bit61 (SPCC777.08c)


Gene Standard Namebit61 Characterisation Statuspublished
Systematic IDSPCC777.08c Feature Typeprotein coding
SynonymsSPCC777.08 Name Description
ProductTORC2 subunit Bit61 Product Size422aa, 46.86 kDa
Genomic Location Chromosome III, 1612779-1611047 (1733nt); CDS:1612564-1611296 (1269nt)

Ensembl Gene Location
GO Biological Process

GO Slim Terms

signaling


Term NameCount
TOR signaling20
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2316
Annotation ExtensionEvidenceWith/FromReference
nucleus2697
Annotation ExtensionEvidenceWith/FromReference
TORC2 complex7
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
normal growth on trichostatin Abit61ΔNull113
sensitive to sodium butyratebit61ΔNull93
sensitive to valproic acidbit61ΔNull155
viable vegetative cell populationbit61ΔNull3783

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologybit61ΔNull3092
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
116127791611047

UTRs

Region Coordinates Reference
five_prime_UTR1612779..1612565PMID:21511999
three_prime_UTR1611295..1611047PMID:21511999
exon1612564..1611296
mRNA1612779..1611047
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08539 Pfam IPR013745 HbrB-like 211 366 2
PTHR32428:SF2 HMMPANTHER 1 420 1
PTHR32428 HMMPANTHER 1 420 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.05 Da
Charge 6.50
Isoelectric point 8.23
Molecular weight 46.86 kDa
Number of residues 422
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
level fluctuates during mitotic cell cycleS132
present during mitotic M phaseS132
Annotation ExtensionEvidenceResidueReference
level fluctuates during mitotic cell cycle experimental evidence S132 PMID:24763107
present during mitotic M phase experimental evidence S132 PMID:21712547
phosphorylated residue 1923
Annotation ExtensionEvidenceResidueReference
NAS PMID:18076567
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2089during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1602during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1642during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2503during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1744during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.7during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.62during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bytor1phosphatidylinositol kinase Tor1 Affinity Capture-MSPMID:18076573
affinity captured bytor1phosphatidylinositol kinase Tor1 Affinity Capture-WesternPMID:22645648
affinity captured bytor1phosphatidylinositol kinase Tor1 Affinity Capture-WesternPMID:21625337
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withapl5AP-3 adaptor complex subunit Apl5 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withatd1aldehyde dehydrogenase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withlos1karyopherin exportin T Los1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withSPCC18.17cproteasome assembly chaperone (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPCC965.13pyridoxal family transmembrane transporter (predicted) Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPCC777.08c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC777.08c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC777.08c BioGRID Interaction Datasets
Expression Viewer SPCC777.08c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC777.08c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC777.08c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC777.08c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC777.08c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC777.08c Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC777.08c Cell Cycle Data
GEO SPCC777.08c GEO profiles
PInt SPCC777.08c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC777.08c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC777.08c Fission yeast phenotypic data & analysis
SPD / RIKEN20/20C11Orfeome Localization Data
UniProtKB/SwissProtO74547Target of rapamycin complex 2 subunit bit61
ModBaseO74547Database of comparative protein structure models
STRINGO74547Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588254TORC2 subunit Bit61
RefSeq mRNANM_001023244972h- TORC2 subunit Bit61 (bit61), mRNA
European Nucleotide ArchiveCAA20712.1ENA Protein Mapping
UniParcUPI000006A0B3UniProt Archive

Literature for bit61

Search: Europe PMC or PubMed

Release Version: PomBase:25_49 - 02 Feb 2015