Characterization of temperature-sensitive alleles of  Schizosaccharomyces pombe  septation initiation network components.
The  Schizosaccharomyces pombe  septation initiation network (SIN) promotes cytokinesis and septation. Comprised of a protein kinase cascade triggered by activation of a small GTPase and inhibited by a two-component GAP that localize to the spindle pole bodies in a cell cycle specific manner. Here, we characterized temperature-sensitive mutants isolated in the 1990s in four SIN components. We determined the mutations within each  cdc14  ,  cdc16  ,  sid1 ,  and  sid2  mutant allele and analyzed their growth at different temperatures compared with known mutant alleles. The new mutants described here expand the toolkit for studying SIN signaling.
Turner LA, Willet AH, Gould KL