bit61 (SPCC777.08c)


Gene Standard Namebit61 Characterisation Statuspublished
Systematic IDSPCC777.08c Feature Typeprotein coding
SynonymsSPCC777.08 Name Description
ProductTORC2 subunit Bit61 Product Size422aa, 46.86 kDa
Genomic Location Chromosome III, 1612779-1611047 (1733nt); CDS:1612564-1611296 (1269nt)

Ensembl Gene Location
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0031929TOR signalingTASPMID:1807657318
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0031932TORC2 complexIDAPMID:180765737
GO:0005829cytosolIDAPMID:168233722316
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002620normal growth on trichostatin ACell growth assaybit61ΔNullPECO:0000005, PECO:0000201, PECO:0000142PMID:23861937110
FYPO:0002617sensitive to sodium butyrate93
expressivity FYPO_EXT:0000003Cell growth assaybit61ΔNullPECO:0000201, PECO:0000005, PECO:0000142PMID:23861937
FYPO:0000115sensitive to valproic acid155
expressivity FYPO_EXT:0000002Cell growth assaybit61ΔNullPECO:0000201, PECO:0000005, PECO:0000142PMID:23861937
FYPO:0002060viable vegetative cell populationMicroscopybit61ΔNullPMID:204732893730
Microscopybit61ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopybit61ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
116127791611047

UTRs

Region Coordinates Reference
five_prime_UTR1612779..1612565PMID:21511999
three_prime_UTR1611295..1611047PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08539 Pfam IPR013745 HbrB-like 211 366 2
PTHR32428 HMMPANTHER 1 420 1
PTHR32428:SF2 HMMPANTHER 1 420 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.05 Da
Charge 6.50
Isoelectric point 8.23
Molecular weight 46.86 kDa
Number of residues 422
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS132PMID:217125471669
present during mitotic M phase
level fluctuates during mitotic cell cycleS132PMID:24763107
MOD:00696phosphorylated residueNASPMID:180765671921
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2089during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
1602during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
1642during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
2503during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
NDduring GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
1744during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2.7during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
0.62during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
apl5AP-3 adaptor complex subunit Apl5 (predicted) Positive GeneticPMID:22681890
SPCC965.13pyridoxal family membrane transporter (predicted) Negative GeneticPMID:22681890
SPCC18.17c26S proteasome non-ATPase regulatory subunit (predicted) Negative GeneticPMID:22681890
los1karyopherin exportin T Los1 (predicted) Positive GeneticPMID:22681890
atd1aldehyde dehydrogenase (predicted) Positive GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
tor1phosphatidylinositol kinase Tor1 Affinity Capture-MSPMID:18076573
Affinity Capture-WesternPMID:22645648
Affinity Capture-WesternPMID:21625337
External References
Database Identifier Description
NBRP SPCC777.08c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC777.08c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC777.08c BioGRID Interaction Datasets
Expression Viewer SPCC777.08c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC777.08c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC777.08c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC777.08c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC777.08c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC777.08c Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC777.08c Cell Cycle Data
GEO SPCC777.08c GEO profiles
PInt SPCC777.08c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC777.08c Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN20/20C11Orfeome Localization Data
EntrezGene2538963TORC2 subunit Bit61
WikiGene2538963TORC2 subunit Bit61
UniProtKB/SwissProtO74547Target of rapamycin complex 2 subunit bit61
ModBaseO74547Database of comparative protein structure models
STRINGO74547Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588254TORC2 subunit Bit61
RefSeq mRNANM_001023244972h- TORC2 subunit Bit61 (bit61), mRNA
European Nucleotide ArchiveCAA20712ENA Protein Mapping
European Nucleotide ArchiveCAA20712.1ENA Protein Mapping
UniParcUPI000006A0B3UniProt Archive

Literature for bit61

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014