Characterization and comparison of temperature-sensitive  Schizosaccharomyces pombe  mutants of the septation initiation network scaffolds, Cdc11 and Sid4.
The  Schizosaccharomyces pombe  septation initiation network (SIN) is required for cytokinesis and septation. The SIN includes a protein kinase cascade that is assembled at spindle pole bodies (SPBs) in a cell cycle specific manner on a scaffold consisting of Cdc11 , related to human centriolin, and the a-helical protein Sid4 . Here, we characterized temperature-sensitive  cdc11  and  sid4  mutants isolated in the 1990s. We determined the mutations within each allele, examined their phenotypes, and analyzed their growth compared with previously characterized mutant alleles. The new mutants described here expand the toolkit for studying how the SIN assembles at SPBs.
Turner LA, Fletcher AB, Willet AH, Gould KL