15616156
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
ptr6transcription factor TFIID complex subunit Taf7 Affinity Capture-WesternPMID:11972332
Affinity Capture-WesternPMID:11279037
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Affinity Capture-WesternPMID:11279037
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
spt7SAGA complex bromodomain subunit Spt7 Affinity Capture-MSPMID:19056896
External References
Database Identifier Description
NBRP SPCC5E4.03c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC5E4.03c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC5E4.03c BioGRID Interaction Datasets
Expression Viewer SPCC5E4.03c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC5E4.03c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC5E4.03c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC5E4.03c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC5E4.03c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC5E4.03c Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC5E4.03c Cell Cycle Data
GEO SPCC5E4.03c GEO profiles
PInt SPCC5E4.03c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC5E4.03c Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN38/38B09Orfeome Localization Data
UniProtKB/SwissProtO13282Transcription initiation factor TFIID subunit 5
ModBaseO13282Database of comparative protein structure models
STRINGO13282Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587902transcription initiation factor TFIID subunit 5
RefSeq mRNANM_001022894972h- transcription initiation factor TFIID subunit 5 (taf5), mRNA
European Nucleotide ArchiveBAA22162.1ENA Protein Mapping
European Nucleotide ArchiveCAA21958.1ENA Protein Mapping
UniParcUPI00001367E4UniProt Archive

Literature for taf5

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014