Evolutionary diversification of the autophagy initiation complex: reduced Atg101 dependency and changes in Atg9 binding to Atg13.
Macroautophagy/autophagy is an evolutionarily conserved process through which cells degrade cytoplasmic substances via autophagosomes. During the initiation of autophagosome formation, the ULK/Atg1 complex serves as a scaffold that recruits and regulates downstream ATG/Atg proteins and ATG9/Atg9-containing vesicles. Despite the essential role of the ULK/Atg1 complex, its components have changed during evolution; the ULK complex in mammals consists of ULK1 (or ULK2), RB1CC1, ATG13, and ATG101, whereas the Atg1 complex in the yeast  Saccharomyces cerevisiae  lacks Atg101 but instead has Atg29 and Atg31 along with Atg17. In this study, we investigated how such changes have evolved. A BLAST analysis across the major eukaryotic clades revealed that  ATG101 , which is essential for autophagy in mammals, was lost in some Holomycota lineages after acquisition of  ATG29  and  ATG31  by their common ancestor. Additionally, the acquisition of a cap structure in Atg13 preceded the loss of  ATG101 . However, some Holomycota species have both  ATG101  and  ATG29-ATG31 , including  Aspergillus oryzae  and  Komagataella phaffii . Yeast two-hybrid assays showed that ATG101 is required for ATG13-ATG9 interaction in mammals but dispensable in  A. oryzae , probably because of a shift in the  Ao Atg9-binding site in  Ao Atg13. We found an additive effect between  atg101  and  atg31  deletions in starvation-induced autophagy in  K. phaffii . Furthermore, both  Kp Atg101 and  Kp Atg31 are involved in Atg1 complex assembly in  K. phaffii . These findings suggest that the reduced importance of Atg101 in the Atg13-Atg9 interaction and Atg1 complex assembly enabled the eventual loss of  ATG101  in some Holomycota species, including  S. cerevisiae .
Lai Z, Hama Y, Oku M, Zhang S, Sakai Y, Yamamoto H, Mizushima N