SPCP25A2.03


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPCP25A2.03 Feature Typeprotein coding
Synonyms Name Description
ProductTHO complex subunit (predicted) Product Size752aa, 85.24 kDa
Genomic Location Chromosome III, 1182719-1185336 (2618nt); CDS:1182796-1185168 (2373nt)

Ensembl Gene Location
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006406mRNA export from nucleusICGO:0000347GO_REF:000000151
GO:0006368transcription elongation from RNA polymerase II promoterISSUniProtKB:Q8R3N6GO_REF:000000125
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000347THO complexISSUniProtKB:Q8R3N6GO_REF:00000015
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopySPCP25A2.03ΔNullPECO:0000005, PECO:0000137PMID:236978061331
MicroscopySPCP25A2.03ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopySPCP25A2.03ΔNullPECO:0000005, PECO:0000137PMID:23697806638
FYPO:0002242inviable elongated tapered vegetative cell2
penetrance FYPO_EXT:0000001MicroscopySPCP25A2.03ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
111827191182990
211830501183940
311839961185336

UTRs

Region Coordinates Reference
five_prime_UTR1182719..1182795PMID:21511999
three_prime_UTR1185169..1185336PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF11957 Pfam IPR021861 THO complex, subunit THOC1 78 562 1
PTHR13265 HMMPANTHER 17 637 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.35 Da
Charge -27.50
Isoelectric point 4.80
Molecular weight 85.24 kDa
Number of residues 752
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS643PMID:247631071669
S202PMID:24763107
present during cellular response to thiabendazoleIDAS471PMID:18257517
present during mitotic M phaseS737PMID:21712547
present during mitotic M phaseS707PMID:21712547
present during mitotic M phaseS582PMID:21712547
present during mitotic M phaseS661PMID:21712547
present during mitotic M phaseS738PMID:21712547
present during cellular response to thiabendazoleIDAS707PMID:18257517
present during mitotic M phaseS205PMID:21712547
S661PMID:24763107
present during mitotic M phaseS643PMID:21712547
present during cellular response to thiabendazoleIDAS202PMID:18257517
present during mitotic M phaseS733PMID:21712547
present during mitotic M phaseS706PMID:21712547
S706PMID:24763107
S707PMID:24763107
MOD:00047O-phospho-L-threonineT586PMID:24763107689
present during mitotic M phaseT586PMID:21712547
MOD:00696phosphorylated residueNASPMID:182575171921
NASPMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2152during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
1629during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
1976during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
2307during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
1742.34during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
1885during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
1752.13during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
0.64during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancer142
Species Distribution
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog579
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
External References
Database Identifier Description
NBRP SPCP25A2.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCP25A2.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCP25A2.03 BioGRID Interaction Datasets
Expression Viewer SPCP25A2.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCP25A2.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCP25A2.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCP25A2.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCP25A2.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCP25A2.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPCP25A2.03 Cell Cycle Data
GEO SPCP25A2.03 GEO profiles
PInt SPCP25A2.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCP25A2.03 Peptides identified in tandem mass spectrometry proteomics experiments
WikiGene2539396THO complex subunit (predicted)
EntrezGene2539396THO complex subunit (predicted)
SPD / RIKEN28/28E03Orfeome Localization Data
UniProtKB/SwissProtQ9URT2Uncharacterized protein P25A2.03
ModBaseQ9URT2Database of comparative protein structure models
STRINGQ9URT2Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_588092THO complex subunit (predicted)
RefSeq mRNANM_001023083972h- THO complex subunit (predicted) (SPCP25A2.03), mRNA
European Nucleotide ArchiveCAB62828ENA Protein Mapping
European Nucleotide ArchiveCAB62828.1ENA Protein Mapping
UniParcUPI00000696C5UniProt Archive

Literature for SPCP25A2.03

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014