eft202 (SPCP31B10.07)


Gene Standard Nameeft202 Characterisation Statuspublished
Systematic IDSPCP31B10.07 Feature Typeprotein coding
Synonyms Name Description
Producttranslation elongation factor 2 (EF-2) Eft2,B Product Size842aa, 93.23 kDa
Genomic Location Chromosome III, 537298-539922 (2625nt); CDS:537326-539854 (2529nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
GTP binding113
Annotation ExtensionEvidenceWith/FromReference
GTPase activity62
Annotation ExtensionEvidenceWith/FromReference
translation elongation factor activity15
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic translational elongation194
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4209
Annotation ExtensionEvidenceWith/FromReference
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationeft202Δ3831

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologyeft202Δ3099
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons537298..539922
5' UTR537298..537325PMID:21511999
3' UTR539855..539922PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03144 Pfam IPR004161 Translation elongation factor EFTu/EF1A, domain 2 394 470 13
PF03764 Pfam IPR005517 Translation elongation factor EFG/EF2, domain IV 605 720 4
PF00679 Pfam IPR000640 Translation elongation factor EFG, V domain 724 810 7
PF14492 Pfam IPR009022 Elongation factor G, III-V domain 486 548 5
PF00009 Pfam Transcription factor, GTP-binding domain 18 344 16
SM00889 SMART IPR005517 Translation elongation factor EFG/EF2, domain IV 604 721 4
SM00838 SMART IPR000640 Translation elongation factor EFG, V domain 723 812 6
PS51722 Prosite Profiles IPR000795 Transcription factor, GTP-binding domain 17 253 17
PS00301 Prosite Patterns IPR031157 Tr-type G domain, conserved site 58 73 10
PTHR23115:SF187 HMMPANTHER 371 683 2
PTHR23115:SF187 HMMPANTHER 731 816 2
PTHR23115:SF187 HMMPANTHER 2 220 2
PTHR23115:SF187 HMMPANTHER 333 345 2
PTHR23115:SF187 HMMPANTHER 243 250 2
PTHR23115 HMMPANTHER 371 683 17
PTHR23115 HMMPANTHER 731 816 17
PTHR23115 HMMPANTHER 2 220 17
PTHR23115 HMMPANTHER 333 345 17
PTHR23115 HMMPANTHER 243 250 17
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 2 228 288
3.30.70.870 Gene3D Translation elongation factor EFG/EF2 486 560 7
2.40.30.10 Gene3D Phenol hydroxylase reductase 339 484 29
3.30.230.10 Gene3D IPR014721 Ribosomal protein S5 domain 2-type fold, subgroup 572 727 20
3.30.70.240 Gene3D IPR000640 Translation elongation factor EFG, V domain 728 828 7
1n0uA03 Gene3D 229 328 4
SSF54980 SuperFamily IPR009022 Elongation factor G, III-V domain 484 559 7
SSF54211 SuperFamily IPR020568 Ribosomal protein S5 domain 2-type fold 561 724 29
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 4 343 294
SSF50447 SuperFamily IPR009000 Translation protein, beta-barrel domain 344 481 24
SSF54980 SuperFamily IPR009022 Elongation factor G, III-V domain 726 840 7
PR00315 PRINTS IPR000795 Transcription factor, GTP-binding domain 117 128 15
PR00315 PRINTS IPR000795 Transcription factor, GTP-binding domain 65 73 15
PR00315 PRINTS IPR000795 Transcription factor, GTP-binding domain 21 34 15
PR00315 PRINTS IPR000795 Transcription factor, GTP-binding domain 101 111 15
PR00315 PRINTS IPR000795 Transcription factor, GTP-binding domain 153 162 15
TIGR00231 tigrfam IPR005225 Small GTP-binding protein domain 20 164 35

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.73 Da
Charge -1.50
Codon Adaptation Index 0.82
Isoelectric point 6.37
Molecular weight 93.23 kDa
Number of residues 842
Modifications

Protein Modifications

Term NameResidueCount
ubiquitinylated lysineK15, K42, K159, K253, K632 512
Annotation ExtensionEvidenceResidueReference
IDA PMID:26412298
mass spectrometry evidence K15 PMID:26412298
mass spectrometry evidence K42 PMID:26412298
mass spectrometry evidence K159 PMID:26412298
mass spectrometry evidence K253 PMID:26412298
mass spectrometry evidence K632 PMID:26412298
sumoylated lysine 173
Annotation ExtensionEvidenceResidueReference
IDA PMID:26404184
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for eft202 (SPCP31B10.07)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
152630.81during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
51682.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
63during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
7.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3422
conserved in vertebrates3397
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load gene that interact physically with SPCP31B10.07 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byulp2SUMO deconjugating cysteine peptidase Ulp2 (predicted) Affinity Capture-MSPMID:24818994
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
External References
Database Identifier Description
NBRP SPCP31B10.07 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPCP31B10.07 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPCP31B10.07 BioGRID Interaction Datasets
Expression Viewer SPCP31B10.07 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPCP31B10.07 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPCP31B10.07 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPCP31B10.07 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPCP31B10.07 Polyadenylation Viewer (Gullerova lab)
pombeTV SPCP31B10.07 Transcriptome Viewer (Bähler Lab)
GEO SPCP31B10.07 GEO profiles
PInt SPCP31B10.07 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPCP31B10.07 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPCP31B10.07 Fission yeast phenotypic data & analysis
Cyclebase SPCP31B10.07.1 Cell Cycle Data
SPD / RIKEN29/29C06Orfeome Localization Data
UniProtKB/SwissProtO14460Elongation factor 2
ModBaseO14460Database of comparative protein structure models
STRINGO14460Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_587863translation elongation factor 2 (EF-2) Eft2,B
RefSeq mRNANM_001022856972h- translation elongation factor 2 (EF-2) Eft2,B (eft202), mRNA
European Nucleotide ArchiveCU329670ENA EMBL mapping
European Nucleotide ArchiveCU329672ENA EMBL mapping
European Nucleotide ArchiveD83975ENA EMBL mapping
European Nucleotide ArchiveD83976ENA EMBL mapping
European Nucleotide ArchiveD89151ENA EMBL mapping
European Nucleotide ArchiveBAA13813ENA Protein Mapping
European Nucleotide ArchiveBAA23590ENA Protein Mapping
European Nucleotide ArchiveBAA23591ENA Protein Mapping
European Nucleotide ArchiveCAB52147ENA Protein Mapping
European Nucleotide ArchiveCAB58373ENA Protein Mapping
UniParcUPI0000129C7EUniProt Archive

Literature for eft202

Search: Europe PMC or PubMed

Release Version: PomBase:30_57 - 27 Jan 2016