Generation and characterization of a temperature-sensitive mutant allele of the second largest subunit of RNA polymerase I in  Schizosaccharomyces pombe .
RNA polymerase I (Pol I) is a highly conserved complex that catalyzes the transcription of rRNA precursors in the nucleolus. In this study, we isolated a temperature-sensitive (ts) allele of Rpa2, the second largest subunit of Pol I in the fission yeast  Schizosaccharomyces pombe  . We found that  rpa2   ts  cells were severely defective in growth at temperatures above 32 °C. We also found that  rpa2   ts  cells showed aberrant chromosome segregation and an abnormal ring-like nuclear structure at the restrictive temperature. These findings suggest that Rpa2 is essential for faithful nuclear division and nuclear structural organization in  S. pombe  .
Ishida K, Tanaka K, Kawakami K