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protein coding gene - pma1 (SPAC1071.10c) - plasma membrane P-type proton exporting ATPase, P3-type Pma1

Gene summary

Standard name
pma1
Systematic ID
SPAC1071.10c
Product
plasma membrane P-type proton exporting ATPase, P3-type Pma1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
P09627
ORFeome ID
39/39A12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3871642..3876111 reverse strand

Annotation

Complementation

PBO:0012247 - functionally complements S. cerevisiae pma1

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GO biological process

GO:0120029 - proton export across plasma membrane

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GO:0051453 - regulation of intracellular pH

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GO cellular component

GO:0005737 - cytoplasm

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GO:0005794 - Golgi apparatus

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GO:0016020 - membrane

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GO:0005886 - plasma membrane

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GO molecular function

GO:0005524 - ATP binding

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GO:0016887 - ATP hydrolysis activity

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GO:0008553 - P-type proton-exporting transporter activity

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GO:1901691 - proton binding

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

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MOD:00696 - phosphorylated residue

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MOD:01149 - sumoylated lysine

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0004085 - decreased vegetative cell growth

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Genotypes:

Protein sequence feature

SO:0001527 - peptide_localization_signal

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SO:0001812 - transmembrane_helix

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Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001360 - decreased amino acid import during vegetative growth

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Genotypes:

FYPO:0001168 - decreased ATPase activity

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Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

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Genotypes:

FYPO:0004874 - decreased glucose consumption

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Genotypes:

FYPO:0003647 - decreased sorbose import

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Genotypes:

FYPO:0004085 - decreased vegetative cell growth

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0001890 - increased RNA level

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

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Genotypes:

FYPO:0002151 - inviable spore

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0001158 - normal cellular pH

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Genotypes:

FYPO:0001241 - normal growth on anisomycin

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Genotypes:

FYPO:0001437 - normal growth on antimycin A

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Genotypes:

FYPO:0001439 - normal growth on chloramphenicol

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Genotypes:

FYPO:0001236 - normal growth on cycloheximide

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Genotypes:

FYPO:0001435 - normal growth on erythromycin

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Genotypes:

FYPO:0000979 - normal growth on miconazole

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Genotypes:

FYPO:0001438 - normal growth on tetracycline

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Genotypes:

FYPO:0001436 - normal growth on trichodermin

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Genotypes:

FYPO:0007098 - normal growth on vanadate

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Genotypes:

FYPO:0004083 - normal protein level

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Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

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Genotypes:

FYPO:0000840 - normal RNA level

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Genotypes:

FYPO:0001420 - normal vegetative cell population growth rate

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Genotypes:

FYPO:0001310 - normal viability in stationary phase

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Genotypes:

FYPO:0001433 - resistance to decamethylenediguanidine

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Genotypes:

FYPO:0001431 - resistance to Dio-9

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Genotypes:

FYPO:0001432 - resistance to ethidium bromide

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Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

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Genotypes:

FYPO:0001434 - resistance to N,N'-(p-xylylidene)bis-aminoguanidine 2HCl

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Genotypes:

FYPO:0000830 - resistance to vanadate

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Genotypes:

FYPO:0000722 - sensitive to acidity

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Genotypes:

FYPO:0001488 - sensitive to protamine sulfate

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

Protein features

IDNameInterPro nameDB name
PF00690Cation_ATPase_NATPase_P-typ_cation-transptr_NPFAM
PF00702HydrolasePFAM
PF00122E1-E2_ATPaseATPase_P-type_domAPFAM
cd02076P-type_ATPase_HP-type_ATPase_IIIACDD
PS00154ATPASE_E1_E2ATPase_P-typ_P_sitePROSITE_PATTERNS
SM00831Cation_ATPase_N_a_2ATPase_P-typ_cation-transptr_NSMART
PR00120HATPASEP_typ_ATPasePRINTS
PR00119CATATPASEPRINTS
G3DSA:3.40.1110.10:FF:000005FUNFAM
G3DSA:2.70.150.10:FF:000011FUNFAM
G3DSA:3.40.50.1000:FF:000008FUNFAM
SSF81665Calcium ATPase, transmembrane domain MATPase_P-typ_TM_dom_sfSUPERFAMILY
SSF81653Calcium ATPase, transduction domain AATPase_P-typ_transduc_dom_A_sfSUPERFAMILY
SSF56784HAD-likeHAD-like_sfSUPERFAMILY
G3DSA:1.20.1110.10GENE3D
G3DSA:3.40.1110.10ATPase_P-typ_cyto_dom_NGENE3D
G3DSA:3.40.50.1000HAD_sfGENE3D
G3DSA:2.70.150.10GENE3D
PTHR42861CALCIUM-TRANSPORTING ATPASEPANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Negative-Polyelectrolytedisorder_predictionMOBIDB-Negative-Polyelectrolyte
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte
TIGR01647ATPase-IIIA_HP-type_ATPase_IIIANCBIFAM
TIGR01494ATPase_P-typeP_typ_ATPaseNCBIFAM
SFLDF00027p-type_atpaseP_typ_ATPase_HD_domSFLD
SFLDS00003Haloacid_DehalogenaseSFLD
SFLDG00002C1.7:_P-type_atpase_likeSFLD

Orthologs

References / Literature

PMID:31294478 - Senataxin homologue Sen1 is required for efficient termination of RNA polymerase III transcription.
Rivosecchi J et al. EMBO J 2019 Aug 15;38(16):e101955
PMID:25240800 - The RNA exosome promotes transcription termination of backtracked RNA polymerase II.
Lemay JF et al. Nat Struct Mol Biol 2014 Oct;21(10):919-26
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:2891694 - Mutation of a conserved glycine residue modifies the vanadate sensitivity of the plasma membrane H+-ATPase from Schizosaccharomyces pombe.
Ghislain M et al. J Biol Chem 1987 Dec 25;262(36):17549-55
PMID:26404184 - High Confidence Fission Yeast SUMO Conjugates Identified by Tandem Denaturing Affinity Purification.
Nie M et al. Sci Rep 2015 Sep 25;5:14389
PMID:1532582 - Purification and complete sequence of a small proteolipid associated with the plasma membrane H(+)-ATPase of Saccharomyces cerevisiae.
Navarre C et al. J Biol Chem 1992 Mar 25;267(9):6425-8
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:38269097 - Rapamycin-sensitive mechanisms confine the growth of fission yeast below the temperatures detrimental to cell physiology.
Morozumi Y et al. iScience 2024 Jan 19;27(1):108777
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:36799444 - Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast.
Hirano Y et al. J Biochem 2023 Jun 30;174(1):33-46
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:39358553 - Ageing-associated long non-coding RNA extends lifespan and reduces translation in non-dividing cells.
Anver S et al. EMBO Rep 2024 Oct 02;
PMID:40484900 - Characterization of P-type H + -ATPase Pma1 inhibitors that extend chronological lifespan in fission yeast.
Tamura M et al. Mol Genet Genomics 2025 Jun 08;300(1):58
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:21832151 - Endocytosis is essential for dynamic translocation of a syntaxin 1 orthologue during fission yeast meiosis.
Kashiwazaki J et al. Mol Biol Cell 2011 Oct;22(19):3658-70
GO_REF:0000002 - Comments
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:26167880 - SR protein kinases promote splicing of nonconsensus introns.
Lipp JJ et al. Nat Struct Mol Biol 2015 Aug;22(8):611-7
PMID:2832071 - A new mutation for multiple drug resistance and modified plasma membrane ATPase activity in Schizosaccharomyces pombe.
Ulaszewski S et al. Curr Genet 1986;10(5):359-64
PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.
Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663
PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.
Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99
PMID:1833395 - The pma1 and pma2 H(+)-ATPases from Schizosaccharomyces pombe are functionally interchangeable.
Ghislain M et al. J Biol Chem 1991 Sep 25;266(27):18276-9
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:26896847 - Ensembl comparative genomics resources.
Herrero J et al. Database (Oxford) 2016;2016
PMID:2878925 - A single mutation confers vanadate resistance to the plasma membrane H+-ATPase from the yeast Schizosaccharomyces pombe.
Ulaszewski S et al. J Biol Chem 1987 Jan 05;262(1):223-8
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:1310980 - The yeast plasma membrane H(+)-ATPase. An essential change of conformation triggered by H+.
Blanpain JP et al. J Biol Chem 1992 Feb 25;267(6):3735-40
PMID:1396704 - Altered plasma membrane H(+)-ATPase from the Dio-9-resistant pma1-2 mutant of Schizosaccharomyces pombe.
Ghislain M et al. Eur J Biochem 1992 Oct 01;209(1):275-9
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.
Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.
Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.
Larochelle M et al. J Cell Sci 2019 May 31;132(11)
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:27974503 - Traffic Through the Trans-Golgi Network and the Endosomal System Requires Collaboration Between Exomer and Clathrin Adaptors in Fission Yeast.
Hoya M et al. Genetics 2017 Feb;205(2):673-690
PMID:25379379 - A new pma1 mutation identified in a chronologically long-lived fission yeast mutant.
Naito C et al. FEBS Open Bio 2014;4:829-33
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.
Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671
PMID:39110593 - VAP-mediated membrane-tethering mechanisms implicate ER-PM contact function in pH homeostasis.
Hoh KL et al. Cell Rep 2024 Aug 05;43(8):114592
PMID:20829365 - Pma1, a P-type proton ATPase, is a determinant of chronological life span in fission yeast.
Ito H et al. J Biol Chem 2010 Nov 05;285(45):34616-20
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;