type III site-specific deoxyribonuclease activity
Catalysis of the endonucleolytic cleavage of DNA to give double-stranded fragments with terminal 5'-phosphates. ATP hydrolysis is required. Cleavage is dependent on the presence of two copies of a specific recognition sequence in an inverse orientation in the DNA. Cleavage occurs at a specific distance from one of the recognition sites.
GO:0015666
restriction endodeoxyribonuclease activity
is_a
GO:0016888
DNA endonuclease activity, producing 5'-phosphomonoesters
is_a
type III restriction enzyme activity
exact