Construction of a New Drug and Agrochemical Candidates Screening Platform Utilizing Drug-Hypersensitive Fission Yeast to Discover Overlooked Natural Products.
Natural products exhibiting selective or preferential antifungal activity against fission yeast over budding yeast might contribute greatly to new discoveries in the life sciences and new drug and agrochemical development. However, it is difficult to discover new drug and agrochemical candidates in the fission yeast screening system due to its low drug sensitivity. In this study, we constructed a new antifungal drug and agrochemical candidate screening platform using a drug-hypersensitive fission yeast strain. We executed antifungal activity profiling of our natural compound and microbial libraries against drug-hypersensitive fission yeast and multidrug-sensitive budding yeast. Ultimately, we identified MS-347a as a promising agrochemical candidate due to its excellent activity against the rice blast fungus.
Kimishima A, Negami S, Honma S, Kawashima SA, Yashiroda Y, Fuji SI, Kojima H, Tokiwa T, Sugawara A, Ujie Y, Abe A, Chinen T, Yoshida M, Usui T, Asami Y