Overcoming the problems associated with poor spectra quality of the protein kinase Byr2 using residual dipolar couplings.
For the Ras-binding domain of the protein kinase Byr2, only a limited number of NOE contacts could be initially assigned unambiguously, as the quality of the NOESY spectra was too poor. However, the use of residual (1)H-(15)N dipolar couplings in the beginning of the structure determination process allows to overcome this problem. We used a three-step recipe for this procedure. A previously unknown structure could be calculated reasonably well with only a limited number of unambiguously assigned NOE contacts.
Gronwald W, Brunner E, Huber F, Wenzler M, Herrmann C, Kalbitzer HR