Changes in phosphoprotein pattern in Schizosaccharomyces pombe.
A variety of evidence suggests that protein phosphorylation (pp) may be important in cell-cycle control. Phosphorylated proteins from S. pombe have been examined for phosphorylation changes under several conditions: known triggers of the division control (low nitrogen, low phosphate), cell size mutants (WEE1 and CDC2 alleles) and cell cycle mutants (CDC2, CDC10, CDC17, CDC25 alleles). Three major phosphorylated proteins (pp38, pp45 and pp54) showed the greatest response to nutritional shifts. The changes in the phosphorylated states of these proteins correlated with growth rate. Some phosphorylations (e.g. pp53) occurred transiently following a stimulus to cell division suggesting a possible involvement with the division mechanism. An allele-specific alteration of charge was noted for pp45 suggesting that this protein is the product of the CDC2 gene. The wee1-6 phosphoprotein pattern is similar to wild-type indicating that this mutant cell line accurately senses its nutritional environment and that the mutation likely affects the transfer of this information to the division control. Cells blocked by various temperature-sensitive cell cycle mutants did not show an alteration of phosphoprotein pattern.
Querengesser LD, Young PG