SPCP25A2.03


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPCP25A2.03 Feature Typeprotein coding
Synonyms Name Description
ProductTHO complex subunit (predicted) Product Size752aa, 85.24 kDa
Genomic Location Chromosome III, 1182719-1185336 (2618nt); CDS:1182796-1185168 (2373nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
mRNA export from nucleus47
Annotation ExtensionEvidenceWith/FromReference
transcription elongation from RNA polymerase II promoter34
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
THO complex5
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationSPCP25A2.03ΔNull1427

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic cell cycleSPCP25A2.03ΔNull794
inviable elongated tapered vegetative cell2
penetrance FYPO_EXT:0000001SPCP25A2.03ΔNull
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
111827191182990
211830501183940
311839961185336

UTRs

Region Coordinates Reference
five_prime_UTR1182719..1182795PMID:21511999
three_prime_UTR1185169..1185336PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF11957 Pfam IPR021861 THO complex, subunit THOC1 78 562 1
PTHR13265 HMMPANTHER 17 637 1
PTHR13265:SF0 HMMPANTHER 17 637 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.35 Da
Charge -27.50
Isoelectric point 4.80
Molecular weight 85.24 kDa
Number of residues 752
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS643
present during mitotic M phaseS706
present during cellular response to thiabendazole, mitotic M phaseS707
present during mitotic M phaseS737
present during cellular response to thiabendazoleS202
S202, S643, S661, S706, S707
present during mitotic M phaseS738
present during mitotic M phaseS582
present during mitotic M phaseS205
present during mitotic M phaseS733
present during mitotic M phaseS661
present during cellular response to thiabendazoleS471
Annotation ExtensionEvidenceResidueReference
experimental evidence S202 PMID:24763107
present during cellular response to thiabendazole IDA S202 PMID:18257517
present during mitotic M phase experimental evidence S205 PMID:21712547
present during cellular response to thiabendazole IDA S471 PMID:18257517
present during mitotic M phase experimental evidence S582 PMID:21712547
experimental evidence S643 PMID:24763107
present during mitotic M phase experimental evidence S643 PMID:21712547
experimental evidence S661 PMID:24763107
present during mitotic M phase experimental evidence S661 PMID:21712547
experimental evidence S706 PMID:24763107
present during mitotic M phase experimental evidence S706 PMID:21712547
experimental evidence S707 PMID:24763107
present during cellular response to thiabendazole IDA S707 PMID:18257517
present during mitotic M phase experimental evidence S707 PMID:21712547
present during mitotic M phase experimental evidence S733 PMID:21712547
present during mitotic M phase experimental evidence S737 PMID:21712547
present during mitotic M phase experimental evidence S738 PMID:21712547
O-phospho-L-threonineT586 693
present during mitotic M phaseT586
Annotation ExtensionEvidenceResidueReference
experimental evidence T586 PMID:24763107
present during mitotic M phase experimental evidence T586 PMID:21712547
phosphorylated residue 1922
Annotation ExtensionEvidenceResidueReference
NAS PMID:18257517
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2152during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1629during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1976during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2307during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1742.34during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1885during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1752.13during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.64during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancer142
Species Distribution
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog597
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
External References
Database Identifier Description
NBRP SPCP25A2.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCP25A2.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCP25A2.03 BioGRID Interaction Datasets
Expression Viewer SPCP25A2.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCP25A2.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCP25A2.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCP25A2.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCP25A2.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCP25A2.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCP25A2.03 Cell Cycle Data
GEO SPCP25A2.03 GEO profiles
PInt SPCP25A2.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCP25A2.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCP25A2.03 Fission yeast phenotypic data & analysis
SPD / RIKEN28/28E03Orfeome Localization Data
UniProtKB/SwissProtQ9URT2Uncharacterized protein P25A2.03
ModBaseQ9URT2Database of comparative protein structure models
STRINGQ9URT2Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588092THO complex subunit (predicted)
RefSeq mRNANM_001023083972h- THO complex subunit (predicted) (SPCP25A2.03), mRNA
European Nucleotide ArchiveCAB62828.1ENA Protein Mapping
UniParcUPI00000696C5UniProt Archive

Literature for SPCP25A2.03

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015