Effects of  rpl1001  Gene Deletion on Cell Division of Fission Yeast and Its Molecular Mechanism.
The  rpl1001  gene encodes 60S ribosomal protein L10, which is involved in intracellular protein synthesis and cell growth. However, it is not yet known whether it is involved in the regulation of cell mitosis dynamics. This study focuses on the growth, spore production, cell morphology, the dynamics of microtubules, chromosomes, actin, myosin, and mitochondria of fission yeast ( Schizosaccharomyces pombe ) to investigate the impact of  rpl1001  deletion on cell mitosis. RNA-Seq and bioinformatics analyses were also used to reveal key genes, such as  hsp16 ,  mfm1  and  isp3 , and proteasome pathways. The results showed that  rpl1001  deletion resulted in slow cell growth, abnormal spore production, altered cell morphology, and abnormal microtubule number and length during interphase. The cell dynamics of the  rpl1001Δ  strain showed that the formation of a monopolar spindle leads to abnormal chromosome segregation with increased rate of spindle elongation in anaphase of mitosis, decreased total time of division, prolonged formation time of actin and myosin loops, and increased expression of mitochondrial proteins. Analysis of the RNA-Seq sequencing results showed that the proteasome pathway, up-regulation of  isp3 , and down-regulation of  mfm1  and  mfm2  in the  rpl1001Δ  strain were the main factors underpinning the increased number of spore production. Also, in the  rpl1001Δ  strain, down-regulation of  dis1  caused the abnormal microtubule and chromosome dynamics, and down-regulation of  hsp16  and  pgk1  were the key genes affecting the delay of actin ring and myosin ring formation. This study reveals the effect and molecular mechanism of  rpl1001  gene deletion on cell division, which provides the scientific basis for further clarifying the function of the Rpl1001 protein in cell division.
Yu W, Yuan R, Liu M, Liu K, Ding X, Hou Y