obsolete 2-methylthio-N-6-(cis-hydroxy)isopentenyl adenosine-tRNA biosynthesis (obsolete GO:0002195)
OBSOLETE. The chemical reactions and pathways involved in the biosynthesis of 2-methylthio-N-6-(cis-hydroxy)isopentenyl adenosine (ms2io6A), a modified nucleoside present in position 37 (adjacent to and 3' of the anticodon) of tRNAs.