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protein coding gene - och1 (SPAC1006.05c) - Golgi alpha-1,6-mannosyltransferase Och1

Gene summary

Standard name
och1
Systematic ID
SPAC1006.05c
Product
Golgi alpha-1,6-mannosyltransferase Och1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9UTR6
ORFeome ID
17/17C05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 5078289..5080521 reverse strand

Annotation

GO biological process

GO:0000032 - cell wall mannoprotein biosynthetic process

References:

GO:0009101 - glycoprotein biosynthetic process

References:

GO:0006487 - protein N-linked glycosylation

References:

GO cellular component

GO:0005789 - endoplasmic reticulum membrane

References:

GO:0005794 - Golgi apparatus

References:

GO:0140497 - mannan polymerase II complex

References:

GO molecular function

GO:0000009 - alpha-1,6-mannosyltransferase activity

References:

GO:0033164 - initiation-specific glycolipid 1,6-alpha-mannosyltransferase activity

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GO:0000030 - mannosyltransferase activity

References:

Modification

MOD:00006 - N-glycosylated residue

References:

Multi-locus phenotype

FYPO:0006277 - abnormal N-linked glycoprotein glycan structure

References:

Genotypes:

FYPO:0002447 - abnormal protein N-linked glycosylation during vegetative growth

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000118 - multiseptate vegetative cell

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0001120 - pear-shaped vegetative cell

References:

Genotypes:

FYPO:0000106 - sensitive to hygromycin B

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

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Genotypes:

Qualitative gene expression

PomGeneEx:0000023 - protein level constant

References:

PomGeneEx:0000016 - RNA level constant

References:

PomGeneEx:0000011 - RNA level increased

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PomGeneEx:0000013 - RNA level unchanged

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0002447 - abnormal protein N-linked glycosylation during vegetative growth

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0007488 - decreased protein localization to Golgi apparatus, with protein mislocalized to vacuole

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0005760 - increased cell-cell adhesion

References:

Genotypes:

FYPO:0000224 - lemon-shaped cell

References:

Genotypes:

FYPO:0000672 - normal cell morphology

References:

Genotypes:

FYPO:0005761 - normal protein O-linked glycosylation during vegetative growth

References:

Genotypes:

FYPO:0000946 - normal volume spheroid vegetative cell

References:

Genotypes:

FYPO:0000094 - sensitive to benomyl

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0001188 - sensitive to Calcofluor White

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0000106 - sensitive to hygromycin B

References:

Genotypes:

FYPO:0000107 - sensitive to latrunculin A

References:

Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0003710 - swollen pear-shaped vegetative cell

References:

Genotypes:

FYPO:0001491 - viable vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011064 - conserved in fungi

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF04488Gly_transf_sugGlycoTrfase_DXD_sugar-bd_CSPFAM
SSF53448Nucleotide-diphospho-sugar transferasesNucleotide-diphossugar_transSUPERFAMILY
G3DSA:3.90.550.20GENE3D
PTHR31834INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASEOch1-likePANTHER

Orthologs

References / Literature

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:19844703 - N- and O-linked oligosaccharides completely lack galactose residues in the gms1och1 mutant of Schizosaccharomyces pombe.
Ohashi T et al. Appl Microbiol Biotechnol 2010 Mar;86(1):263-72
PMID:32650974 - SpMnn9p and SpAnp1p form a protein complex involved in mannan synthesis in the fission yeast Schizosaccharomyces pombe.
Ohashi T et al. J Biosci Bioeng 2020 Oct;130(4):335-340
PMID:11231017 - Schizosaccharomyces pombe och1(+) encodes alpha-1,6-mannosyltransferase that is involved in outer chain elongation of N-linked oligosaccharides.
Yoko-o T et al. FEBS Lett 2001 Jan 26;489(1):75-80
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:20854854 - Production of heterologous glycoproteins by a glycosylation-defective alg3och1 mutant of Schizosaccharomyces pombe.
Ohashi T et al. J Biotechnol 2010 Nov;150(3):348-56
PMID:21098516 - Structural analysis of α1,3-linked galactose-containing oligosaccharides in Schizosaccharomyces pombe mutants harboring single and multiple α-galactosyltransferase genes disruptions.
Ohashi T et al. Glycobiology 2011 Mar;21(3):340-51
PMID:22988247 - Identification of novel α1,3-galactosyltransferase and elimination of α-galactose-containing glycans by disruption of multiple α-galactosyltransferase genes in Schizosaccharomyces pombe.
Ohashi T et al. J Biol Chem 2012 Nov 09;287(46):38866-75
PMID:19202278 - The och1 mutant of Schizosaccharomyces pombe produces galactosylated core structures of N-linked oligosaccharides.
Ohashi T et al. Biosci Biotechnol Biochem 2009 Feb;73(2):407-14
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:12784644 - Salt stress induces the expression of Schizosaccharomyces pombe och1+, which encodes an initiation-specific alpha-1,6-mannosyltransferase for N-linked outer chain synthesis of cell wall mannoproteins.
Yamamoto K et al. Biosci Biotechnol Biochem 2003 Apr;67(4):927-9
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:27168121 - Discovery of genes involved in mitosis, cell division, cell wall integrity and chromosome segregation through construction of Schizosaccharomyces pombe deletion strains.
Chen JS et al. Yeast 2016 Sep;33(9):507-17
PMID:32918581 - Golgi localization of glycosyltransferases requires Gpp74p in Schizosaccharomyces pombe.
Ohashi T et al. Appl Microbiol Biotechnol 2020 Oct;104(20):8897-8909
PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.
Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663
PMID:33536395 - The fission yeast gmn2 + gene encodes an ERD1 homologue of Saccharomyces cerevisiae required for protein glycosylation and retention of luminal endoplasmic reticulum proteins.
Tanaka N et al. J Gen Appl Microbiol 2021 Jun 03;67(2):67-76
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:34805795 - The fission yeast FLCN/FNIP complex augments TORC1 repression or activation in response to amino acid (AA) availability.
Calvo IA et al. iScience 2021 Nov 19;24(11):103338
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
PMID:34738170 - Overexpression of cell-wall GPI-anchored proteins restores cell growth of N-glycosylation-defective och1 mutants in Schizosaccharomyces pombe.
Fukunaga T et al. Appl Microbiol Biotechnol 2021 Dec;105(23):8771-8781
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323