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protein coding gene - swi2 (SPAC1142.03c) - DNA recombination mediator Swi2

Gene summary

Standard name
swi2
Systematic ID
SPAC1142.03c
Product
DNA recombination mediator Swi2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPAC17G6.20c
UniProt ID
Q10668
ORFeome ID
46/46F03
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3629015..3631458 reverse strand

Annotation

Comment

PBO:0002148 - focus formation at heterochromatic regions, Swi5_dependent

References:

PBO:0002149 - focus formation at heterochromatic regions, Swi6_dependent

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GO biological process

GO:0007535 - donor selection

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GO:0007534 - gene conversion at mating-type locus

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GO cellular component

GO:0031934 - mating-type region heterochromatin

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GO:0005730 - nucleolus

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GO:0005634 - nucleus

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GO:0034974 - Swi5-Swi2 complex

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GO molecular function

GO:0005515 - protein binding

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GO:1990837 - sequence-specific double-stranded DNA binding

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Modification

MOD:00046 - O-phospho-L-serine

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Multi-locus phenotype

FYPO:0000468 - abnormal mating type switching

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Genotypes:

FYPO:0003450 - abolished protein localization to nucleus

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Genotypes:

FYPO:0000470 - decreased mating type switching

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Genotypes:

Protein features

PBO:0111888 - AT hook protein

Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000468 - abnormal mating type switching

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Genotypes:

FYPO:0000121 - abnormal sporulation

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Genotypes:

FYPO:0000469 - abolished mating type switching

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Genotypes:

FYPO:0008168 - abolished nuclear foci

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Genotypes:

FYPO:0008153 - abolished protein localization to heterochromatin at silent mating-type cassette during vegetative growth

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Genotypes:

FYPO:0000705 - abolished protein-protein interaction

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Genotypes:

FYPO:0003352 - decreased DNA double-strand break formation at mating-type locus

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Genotypes:

FYPO:0005018 - decreased double-stranded DNA binding

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0000470 - decreased mating type switching

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Genotypes:

FYPO:0003573 - decreased protein localization to heterochromatin at silent mating-type cassette

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Genotypes:

FYPO:0001645 - decreased protein-protein interaction

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Genotypes:

FYPO:0009077 - increased cell population growth on ethanol carbon source

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Genotypes:

FYPO:0000636 - increased cell population growth rate

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Genotypes:

FYPO:0001821 - increased number of heterothallic h+ cells

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Genotypes:

FYPO:0006518 - loss of viability in G0

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Genotypes:

FYPO:0002360 - normal chromatin silencing at centromere

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Genotypes:

FYPO:0002336 - normal chromatin silencing at silent mating-type cassette

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Genotypes:

FYPO:0003353 - normal DNA double-strand break formation at mating-type locus

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Genotypes:

FYPO:0007553 - normal G1 to G0 transition

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Genotypes:

FYPO:0008070 - normal histone H3-K9 methylation at centromere during vegetative growth

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Genotypes:

FYPO:0005865 - normal histone H3-K9 methylation at silent mating-type cassette during vegetative growth

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Genotypes:

FYPO:0004083 - normal protein level

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Genotypes:

FYPO:0000703 - normal protein-protein interaction

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Genotypes:

FYPO:0003530 - normal S-phase DNA damage checkpoint

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Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0002693 - resistance to diamide

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Genotypes:

FYPO:0001453 - resistance to ethanol

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Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

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Genotypes:

FYPO:0000725 - resistance to methyl methanesulfonate

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Genotypes:

FYPO:0001701 - sensitive to bortezomib

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0007931 - sensitive to egtazic acid

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0009798 - faster evolving duplicate

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
G3DSA:6.10.140.1020GENE3D
PTHR28527MATING-TYPE SWITCHING PROTEIN SWI2-RELATEDPANTHER
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Negative-Polyelectrolytedisorder_predictionMOBIDB-Negative-Polyelectrolyte
mobidb-lite-Polardisorder_predictionMOBIDB-Polar

Orthologs

References / Literature

PMID:39945308 - Characterization of the Swi6/HP1 binding motif in its partner protein reveals the basis for the functional divergence of the HP1 family proteins in fission yeast.
Oya T et al. FASEB J 2025 Feb 28;39(4):e70387
PMID:10716938 - Fission yeast switches mating type by a replication-recombination coupled process.
Arcangioli B et al. EMBO J 2000 Mar 15;19(6):1389-96
PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.
Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31
PMID:29852001 - New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.
Maki T et al. PLoS Genet 2018 May;14(5):e1007424
PMID:14663140 - Two different Swi5-containing protein complexes are involved in mating-type switching and recombination repair in fission yeast.
Akamatsu Y et al. Proc Natl Acad Sci U S A 2003 Dec 23;100(26):15770-5
PMID:15537537 - Heterochromatin regulates cell type-specific long-range chromatin interactions essential for directed recombination.
Jia S et al. Cell 2004 Nov 12;119(4):469-80
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:29610759 - Molecular signature of the imprintosome complex at the mating-type locus in fission yeast.
Raimondi C et al. Microb Cell 2018 Jan 16;5(4):169-183
PMID:19037101 - Mus81, Rhp51(Rad51), and Rqh1 form an epistatic pathway required for the S-phase DNA damage checkpoint.
Willis N et al. Mol Biol Cell 2009 Feb;20(3):819-33
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:6587363 - Genes required for initiation and resolution steps of mating-type switching in fission yeast.
Egel R et al. Proc Natl Acad Sci U S A 1984 Jun;81(11):3481-5
PMID:18045993 - RFCCtf18 and the Swi1-Swi3 complex function in separate and redundant pathways required for the stabilization of replication forks to facilitate sister chromatid cohesion in Schizosaccharomyces pombe.
Ansbach AB et al. Mol Biol Cell 2008 Feb;19(2):595-607
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24719968 - Switching genes in Schizosaccharomyces pombe.
Gutz H et al. Curr Genet 1985;9(5):325-31
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:40083061 - Heterochromatin Protein Swi6 Suppresses Aberrant Gene Conversion at mat Loci by Adjusting the Balance Between the Two Pathways of Swi2 and Rad57.
Fujioka T et al. Genes Cells 2025 Mar;30(2):e70012
PMID:9710635 - A novel function of the DNA repair gene rhp6 in mating-type silencing by chromatin remodeling in fission yeast.
Singh J et al. Mol Cell Biol 1998 Sep;18(9):5511-22
PMID:18354497 - The fission yeast homologue of CENP-B, Abp1, regulates directionality of mating-type switching.
Aguilar-Arnal L et al. EMBO J 2008 Apr 09;27(7):1029-38
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:36951094 - Fission yeast Swi2 designates cell-type specific donor and stimulates Rad51-driven strand exchange for mating-type switching.
Maki T et al. Nucleic Acids Res 2023 May 08;51(8):3869-3887
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:22042869 - A homolog of male sex-determining factor SRY cooperates with a transposon-derived CENP-B protein to control sex-specific directed recombination.
Matsuda E et al. Proc Natl Acad Sci U S A 2011 Nov 15;108(46):18754-9
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:17304215 - Fission yeast Swi5/Sfr1 and Rhp55/Rhp57 differentially regulate Rhp51-dependent recombination outcomes.
Akamatsu Y et al. EMBO J 2007 Mar 07;26(5):1352-62
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83