med31 (SPCP31B10.03c)


Gene Standard Namemed31 Characterisation Statuspublished
Systematic IDSPCP31B10.03c Feature Typeprotein coding
Synonymssep10, soh1 Name Description
Productmediator complex subunit Med31 Product Size139aa, 16.90 kDa
Genomic Location Chromosome III, 526837-525859 (979nt); CDS:526690-526271 (420nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0001104RNA polymerase II transcription cofactor activityIEAIPR008831GO_REF:000000218
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006357regulation of transcription from RNA polymerase II promoterICGO:0016592GO_REF:0000001264
GO:0006366transcription from RNA polymerase II promoterIPIGO:0016592PMID:15356001130
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722317
GO:0016592mediator complexIDAPMID:1535600122
IGIsep11PMID:12471453
GO:0005634nucleusIDAPMID:168233722730
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000155flocculating cellsno_namePMID:1831010211
FYPO:0002060viable vegetative cell populationMicroscopymed31ΔNullPECO:0000005, PECO:0000137PMID:236978063755
Microscopymed31ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopymed31ΔNullPECO:0000005, PECO:0000137PMID:23697806564
FYPO:0000117abnormal septationno_namePMID:1039244536
FYPO:0000280sterilemed31ΔNullPMID:1535600139
FYPO:0002457viable curved elongated vegetative cell with abnormal septumMicroscopymed31ΔNullPECO:0000005, PECO:0000137PMID:236978061
penetrance FYPO_EXT:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1526837525859

UTRs

Region Start End Reference
three_prime_UTR526270525859PMID:21511999
five_prime_UTR526837526691PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF05669 Pfam IPR008831 Mediator complex, subunit Med31 13 108 1
PTHR13186:SF0 HMMPANTHER 12 139 1
PTHR13186 HMMPANTHER IPR008831 Mediator complex, subunit Med31 12 139 1
Low complexity (SEG) seg 126 137

View domain organization at Pfam

Protein Properties

Ave. residue weight 121.55 Da
Charge -10.00
Isoelectric point 4.34
Molecular weight 16.90 kDa
Number of residues 139
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
NDduring GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2.4during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
deletion mutant expression profiling PMID:15829570 PMID:1792223679
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
sep11mediator complex subunit Pmc6 Synthetic LethalityPMID:12471453
pmh1transcription factor TFIIH complex subunit Pmh1 Synthetic LethalityPMID:15829570
ste11transcription factor Ste11 Phenotypic SuppressionPMID:12471453
med8mediator complex subunit Med8 Synthetic LethalityPMID:17922236
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
med7mediator complex subunit Med7 Co-purificationPMID:18310102
Affinity Capture-WesternPMID:15356001
External References
Database Identifier Description
NBRP SPCP31B10.03c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCP31B10.03c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCP31B10.03c BioGRID Interaction Datasets
Expression Viewer SPCP31B10.03c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCP31B10.03c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCP31B10.03c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCP31B10.03c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCP31B10.03c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCP31B10.03c Transcriptome Viewer (Bähler Lab)
Cyclebase SPCP31B10.03c Cell Cycle Data
GEO SPCP31B10.03c GEO profiles
PInt SPCP31B10.03c Protein-Protein Interaction Predictor (Bähler Lab)
EntrezGene2539504mediator complex subunit Med31
WikiGene2539504mediator complex subunit Med31
SPD / RIKEN03/03D03Orfeome Localization Data
UniProtKB/SwissProtQ9USH1Mediator of RNA polymerase II transcription subunit 31
ModBaseQ9USH1Database of comparative protein structure models
StringQ9USH1Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587859mediator complex subunit Med31
RefSeq mRNANM_001022852972h- mediator complex subunit Med31 (med31), mRNA
European Nucleotide ArchiveAAG02413ENA Protein Mapping
European Nucleotide ArchiveCAB58369ENA Protein Mapping
UniParcUPI000006BB93UniProt Archive

Literature for med31

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014