vma8 (SPCC965.03)


Gene Standard Namevma8 Characterisation Statusbiological role inferred
Systematic IDSPCC965.03 Feature Typeprotein coding
Synonyms Name Description
ProductV-type ATPase V1 subunit D (predicted) Product Size285aa, 32.22 kDa
Genomic Location Chromosome III, 2285010-2286855 (1846nt); CDS:2285073-2286229 (1157nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding559
Annotation ExtensionEvidenceWith/FromReference
proton-transporting ATPase activity, rotational mechanism29
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
ATP hydrolysis coupled proton transport17
Annotation ExtensionEvidenceWith/FromReference
vacuolar acidification17
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell division site309
Annotation ExtensionEvidenceWith/FromReference
cytosol2307
Annotation ExtensionEvidenceWith/FromReference
fungal-type vacuole membrane89
Annotation ExtensionEvidenceWith/FromReference
vacuolar proton-transporting V-type ATPase, V1 domain8
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Single-allele Phenotypes

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationvma8Δ1444

Cell Phenotype

Term NameGenotypesCount
inviable sporevma8Δ474
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2285010..2285113, 2285154..2285271, 2285531..2286855
Intron2285114..2285153, 2285272..2285530
mRNA2285010..2286855
5' UTR2285010..2285072PMID:21511999
CDS2285073..2285113, 2285154..2285271, 2285531..2286229
3' UTR2286230..2286855PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01813 Pfam IPR002699 ATPase, V1 complex, subunit D 16 209 1
PTHR11671:SF1 HMMPANTHER 2 265 1
PTHR11671 HMMPANTHER IPR002699 ATPase, V1 complex, subunit D 2 265 1
Coil ncoils Predicted coiled-coil protein (DUF2205) 179 199 1048
Coil ncoils Predicted coiled-coil protein (DUF2205) 37 57 1048
MF_00271 hamap IPR002699 ATPase, V1 complex, subunit D 6 214 1
TIGR00309 tigrfam IPR002699 ATPase, V1 complex, subunit D 7 213 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.05 Da
Charge -3.50
Isoelectric point 5.34
Molecular weight 32.22 kDa
Number of residues 285
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1674
present during mitotic M phaseS219
present during mitotic M phaseS259
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S219 PMID:21712547
present during mitotic M phase experimental evidence S259 PMID:21712547
phosphorylated residue 1930
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
45754during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
47668during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
43651during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
42543during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
41928during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
8058.4during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
11352.81during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.4during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3090
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3421
conserved in vertebrates3396
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


External References
Database Identifier Description
NBRP SPCC965.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC965.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC965.03 BioGRID Interaction Datasets
Expression Viewer SPCC965.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC965.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC965.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC965.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC965.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC965.03 Transcriptome Viewer (Bähler Lab)
GEO SPCC965.03 GEO profiles
PInt SPCC965.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCC965.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCC965.03 Fission yeast phenotypic data & analysis
Cyclebase SPCC965.03.1 Cell Cycle Data
IntEnz3.6.3.14Integrated relational Enzyme database
Rhea3.6.3.14Annotated reactions database
SPD / RIKEN18/18C08Orfeome Localization Data
UniProtKB/SwissProtO59823V-type proton ATPase subunit D
ModBaseO59823Database of comparative protein structure models
STRINGO59823Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588513V-type ATPase V1 subunit D (predicted)
RefSeq mRNANM_001023502972h- V-type ATPase V1 subunit D (predicted) (vma8), mRNA
European Nucleotide ArchiveCU329672ENA EMBL mapping
European Nucleotide ArchiveCAA19063ENA Protein Mapping
European Nucleotide ArchiveCAA19063.1ENA Protein Mapping
UniParcUPI0000138199UniProt Archive

Literature for vma8

Search: Europe PMC or PubMed

Release Version: PomBase:29_56 - 12 Nov 2015