A microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging.
We describe a microfluidic device designed specifically for the reversible immobilisation of  Schizosaccharomyces pombe  (Fission Yeast) cells to facilitate live cell super-resolution microscopy. Photo-Activation Localisation Microscopy (PALM) is used to create detailed super-resolution images within living cells with a modal accuracy of >25 nm in the lateral dimensions. The novel flow design captures and holds cells in a well-defined array with minimal effect on the normal growth kinetics. Cells are held over several hours and can continue to grow and divide within the device during fluorescence imaging.
Bell L, Seshia A, Lando D, Laue E, Palayret M, Lee SF, Klenerman D