Catalase T-Deficient Fission Yeast Meiocytes Show Resistance to Ionizing Radiation.
Environmental stress, reactive oxygen species (ROS), or ionizing radiation (IR) can induce adverse effects in organisms and their cells, including mutations and premature aging. DNA damage and its faulty repair can lead to cell death or promote cancer through the accumulation of mutations. Misrepair in germ cells is particularly dangerous as it may lead to alterations in developmental programs and genetic disease in the offspring. DNA damage pathways and radical defense mechanisms mediate resistance to genotoxic stresses. Here, we investigated, in the fission yeast  Schizosaccharomyces pombe , the role of the H 2 O 2 -detoxifying enzyme cytosolic catalase T (Ctt1) and the Fe 2+ /Mn 2+  symporter Pcl1 in protecting meiotic chromosome dynamics and gamete formation from radicals generated by ROS and IR. We found that wild-type and  pcl1 -deficient cells respond similarly to X ray doses of up to 300 Gy, while  ctt1 ∆ meiocytes showed a moderate sensitivity to IR but a hypersensitivity to hydrogen peroxide with cells dying at >0.4 mM H 2 O 2 . Meiocytes deficient for  pcl1 , on the other hand, showed a resistance to hydrogen peroxide similar to that of the wild type, surviving doses >40 mM. In all, it appears that in the absence of the main H 2 O 2 -detoxifying pathway  S. pombe  meiocytes are able to survive significant doses of IR-induced radicals.
Muhtadi R, Lorenz A, Mpaulo SJ, Siebenwirth C, Scherthan H