methotrexate binding
Binding to methotrexate, an antineoplastic antimetabolite with immunosuppressant properties. It is an inhibitor of tetrahydrofolate reductase and prevents the formation of tetrahydrofolate, necessary for synthesis of thymidylate, an essential component of DNA.
GO:0031406
carboxylic acid binding
is_a
GO:1901363
heterocyclic compound binding
is_a