Cloning and sequence analysis of an ERG24 homolog from Schizosaccharomyces pombe.
The Schizosaccharomyces pombe (Sp) erg24 cDNA, encoding C-14 sterol reductase (erg24p), has been cloned and sequenced. The nucleotide sequence of Sp erg24 contains an open reading frame encoding a 424-amino-acid protein. The deduced aa sequence of Sp erg24 shows significant homology with Saccharomyces cerevisiae (Sc) Erg24p, as well as with other members of a larger gene family that includes yeast C-24(28) sterol reductase (Erg4p) and a vertebrate inner nuclear membrane protein, the lamin B receptor (LBR).
Smith S