Telomeres
The fission yeast complete genome sequence currently stops short of the telomeric repeats. See the Sequencing Status page for the current assembly status.
The most proximal anchored cosmids to each telomere are:
- Chromosome I left c212 (coordinates 1-29663)
- Cromosome I right c750 (5554844-5579133)
- Chromosome II left c1348 (1-39186)
- Chromosome II right pT2R1 (4500619-4539800)
- There are no telomere proximal clones for chromosome III as the unsequenced rDNA blocks occur inbetween the sequenced portion and the telomeres on both chromosome arms.
Details of all clones used for the assembly, and their order, length and overlap details is provided in a set of spreadsheets available from the S. pombe FTP site.
A contig extending the left arm of Chromosome II was sequenced by Sasaki et al. (PMID:18727152) and will be attached to the assembly in the next round of sequence changes. In the meantime, the contig can be viewed in the PomBase Genome Browser.
A set of small insert clones (FTP link; also see table) from a telomere plasmid library has been made available by Neal Sugawara. None can be assigned to a chromosome at present.
Clones included in the final sequence are available from the Wellcome Trust Sanger Institute; send email to archives@sanger.ac.uk
| Plasmid | Size of insert | Location of telomeric sequence | Comments | Vector | Laboratory | Funded by | Stage |
|---|---|---|---|---|---|---|---|
| pNSU28 | approx 1kb | Lies in pNSU21 | pUC19 | Hinxton | EC | finished | |
| pNSU31 | approx 1kb | Lies in pNSU21 | pUC19 | Hinxton | EC | finished | |
| pNSU68 | 423 bp | Internal | Contains 195bp of telomeric DNA and 123bp from the rDNA. | pMLC28 | Hinxton | EC | finished |
| pNSU71 | 15 kb | Terminal | pMLC28 | Hinxton | EC | finished | |
| pNSU64* | 6.9 kb | Terminal | pMLC28 | Hinxton | EC | finished | |
| pNSU70 | 7.1 kb | pMLC28 | Hinxton | EC | finished | ||
| pNSU77* | 12 kb | Internal | Fusion between telomere sequences (7.1kb) and rDNA sequences (4.9kb) | pMLC28 | Hinxton | EC | finished |
| pNSU21 | 7.9 kb | Terminal | pNSU21 and pNSU65 were isolated from the first and second library respectively | pMLC28 | Hinxton | EC | finished |
| pNSU65 | 8.1 kb | Terminal | pNSU21 and pNSU65 were isolated from the first and second library respectively | pMLC28 | Hinxton | EC | finished |
Table notes:
"Terminal" refers to the position adjacent to the vector sequences of pMLC12 where the blunt end (SmaI end) ligated to the telomeric sequences. "Internal" means that an S. pombe sequence intervenes between the telomere and vector sequences.