Structure and function of the  S. pombe  III-IV-cyt  c  supercomplex.
The respiratory chain in aerobic organisms is composed of a number of membrane-bound protein complexes that link electron transfer to proton translocation across the membrane. In mitochondria, the final electron acceptor, complex IV (CIV), receives electrons from dimeric complex III (CIII 2 ), via a mobile electron carrier, cytochrome  c . In the present study, we isolated the CIII 2 CIV supercomplex from the fission yeast  Schizosaccharomyces pombe  and determined its structure with bound cyt.  c  using single-particle electron cryomicroscopy. A respiratory supercomplex factor 2 was found to be bound at CIV distally positioned in the supercomplex. In addition to the redox-active metal sites, we found a metal ion, presumably Zn 2+ , coordinated in the CIII subunit Cor1, which is encoded by the same gene ( qcr  1 ) as the mitochondrial-processing peptidase subunit β. Our data show that the isolated CIII 2 CIV supercomplex displays proteolytic activity suggesting a dual role of CIII 2  in  S. pombe . As in the supercomplex from  S. cerevisiae , subunit Cox5 of CIV faces towards one CIII monomer, but in  S. pombe,  the two complexes are rotated relative to each other by ~45°. This orientation yields equal distances between the cyt.  c  binding sites at CIV and at each of the two CIII monomers. The structure shows cyt.  c  bound at four positions, but only along one of the two symmetrical branches. Overall, this combined structural and functional study reveals the integration of peptidase activity with the CIII 2  respiratory system and indicates a two-dimensional cyt.  c  diffusion mechanism within the CIII 2 -CIV supercomplex.
Moe A, Dimogkioka AR, Rapaport D, Öjemyr LN, Brzezinski P