The PPIP5K Family Member Asp1 Controls Inorganic Polyphosphate Metabolism in  S. pombe .
Inorganic polyphosphate (polyP) which is ubiquitously present in both prokaryotic and eukaryotic cells, consists of up to hundreds of orthophosphate residues linked by phosphoanhydride bonds. The biological role of this polymer is manifold and diverse and in fungi ranges from cell cycle control, phosphate homeostasis and virulence to post-translational protein modification. Control of polyP metabolism has been studied extensively in the budding yeast  Saccharomyces cerevisiae.  In this yeast, a specific class of inositol pyrophosphates (IPPs), named IP 7 , made by the IP6K family member Kcs1 regulate polyP synthesis by associating with the SPX domains of the vacuolar transporter chaperone (VTC) complex. To assess if this type of regulation was evolutionarily conserved, we determined the elements regulating polyP generation in the distantly related fission yeast  Schizosaccharomyces pombe . Here, the VTC machinery is also essential for polyP generation. However, and in contrast to  S. cerevisiae , a different IPP class generated by the bifunctional PPIP5K family member Asp1 control polyP metabolism. The analysis of Asp1 variant  S. pombe  strains revealed that cellular polyP levels directly correlate with Asp1-made IP 8  levels, demonstrating a dose-dependent regulation. Thus, while the mechanism of polyP synthesis in yeasts is conserved, the IPP player regulating polyP metabolism is diverse.
Pascual-Ortiz M, Walla E, Fleig U, Saiardi A