Rrp14 controls rRNA transcription via facilitating the translocation of Pol5 into the nucleolus.
Rrp14 is a conserved protein that plays an important role in rRNA processing and ribosomal biogenesis. In  Schizosaccharomyces pombe , the  rrp14  gene is split into  SPAC8C9.10 c  ( rrp14 ) and  SPBC947.07  ( rrp1402 ). Although the  SPAC8C9.10 c  gene is not essential for  S. pombe  survival, deletion of the gene causes the yeast cells to grow sick and to exhibit decreased rRNA transcription. We identified a novel Pol5 protein that physically interacts with the Rrp14 protein. Taking advantage of the Pil1 co-tethering assay, we found that Rrp14 facilitates the nucleolus translocation of Pol5, and the 7-RINAWN-12 motif of the Rrp14 protein is responsible for the interaction between Pol5 and Rrp14. Since deletion of the 7-RINAWN-12 motif affects rRNA transcription, we thus propose that Rrp14 affects rRNA transcription by facilitating the nucleolus translocation of Pol5.
Lin Z, Liu H, Chen H, Cao H, Liu X, Zhu H, Zhao L, Chen Z