rgf1 (SPCC645.07)

Gene Standard Namergf1 ChromosomeIII
Systematic IDSPCC645.07 Gene Start1245211
Synonyms Gene End1249470
ProductRhoGEF for Rho1, Rgf1 Gene Length4260
Feature Typeprotein coding CDS Start1245263
Name Description CDS End1249313
Characterisation Statuspublished CDS Length4051

Ensembl Gene Location
  1. rgf3
  2. SPNCRNA.1181
  3. rgf1
  4. snd1
  5. SPCTRNASER.10
  6. mrpl37
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000190abnormal actin cortical patch localizationnot recorded (overexpression)unknownPMID:16324155
FYPO:0002060viable vegetative cell populationno_name (unrecorded)unrecordedPMID:16291723
Microscopyrgf1delta (deletion)deletionPMID:20473289
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0005089Rho guanyl-nucleotide exchange factor activityIGIrga1PMID:16421249
has_direct_input rho1IGIrho1PMID:16324155
GO:0005515protein bindingIPIrho1PMID:16324155
GO:0005543phospholipid bindingIEAIPR001849GO_REF:0000002
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0008360regulation of cell shapeIGIrga1PMID:16421249
GO:0009272fungal-type cell wall biogenesisIGIbgs1PMID:16324155
same_pathwayIGIrho1PMID:16421249
GO:0032319regulation of Rho GTPase activityIGIrho1PMID:16324155
GO:0035025positive regulation of Rho protein signal transduction
same_pathwayIGIrho1PMID:16421249
GO:0051518positive regulation of bipolar cell growthIMPPMID:16421249
GO:0051666actin cortical patch localizationIMPPMID:16324155
GO:0090334regulation of cell wall (1->3)-beta-D-glucan biosynthetic processIGIbgs4PMID:16421249
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0000935barrier septum
exists_during mitotic cell cycleIDAPMID:16324155
GO:0005634nucleusIDAPMID:16823372
GO:0030428cell septumIDAPMID:16291723
GO:0051285cell cortex of cell tip
exists_during interphase of mitotic cell cycleIDAPMID:16324155
GO:0051286cell tipIDAPMID:16291723
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
112452111247944
212479911249470

References

Region Start End Reference
three_prime_UTR12493141249470PMID:21511999
three_prime_UTR12493141249470PMID:21511999
five_prime_UTR12452111245262PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF00780 IPR001180 Pfam Citron-like 1004 1289 5
PF00610 IPR000591 Pfam DEP domain 429 497 5
PF00621 IPR000219 Pfam Dbl homology (DH) domain 625 806 8
SM00036 IPR001180 SMART Citron-like 994 1305 2
SM00233 IPR001849 SMART Pleckstrin homology domain 844 975 24
SM00325 IPR000219 SMART Dbl homology (DH) domain 625 807 7
SM00049 IPR000591 SMART DEP domain 424 499 5
PS50010 IPR000219 Prosite Profiles Dbl homology (DH) domain 621 808 8
PS50003 IPR001849 Prosite Profiles Pleckstrin homology domain 843 973 22
PS50219 IPR001180 Prosite Profiles Citron-like 995 1293 5
G3DSA:1.20.900.10 IPR000219 Gene3D 600 822 8
G3DSA:1.10.10.10 IPR011991 Gene3D 439 528 43
G3DSA:2.30.29.30 IPR011993 Gene3D 823 977 35
SSF48065 IPR000219 SuperFamily 604 825 8
SSF50729 SuperFamily 834 980 36
SSF46785 SuperFamily 428 503 49
PTHR12673 hmmpanther 35 1021 3
PTHR12673:SF16 hmmpanther 35 1021 1

View domain organization at Pfam

Protein Modifications (PSI-MOD)

Term IDTerm NameEvidenceResidueReference
MOD:00696phosphorylated residuePMID:19547744

Manually Curated Family or Sub-families

Term IDTerm NameReferenceAll Genes
PBO:0000251pleckstrin homology domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000251

Protein Properties

Ave. residue weight 112.53 Da
Charge 44.00
Isoelectric point 9.33
Molecular weight 150.11 kDa
Number of residues 1334
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127		population_widePMID:23101633
experimental evidencePECO:0000201,
PECO:0000005		single_cell4300PMID:16224022
experimental evidencePECO:0000014,
PECO:0000005		population_wide1662.35PMID:23101633
RNA levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide1.2PMID:23101633
experimental evidencePECO:0000014,
PECO:0000005		population_wide4PMID:23101633
Disease Association
DescriptionQualifierReference
cancer
Genome Organisation
DescriptionQualifierReference
divergently orientated to functionally related gene
Species Distribution
DescriptionQualifierReference
conserved in fungi
conserved in eukaryotes
conserved in metazoa
conserved in vertebrates
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
rga1Rho-type GTPase activating protein Rga1 Synthetic RescuePMID:16421249
Synthetic RescuePMID:16324155
rho1Rho family GTPase Rho1 Dosage RescuePMID:16421249
rgf3RhoGEF Rgf3 Dosage RescuePMID:20739711
Dosage RescuePMID:16324155
art1arrestin family protein Dosage RescuePMID:20739711
rgf2RhoGEF Rgf2 Dosage RescuePMID:16324155
bgs41,3-beta-glucan synthase subunit Bgs4 Dosage RescuePMID:16421249
pck1protein kinase C (PKC)-like Pck1 Dosage RescuePMID:16421249
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
rho1Rho family GTPase Rho1 Affinity Capture-WesternPMID:14637153
Affinity Capture-WesternPMID:16421249
Two-hybridPMID:16324155
cdc42Rho family GTPase Cdc42 Affinity Capture-WesternPMID:14637153
rad2414-3-3 protein Rad24 Affinity Capture-MSPMID:23394829
rho5Rho family GTPase Rho5 Affinity Capture-WesternPMID:14637153
myo1myosin type I Affinity Capture-RNAPMID:19805578
its31-phosphatidylinositol-4-phosphate 5-kinase Its3 Co-localizationPMID:15923187
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External References
Database Identifier Description
NBRP SPCC645.07 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC645.07 Retrieval of eukaryotic orthologs
BioGrid SPCC645.07 BioGRID Interaction Datasets
Bähler Lab SPCC645.07 Cell Cycle Expression Profile
Bähler Lab SPCC645.07 Meiosis/Sporulation Expression Profies
Bähler Lab SPCC645.07 Pheromone response/mating expression profiles
Bähler Lab SPCC645.07 Environmental stress expression profiles
Bähler Lab SPCC645.07 Bähler Lab Transcriptome Viewer
Cyclebase SPCC645.07 Cell Cycle Data
PInt SPCC645.07 Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPCC645.07 Entrez Gene
WikiGene2539580RhoGEF for Rho1, Rgf1
Uniprot_genenameRGF1
EntrezGene2539580RhoGEF for Rho1, Rgf1
UniProtKB/SwissProtQ9Y7U6Rho1 guanine nucleotide exchange factor 1
ModBaseQ9Y7U6Database of comparative protein structure models
Pfam Protein DomainsQ9Y7U6Pfam Domain Arrangement
RefSeq PeptideNP_588116RhoGEF for Rho1, Rgf1
RefSeq mRNANM_001023106972h- RhoGEF for Rho1, Rgf1 (rgf1), mRNA
European Nucleotide ArchiveCAB39903ENA Protein Mapping
SPD / RIKEN30/30E12Orfeome Localization Data
UniParcUPI000006976AUniProt Archive

Literature for rgf1
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013