Monitoring Local Chromatin Dynamics Regulated by SMC Complexes in Fission Yeast.
Structural maintenance of chromosomes (SMC) complexes organize genome architecture throughout the cell cycle. Condensin, a member of the SMC complex family, engages chromatin loci by embracing chromatin within its ring and constrains the dynamics of chromatin fiber to shape mitotic chromosomes. To understand how condensin constrains chromatin dynamics, it is crucial to monitor the mobility of chromatin fiber with high temporal and spatial resolution. Here, I describe a step-by-step protocol to record the dynamics of chromatin locus visualized using the LacO/LacI system in fission yeast, followed by the determination of its physical properties. This method is applicable not only for monitoring the dynamics of chromatin fiber but also for tracking various types of particles in living cells.
Kakui Y