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protein coding gene - klf1 (SPAC1039.05c) - DNA-binding transcription factor, zf-fungal binuclear cluster type Klf1

Gene summary

Standard name
klf1
Systematic ID
SPAC1039.05c
Product
DNA-binding transcription factor, zf-fungal binuclear cluster type Klf1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9US36
ORFeome ID
38/38G08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 5456674..5459561 reverse strand

Annotation

GO biological process

GO:0000122 - negative regulation of transcription by RNA polymerase II

References:

GO:0045944 - positive regulation of transcription by RNA polymerase II

References:

GO:0060237 - regulation of fungal-type cell wall organization

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0072686 - mitotic spindle

References:

GO:0005730 - nucleolus

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0001228 - DNA-binding transcription activator activity, RNA polymerase II-specific

References:

GO:0000978 - RNA polymerase II cis-regulatory region sequence-specific DNA binding

References:

GO:0008270 - zinc ion binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

Multi-locus phenotype

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

Protein features

PBO:0111745 - zf-C2H2 type

PBO:0111743 - zinc finger protein

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000012 - RNA level decreased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0002955 - abnormal G0 to G1 transition

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Genotypes:

FYPO:0004276 - abnormal negative regulation of transcription during G0

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Genotypes:

FYPO:0004275 - abnormal positive regulation of transcription during G0

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Genotypes:

FYPO:0000046 - decreased cell population growth

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Genotypes:

FYPO:0002944 - decreased RNA level during G0

References:

Genotypes:

FYPO:0002961 - delaminated cell wall during G0

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Genotypes:

FYPO:0002951 - increased L-ergothioneine level during G0

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Genotypes:

FYPO:0002950 - increased N-acetyl-D-glucosaminate level during G0

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Genotypes:

FYPO:0002945 - increased RNA level during G0

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Genotypes:

FYPO:0002954 - increased S-adenosyl-3-thiopropylamine level during G0

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Genotypes:

FYPO:0002953 - increased S-adenosyl-L-methionine level during G0

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Genotypes:

FYPO:0002952 - increased S-methyl-L-ergothioneine level during G0

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0006518 - loss of viability in G0

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Genotypes:

FYPO:0001178 - loss of viability upon nitrogen starvation

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Genotypes:

FYPO:0007553 - normal G1 to G0 transition

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Genotypes:

FYPO:0004099 - normal mitotic chromosome condensation

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Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0002578 - resistance to hydroxyurea

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002942 - swollen spheroid cell during G0

References:

Genotypes:

FYPO:0002943 - swollen spheroid cell during G0 to G1 transition

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

Protein features

IDNameInterPro nameDB name
PF04082Fungal_transXnlR_reg_domPFAM
PF00096zf-C2H2Znf_C2H2_typePFAM
cd12148fungal_TF_MHRCDD
PS00028ZINC_FINGER_C2H2_1Znf_C2H2_typePROSITE_PATTERNS
PS50157ZINC_FINGER_C2H2_2Znf_C2H2_typePROSITE_PROFILES
SM00355c2h2final6Znf_C2H2_typeSMART
G3DSA:3.30.160.60:FF:005292FUNFAM
SSF57667beta-beta-alpha zinc fingersZnf_C2H2_sfSUPERFAMILY
G3DSA:3.30.160.60Classic Zinc FingerGENE3D
PTHR40626MIP31509PVerF-likePANTHER

Orthologs

References / Literature

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:23695302 - Functional characterization of fission yeast transcription factors by overexpression analysis.
Vachon L et al. Genetics 2013 Aug;194(4):873-84
PMID:26896847 - Ensembl comparative genomics resources.
Herrero J et al. Database (Oxford) 2016;2016
GO_REF:0000002 - Comments
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:29735745 - Control of mitotic chromosome condensation by the fission yeast transcription factor Zas1.
Schiklenk C et al. J Cell Biol 2018 Jul 02;217(7):2383-2401
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.
Li J et al. Nat Commun 2019 Mar 01;10(1):1002
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:35924983 - Genetic-interaction screens uncover novel biological roles and regulators of transcription factors in fission yeast.
Chatfield-Reed K et al. G3 (Bethesda) 2022 Aug 25;12(9)
PMID:24167631 - Klf1, a C2H2 zinc finger-transcription factor, is required for cell wall maintenance during long-term quiescence in differentiated G0 phase.
Shimanuki M et al. PLoS One 2013;8(10):e78545
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
GO_REF:0000050 - Manual transfer of GO annotation data to genes by curator judgment of sequence model
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:39747188 - PhpC NF-Y transcription factor infiltrates heterochromatin to generate cryptic intron-containing transcripts crucial for small RNA production.
Srivastav MK et al. Nat Commun 2025 Jan 02;16(1):268
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:24385927 - The PAF complex and Prf1/Rtf1 delineate distinct Cdk9-dependent pathways regulating transcription elongation in fission yeast.
Mbogning J et al. PLoS Genet 2013;9(12):e1004029