med31 (SPCP31B10.03c)


Gene Standard Namemed31 Characterisation Statuspublished
Systematic IDSPCP31B10.03c Feature Typeprotein coding
Synonymssep10, soh1 Name Description
Productmediator complex subunit Med31 Product Size139aa, 16.90 kDa
Genomic Location Chromosome III, 526837-525859 (979nt); CDS:526690-526271 (420nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0001104RNA polymerase II transcription cofactor activityIEAIPR008831GO_REF:000000218
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006357regulation of transcription from RNA polymerase II promoterICGO:0016592GO_REF:0000001261
GO:0006366transcription from RNA polymerase II promoterIPIGO:0016592PMID:15356001137
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722316
GO:0016592mediator complexIGIsep11PMID:1247145322
IDAPMID:15356001
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000155increased flocculationnot recorded (unrecorded)PMID:1831010212
FYPO:0002060viable vegetative cell populationMicroscopymed31ΔNullPMID:204732893760
Microscopymed31ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopymed31ΔNullPECO:0000005, PECO:0000137PMID:23697806624
FYPO:0000117abnormal septationnot recorded (unrecorded)PMID:1039244540
FYPO:0000280sterilemed31ΔNullPMID:1535600147
FYPO:0002457viable curved elongated vegetative cell with abnormal septum1
penetrance FYPO_EXT:0000001Microscopymed31ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1526837525859

UTRs

Region Coordinates Reference
five_prime_UTR526837..526691PMID:21511999
three_prime_UTR526270..525859PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF05669 Pfam IPR008831 Mediator complex, subunit Med31 13 108 1
PTHR13186 HMMPANTHER IPR008831 Mediator complex, subunit Med31 12 139 1
PTHR13186:SF0 HMMPANTHER 12 139 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 121.55 Da
Charge -10.00
Isoelectric point 4.34
Molecular weight 16.90 kDa
Number of residues 139
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
5440during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
5086during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
4549during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
NDduring GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
4969during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
4463during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
2.4during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
deletion mutant expression profiling PMID:15829570 PMID:1792223679
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
sep11mediator complex subunit Pmc6 Synthetic LethalityPMID:12471453
pmh1transcription factor TFIIH complex subunit Pmh1 Synthetic LethalityPMID:15829570
ste11transcription factor Ste11 Phenotypic SuppressionPMID:12471453
med8mediator complex subunit Med8 Synthetic LethalityPMID:17922236
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
med7mediator complex subunit Med7 Co-purificationPMID:18310102
Affinity Capture-WesternPMID:15356001
External References
Database Identifier Description
NBRP SPCP31B10.03c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCP31B10.03c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCP31B10.03c BioGRID Interaction Datasets
Expression Viewer SPCP31B10.03c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCP31B10.03c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCP31B10.03c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCP31B10.03c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCP31B10.03c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCP31B10.03c Transcriptome Viewer (Bähler Lab)
Cyclebase SPCP31B10.03c Cell Cycle Data
GEO SPCP31B10.03c GEO profiles
PInt SPCP31B10.03c Protein-Protein Interaction Predictor (Bähler Lab)
EntrezGene2539504mediator complex subunit Med31
WikiGene2539504mediator complex subunit Med31
SPD / RIKEN03/03D03Orfeome Localization Data
UniProtKB/SwissProtQ9USH1Mediator of RNA polymerase II transcription subunit 31
ModBaseQ9USH1Database of comparative protein structure models
STRINGQ9USH1Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587859mediator complex subunit Med31
RefSeq mRNANM_001022852972h- mediator complex subunit Med31 (med31), mRNA
European Nucleotide ArchiveAAG02413ENA Protein Mapping
European Nucleotide ArchiveAAG02413.1ENA Protein Mapping
European Nucleotide ArchiveCAB58369ENA Protein Mapping
European Nucleotide ArchiveCAB58369.1ENA Protein Mapping
UniParcUPI000006BB93UniProt Archive

Literature for med31

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014