Cytoophidia Influence Cell Cycle and Size in  Schizosaccharomyces pombe .
Cytidine triphosphate synthase (CTPS) forms cytoophidia in all three domains of life. Here we focus on the function of cytoophidia in cell proliferation using  Schizosaccharomyces pombe  as a model system. We find that converting His 359  of CTPS into Ala 359  leads to cytoophidium disassembly. By reducing the level of CTPS protein or specific mutation, the loss of cytoophidia prolongs the G2 phase and expands cell size. In addition, the loss-filament mutant of CTPS leads to a decrease in the expression of genes related to G2/M transition and cell growth, including histone chaperone  slm9 . The overexpression of  slm9  alleviates the G2 phase elongation and cell size enlargement induced by CTPS loss-filament mutants. Overall, our results connect cytoophidia with cell cycle and cell size control in  Schizosaccharomyces pombe .
Deng R, Li YL, Liu JL