med31 (SPCP31B10.03c)

Gene Standard Namemed31 Characterisation Statuspublished
Systematic IDSPCP31B10.03c Feature Typeprotein coding
Synonymssep10, soh1 Name Description
Productmediator complex subunit Med31 Product Size139aa, 16.90 kDa
Genomic Location Chromosome III, 526837-525859 (979nt); CDS:526690-526271 (420nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
RNA polymerase II transcription cofactor activity18
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
mediator complex22
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
increased flocculation16
viable vegetative cell populationmed31Δ3840

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic cell cyclemed31Δ877
abnormal septation292
viable curved elongated vegetative cell with abnormal septummed31Δ1

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
inviable vegetative cell populationmed31Δ, pmh1-26 (Q17R, N23K, F47S, T56S, L63P, E70G, G95V, K256R)

Cell Phenotype

Term NameGenotypes
inviable after spore germination, multiple cell divisionsmed31Δ, pmh1-26 (Q17R, N23K, F47S, T56S, L63P, E70G, G95V, K256R)
inviable branched, swollen, elongated, multiseptate vegetative cellmed31Δ, pmh1-26 (Q17R, N23K, F47S, T56S, L63P, E70G, G95V, K256R)
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR526837..526691PMID:21511999
3' UTR526270..525859PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF05669 Pfam IPR008831 Mediator complex, subunit Med31 15 105 1
PTHR13186:SF0 HMMPANTHER 13 104 1
PTHR13186 HMMPANTHER IPR008831 Mediator complex, subunit Med31 13 104 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 121.55 Da
Charge -10.00
Codon Adaptation Index 0.43
Isoelectric point 4.34
Molecular weight 16.90 kDa
Number of residues 139
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for med31 (SPCP31B10.03c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5440during GO:0000080PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
5086during GO:0000084PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
4969during GO:0000085PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
4463during GO:0000087PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
4549during GO:0072690PECO:0000005,
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.4during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
0.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
deletion mutant expression profiling PMID:15829570 PMID:1792223677
Taxonomic Conservation
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2506

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Load genes that interact physically with SPCP31B10.03c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bymed7mediator complex subunit Med7 Affinity Capture-WesternPMID:15356001
binds DNA-binding domain construct withmed7mediator complex subunit Med7 Two-hybridPMID:26771498
co-purifies withmed7mediator complex subunit Med7 Co-purificationPMID:18310102
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPCP31B10.03c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withpmh1transcription factor TFIIK complex complex ubiquitin-protein ligase E3 subunit, Pmh1 Synthetic LethalityPMID:15829570
synthetic lethal withmed18mediator complex subunit Med18 Synthetic LethalityPMID:12471453
synthetic lethal withmed8mediator complex subunit Med8 Synthetic LethalityPMID:17922236
rescued byste11transcription factor Ste11 Phenotypic SuppressionPMID:12471453
External References
Database Identifier Description
NBRP SPCP31B10.03c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCP31B10.03c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCP31B10.03c BioGRID Interaction Datasets
Expression Viewer SPCP31B10.03c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCP31B10.03c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCP31B10.03c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCP31B10.03c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCP31B10.03c Polyadenylation Viewer (Gullerova lab)
pombeTV SPCP31B10.03c Transcriptome Viewer (Bähler Lab)
GEO SPCP31B10.03c GEO profiles
PInt SPCP31B10.03c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCP31B10.03c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCP31B10.03c Fission yeast phenotypic data & analysis
Cyclebase SPCP31B10.03c.1 Cell Cycle Data
SPD / RIKEN03/03D03Orfeome Localization Data
UniProtKB/SwissProtQ9USH1Mediator of RNA polymerase II transcription subunit 31
ModBaseQ9USH1Database of comparative protein structure models
STRINGQ9USH1Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587859mediator complex subunit Med31
RefSeq mRNANM_001022852972h- mediator complex subunit Med31 (med31), mRNA

Literature for med31

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016