SPCC613.03


Gene Standard NameUnassigned Characterisation Statusconserved_unknown
Systematic IDSPCC613.03 Feature Typeprotein coding
Synonyms Name Description
Productconserved fungal protein Product Size189aa, 22.48 kDa
Genomic Location Chromosome III, 85128-88460 (3333nt); CDS:85363-85932 (570nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
calcium ion binding38
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4206
Annotation ExtensionEvidenceWith/FromReference
endoplasmic reticulum596
Annotation ExtensionEvidenceWith/FromReference
endoplasmic reticulum lumen13
Annotation ExtensionEvidenceWith/FromReference
Golgi apparatus357
Annotation ExtensionEvidenceWith/FromReference
Golgi lumen2
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
sensitive to hydroxyureaSPCC613.03ΔNull525
sensitive to methyl methanesulfonateSPCC613.03ΔNull227
sensitive to tacrolimusSPCC613.03ΔNull81
slow vegetative cell population growthSPCC613.03ΔNull318
viable vegetative cell populationSPCC613.03ΔNull3783

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologySPCC613.03ΔNull3092
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
18512888460

UTRs

Region Coordinates Reference
five_prime_UTR85128..85362PMID:21511999
three_prime_UTR85933..88460PMID:21511999
mRNA85128..88460
exon85363..85932
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PS00018 Prosite Patterns IPR018247 EF-Hand 1, calcium-binding site 87 99 12
PS50222 Prosite Profiles IPR002048 EF-hand domain 74 109 20
PTHR19237 HMMPANTHER 20 186 1
1.10.238.10 Gene3D IPR011992 EF-hand domain pair 26 105 20
SSF47473 SuperFamily 24 104 21
SignalP-noTM signalp 1 20 212

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000276EF handTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000276

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0000418signal_peptide279

Protein Properties

Ave. residue weight 118.92 Da
Charge -6.00
Isoelectric point 5.68
Molecular weight 22.48 kDa
Number of residues 189
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
14734during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
15566during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
13528during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
14820during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
14169during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
6858.31during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
4972.23during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.57during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only619
conserved in fungi4604
conserved in eukaryotes4516
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withchk1Chk1 protein kinase Two-hybridPMID:17502373
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withace2transcription factor Ace2 Positive GeneticPMID:22681890
negative genetic interaction withask1DASH complex subunit Ask1 Negative GeneticPMID:22681890
negative genetic interaction withatg6beclin family protein, involved in autophagy Negative GeneticPMID:22681890
negative genetic interaction withcph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
negative genetic interaction withdad2DASH complex subunit Dad2 Negative GeneticPMID:22681890
positive genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Positive GeneticPMID:22681890
negative genetic interaction withmyp2myosin II heavy chain Myo3 Negative GeneticPMID:22681890
negative genetic interaction withpar1protein phosphatase regulatory subunit Par1 Negative GeneticPMID:22681890
negative genetic interaction withpht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:22681890
positive genetic interaction withpof9F-box protein Pof9 Positive GeneticPMID:22681890
negative genetic interaction withpst2Clr6 histone deacetylase complex subunit Pst2 Negative GeneticPMID:22681890
negative genetic interaction withpsy2protein phosphatase PP4 complex subunit Psy2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
positive genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withsts5RNB-like protein Negative GeneticPMID:22681890
negative genetic interaction withswc5Swr1 complex subunit Swc5 Negative GeneticPMID:22681890
negative genetic interaction withxap5xap-5-like protein Negative GeneticPMID:24957674
External References
Database Identifier Description
NBRP SPCC613.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC613.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC613.03 BioGRID Interaction Datasets
Expression Viewer SPCC613.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC613.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC613.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC613.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC613.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC613.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC613.03 Cell Cycle Data
GEO SPCC613.03 GEO profiles
PInt SPCC613.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC613.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC613.03 Fission yeast phenotypic data & analysis
SPD / RIKEN06/06A08Orfeome Localization Data
UniProtKB/SwissProtO74903Uncharacterized calcium-binding protein C613.03
ModBaseO74903Database of comparative protein structure models
STRINGO74903Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587691conserved fungal protein
RefSeq mRNANM_001022686972h- conserved fungal protein (SPCC613.03), mRNA
European Nucleotide ArchiveCAA21055.1ENA Protein Mapping
UniParcUPI000006AF6CUniProt Archive

Literature for SPCC613.03

Search: Europe PMC or PubMed

Release Version: PomBase:25_49 - 02 Feb 2015