Identification and characterization of  Schizosaccharomyces pombe  splicing mutants.
Pre-mRNA splicing is carried out by the spliceosome, a dynamic complex of five small nuclear ribonucleoprotein particles (snRNPs). Several genetic screens have been conducted in  Schizosaccharomyces pombe  to identify pre-mRNA splicing mutants and spliceosome components  .  However, some pre-mRNA splicing mutants have yet to be assigned to a gene and in certain cases, the mutations within genes have not been identified and phenotypes compared. Here, we have identified new mutations in the U4/U6.U5 tri-snRNP component  dim1  and assigned  prp6  and  prp7  mutants to  snu13  and  brl1  , respectively, revealing roles for these factors in pre-mRNA splicing.
Nicholas LL, Suo F, Hanna SM, Du LL, Gould KL