separation of function mutant (silencing/chromosome segregation) ea1ec138d2671e21 swi6-sm1(unrecorded)[Not_assayed] Gar2 aa 1-163,Ura4 aa 1-39 cbaaa61496b71364 gar2::ura4+(Gar2_aa_1-163,Ura4_aa_1-39)[Not_assayed] Sequence position was annotated as G1038 in the paper, but it is G1039 (Manu) 3b7eef1018efec35 kms1-1(1039)[Not_assayed] nmt1-cig2+ aba22b8a34948b96 cig2+(unrecorded)[Overexpression] nmt? aba22b8a34948b96 cdc13+(wild_type)[Knockdown] kinase dead be9521cb5c4791ae rad3-D2249E(D2249E)[Not_assayed] rad11-D223Y(D223Y)[Wild_type] kinase-dead be9521cb5c4791ae rad3-D2249E(D2249E)[Not_assayed] poz1delta(unrecorded)[unknown_expression] promoter mutant fcd0b8c6ab5ccb12 gsa1--365--234((-365)-(-234))[Not_assayed] P cell genome 46c89f342f117ca5 mei3delta(unrecorded)[unknown_expression] chimeric protein f7e61f1bbab2a27b myo2-LT(myo2-myp2(1622-2104))[Wild_type] myp2delta(unrecorded)[unknown_expression] chimeric protein f7e61f1bbab2a27b myo2-ST(myo2-myp2(1923-2104))[Wild_type] myp2delta(unrecorded)[unknown_expression] chimeric protein f7e61f1bbab2a27b myo2-ST(myo2-myp2(1923-2104))[Wild_type] protease inactivated 2a8be2bb0239204b cnp3C-TEV(cnp3C-TEV)[Not_assayed] plo1-TEVAA341(plo1(1-341)-(ENLYFQGAS)-plo1(342-405)-(ENLYFQGAS)-plo1(406-683))[Wild_type] analogue sensitive a7c6118865e72409 cdk9-T120G(T120G)[Wild_type] analogue sensitive a7c6118865e72409 mcs6-L87G(L87G)[Wild_type] analogue sensitive a7c6118865e72409 lsk1-F353G(F353G)[Wild_type] phosphatase dead mutant 3472d1b25692f40c clp1-D257A(D257A)[Wild_type] analogue sensitive 8b5476ba0daf2d2d sty1-T97A(T97A)[Not_assayed] promoter mutant fe620cd046781e01 gst3--378--998((-998)-(-378))[Not_assayed] nmt1-cig2+ 5f9739dd2f7a875d cig2+(unrecorded)[Overexpression] deletion of cyclin destruction box 5f9739dd2f7a875d cdc13delta90(1-90)[Overexpression] rum1 nmt promoter 93c624d8e120755e rum1+(wild_type)[Overexpression] wee1-50(G850E)[Not_assayed] mik1delta(unrecorded)[unknown_expression] GDP-locked 05ebdc773cdd0a9d gtr1-S16N(S16N)[Not_assayed] analogue sensitive 8a66efe1aac64683 cdk9-T120G(T120G)[Not_assayed] ssu72-C13S(C13S)[Not_assayed] analogue sensitive 8a66efe1aac64683 cdk9-T120G(T120G)[Wild_type] analogue sensitive 8a66efe1aac64683 lsk1-F353G(F353G)[Wild_type] P81nmt-rgf1rgf2delta c3eceb1486a6bc53 rgf2delta(unrecorded)[unknown_expression] rgf1+(unrecorded)[Knockdown] nondegradable cyclin B ff759b3e7d7da717 cdc13delta1-67(1-67)[Wild_type] nondegradable cyclin B ff759b3e7d7da717 cdc13delta1-67(1-67)[Wild_type] cdc13+(wild_type)[Wild_type] Allele numbering PMID:15173383 are based on the Pignede sequence (off by 2) 44ae5ece389a1b0a cdc6-C1058S(C1058S)[Wild_type] Allele numbering PMID:15173383 are based on the Pignede sequence (off by 2) 44ae5ece389a1b0a cdc6-C1058A(C1058A)[Wild_type] Allele numbering PMID:15173383 are based on the Pignede sequence (off by 2) 44ae5ece389a1b0a cdc6-C1040A(C1040A)[Wild_type] Allele numbering PMID:15173383 are based on the Pignede sequence (off by 2) 44ae5ece389a1b0a cdc6-C1053A(C1053A)[Wild_type] Allele numbering PMID:15173383 are based on the Pignede sequence (off by 2) 44ae5ece389a1b0a cdc6-C1043A(C1043A)[Wild_type] deleted residues inferred from EcoRV-KpnI deleted region c5bcc1ff40b42c22 cdc27partial_delta(37-372)[Not_assayed] TEV cleavable db9b5a7b5f36c25c rec8(TEV)(rec8(1-361)-(ENLYFQGAS)-rec8(362-561))[Not_assayed] rec8(TEV)(rec8(1-361)-(ENLYFQGAS)-rec8(362-561))[Not_assayed] GDP-locked b793085c4da3e210 arf6-T52N-mNG(T52N)[Not_assayed] GDP-locked 07fb3e57038e972d gtr1-S16N(S16N)[Not_assayed] Another version of atf1-11M exists with T77A 57346d6578962342 atf1-11M-T77I(S2A,S4A,T77I,S140A,S152A,S172A,T204I,T216I,S226A,T249I,S438A)[Overexpression] phosphomimetic 57346d6578962342 atf1-10D(S2D,S4D,T77E,S140D,S152D,S172D,T204E,T216E,S226D,T249E)[Overexpression] Walker-B mutation 94ca468a0f015cd5 psm1-1B(E1161Q)[Not_assayed] Walker-B mutation 94ca468a0f015cd5 psm3-3B(E1126Q)[Not_assayed] psm1-1B(E1161Q)[Not_assayed] 608 first aminoacids deleted, most likely d30173fb3bf67d59 myo1-tail(2-608)[Wild_type] IQ domain removed d30173fb3bf67d59 myo1deltaIQ(726-784)[Wild_type] nmt1 promoter b3bc9cbfdcacc24c shk1-K415R(K415R)[Overexpression] tea1delta(unrecorded)[unknown_expression] ATPase-inactivating 711d04e89064d5c4 psm3-WA(K38I)[Not_assayed] ATPase-inactivating 711d04e89064d5c4 psm1-WA(K38I)[Not_assayed] promoter mutant 4ceda21f11a0f386 ggt1--156--754((-754)-(-156))[Not_assayed] promoter mutant 4ceda21f11a0f386 ggt1--156--421((-421)-(-156))[Not_assayed] promoter mutant 4ceda21f11a0f386 ggt1--421--754((-754)-(-421))[Not_assayed] separation of function mutant (silencing/chromosome segregation) 8ac46a804d130afe swi6-sm1(unrecorded)[Not_assayed] temperature sensitive edfee9c20ff8ca78 psc3-4T(unrecorded)[Not_assayed] cytoplasm localised c480fb02b530ca54 rga4delta(622-760)-blt1(rga4delta(622-760)-blt1)[Overexpression] TPPR from dis3 C-term 31b52625e666e444 sds21-TPPR(313-322,A312-TPPRKNKTGNSK)[Overexpression] phosphatase dead mutant d378014af8ea8779 clp1-D257A(D257A)[Wild_type] catalytic site mutant 961441fff5b656f5 eso1-D147N(D147N)[Wild_type] kinase dead 1d23a09d00d999d4 cki3-K44R(K44R)[Not_assayed] nuclear localization reduced 772e462e74088043 pfh1-m21(M21A)[Knockdown] analogue sensitive 6186ba197a53e798 cdk9-T120G(T120G)[Wild_type] ura4 inserted in ZF motif c3bdc1e547b6330a gaf1-ZF:ura4(unrecorded)[unknown_expression] temperature sensitive 96a3c95dd4918678 sds22-140-6(1-52)[Not_assayed] kinase dead 9ffcc61dd29b2c64 cki3-K44R(K44R)[Not_assayed] nondegradable cyclin B f8b9e68a8b938c8d cdc13delta1-67(1-67)[Wild_type] ATP binding site mutant 6eecc9d84084dc5c pnk1-K269R(K269R)[Not_assayed] phosphatase domain mutation 6eecc9d84084dc5c pnk1-D74A(D74A)[Not_assayed] drug efflux pathways dismantled (MDR-suppressed) d8d0ae0fabd341f8 nda3-TB101(Y50S)[Wild_type] deleted head domain 8505764defef1039 cut7+(wild_type)[Wild_type] cut7-ST(1-443)[Not_assayed] deleted head domain 8505764defef1039 cut7-ST(1-443)[Not_assayed] Constitutively active bfcb5a1106855fcd ppb1deltaC(unknown)[Not_assayed] ccr1delta(unrecorded)[unknown_expression] Constitutively active bfcb5a1106855fcd ppb1deltaC(unknown)[Not_assayed] weak overexpression c6ced0325cbf00af nmt81-CENP-A-(cnp1+)(unrecorded)[Overexpression] high overexpression c6ced0325cbf00af nmt1-CENP-A(cnp1+)(unrecorded)[Overexpression] medium overexpression c6ced0325cbf00af nmt41-CENPA(cnp1+)(unrecorded)[Overexpression] eisosome targeting domain 086fa578860a41d0 sle1delta298-636(298-636)[Not_assayed] nak1+ and orb6+ wild type product levels f4425b94136060a0 nak1-orb6(GFP-nak1-K39R-orb6)[Not_assayed] sid2-250(unknown)[Not_assayed] nak1+ and orb6+ wild type product levels f4425b94136060a0 nak1-orb6(GFP-nak1-K39R-orb6)[Not_assayed] cdc3-124(E43K)[Not_assayed] nak1+ and orb6+ wild type product levels f4425b94136060a0 nak1-orb6(GFP-nak1-K39R-orb6)[Not_assayed] mor2-786(A1673G)[Not_assayed] nak1+ and orb6+ wild type product levels f4425b94136060a0 nak1*-orb6(GFP-nak1-K39R-orb6)[Not_assayed] orb6+ wild type product level f4425b94136060a0 nak1-orb6(GFP-nak1-K39R-orb6)[Not_assayed] nak1-167(unrecorded)[Not_assayed] nak1+ wild type product level f4425b94136060a0 nak1-orb6(GFP-nak1-K39R-orb6)[Not_assayed] orb6-25(unknown)[Not_assayed] nak1+ and orb6+ wild type product levels f4425b94136060a0 nak1-orb6(GFP-nak1-K39R-orb6)[Not_assayed] cdc3-124(E43K)[Not_assayed] cdc16-116(unknown)[Not_assayed] nak1+ and orb6+ wild type product levels f4425b94136060a0 nak1-orb6(GFP-nak1-K39R-orb6)[Not_assayed] pmo25-35(T236A)[Not_assayed] nak1+ and orb6+ wild type product levels f4425b94136060a0 nak1-orb6(GFP-nak1-K39R-orb6)[Not_assayed] DHFR degron 4488533ca5f8fdc3 cdc23-tstd(fusion;C239Y,AA424::TEV,degron)[Not_assayed] TEV cleavable 4488533ca5f8fdc3 cdc23-S424::TEV(cdc23(1-424)-(ENLYFQGAS)-cdc23(425-593))[Not_assayed] analogue sensitive 2d3035d3cfa925ea sty1-T97A(T97A)[Not_assayed] AS inserted after initiator methionine 3e3589df2ec0053d cdc8-AS(M1-ASM)[Not_assayed] nmt1-cig2+ ab08277b7b3bc9c4 cig2+(unrecorded)[Overexpression] nmt1-cig2+ ab08277b7b3bc9c4 cig2+(unrecorded)[Overexpression] basic region deleted aefacb75b8af3f8a pcr1delta16-171(16-171)[Not_assayed] spp2+ with auxin-inducible degron tag| spp2+ with auxin-inducible degron tag a3ce872c46b27485 cdc20-ct1deltaN(1-1217,Y1520N,D1625G,L1726S,S1904P)[Not_assayed] spp2+(unrecorded)[Knockdown] pol1+(unrecorded)[Knockdown] pol1+ with auxin-inducible degron tag,spp2+ with auxin-inducible degron tag a3ce872c46b27485 spp2+(unrecorded)[Knockdown] pol1+(unrecorded)[Knockdown] dpb2+ with auxin-inducible degron tag a3ce872c46b27485 dpb2+(unrecorded)[Knockdown] cdc20+ with auxin-inducible degron tag a3ce872c46b27485 cdc20+(unrecorded)[Knockdown] Error in Supp material (mentions twice LiL, but first one should be LiS). Small, medium, and large insertions in the FCCH loop of Uba1 (LiL, LiM, and LiL) were generated using PCR. Insertions are between positions 198 and 199 in Uba1 are composed of the following amino acid sequences: 'TG' for LiL, 'GRSRGTG' for LiM, and 'GRSRGRSRGTG' for LiL. 285ddfe564bdf7e2 ptr3-LiL(E198-GRSRGRSRGTG)[Not_assayed] TCTAACG insertion at position 1267 (bp) of ORF causing c2cd1d8a4d29ba93 pap1-N424STOP(N424*)[Not_assayed] kinase dead 09a0a50064982418 gad8-K259R(K259R)[Not_assayed] 100x overexpression b0c3bd95702f2fcc nmt1:sad1+(unrecorded)[Overexpression] moderate b0c3bd95702f2fcc sad1+2micron(unrecorded)[Overexpression] high level of overexpression c23b5043b024b0a5 nmt1-xlf1.AA(T180A,S192A)[Overexpression] medium-level overexpression c23b5043b024b0a5 nmt41-xlf1.AA(T180A,S192A)[Overexpression] Consistently, the phenotype of cut9-T98 was indistinguishable from that of cut9-665 720a7afb1d87000c cut9-T98(unrecorded)[Not_assayed] nt AATCCCTTAATAGTATGC 062ae65578cd43ea rad26.a14(C510-NPLIVC)[Not_assayed] Kinase dead b7c7becd0bc7a254 ark1-K147R(K147R)[Overexpression] C239Y=cdc23-1E3, ts degron is combined with a temperature-sensitive allele 37237b530ab38bc9 cdc23-tstd(fusion;C239Y,AA424::TEV,degron)[Knockdown] nuclear excluded f5403fffd3ecaf81 pac1-AA(pac1-FRB-GFP)[Wild_type] promoter switch-off f5403fffd3ecaf81 dhp1+(unrecorded)[Knockdown] temperature-sensitive, loss of function a6e8deacb9e3b2bd pkd2-B42(F335S,S408P,N521D,M530V,S534G,Q543E,I555T,I571T,N675K,T676Y,D677*)[Not_assayed] nt 930-1102 duplicated; translated product has aa 1-271 plus 24 aa from different reading frame e976a54955ad43a4 cdc27-R5(T1101-CATAAAGAAAAGGAGCCTCTCTTGCCAAAGGAGGAGAAGTTGTCTGAGCAAGCAAAGAGAGAGCGCGATGATCTGAAAAATATTATGCAGCTAGAAGATGAATCAGTATCTACCACTAGCGTTCACGATTCTGAAGATGATAATTTAGATTCTAATAATTTCCAATTGGAAAT)[Wild_type] nt 968-1025 duplicated; translated product has aa 1-245 plus 5 aa from different reading frame e976a54955ad43a4 cdc27-R40(G1025-TTGTCTGAGCAAGCAAAGAGAGAGCGCGATGATCTGAAAAATATTATGCAGCTAGAAG)[Wild_type] nt 813-828 duplicated; translated product has aa 1-179 plus 2 aa from different reading frame e976a54955ad43a4 cdc27-R3(A827-ATCGATAAGACTGACA)[Wild_type] nt C inserted after 782; translated product has aa 1-164 plus 12 aa from different reading frame e976a54955ad43a4 cdc27-R22(C782-C)[Wild_type] nt 807-828 duplicated; translated product has aa 1-179 plus 4 aa from different reading frame e976a54955ad43a4 cdc27-R39(A837-TCGACAATCGATAAGACTGACA)[Wild_type] nt 694-814 duplicated; translated product has aa 1-175 plus 2 aa from different reading frame e976a54955ad43a4 cdc27-R25(C814-GTTGTTTGACATTCGATCCTTCCGTCTTCGGAAGACTAACTTATTGTAGCCTCGTGTTTTGAAAAAGGCACCTTCAACTCATTCCCCCCAATTATCTGTTCCCTCAAAGACATCGACAATC)[Wild_type] promoter mutant aa227d6105947e03 gst1--151--1088((-1088)-(-151))[Not_assayed] promoter mutant aa227d6105947e03 gst1--770--1088((-1088)-(-770))[Not_assayed] half N-terminal RRM domain deleted 9f2a88f45c75895b msa1-DM2(2-102)[Not_assayed] half C-terminal RRM domain deleted 9f2a88f45c75895b msa1-DM3(487-533)[Not_assayed] cold sensitive d21f20d1d014ccba top2-250(unrecorded)[Not_assayed] non-hydrolyzable ATP fa8f1b63230268b1 smc5-E995Q(E995Q)[Not_assayed] nse4-L68C(L68C)[Not_assayed] non-hydrolyzable ATP fa8f1b63230268b1 smc5-E995Q(E995Q)[Not_assayed] inserted IMRRYR at C-term 8acb880886aa8d18 sos7-+6(K264-IMRRYR)[Wild_type] kinase dead 9a10046246e64418 gad8-K259R(K259R)[Not_assayed] nmt-promoter, thiamine repressible 50db88fdd925afa8 spg1+(wild_type)[Knockdown] Bioneer library, incomplete deletion 3a3a0d9e76ed714d atg43-1(atg43::kanMX4(nt49-203))[unknown_expression] reported as P611S in PMID:15546915 8702be0070bbe887 esh2-1(P612S)[Not_assayed] reported as P611S in PMID:15546915 8702be0070bbe887 rho1+(unrecorded)[Overexpression] esh2-1(P612S)[Not_assayed] reported as P611S in PMID:15546915 8702be0070bbe887 cdc42+(unrecorded)[Overexpression] esh2-1(P612S)[Not_assayed] Walker-B mutation 74dade3ace393390 psm1-1B(E1161Q)[Not_assayed] Walker-B mutation 74dade3ace393390 psm3-3B(E1126Q)[Not_assayed] psm1-1B(E1161Q)[Not_assayed] description refers to position within each CTD repeat 8481a1a5bf90b23e spt5-(T1A)7(T1A_mutations_within_the_CTR)[Not_assayed] description refers to position within each CTD repeat 8481a1a5bf90b23e spt5(T1E)7(T1E_mutations_within_the_CTR)[Not_assayed] loss of function e18451a565bca640 par1delta(unrecorded)[unknown_expression] spg1-106(unknown)[Not_assayed] nucleotide U1133C d86816507d979037 nda2noD(V348A)[Wild_type] Gar2 aa 1-163,Ura4 aa 1-39 ab7d71d62a2030e3 gar2::ura4+(Gar2_aa_1-163,Ura4_aa_1-39)[unknown_expression] kinase dead 819935350ed7d37f gad8-K259R(K259R)[Not_assayed] duplication of codons 345 to 363 a09d826a70b1a2d4 git7-93(S345-SYTESNGTALSTNWKDVKS)[Not_assayed] mes1 prevents meiosis II ae5f72244ad85ad1 pkl1delta(unrecorded)[unknown_expression] cut7delta(unrecorded)[unknown_expression] lumenal + TM domain (cytoplasmic domain deleted) 60b3b61056143fa0 cnx1-delta524-560(524-560)[Not_assayed] conditional depletion f0afc6fe7b0b439a cnd3+(unrecorded)[Knockdown] conditional depletion f0afc6fe7b0b439a cut14+(unrecorded)[Knockdown] Nse6 fragment used as lateral cortical region marker 88d2e1acdc9993bc cor-C(unrecorded)[Not_assayed] Increased cortical tubular ER dynamics c605477efc21b69a sle1delta(unrecorded)[unknown_expression] rtn1delta(unrecorded)[unknown_expression] Increased cortical tubular ER dynamics c605477efc21b69a sle1delta(unrecorded)[unknown_expression] sey1delta(unrecorded)[unknown_expression] Increased cortical tubular ER dynamics c605477efc21b69a yop1delta(unrecorded)[unknown_expression] tts1delta(unrecorded)[unknown_expression] sle1delta(unrecorded)[unknown_expression] nt -1790 to -1250 replaced with ura4+ fd7d19b1aed14f7c nc-tco1-Ldelta::ura4+(-395-+146)[Not_assayed] consensus H added to finger 9 bfc8cbcebd1237c6 sfc2-G289H(G289H)[Not_assayed] identity established in PMID:25103238 36f40baed62fc60c cut11-6(T498I)[Not_assayed] identity established in PMID:25103238 36f40baed62fc60c cut11-2(C525R)[Not_assayed] hypomorphic Med31 allele from Dicer G0 suppressor screen 93e1b181a0463890 dcr1delta(unrecorded)[unknown_expression] med31-ins(F23-LVRIC)[Not_assayed] hypomorphic Med31 allele from Dicer G0 suppressor screen 93e1b181a0463890 med31-ins(F23-LVRIC)[Not_assayed] identity established in PMID:25103238 7f17aec8a060844d cut11-6(T498I)[Not_assayed] SH3 and SAM domains deleted 6286f8354d3d54ef pob1deltaN(1-537)[Wild_type] PH domain deleted 6286f8354d3d54ef pob1deltaC(539-871)[Wild_type] motor domain 564d70e29944c82f tea2-M(1-123,476-628)[Not_assayed] motor domain + neck linker 564d70e29944c82f tea2delta477-628(477-628)[Not_assayed] linker 564d70e29944c82f tea2(2-122)(1,123-628)[Not_assayed] byr4::ura4+ a2468b284d810cf8 byr4delta(unrecorded)[unknown_expression] mob1-R4(unknown)[Not_assayed] G2-arrested a2468b284d810cf8 spg1+(wild_type)[Overexpression] the pho8 deletion has decreased alkaline phosphatase activity in high zinc conditions 2c0b3f9b052bea9e pho2delta(unrecorded)[unknown_expression] pho8delta(unrecorded)[unknown_expression] zinc binding mutant, site1 2c0b3f9b052bea9e pho8-m1(D311A,H315A)[Null] zinc binding mutant , site2 2c0b3f9b052bea9e pho8-m2(D352A,H353A)[Null] Q28R,Q176R are unintentional 7d2c05ef7277f909 ark1-as2(L166A,Q28R,Q176R)[Not_assayed] kinase dead d3c28a9773ee0a38 cdk8-D158A(D158A)[Wild_type] alg10 supressor mutation 661292025a8cdee8 alg10-183-stop(183-stop)[Not_assayed] ghs1delta(unrecorded)[unknown_expression] worse growth than "healthy" gls1delta 661292025a8cdee8 ghs1delta(unrecorded)[unknown_expression] worse growth than "healthy" gls1delta 661292025a8cdee8 alg10delta(unrecorded)[unknown_expression] ghs1delta(unrecorded)[unknown_expression] (stress-independent Sty1 activation f938aca48cd3f966 pyp2delta(unrecorded)[unknown_expression] pyp1delta(unrecorded)[unknown_expression] wis1-DD(S469D,T473D)[Not_assayed] sty1-T97A(T97A)[Not_assayed] analogue sensitive f938aca48cd3f966 sty1-T97A(T97A)[Not_assayed] protease cleavable e13e49a2df6b7722 plo1-TEVAA341(plo1(1-341)-(ENLYFQGAS)-plo1(342-405)-(ENLYFQGAS)-plo1(406-683))[Wild_type] deletion of cyclin destruction box 5ef3337d730491a3 cdc13delta90(1-90)[Overexpression] kinase dead 1f7c9dd717c9f378 gad8-K259R(K259R)[Not_assayed] ectopically localized, phosphomimic b015a7efb76f7cb7 TetR-spc7(1-666)-9TE(TetR-667-1364,T257E,T338E,T366E,T395E,T422E,T453E,T507E,T529E,T552E)[Not_assayed] ectopically localized, phosphomimic b015a7efb76f7cb7 bub3delta(unrecorded)[unknown_expression] TetR-D(1-302)Mph1(TetR-D(1-302))[Not_assayed] TetR-spc7(1-666)-9TE(TetR-667-1364,T257E,T338E,T366E,T395E,T422E,T453E,T507E,T529E,T552E)[Not_assayed] another atf1-11M exists with T77I 0b6d5503e51a6f05 atf1-11M-T77A(S2A,S4A,T77A,S140A,S152A,S172A,T204I,T216I,S226A,T249I,S438A)[Not_assayed] both RxxS phosphorylation sites disrupted f07cd572b2d0adcf rps602-S235A,S236A(S235A,S236A)[Not_assayed] rps601delta(unrecorded)[unknown_expression] C-terminal truncation 2a809a0d2e35ee91 spo5-N(225-297)[Wild_type] description based on restriction site 61076bdb7aff52c4 ksg1delta409-592(409-592)[Not_assayed] +5 seb1 binding sites 63cd6d753b3b6617 nc-tgp-+5_seb1_bs(G521T,T543A,A554T,C569G,C578G)[Knockdown] monoubiquitin ee139b613d53efbe ubi4-1(74-382)[Not_assayed]