second spliceosomal transesterification activity
Catalysis of the second transesterification reaction of spliceosomal mRNA splicing. Ligation of the two exons occurs via a transesterification reaction where the free 3'-hydroxyl group of the 5' exon is the nucleophile attacking the 3' splice site. Non-expressed sequences are now detached from the exons. In cis splicing, the intron is in a lariat structure.
GO:0140098
catalytic activity, acting on RNA
is_a
3'-splice site cleavage, exon ligation
broad
lariat formation, 5'-splice site cleavage
related
SPAC4F8.12c