Optimization of the CRISPR/Cas9 system using  adh1  promoter derivatives in fission yeast.
The CRSIPR/Cas9 system has been applied to fission yeast, but there remain some rooms for improvement. Here we report that the weaker versions of the  adh1   +  promoter,  adh11  and  adh41  promoters, for the potentially cytotoxic Cas9 achieved highly efficient mutagenesis and gene deletion at the  ade6   +  locus. Employing a drug-selectable marker instead of conventional auxotrophic markers, our new vector system is compatible with a variety of experimental settings including prototrophic/auxotrophic strains and complete/minimal media.
Saito M, Nakaoka H, Hayashi A, Takaku H, Yamazaki H