protein coding gene - cdc42 (SPAC110.03) - Rho family GTPase Cdc42

Gene summary

Standard name

cdc42

Systematic ID

SPAC110.03

Product

Rho family GTPase Cdc42

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

Q01112

ORFeome ID

50/50D07

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome I: 1916009..1917656 forward strand

Annotation

Protein features

Orthologs

• CDC42 (HGNC:1736) Homo sapiens
• cdc42 (SJAG_04509) Schizosaccharomyces japonicus
• CDC42 (YLR229C) Saccharomyces cerevisiae

References / Literature

PMID:25837586 - Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based trafficking.

Bendezú FO et al. PLoS Biol 2015 Apr;13(4):e1002097

PMID:12415007 - The small GTPase Rho3 and the diaphanous/formin For3 function in polarized cell growth in fission yeast.

Nakano K et al. J Cell Sci 2002 Dec 01;115(Pt 23):4629-39

PMID:31152053 - Molecular mechanisms of chemotropism and cell fusion in unicellular fungi.

Martin SG J Cell Sci 2019 May 31;132(11)

PMID:27852900 - Gradients of phosphatidylserine contribute to plasma membrane charge localization and cell polarity in fission yeast.

Haupt A et al. Mol Biol Cell 2017 Jan 01;28(1):210-220

PMID:10567532 - Direct binding and In vivo regulation of the fission yeast p21-activated kinase shk1 by the SH3 domain protein scd2.

Chang E et al. Mol Cell Biol 1999 Dec;19(12):8066-74

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:19646873 - The conserved NDR kinase Orb6 controls polarized cell growth by spatial regulation of the small GTPase Cdc42.

Das M et al. Curr Biol 2009 Aug 11;19(15):1314-9

PMID:32179107 - Pattern formation in a coupled membrane-bulk reaction-diffusion model for intracellular polarization and oscillations.

Paquin-Lefebvre F et al. J Theor Biol 2020 Jul 21;497:110242

PMID:8943016 - The highly conserved skb1 gene encodes a protein that interacts with Shk1, a fission yeast Ste20/PAK homolog.

Gilbreth M et al. Proc Natl Acad Sci U S A 1996 Nov 26;93(24):13802-7

PMID:29930085 - Local and global Cdc42 guanine nucleotide exchange factors for fission yeast cell polarity are coordinated by microtubules and the Tea1-Tea4-Pom1 axis.

Tay YD et al. J Cell Sci 2018 Jul 19;131(14)

PMID:15546915 - Schizosaccharomyces pombe Rgf3p is a specific Rho1 GEF that regulates cell wall beta-glucan biosynthesis through the GTPase Rho1p.

Tajadura V et al. J Cell Sci 2004 Dec 01;117(Pt 25):6163-74

PMID:14573463 - Schizosaccharomyces pombe Ras1 effector, Scd1, interacts with Klp5 and Klp6 kinesins to mediate cytokinesis.

Li Y et al. Genetics 2003 Oct;165(2):477-88

PMID:23615447 - Compartmentalized nodes control mitotic entry signaling in fission yeast.

Deng L et al. Mol Biol Cell 2013 Jun;24(12):1872-81

PMID:21401840 - Pob1 ensures cylindrical cell shape by coupling two distinct rho signaling events during secretory vesicle targeting.

Nakano K et al. Traffic 2011 Jun;12(6):726-39

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:20870879 - Reorganization of the growth pattern of Schizosaccharomyces pombe in invasive filament formation.

Dodgson J et al. Eukaryot Cell 2010 Nov;9(11):1788-97

PMID:21849474 - Spatial control of Cdc42 activation determines cell width in fission yeast.

Kelly FD et al. Mol Biol Cell 2011 Oct;22(20):3801-11

PMID:40187481 - Where to grow and where to go.

Kriegler M et al. Fungal Genet Biol 2025 Apr 03;:103983

PMID:8846783 - Fission yeast pak1+ encodes a protein kinase that interacts with Cdc42p and is involved in the control of cell polarity and mating.

Ottilie S et al. EMBO J 1995 Dec 01;14(23):5908-19

PMID:23060961 - Cdc42 regulation of polarized traffic in fission yeast.

Estravis M et al. Commun Integr Biol 2012 Jul 01;5(4):370-3

PMID:12409291 - The Cdc42 binding and scaffolding activities of the fission yeast adaptor protein Scd2.

Endo M et al. J Biol Chem 2003 Jan 10;278(2):843-52

PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.

UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531

PMID:24947517 - The putative exchange factor Gef3p interacts with Rho3p GTPase and the septin ring during cytokinesis in fission yeast.

Muñoz S et al. J Biol Chem 2014 Aug 08;289(32):21995-2007

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:17363901 - Hob3p, the fission yeast ortholog of human BIN3, localizes Cdc42p to the division site and regulates cytokinesis.

Coll PM et al. EMBO J 2007 Apr 04;26(7):1865-77

PMID:26960792 - Rga6 is a Fission Yeast Rho GAP Involved in Cdc42 Regulation of Polarized Growth.

Revilla-Guarinos MT et al. Mol Biol Cell 2016 Mar 09;27(9):1524-35

PMID:18256290 - Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis.

Pinar M et al. Mol Biol Cell 2008 Apr;19(4):1727-38

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:34523683 - Cdc42 reactivation at growth sites is regulated by local cell-cycle-dependent loss of its GTPase-activating protein Rga4 in fission yeast.

Rich-Robinson J et al. J Cell Sci 2021 Oct 01;134(19)

PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.

Mata J et al. Nat Genet 2002 Sep;32(1):143-7

PMID:12529446 - Gef1p, a new guanine nucleotide exchange factor for Cdc42p, regulates polarity in Schizosaccharomyces pombe.

Coll PM et al. Mol Biol Cell 2003 Jan;14(1):313-23

PMID:22768263 - Fission yeast Sec3 and Exo70 are transported on actin cables and localize the exocyst complex to cell poles.

Bendezú FO et al. PLoS One 2012;7(6):e40248

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:26941334 - Unique spatiotemporal activation pattern of Cdc42 by Gef1 and Scd1 promotes different events during cytokinesis.

Wei B et al. Mol Biol Cell 2016 Apr 15;27(8):1235-45

PMID:9858584 - Genetic evidence for Pak1 autoinhibition and its release by Cdc42.

Tu H et al. Mol Cell Biol 1999 Jan;19(1):602-11

PMID:10628977 - Isolation and characterization of Nrf1p, a novel negative regulator of the Cdc42p GTPase in Schizosaccharomyces pombe.

Murray JM et al. Genetics 2000 Jan;154(1):155-65

PMID:21148300 - Actin cables and the exocyst form two independent morphogenesis pathways in the fission yeast.

Bendezú FO et al. Mol Biol Cell 2011 Jan 01;22(1):44-53

PMID:9892665 - Moe1, a conserved protein in Schizosaccharomyces pombe, interacts with a Ras effector, Scd1, to affect proper spindle formation.

Chen CR et al. Proc Natl Acad Sci U S A 1999 Jan 19;96(2):517-22

PMID:9660817 - Cloning and characterization of shk2, a gene encoding a novel p21-activated protein kinase from fission yeast.

Yang P et al. J Biol Chem 1998 Jul 17;273(29):18481-9

PMID:28821619 - Dsc E3 ligase localization to the Golgi requires the ATPase Cdc48 and cofactor Ufd1 for activation of sterol regulatory element-binding protein in fission yeast.

Burr R et al. J Biol Chem 2017 Sep 29;292(39):16333-16350

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:22891673 - Fission yeast sec3 bridges the exocyst complex to the actin cytoskeleton.

Jourdain I et al. Traffic 2012 Nov;13(11):1481-95

PMID:36068165 - Cdc42 GTPase activating proteins Rga4 and Rga6 coordinate septum synthesis and membrane trafficking at the division plane during cytokinesis.

Campbell BF et al. Traffic 2022 Oct;23(10):478-495

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:25411334 - The Rho-GEF Gef3 interacts with the septin complex and activates the GTPase Rho4 during fission yeast cytokinesis.

Wang N et al. Mol Biol Cell 2015 Jan 15;26(2):238-55

PMID:19710424 - Pob1 participates in the Cdc42 regulation of fission yeast actin cytoskeleton.

Rincón SA et al. Mol Biol Cell 2009 Oct;20(20):4390-9

PMID:21899677 - Cdc42 regulates multiple membrane traffic events in fission yeast.

Estravís M et al. Traffic 2011 Dec;12(12):1744-58

PMID:18328707 - Pom1 DYRK regulates localization of the Rga4 GAP to ensure bipolar activation of Cdc42 in fission yeast.

Tatebe H et al. Curr Biol 2008 Mar 11;18(5):322-30

PMID:14637153 - Role of guanine nucleotide exchange factors for Rho family GTPases in the regulation of cell morphology and actin cytoskeleton in fission yeast.

Iwaki N et al. Biochem Biophys Res Commun 2003 Dec 12;312(2):414-20

PMID:10593886 - Direct activation of the fission yeast PAK Shk1 by the novel SH3 domain protein, Skb5.

Yang P et al. J Biol Chem 1999 Dec 17;274(51):36052-7

PMID:1587480 - A homologue of the ras-related CDC42 gene from Schizosaccharomyces pombe.

Fawell E et al. Gene 1992 May 01;114(1):153-4

PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.

Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31

PMID:24554432 - The Tea4-PP1 landmark promotes local growth by dual Cdc42 GEF recruitment and GAP exclusion.

Kokkoris K et al. J Cell Sci 2014 May 01;127(Pt 9):2005-16

PMID:25422470 - Oscillatory AAA+ ATPase Knk1 constitutes a novel morphogenetic pathway in fission yeast.

Scheffler K et al. Proc Natl Acad Sci U S A 2014 Dec 16;111(50):17899-904

PMID:30044717 - Roles of the fission yeast UNC-13/Munc13 protein Ync13 in late stages of cytokinesis.

Zhu YH et al. Mol Biol Cell 2018 Sep 15;29(19):2259-2279

PMID:30989357 - Functional interaction between Cdc42 and the stress MAPK signaling pathway during the regulation of fission yeast polarized growth.

Pérez P et al. Int Microbiol 2020 Jan;23(1):31-41

PMID:10961446 - The Cdc42p GTPase is targeted to the site of cell division in the fission yeast Schizosaccharomyces pombe.

Merla A et al. Eur J Cell Biol 2000 Jul;79(7):469-77

PMID:23200991 - Cdc42 explores the cell periphery for mate selection in fission yeast.

Bendezú FO et al. Curr Biol 2013 Jan 07;23(1):42-7

PMID:24146635 - Hsp70-Hsp40 chaperone complex functions in controlling polarized growth by repressing Hsf1-driven heat stress-associated transcription.

Vjestica A et al. PLoS Genet 2013;9(10):e1003886

PMID:31719163 - A novel interplay between GEFs orchestrates Cdc42 activity during cell polarity and cytokinesis in fission yeast.

Hercyk BS et al. J Cell Sci 2019 Dec 03;132(23)

PMID:12972551 - Gef1p and Scd1p, the Two GDP-GTP exchange factors for Cdc42p, form a ring structure that shrinks during cytokinesis in Schizosaccharomyces pombe.

Hirota K et al. Mol Biol Cell 2003 Sep;14(9):3617-27

PMID:15631622 - Interactions between Cdc42 and the scaffold protein Scd2: requirement of SH3 domains for GTPase binding.

Wheatley E et al. Biochem J 2005 May 15;388(Pt 1):177-84

PMID:30279276 - Differential GAP requirement for Cdc42-GTP polarization during proliferation and sexual reproduction.

Gallo Castro D et al. J Cell Biol 2018 Dec 03;217(12):4215-4229

PMID:32182184 - Cdc42 promotes Bgs1 recruitment for septum synthesis and glucanase localization for cell separation during cytokinesis in fission yeast.

Onwubiko UN et al. Small GTPases 2021 Jul;12(4):257-264

PMID:11042180 - The Cdc42p GTPase and its regulators Nrf1p and Scd1p are involved in endocytic trafficking in the fission yeast Schizosaccharomyces pombe.

Murray JM et al. J Biol Chem 2001 Feb 02;276(5):3004-9

PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.

Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41

PMID:32932721 - Activation of Cdc42 GTPase upon CRY2-Induced Cortical Recruitment Is Antagonized by GAPs in Fission Yeast.

Lamas I et al. Cells 2020 Sep 12;9(9)

PMID:34731607 - Stress-dependent inhibition of polarized cell growth through unbalancing the GEF/GAP regulation of Cdc42.

Salat-Canela C et al. Cell Rep 2021 Nov 02;37(5):109951

PMID:35773059 - Stress-induced cell depolarization through the MAP kinase-Cdc42 axis.

Salat-Canela C et al. Trends Cell Biol 2023 Feb;33(2):124-137

PMID:16421249 - Rgf1p is a specific Rho1-GEF that coordinates cell polarization with cell wall biogenesis in fission yeast.

García P et al. Mol Biol Cell 2006 Apr;17(4):1620-31

PB_REF:0000001 - Protein modification annotation by manual transfer of experimentally-verified annotation data to orthologs based on curator judgment of sequence features.

PMID:25500221 - Balance between exocytosis and endocytosis determines the efficacy of sterol-targeting antibiotics.

Nishimura S et al. Chem Biol 2014 Dec 18;21(12):1690-9

PMID:31666919 - Rho Family GTPases in Fission Yeast Cytokinesis.

Hercyk B et al. Commun Integr Biol 2019;12(1):171-180

PMID:34200466 - The Multiple Functions of Rho GTPases in Fission Yeasts.

Vicente-Soler J et al. Cells 2021 Jun 07;10(6)

PMID:39333500 - Cdc42 mobility and membrane flows regulate fission yeast cell shape and survival.

Rutkowski DM et al. Nat Commun 2024 Sep 27;15(1):8363

PMID:18793338 - Rga2 is a Rho2 GAP that regulates morphogenesis and cell integrity in S. pombe.

Villar-Tajadura MA et al. Mol Microbiol 2008 Nov;70(4):867-81

PMID:11453249 - The Schizosaccharomyces pombe Cdc42p GTPase signals through Pak2p and the Mkh1p-Pek1p-Spm1p MAP kinase pathway.

Merla A et al. Curr Genet 2001 Jun;39(4):205-9

PMID:24147005 - The coordination of cell growth during fission yeast mating requires Ras1-GTP hydrolysis.

Weston C et al. PLoS One 2013;8(10):e77487

PMID:39156640 - Fission yeast Bgs1 glucan synthase participates in the control of growth polarity and membrane traffic.

Ramos M et al. iScience 2024 Aug 16;27(8):110477

PMID:31591131 - F-BAR Cdc15 Promotes Cdc42 Activation During Cytokinesis and Cell Polarization in Schizosaccharomyces pombe .

Hercyk BS et al. Genetics 2019 Dec;213(4):1341-1356

PMID:8289788 - Cdc42p GTPase is involved in controlling polarized cell growth in Schizosaccharomyces pombe.

Miller PJ et al. Mol Cell Biol 1994 Feb;14(2):1075-83

PMID:7597098 - Shk1, a homolog of the Saccharomyces cerevisiae Ste20 and mammalian p65PAK protein kinases, is a component of a Ras/Cdc42 signaling module in the fission yeast Schizosaccharomyces pombe.

Marcus S et al. Proc Natl Acad Sci U S A 1995 Jun 20;92(13):6180-4

PMID:9660818 - Characterization of Pak2p, a pleckstrin homology domain-containing, p21-activated protein kinase from fission yeast.

Sells MA et al. J Biol Chem 1998 Jul 17;273(29):18490-8

PMID:25469537 - The interdependence of the Rho GTPases and apicobasal cell polarity.

Mack NA et al. Small GTPases 2014;5(2):10

PMID:27746023 - Remodeling of the Fission Yeast Cdc42 Cell-Polarity Module via the Sty1 p38 Stress-Activated Protein Kinase Pathway.

Mutavchiev DR et al. Curr Biol 2016 Nov 07;26(21):2921-2928

PMID:8887550 - Rho 1 GTPase activates the (1-3)beta-D-glucan synthase and is involved in Schizosaccharomyces pombe morphogenesis.

Arellano M et al. EMBO J 1996 Sep 02;15(17):4584-91

PMID:9524252 - Isolation of a novel heat shock protein 70-like gene, pss1+ of Schizosaccharomyces pombe homologous to hsp110/SSE subfamily.

Chung KS et al. Gene 1998 Mar 27;210(1):143-50

PMID:10504305 - Schizosaccharomyces pombe protein kinase C homologues, pck1p and pck2p, are targets of rho1p and rho2p and differentially regulate cell integrity.

Arellano M et al. J Cell Sci 1999 Oct;112 ( Pt 20):3569-78

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:20164182 - Rga4 modulates the activity of the fission yeast cell integrity MAPK pathway by acting as a Rho2 GTPase-activating protein.

Soto T et al. J Biol Chem 2010 Apr 09;285(15):11516-25

PMID:28742002 - Cdc42 activation state affects its localization and protein levels in fission yeast.

Estravís M et al. Microbiology (Reading) 2017 Aug;163(8):1156-1166

PMID:7923372 - Cooperative interaction of S. pombe proteins required for mating and morphogenesis.

Chang EC et al. Cell 1994 Oct 07;79(1):131-41

PMID:30709916 - A Cdc42 GEF, Gef1, through endocytosis organizes F-BAR Cdc15 along the actomyosin ring and promotes concentric furrowing.

Onwubiko UN et al. J Cell Sci 2019 Feb 28;132(5)

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:37039135 - Cdc42 prevents precocious Rho1 activation during cytokinesis in a Pak1-dependent manner.

Onwubiko UN et al. J Cell Sci 2023 Apr 15;136(8)

PMID:39012625 - Arp2/3-dependent endocytosis ensures Cdc42 oscillations by removing Pak1-mediated negative feedback.

Harrell MA et al. J Cell Biol 2024 Sep 02;223(10)

PMID:29290560 - ER-PM Contacts Restrict Exocytic Sites for Polarized Morphogenesis.

Ng AYE et al. Curr Biol 2018 Jan 08;28(1):146-153.e5