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protein coding gene - rgf2 (SPAC1006.06) - RhoGEF for Rho1 Rgf2

Gene summary

Standard name
rgf2
Systematic ID
SPAC1006.06
Product
RhoGEF for Rho1 Rgf2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9UTR5
ORFeome ID
30/30E10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 5080718..5085055 forward strand

Annotation

GO biological process

GO:0009272 - fungal-type cell wall biogenesis

References:

GO:0035556 - intracellular signal transduction

References:

GO:0140748 - positive regulation of regulation of ascospore wall (1->3)-beta-D-glucan biosynthetic process

References:

GO cellular component

GO:0051285 - cell cortex of cell tip

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GO:0051286 - cell tip

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GO:0005829 - cytosol

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GO:0000935 - division septum

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GO:0005632 - inner layer of spore wall

References:

GO molecular function

GO:0005085 - guanyl-nucleotide exchange factor activity

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Modification

MOD:00595 - monomannosylated residue

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MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00696 - phosphorylated residue

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Multi-locus phenotype

FYPO:0000175 - abnormal ascospore wall assembly

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Genotypes:

FYPO:0003440 - cell lysis during cytokinesis

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Genotypes:

FYPO:0003535 - decreased bipolar index

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Genotypes:

FYPO:0007910 - increased vegetative cell shrinkage

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0007829 - premature protein localization to cell division site

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Genotypes:

FYPO:0000105 - sensitive to cyclosporin A

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Protein features

PBO:0111759 - pleckstrin homology domain

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000011 - RNA level increased

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PomGeneEx:0000014 - RNA present

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000175 - abnormal ascospore wall assembly

References:

Genotypes:

FYPO:0007905 - abnormal beta-glucan synthesis

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Genotypes:

FYPO:0000351 - abnormal cell wall morphology during vegetative growth

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Genotypes:

FYPO:0000121 - abnormal sporulation

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Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

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Genotypes:

FYPO:0001968 - increased 1,3-beta-D-glucan synthase activity

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Genotypes:

FYPO:0007911 - increased level of GTP-bound protein

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Genotypes:

FYPO:0001038 - increased protein phosphorylation during vegetative growth

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0001883 - normal growth on caspofungin

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Genotypes:

FYPO:0000478 - normal meiosis

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Genotypes:

FYPO:0006008 - normal onset of protein localization to cell division site

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Genotypes:

FYPO:0004927 - normal prospore membrane formation

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Genotypes:

FYPO:0008003 - normal protein localization to site of mechanical stress

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Genotypes:

FYPO:0004422 - normal protein phosphorylation

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Genotypes:

FYPO:0003214 - normal protein phosphorylation during cellular response to glucose starvation

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Genotypes:

FYPO:0004154 - normal protein phosphorylation during cellular response to heat

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Genotypes:

FYPO:0000590 - normal sporulation

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0001310 - normal viability in stationary phase

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

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Genotypes:

FYPO:0006931 - sensitive to butylated hydroxytoluene

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0000280 - sterile

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Genotypes:

FYPO:0007436 - swollen elongated multiseptate vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

FYPO:0002104 - viable vegetative cell with normal cell shape

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00621RhoGEFDH_domPFAM
PF00610DEPDEP_domPFAM
PF15405PH_5PH_5PFAM
PF00780CNHCNH_domPFAM
cd00160RhoGEFDH_domCDD
cd04435DEP_fRom2CDD
PS50010DH_2DH_domPROSITE_PROFILES
PS50219CNHCNH_domPROSITE_PROFILES
SM00049DEP_3DEP_domSMART
SM00325RhoGEF_3DH_domSMART
SM00036nik_2CNH_domSMART
G3DSA:2.30.29.30:FF:000405FUNFAM
SSF48065DBL homology domain (DH-domain)DBL_dom_sfSUPERFAMILY
SSF46785"Winged helix" DNA-binding domainWH_DNA-bd_sfSUPERFAMILY
G3DSA:1.20.900.10Dbl homology (DH) domainDBL_dom_sfGENE3D
G3DSA:2.30.29.30PH-like_dom_sfGENE3D
G3DSA:1.10.10.10WH-like_DNA-bd_sfGENE3D
PTHR46572RHO1 GDP-GTP EXCHANGE PROTEIN 1-RELATEDRho-type_GEFsPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity
mobidb-lite-Polardisorder_predictionMOBIDB-Polar

Orthologs

References / Literature

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:19037094 - The Rho1p exchange factor Rgf1p signals upstream from the Pmk1 mitogen-activated protein kinase pathway in fission yeast.
Garcia P et al. Mol Biol Cell 2009 Jan;20(2):721-31
PMID:23907979 - The fission yeast cell wall stress sensor-like proteins Mtl2 and Wsc1 act by turning on the GTPase Rho1p but act independently of the cell wall integrity pathway.
Cruz S et al. Microbiologyopen 2013 Oct;2(5):778-94
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24478458 - The checkpoint-dependent nuclear accumulation of Rho1p exchange factor Rgf1p is important for tolerance to chronic replication stress.
Muñoz S et al. Mol Biol Cell 2014 Apr;25(7):1137-50
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:20625380 - A genome-wide screen for Schizosaccharomyces pombe deletion mutants that affect telomere length.
Liu NN et al. Cell Res 2010 Aug;20(8):963-5
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:37039135 - Cdc42 prevents precocious Rho1 activation during cytokinesis in a Pak1-dependent manner.
Onwubiko UN et al. J Cell Sci 2023 Apr 15;136(8)
PMID:34666001 - Detection of surface forces by the cell-wall mechanosensor Wsc1 in yeast.
Neeli-Venkata R et al. Dev Cell 2021 Oct 25;56(20):2856-2870.e7
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:16324155 - Rho1-GEFs Rgf1 and Rgf2 are involved in formation of cell wall and septum, while Rgf3 is involved in cytokinesis in fission yeast.
Mutoh T et al. Genes Cells 2005 Dec;10(12):1189-202
PMID:16291723 - Cell wall remodeling at the fission yeast cell division site requires the Rho-GEF Rgf3p.
Morrell-Falvey JL et al. J Cell Sci 2005 Dec 01;118(Pt 23):5563-73
PMID:20739711 - Cooperation between the septins and the actomyosin ring and role of a cell-integrity pathway during cell division in fission yeast.
Wu JQ et al. Genetics 2010 Nov;186(3):897-915
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
GO_REF:0000002 - Comments
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:26644575 - Discovery of a nucleocytoplasmic O-mannose glycoproteome in yeast.
Halim A et al. Proc Natl Acad Sci U S A 2015 Dec 22;112(51):15648-53
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:19189958 - Fission yeast rgf2p is a rho1p guanine nucleotide exchange factor required for spore wall maturation and for the maintenance of cell integrity in the absence of rgf1p.
García P et al. Genetics 2009 Apr;181(4):1321-34