Construction of vectors and a genomic library for use with his3-deficient strains of Schizosaccharomyces pombe.
The construction of vectors for use in Schizosaccharomyces pombe using the his3+ gene as a selectable marker is described. In addition, we report the construction of a genomic library in a his3(+)-containing shuttle vector to facilitate the cloning of genes by complementation of mutant function in strains defective for His3 activity.
Ohi R, Feoktistova A, Gould KL