A marker-free genome editing method in  S. pombe  using the  delitto perfetto  approach.
The fission yeast, like budding yeast, offer an easy manipulation of their genome, despite their distinct biology. Most tools available in budding yeast are also available in fission yeast in versions taking into account the features of each organism. The  delitto perfetto  is a powerful approach, initially developed in  S. cerevisiae  , for  in vivo  site-directed mutagenesis. Here, we present an adaptation of the approach to  S. pombe  manipulation and demonstrate its applicability for a rapid, marker-free and efficient  in vivo  site-directed mutagenesis and N-terminal tagging of nonessential genes in fission yeast.
Fréon K, Lambert SAE, Lobachev KS, Ait Saada A