tho1 (SPCP25A2.03)


Gene Standard Nametho1 Characterisation Statusbiological role inferred
Systematic IDSPCP25A2.03 Feature Typeprotein coding
Synonyms Name Description
ProductTHO complex subunit Tho1 (predicted) Product Size752aa, 85.24 kDa
Genomic Location Chromosome III, 1182719-1185336 (2618nt); CDS:1182796-1185168 (2373nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
mRNA export from nucleus39
Annotation ExtensionEvidenceWith/FromReference
transcription elongation from RNA polymerase II promoter32
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
THO complex5
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationtho1Δ1455

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic cell cycletho1Δ894
inviable elongated tapered vegetative celltho1Δ3
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1182719..1182990, 1183050..1183940, 1183996..1185336
Intron1182991..1183049, 1183941..1183995
mRNA1182719..1185336
5' UTR1182719..1182795PMID:21511999
CDS1182796..1182990, 1183050..1183940, 1183996..1185168
3' UTR1185169..1185336PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF11957 Pfam IPR021861 THO complex, subunit THOC1 80 546 1
PTHR13265:SF0 HMMPANTHER 35 647 1
PTHR13265 HMMPANTHER 35 647 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.35 Da
Charge -27.50
Codon Adaptation Index 0.39
Isoelectric point 4.80
Molecular weight 85.24 kDa
Number of residues 752
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during mitotic M phaseS706
present during cellular response to thiabendazole, mitotic M phaseS707
present during mitotic M phaseS661
present during mitotic M phaseS737
present during mitotic M phaseS205
present during mitotic M phaseS643
present during cellular response to thiabendazoleS471
present during mitotic M phaseS582
present during cellular response to thiabendazoleS202
present during mitotic M phaseS733
present during mitotic M phaseS738
S202, S471, S477, S643, S649, S656, S661, S706, S707, S711, S737, S738
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S706 PMID:21712547
present during cellular response to thiabendazole IDA S707 PMID:18257517
present during mitotic M phase experimental evidence S661 PMID:21712547
present during mitotic M phase experimental evidence S737 PMID:21712547
present during mitotic M phase experimental evidence S205 PMID:21712547
present during mitotic M phase experimental evidence S643 PMID:21712547
present during cellular response to thiabendazole IDA S471 PMID:18257517
present during mitotic M phase experimental evidence S707 PMID:21712547
present during mitotic M phase experimental evidence S582 PMID:21712547
present during cellular response to thiabendazole IDA S202 PMID:18257517
present during mitotic M phase experimental evidence S733 PMID:21712547
present during mitotic M phase experimental evidence S738 PMID:21712547
IDA S737 PMID:25720772
IDA S706 PMID:25720772
IDA S477 PMID:25720772
IDA S656 PMID:25720772
experimental evidence S202 PMID:24763107
experimental evidence S706 PMID:24763107
experimental evidence S643 PMID:24763107
IDA S471 PMID:25720772
experimental evidence S707 PMID:24763107
IDA S707 PMID:25720772
IDA S661 PMID:25720772
IDA S738 PMID:25720772
IDA S711 PMID:25720772
IDA S643 PMID:25720772
IDA S649 PMID:25720772
experimental evidence S661 PMID:24763107
O-phospho-L-threonine 1085
present during mitotic M phaseT586
T586
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T586 PMID:21712547
experimental evidence T586 PMID:24763107
phosphorylated residue 2511
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
O-phosphorylated residueS707,S710, S737,S738 2457
Annotation ExtensionEvidenceResidueReference
IDA S707,S710 PMID:25720772
IDA S737,S738 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for tho1 (SPCP25A2.03)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2152during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1629during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1976during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2307during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1885during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1742.34during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1752.13during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.64during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancer148
Taxonomic Conservation
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog600
predominantly single copy (one to one)3093
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPCP25A2.03 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byepe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
affinity captured byimp1importin alpha Affinity Capture-MSPMID:25963819
External References
Database Identifier Description
NBRP SPCP25A2.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCP25A2.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCP25A2.03 BioGRID Interaction Datasets
Expression Viewer SPCP25A2.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCP25A2.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCP25A2.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCP25A2.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCP25A2.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCP25A2.03 Transcriptome Viewer (Bähler Lab)
GEO SPCP25A2.03 GEO profiles
PInt SPCP25A2.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCP25A2.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCP25A2.03 Fission yeast phenotypic data & analysis
Cyclebase SPCP25A2.03.1 Cell Cycle Data
SPD / RIKEN28/28E03Orfeome Localization Data
UniProtKB/SwissProtQ9URT2Uncharacterized protein P25A2.03
ModBaseQ9URT2Database of comparative protein structure models
STRINGQ9URT2Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588092THO complex subunit (predicted)
RefSeq mRNANM_001023083972h- THO complex subunit (predicted) (SPCP25A2.03), mRNA
European Nucleotide ArchiveCAB62828.1ENA Protein Mapping
UniParcUPI00000696C5UniProt Archive

Literature for tho1

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016