bit61 (SPCC777.08c)


Gene Standard Namebit61 Characterisation Statuspublished
Systematic IDSPCC777.08c Feature Typeprotein coding
SynonymsSPCC777.08 Name Description
ProductTORC2 subunit Bit61 Product Size422aa, 46.86 kDa
Genomic Location Chromosome III, 1612779-1611047 (1733nt); CDS:1612564-1611296 (1269nt)

Ensembl Gene Location
GO Cellular Component
Term NameCount
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
TORC2 complex8
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
normal growth on trichostatin Abit61Δ114
sensitive to sodium butyratebit61Δ93
sensitive to valproic acidbit61Δ155
viable vegetative cell populationbit61Δ3850

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologybit61Δ3102
Target Of
OntologyRelationshipGeneProduct
FYPO localization affected by mutation in tor1 serine/threonine protein kinase Tor1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1612779..1611047
mRNA1612779..1611047
5' UTR1612779..1612565PMID:21511999
CDS1612564..1611296
3' UTR1611295..1611047PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08539 Pfam IPR013745 TORC2 component Bit61/PRR5 213 364 2
PTHR32428 HMMPANTHER 1 418 2
PTHR32428:SF2 HMMPANTHER 1 418 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.05 Da
Charge 6.50
Codon Adaptation Index 0.38
Isoelectric point 8.23
Molecular weight 46.86 kDa
Number of residues 422
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during mitotic M phaseS132
S58, S132, S281
level fluctuates during mitotic cell cycleS132
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S132 PMID:21712547
IDA S58 PMID:25720772
level fluctuates during mitotic cell cycle experimental evidence S132 PMID:24763107
IDA S132 PMID:25720772
IDA S281 PMID:25720772
O-phospho-L-threonineT60 1085
Annotation ExtensionEvidenceResidueReference
IDA T60 PMID:25720772
phosphorylated residue 2511
Annotation ExtensionEvidenceResidueReference
NAS PMID:18076567
O-phosphorylated residueS26,S29, S26,S31, T25,S26, T25,S29 2457
Annotation ExtensionEvidenceResidueReference
IDA T25,S26 PMID:25720772
IDA S26,S29 PMID:25720772
IDA S26,S31 PMID:25720772
IDA T25,S29 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for bit61 (SPCC777.08c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2089during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1602during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1642during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2503during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1744during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.7during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.62during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPCC777.08c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withatg11autophagy protein Atg11 Two-hybridPMID:26771498
binds DNA-binding domain construct withsec6exocyst complex subunit Sec6 Two-hybridPMID:26771498
binds DNA-binding domain construct withcaf4CCR4-Not complex subunit Caf4/Mdv1 (predicted) Two-hybridPMID:26771498
binds DNA-binding domain construct withmeu23mug2/mug135/meu2 family Two-hybridPMID:26771498
affinity captured bytor1serine/threonine protein kinase Tor1 Affinity Capture-WesternPMID:22645648
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPCC777.08c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withlos1karyopherin exportin T Los1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withapl5AP-3 adaptor complex subunit Apl5 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withatd1aldehyde dehydrogenase (predicted) Positive GeneticPMID:22681890
negative genetic interaction withSPCC18.17cproteasome assembly chaperone (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPCC965.13pyridoxal family transmembrane transporter (predicted) Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPCC777.08c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC777.08c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC777.08c BioGRID Interaction Datasets
Expression Viewer SPCC777.08c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC777.08c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC777.08c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC777.08c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC777.08c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC777.08c Transcriptome Viewer (Bähler Lab)
GEO SPCC777.08c GEO profiles
PInt SPCC777.08c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC777.08c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC777.08c Fission yeast phenotypic data & analysis
Cyclebase SPCC777.08c.1 Cell Cycle Data
SPD / RIKEN20/20C11Orfeome Localization Data
UniProtKB/SwissProtO74547Target of rapamycin complex 2 subunit bit61
ModBaseO74547Database of comparative protein structure models
STRINGO74547Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588254TORC2 subunit Bit61
RefSeq mRNANM_001023244972h- TORC2 subunit Bit61 (bit61), mRNA
European Nucleotide ArchiveCAA20712.1ENA Protein Mapping
UniParcUPI000006A0B3UniProt Archive

Literature for bit61

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016