Live Imaging of Fission Yeast Single-Cell Lineages Using a Microfluidic Device.
Mother machine (MM) is a microfluidic device originally developed for long-term live imaging of Escherichia coli bacterial cells under a microscope. The simple yet sophisticated design has enabled microbiologists to track multiple single-cell lineages cultured under highly controlled external environments. Here, I describe how to fabricate a fission yeast version of MM with photolithography and soft lithography. Procedures for setting up the microfluidic device for long-term live microscopy are also explained.
Nakaoka H