Proteomic analysis of meiosis and characterization of novel short open reading frames in the fission yeast  Schizosaccharomyces pombe .
Meiosis is the process by which haploid gametes are produced from diploid precursor cells. We used stable isotope labeling by amino acids in cell culture (SILAC) to characterize the meiotic proteome in the fission yeast  Schizosaccharomyces pombe . We compared relative levels of proteins extracted from cells harvested around meiosis I with those of meiosis II, and proteins from premeiotic S phase with the interval between meiotic divisions, when S phase is absent. Our proteome datasets revealed peptides corresponding to short open reading frames (sORFs) that have been previously identified by ribosome profiling as new translated regions. We verified expression of selected sORFs by Western blotting and analyzed the phenotype of deletion mutants. Our data provide a resource for studying meiosis that may help understand differences between meiosis I and meiosis II and how S phase is suppressed between the two meiotic divisions.
Huraiova B, Kanovits J, Polakova SB, Cipak L, Benko Z, Sevcovicova A, Anrather D, Ammerer G, Duncan CDS, Mata J, Gregan J