Tandem affinity purification protocol for isolation of protein complexes from  Schizosaccharomyces pombe .
Many cellular processes require the activities of complex molecular machines composed of several protein subunits. Insights into these systems can be gained by isolation of protein complexes followed by  in vitro  analyses determining the identity, posttranslational modifications, and interactions among proteins. Here, we present a protocol for tandem affinity purification (TAP) of protein complexes from the fission yeast  Schizosaccharomyces pombe . The protocol employs cells expressing C-terminally TAP-tagged proteins and is suitable for the analysis of purified proteins by mass spectrometry. For complete information on the use and execution of this protocol, please refer to Cipakova et al. (2019).
Cipak L, Selicky T, Jurcik J, Cipakova I, Osadska M, Lukacova V, Barath P, Gregan J