vma8 (SPCC965.03)


Gene Standard Namevma8 Characterisation Statusbiological_role_inferred
Systematic IDSPCC965.03 Feature Typeprotein coding
Synonyms Name Description
ProductV-type ATPase V1 subunit D (predicted) Product Size285aa, 32.22 kDa
Genomic Location Chromosome III, 2285010-2286855 (1846nt); CDS:2285073-2286229 (1157nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingICGO:0046933GO_REF:0000001559
GO:0046961proton-transporting ATPase activity, rotational mechanism29
contributes_toISMPFAM:PF01813GO_REF:0000001
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0015991ATP hydrolysis coupled proton transportICGO:0016471GO_REF:000000117
GO:0007035vacuolar acidificationISOSGD:S000000777PMID:1629172523
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032153cell division siteIDAPMID:16823372295
GO:0005829cytosolIDAPMID:168233722316
GO:0000329fungal-type vacuole membraneIDAPMID:1682337291
GO:0000221vacuolar proton-transporting V-type ATPase, V1 domainISOSGD:S000000777GO_REF:00000248
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopyvma8ΔNullPMID:204732891315
Microscopyvma8ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002151inviable spore457
penetrance FYPO_EXT:0000001Microscopyvma8ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
122850102285113
222851542285271
322855312286855

UTRs

Region Coordinates Reference
five_prime_UTR2285010..2285072PMID:21511999
three_prime_UTR2286230..2286855PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01813 Pfam IPR002699 ATPase, V1 complex, subunit D 14 209 1
PTHR11671 HMMPANTHER IPR002699 ATPase, V1 complex, subunit D 2 224 1
Coil ncoils Rabaptin coiled-coil domain 179 200 968
Coil ncoils Rabaptin coiled-coil domain 37 58 968
TIGR00309 tigrfam IPR002699 ATPase, V1 complex, subunit D 7 213 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.05 Da
Charge -3.50
Isoelectric point 5.34
Molecular weight 32.22 kDa
Number of residues 285
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS219PMID:217125471663
present during mitotic M phase
present during mitotic M phaseS259PMID:21712547
MOD:00696phosphorylated residueNASPMID:195477441915
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
42543during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
45754during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
43651during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
11352.81during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
41928during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
47668during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
8058.4during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
4.4during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPCC965.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC965.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC965.03 BioGRID Interaction Datasets
Expression Viewer SPCC965.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC965.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC965.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC965.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC965.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC965.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC965.03 Cell Cycle Data
GEO SPCC965.03 GEO profiles
PInt SPCC965.03 Protein-Protein Interaction Predictor (Bähler Lab)
IntEnz3.6.3.14Integrated relational Enzyme database
Rhea3.6.3.14Annotated reactions database
WikiGene2539581V-type ATPase subunit D (predicted)
EntrezGene2539581V-type ATPase subunit D (predicted)
SPD / RIKEN18/18C08Orfeome Localization Data
UniProtKB/SwissProtO59823V-type proton ATPase subunit D
ModBaseO59823Database of comparative protein structure models
STRINGO59823Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588513V-type ATPase subunit D (predicted)
RefSeq mRNANM_001023502972h- V-type ATPase subunit D (predicted) (vma8), mRNA
European Nucleotide ArchiveCAA19063ENA Protein Mapping
European Nucleotide ArchiveCAA19063.1ENA Protein Mapping
UniParcUPI0000138199UniProt Archive

Literature for vma8

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014