ctt1 (SPCC757.07c)


Gene Standard Namectt1 Characterisation Statuspublished
Systematic IDSPCC757.07c Feature Typeprotein coding
Synonymscta1 Name Description
Productcatalase Product Size512aa, 58.28 kDa
Genomic Location Chromosome III, 58865-56654 (2212nt); CDS:58465-56927 (1539nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
catalase activity1
Annotation ExtensionEvidenceWith/FromReference
heme binding10
Annotation ExtensionEvidenceWith/FromReference
metal ion binding749
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

detoxification


Term NameCount
cellular detoxification45
Annotation ExtensionEvidenceWith/FromReference
cellular response to oxidative stress46
Annotation ExtensionEvidenceWith/FromReference
hydrogen peroxide catabolic process6
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
mitochondrial matrix153
Annotation ExtensionEvidenceWith/FromReference
peroxisomal matrix2
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
normal anaerobic cell population growthctt1Δ8
normal growth on glucose carbon sourcectt1Δ287
normal vegetative cell population growthctt1Δ851
normal viability in stationary phasectt1+29
resistance to hydrogen peroxidectt1+75
sensitive to hydrogen peroxidectt1Δ154
viable vegetative cell populationctt1Δ3844

Cell Phenotype

Term NameGenotypesCount
abnormal hydrogen peroxide catabolismctt1Δ1
abnormal nucleosome positioningctt1.G4BS (ATGACGT-435CGGAAGACTCTCCTCCG)11
abolished catalase activityctt1Δ1
normal cellular reactive oxygen species level during vegetative growthctt1Δ15
viable vegetative cell, abnormal cell shape, normal cell sizectt1Δ215

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
sensitive to hydrogen peroxideecl3+, ctt1Δ
ecl1+, ctt1Δ
ecl2+, ctt1Δ

Cell Phenotype

Term NameGenotypes
normal nucleosome positioning at stress response genesctt1.G4BS (ATGACGT-435CGGAAGACTCTCCTCCG), G4-Atf1 (Atf1 amino acids 1-395 fused to budding yeast Gal4p DNA binding domain)
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in ada2 SAGA complex subunit Ada2
FYPO affected by mutation in ash2 Ash2-trithorax family protein
FYPO affected by mutation in atf1 transcription factor, Atf-CREB family Atf1
FYPO affected by mutation in csx1 RNA-binding protein Csx1
FYPO affected by mutation in dps1 decaprenyl diphosphate synthase subunit Dps1
FYPO affected by mutation in ecl1 extender of the chronological lifespan protein Ecl1
FYPO affected by mutation in ecl2 extender of the chronological lifespan protein Ecl2
FYPO affected by mutation in ecl3 extender of the chronological lifespan protein Ecl3
FYPO affected by mutation in elp3 elongator complex subunit Elp3 (predicted)
FYPO affected by mutation in elp6 elongator complex subunit Elp6 (predicted)
FYPO affected by mutation in gcn5 SAGA complex histone acetyltransferase catalytic subunit Gcn5
FYPO affected by mutation in leo1 RNA polymerase II associated Paf1 complex (predicted)
FYPO affected by mutation in mcs4 response regulator Mcs4
FYPO affected by mutation in mtq2 protein methyltransferase Mtq2 (predicted)
FYPO affected by mutation in ngg1 SAGA complex subunit Ngg1
FYPO affected by mutation in paf1 RNA polymerase II associated Paf1 complex (predicted)
FYPO affected by mutation in pap1 transcription factor Pap1/Caf3
FYPO affected by mutation in pcr1 transcription factor Pcr1
FYPO affected by mutation in prf1 RNA polymerase II associated Paf1 complex (predicted)
FYPO affected by mutation in prr1 transcription factor Prr1
FYPO affected by mutation in pyp1 tyrosine phosphatase Pyp1
FYPO affected by mutation in set1 histone lysine methyltransferase Set1
FYPO affected by mutation in shg1 Set1C complex subunit Shg1
FYPO affected by mutation in spf1 Set1C PHD Finger protein Spf1
FYPO affected by mutation in sty1 MAP kinase Sty1
FYPO affected by mutation in swd1 Set1C complex subunit Swd1
FYPO affected by mutation in swd3 WD repeat protein Swd3
FYPO affected by mutation in trm112 protein and tRNA methyltransferase regulatory subunit Trm112 (predicted)
FYPO affected by mutation in trm9 tRNA (uridine) methyltransferase Trm9 (predicted)
FYPO affected by mutation in trx1 cytosolic thioredoxin Trx1
FYPO affected by mutation in upf1 ATP-dependent RNA helicase Upf1
FYPO affected by mutation in win1 MAP kinase kinase kinase Win1
FYPO affected by mutation in wis1 MAP kinase kinase Wis1
FYPO affected by mutation in wis4 MAP kinase kinase kinase Wis4
GO regulated by atf1 transcription factor, Atf-CREB family Atf1
GO regulated by pap1 transcription factor Pap1/Caf3
GO regulated by sty1 MAP kinase Sty1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons58865..56654
mRNA58865..56654
5' UTR58865..58466PMID:21511999
CDS58465..56927
3' UTR56926..56654PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF06628 Pfam IPR010582 Catalase immune-responsive domain 423 484 1
PF00199 Pfam IPR011614 Catalase core domain 13 392 1
SM01060 SMART IPR011614 Catalase core domain 13 397 1
PS51402 Prosite Profiles IPR018028 Catalase, mono-functional, haem-containing 9 491 1
PS00438 Prosite Patterns IPR024708 Catalase active site 49 65 1
PS00437 Prosite Patterns IPR002226 Catalase haem-binding site 340 348 1
PTHR11465 HMMPANTHER IPR018028 Catalase, mono-functional, haem-containing 10 488 1
2.40.180.10 Gene3D IPR011614 Catalase core domain 3 364 1
SSF56634 SuperFamily IPR020835 Catalase-like domain 8 497 1
PIRSF038928 PIRSF IPR024711 Catalase, mono-functional, haem-containing, clades 1 and 3 1 491 1
PR00067 PRINTS IPR018028 Catalase, mono-functional, haem-containing 89 107 1
PR00067 PRINTS IPR018028 Catalase, mono-functional, haem-containing 129 147 1
PR00067 PRINTS IPR018028 Catalase, mono-functional, haem-containing 327 353 1
PR00067 PRINTS IPR018028 Catalase, mono-functional, haem-containing 110 127 1
PR00067 PRINTS IPR018028 Catalase, mono-functional, haem-containing 295 322 1
PR00067 PRINTS IPR018028 Catalase, mono-functional, haem-containing 26 49 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.83 Da
Charge 3.50
Codon Adaptation Index 0.63
Isoelectric point 6.88
Molecular weight 58.28 kDa
Number of residues 512
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS512 1677
Annotation ExtensionEvidenceResidueReference
experimental evidence S512 PMID:24763107
phosphorylated residue 1932
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
ubiquitinylated lysineK365 512
Annotation ExtensionEvidenceResidueReference
IDA PMID:26412298
mass spectrometry evidence K365 PMID:26412298
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0036245Northern assay evidencePMID:8537298
increased during GO:0070301Northern assay evidencePMID:18758731
increased during GO:0070301Northern assay evidencePMID:23874237
increased during GO:0034605expression microarray evidencePMID:12529438
present during GO:0072690Northern assay evidencePMID:10348908
absent during GO:0072690Northern assay evidencePMID:9585505
increased during GO:0071470expression microarray evidencePMID:12529438
increased during GO:0070301Northern assay evidencePMID:9450957
increased during GO:0071472Northern assay evidencePMID:12221110
increased during GO:0072709Northern assay evidencePMID:8824588
increased during GO:0070301Northern assay evidencePMID:24118096
present during GO:0072690Northern assay evidencePMID:9154834
increased during GO:0071471Northern assay evidencePMID:8537298
increased during GO:0034644Northern assay evidencePMID:9154834
unchanged during GO:0070417Northern assay evidencePMID:8537298
present during GO:0072690Northern assay evidencePMID:23874237
unchanged during GO:0071493Northern assay evidencePMID:8537298
unchanged during GO:0071287Northern assay evidencePMID:8537298
increased during GO:0071494Northern assay evidencePMID:8537298
increased during GO:0070301Northern assay evidencePMID:8537298
increased in presence of cadmium sulfate expression microarray evidencePMID:12529438
increased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
increased during GO:0070301Northern assay evidencePMID:9585505
unchanged during GO:0071276Northern assay evidencePMID:8537298
increased during GO:0034605Northern assay evidencePMID:12221110
increased during GO:0070301Northern assay evidencePMID:12221110
increased during GO:0070301Northern assay evidencePMID:10348908
increased during GO:0071475Northern assay evidencePMID:8537298
increased during GO:0034599expression microarray evidencePMID:12529438

Quantitative Gene Expression

View graphical display of gene expression data for ctt1 (SPCC757.07c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
47621during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
48371during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
49657during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
44065during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
45444during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
7296.16during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
28579.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
metabolic disorders143
peroxisomal metabolism disorders12
acatalasemia1
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in bacteria1002
conserved in metazoa3424
conserved in vertebrates3399
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPCC757.07c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
overexpression rescuestpx1thioredoxin peroxidase Tpx1 Dosage RescuePMID:24521463
overexpression rescuespap1transcription factor Pap1/Caf3 Dosage RescuePMID:24521463
synthetic lethal withtpx1thioredoxin peroxidase Tpx1 Synthetic LethalityPMID:24521463
synthetic lethal withtrr1thioredoxin reductase Trr1 Synthetic LethalityPMID:24521463
rescuesecl3extender of the chronological lifespan protein Ecl3 Phenotypic SuppressionPMID:22212525
rescuesecl1extender of the chronological lifespan protein Ecl1 Phenotypic SuppressionPMID:22212525
rescuesecl2extender of the chronological lifespan protein Ecl2 Phenotypic SuppressionPMID:22212525
enhances phenotype oftpx1thioredoxin peroxidase Tpx1 Phenotypic EnhancementPMID:24521463
synthetic growth defect withtpx1thioredoxin peroxidase Tpx1 Synthetic Growth DefectPMID:24521463
rescued by overexpression ofgpx1glutathione peroxidase Gpx1 Dosage RescuePMID:10455235
External References
Database Identifier Description
NBRP SPCC757.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC757.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC757.07c BioGRID Interaction Datasets
Expression Viewer SPCC757.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC757.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC757.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC757.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC757.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC757.07c Transcriptome Viewer (Bähler Lab)
GEO SPCC757.07c GEO profiles
PInt SPCC757.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC757.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC757.07c Fission yeast phenotypic data & analysis
Cyclebase SPCC757.07c.1 Cell Cycle Data
IntEnz1.11.1.6Integrated relational Enzyme database
Rhea1.11.1.6Annotated reactions database
SPD / RIKEN24/24H11Orfeome Localization Data
UniProtKB/SwissProtP55306Catalase
ModBaseP55306Database of comparative protein structure models
STRINGP55306Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587682catalase
RefSeq mRNANM_001022677972h- catalase (ctt1), mRNA
European Nucleotide ArchiveD89126ENA EMBL mapping
European Nucleotide ArchiveBAA09526.1ENA Protein Mapping
European Nucleotide ArchiveCAA21232.1ENA Protein Mapping
KEGG00380+1.11.1.6Tryptophan metabolism
KEGG00630+1.11.1.6Glyoxylate and dicarboxylate metabolism
UniParcUPI000012707FUniProt Archive

Literature for ctt1

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016