Generation of temperature sensitive mutations with error-prone PCR in a gene encoding a component of the spindle pole body in fission yeast.
Temperature-sensitive (ts) mutants provide powerful tools for investigation of cellular functions of essential genes. We report here asimple procedure to generate ts mutations using error-prone PCR within  pcp1  that encodes aspindle pole body (SPB) component in  Schizosaccharomyces pombe . This manipulation is not restricted to  pcp1 , and can be suited to any essential genes involved in other processes.
Tang NH, Fong CS, Masuda H, Jourdain I, Yukawa M, Toda T