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protein coding gene - vps32 (SPAC1142.07c) - ESCRT III complex subunit Vps32

Gene summary

Standard name
vps32
Systematic ID
SPAC1142.07c
Product
ESCRT III complex subunit Vps32
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
snf7
UniProt ID
Q9P7F7
ORFeome ID
10/10A03
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome I: 3638557..3639776 reverse strand

Annotation

Disease association

MONDO:0011547 - cataract 31 multiple types

References:

GO biological process

GO:0045324 - late endosome to vacuole transport

References:

GO:0007084 - mitotic nuclear membrane reassembly

References:

GO:0043328 - protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway

References:

GO cellular component

GO:0005737 - cytoplasm

References:

GO:0009898 - cytoplasmic side of plasma membrane

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GO:0000815 - ESCRT III complex

References:

GO:0180028 - mitotic spindle pole body attachment site

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GO molecular function

GO:0005515 - protein binding

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Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01149 - sumoylated lysine

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0001972 - abnormal cell separation after cytokinesis resulting in septated cell

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Genotypes:

FYPO:0000272 - abolished septum assembly

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Genotypes:

FYPO:0000339 - mislocalized septum during vegetative growth

References:

Genotypes:

FYPO:0001390 - misoriented septum during vegetative growth

References:

Genotypes:

FYPO:0000118 - multiseptate vegetative cell

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Genotypes:

Qualitative gene expression

PomGeneEx:0000021 - protein present

References:

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001972 - abnormal cell separation after cytokinesis resulting in septated cell

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Genotypes:

FYPO:0003625 - abnormal microtubule cytoskeleton morphology during mitotic interphase

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Genotypes:

FYPO:0001894 - abnormal sporulation resulting in formation of ascus with more or fewer than four spores

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Genotypes:

FYPO:0004482 - abnormal vacuole fusion during vegetative growth

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Genotypes:

FYPO:0000272 - abolished septum assembly

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Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0000584 - decreased sporulation frequency

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0007475 - delayed onset of protein localization to mitotic spindle pole body

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Genotypes:

FYPO:0001475 - fragmented mitotic spindle pole body

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Genotypes:

FYPO:0001460 - increased transcription from CDRE promoter in response to calcium ion

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0000339 - mislocalized septum during vegetative growth

References:

Genotypes:

FYPO:0000118 - multiseptate vegetative cell

References:

Genotypes:

FYPO:0002343 - normal growth on terbinafine

References:

Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

References:

Genotypes:

FYPO:0001986 - resistance to 5-fluorouracil

References:

Genotypes:

FYPO:0006580 - resistance to amiodarone

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001103 - resistance to hydrogen peroxide

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0005969 - resistance to magnesium chloride

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0005968 - resistance to sodium chloride

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Genotypes:

FYPO:0005253 - resistance to tamoxifen

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Genotypes:

FYPO:0002642 - sensitive to amphotericin B

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0000098 - sensitive to calcium

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Genotypes:

FYPO:0000085 - sensitive to camptothecin

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Genotypes:

FYPO:0000101 - sensitive to chlorpropham

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Genotypes:

FYPO:0002640 - sensitive to clotrimazole

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Genotypes:

FYPO:0001245 - sensitive to cobalt

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0006581 - sensitive to fenpropimorph

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0006579 - sensitive to manganese

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Genotypes:

FYPO:0000751 - sensitive to nickel

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0002328 - sensitive to terbinafine

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Genotypes:

FYPO:0002380 - viable spheroid vegetative cell

References:

Genotypes:

FYPO:0002106 - viable stubby vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002197 - viable vegetative cell with abnormal cell shape

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF03357Snf7Snf7_famPFAM
G3DSA:1.10.287.1060:FF:000007FUNFAM
G3DSA:6.10.250.1710GENE3D
G3DSA:1.10.287.1060GENE3D
PTHR22761CHARGED MULTIVESICULAR BODY PROTEINSnf7_famPANTHER
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

Orthologs

References / Literature

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:22682253 - Functional repurposing revealed by comparing S. pombe and S. cerevisiae genetic interactions.
Frost A et al. Cell 2012 Jun 08;149(6):1339-52
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:21965289 - Nsk1 ensures accurate chromosome segregation by promoting association of kinetochores to spindle poles during anaphase B.
Buttrick GJ et al. Mol Biol Cell 2011 Dec;22(23):4486-502
PMID:19672306 - Genome-wide screen of genes required for caffeine tolerance in fission yeast.
Calvo IA et al. PLoS One 2009 Aug 12;4(8):e6619
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:19264558 - Screening a genome-wide S. pombe deletion library identifies novel genes and pathways involved in genome stability maintenance.
Deshpande GP et al. DNA Repair (Amst) 2009 May 01;8(5):672-9
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:22252817 - A genomewide screen in Schizosaccharomyces pombe for genes affecting the sensitivity of antifungal drugs that target ergosterol biosynthesis.
Fang Y et al. Antimicrob Agents Chemother 2012 Apr;56(4):1949-59
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:27918601 - Identifying genes required for respiratory growth of fission yeast.
Malecki M et al. Wellcome Open Res 2016;1:12
PMID:17951524 - Schizosaccharomyces pombe Sst4p, a conserved Vps27/Hrs homolog, functions downstream of phosphatidylinositol 3-kinase Pik3p to mediate proper spore formation.
Onishi M et al. Eukaryot Cell 2007 Dec;6(12):2343-53
PMID:26628015 - Characterization of Tamoxifen as an Antifungal Agent Using the Yeast Schizosaccharomyces Pombe Model Organism.
Zhang X et al. Kobe J Med Sci 2015 Oct 09;61(2):E54-63
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
GO_REF:0000051 - S. pombe keyword mapping
PMID:29856841 - Genome-wide screen reveals important roles for ESCRT proteins in drug/ion resistance of fission yeast.
Yang Y et al. PLoS One 2018;13(6):e0198516
PMID:37783794 - An ESCRT grommet cooperates with a diffusion barrier to maintain nuclear integrity.
Ader NR et al. Nat Cell Biol 2023 Oct;25(10):1465-1477
PMID:25373780 - A genomic Multiprocess survey of machineries that control and link cell shape, microtubule organization, and cell-cycle progression.
Graml V et al. Dev Cell 2014 Oct 27;31(2):227-239
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:27053105 - Mga2 Transcription Factor Regulates an Oxygen-responsive Lipid Homeostasis Pathway in Fission Yeast.
Burr R et al. J Biol Chem 2016 Jun 03;291(23):12171-83
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:17660439 - Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe.
Iwaki T et al. Microbiology (Reading) 2007 Aug;153(Pt 8):2753-2764
PMID:25356547 - A complex network of interactions between mitotic kinases, phosphatases and ESCRT proteins regulates septation and membrane trafficking in S. pombe.
Bhutta MS et al. PLoS One 2014;9(10):e111789