RNA polymerase II preinitiation complex assembly
The formation of a large multiprotein-DNA complex that self-assembles on gene promoter through the sequential recruitment of the general initiation factors that compose the preinitiation complex (PIC) (which may include TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and TFIIH complexes). The PIC engages RNA polymerase II on its DNA template strand and sparks polymerization of the first few RNA nucleotides of the nascent transcript, of which 8 are base-paired with the DNA template within a DNA bubble. PIC assembly may result in a pause step, which marks the end of the PIC assembly and may be followed by promoter clearance (exact synonym: promoter escape). For RNA polymerase II PIC assembly is preceded by the formation of a nucleosome-free region that allows the transcription machinery to access the promoter DNA.
GO:0070897
transcription preinitiation complex assembly
is_a
GO:0006367
transcription initiation at RNA polymerase II promoter
part_of
RNA polymerase II transcription PIC biosynthesis
exact
RNA polymerase II transcription PIC formation
exact
RNA polymerase II transcriptional preinitiation complex assembly
exact
RNA polymerase II transcriptional preinitiation complex formation
exact
SPCC553.11c SPAC23G3.09 SPAC15A10.02 SPCC1494.02c SPBC30D10.02 SPCC16C4.18c SPAC1002.04c SPAC16E8.16 SPAC2G11.14 SPCC1682.16 SPAC16A10.02 SPAC13F5.02c SPAC12G12.05c