vma8 (SPCC965.03)


Gene Standard Namevma8 Characterisation Statusbiological_role_inferred
Systematic IDSPCC965.03 Feature Typeprotein coding
Synonyms Name Description
ProductV-type ATPase V1 subunit D (predicted) Product Size285aa, 32.22 kDa
Genomic Location Chromosome III, 2285010-2286855 (1846nt); CDS:2285073-2286229 (1157nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingICGO:0046933GO_REF:0000001559
GO:0046961proton-transporting ATPase activity, rotational mechanism29
contributes_toISMPFAM:PF01813GO_REF:0000001
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0015991ATP hydrolysis coupled proton transportICGO:0016471GO_REF:000000117
GO:0007035vacuolar acidificationISOSGD:S000000777PMID:1629172523
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032153cell division siteIDAPMID:16823372292
GO:0005829cytosolIDAPMID:168233722317
GO:0000329fungal-type vacuole membraneIDAPMID:1682337291
GO:0000221vacuolar proton-transporting V-type ATPase, V1 domainISOSGD:S000000777GO_REF:00000248
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopyvma8ΔNullPMID:204732891309
Microscopyvma8ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002151inviable sporeMicroscopyvma8ΔNullPECO:0000005, PECO:0000137PMID:23697806447
penetrance FYPO_EXT:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
122850102285113
222851542285271
322855312286855

UTRs

Region Start End Reference
three_prime_UTR22862302286855PMID:21511999
five_prime_UTR22850102285072PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01813 Pfam IPR002699 ATPase, V1 complex, subunit D 14 209 1
PTHR11671 HMMPANTHER IPR002699 ATPase, V1 complex, subunit D 1 285 1
Coil ncoils Rabaptin coiled-coil domain 37 58 975
Coil ncoils Rabaptin coiled-coil domain 179 200 975
Low complexity (SEG) seg 190 199
Low complexity (SEG) seg 268 282
Low complexity (SEG) seg 12 24
TIGR00309 tigrfam IPR002699 ATPase, V1 complex, subunit D 7 213 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.05 Da
Charge -3.50
Isoelectric point 5.34
Molecular weight 32.22 kDa
Number of residues 285
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residuePMID:195477441192
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
11352.81during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
8058.4during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
4.4during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPCC965.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCC965.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCC965.03 BioGRID Interaction Datasets
Expression Viewer SPCC965.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCC965.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCC965.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCC965.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCC965.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCC965.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCC965.03 Cell Cycle Data
GEO SPCC965.03 GEO profiles
PInt SPCC965.03 Protein-Protein Interaction Predictor (Bähler Lab)
IntEnz3.6.3.14Integrated relational Enzyme database
Rhea3.6.3.14Annotated reactions database
WikiGene2539581V-type ATPase subunit D (predicted)
EntrezGene2539581V-type ATPase subunit D (predicted)
SPD / RIKEN18/18C08Orfeome Localization Data
UniProtKB/SwissProtO59823V-type proton ATPase subunit D
ModBaseO59823Database of comparative protein structure models
StringO59823Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588513V-type ATPase subunit D (predicted)
RefSeq mRNANM_001023502972h- V-type ATPase subunit D (predicted) (vma8), mRNA
European Nucleotide ArchiveCAA19063ENA Protein Mapping
UniParcUPI0000138199UniProt Archive

Literature for vma8

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014