The hermes transposon of Musca domestica is an efficient tool for the mutagenesis of Schizosaccharomyces pombe.
Currently, no transposon-based method for the mutagenesis of Schizosaccharomyces pombe exists. We have developed such a system based on the introduction of the hermes transposon from the housefly into S. pombe. This system efficiently disrupts open reading frames and allows the insertion sites to be readily identified.
Evertts AG, Plymire C, Craig NL, Levin HL