ast1 (SPCC962.05)

Report an error | Help

Gene Standard Nameast1 Characterisation Statusconserved unknown
Systematic IDSPCC962.05 Feature Typeprotein coding
Synonyms Name Description
Productasteroid homolog, XP-G family protein Product Size519aa, 59.95 kDa
Genomic Location Chromosome III, 553995-556291 (2297nt); CDS:554521-556200 (1680nt)

Ensembl Gene Location
  1. bir1
  2. cut15
  3. rps1201
  4. ast1
  5. bpb1
  6. SPCC1672.01
  7. sap1
GO Molecular Function
Report an error | Help
Term NameCount
nuclease activity103
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Report an error | Help
Term NameCount
cytosol2298
Annotation ExtensionEvidenceWith/FromReference
mitochondrion749
Annotation ExtensionEvidenceWith/FromReference
site of double-strand break54
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Report an error | Help
Term NameGenotypesCount
normal growth during cellular response to UVast1Δ81
normal growth on methyl methanesulfonateast1Δ60
viable vegetative cell populationast1Δ3862

Cell Phenotype

Report an error | Help
Term NameGenotypesCount
viable vegetative cell with normal cell morphologyast1Δ3103

FYPO Multi-allele Phenotypes

Population Phenotype

Report an error | Help
Term NameGenotypes
inviable vegetative cell populationast1Δ, rad2Δ, exo1+
normal growth on methyl methanesulfonatechk1-E472D (E472D), ast1+
mre11Δ, ast1Δ, pku70Δ
sensitive to methyl methanesulfonateast1Δ, mre11Δ
chk1Δ, ast1Δ
chk1-E472D (E472D), ast1ND (D158A, D160A, D179A, D181A)
chk1-D155A (D155A), ast1+
ast1Δ, exo1Δ
ast1Δ, rad2Δ
sensitive to UV during vegetative growthchk1Δ, ast1Δ

Cell Phenotype

Report an error | Help
Term NameGenotypes
abnormal double-strand break processingast1Δ, rad2Δ, exo1+
abnormal mitotic sister chromatid segregationrad2Δ, ast1Δ, exo1+
cutast1Δ, exo1Δ, rad2Δ
decreased protein localization to double-strand break site
affects localization of rad51rad2Δ, ast1Δ, exo1+
affects localization of ssb1rad2Δ, ast1Δ, exo1+
decreased protein phosphorylation during cellular response to DNA damage
affecting chk1rad2Δ, ast1Δ, exo1+
increased protein phosphorylation during vegetative growth
affecting chk1ast1Δ, rad2Δ
affecting chk1ast1Δ, exo1Δ
inviable elongated vegetative cellast1Δ, exo1Δ, rad2Δ
normal mitotic DNA damage checkpoint during cellular response to UVast1Δ, rad2Δ, exo1+
normal protein phosphorylation during cellular response to DNA damage
affecting chk1chk1-E472D (E472D), ast1+
Transcript
Report an error | Help
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons553995..554744, 554865..556291
Intron554745..554864
mRNA553995..556291
5' UTR553995..554520PMID:21511999
CDS554521..554744, 554865..556200
3' UTR556201..556291PMID:21511999
Protein Features
Report an error | Help

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF12813 Pfam 107 343 1
PTHR15665 HMMPANTHER IPR026832 Asteroid 2 201 1
SSF88723 SuperFamily IPR029060 PIN domain-like 2 167 10

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000281XP-G familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000281

Protein Properties

Ave. residue weight 115.51 Da
Charge 5.50
Codon Adaptation Index 0.38
Isoelectric point 7.20
Molecular weight 59.95 kDa
Number of residues 519
Sequence
Report an error | Help
Gene Expression
Report an error | Help

Quantitative Gene Expression

View graphical display of gene expression data for ast1 (SPCC962.05)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.44during GO:0072690PECO:0000126,
PECO:0000005		population wideexperimental evidencePMID:23101633
0.15during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005		population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog600
predominantly single copy (one to one)3087
conserved in fungi4608
conserved in eukaryotes4516
conserved in metazoa3498
conserved in vertebrates3473
conserved in eukaryotes only2525
Orthologs
Report an error | Help

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPCC962.05 into the Query Builder
View these interactions in esyN

Report an error | Help
Gene Product Evidence Reference
enhances phenotype ofexo1exonuclease I Exo1 Phenotypic EnhancementPMID:23211746
enhances phenotype ofrad2FEN-1 endonuclease Rad2 Phenotypic EnhancementPMID:23211746
rescuesrad51RecA family recombinase Rad51/Rhp51 Phenotypic SuppressionPMID:23211746
rescuesrad2FEN-1 endonuclease Rad2 Phenotypic SuppressionPMID:23211746
rescuesexo1exonuclease I Exo1 Phenotypic SuppressionPMID:23211746
overexpression rescueschk1Chk1 protein kinase Dosage RescuePMID:23211746
overexpression rescuescdc27DNA polymerase delta subunit Cdc27 Dosage RescuePMID:23211746
synthetic growth defect withmre11Mre11 nuclease Synthetic Growth DefectPMID:23211746
External References
Report an error | Help
Database Identifier Description
NBRP SPCC962.05 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC962.05 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC962.05 BioGRID Interaction Datasets
Expression Viewer SPCC962.05 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC962.05 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC962.05 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC962.05 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC962.05 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC962.05 Transcriptome Viewer (Bähler Lab)
GEO SPCC962.05 GEO profiles
PInt SPCC962.05 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC962.05 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC962.05 Fission yeast phenotypic data & analysis
Cyclebase SPCC962.05.1 Cell Cycle Data
SPD / RIKEN35/35B08Orfeome Localization Data
UniProtKB/SwissProtO14061Asteroid homolog 1
ModBaseO14061Database of comparative protein structure models
STRINGO14061Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587870hypothetical protein SPCC962.05
RefSeq mRNANM_001022862972h- hypothetical protein (ast1), mRNA
European Nucleotide ArchiveCAA20437.2ENA Protein Mapping
UniParcUPI0001B06259UniProt Archive

Literature for ast1
Report an error | Help

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017